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O35633

- VIAAT_MOUSE

UniProt

O35633 - VIAAT_MOUSE

Protein

Vesicular inhibitory amino acid transporter

Gene

Slc32a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 3 (28 Mar 2003)
      Previous versions | rss
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    Functioni

    Involved in the uptake of GABA and glycine into the synaptic vesicles.

    GO - Molecular functioni

    1. gamma-aminobutyric acid:proton symporter activity Source: MGI
    2. glycine transmembrane transporter activity Source: MGI

    GO - Biological processi

    1. aging Source: Ensembl
    2. gamma-aminobutyric acid transport Source: GOC
    3. glycine transport Source: MGI
    4. neurotransmitter transport Source: UniProtKB-KW

    Keywords - Biological processi

    Neurotransmitter transport, Transport

    Protein family/group databases

    TCDBi2.A.18.5.3. the amino acid/auxin permease (aaap) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vesicular inhibitory amino acid transporter
    Alternative name(s):
    GABA and glycine transporter
    Solute carrier family 32 member 1
    Vesicular GABA transporter
    Short name:
    mVGAT
    Short name:
    mVIAAT
    Gene namesi
    Name:Slc32a1
    Synonyms:Vgat, Viaat
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:1194488. Slc32a1.

    Subcellular locationi

    GO - Cellular componenti

    1. cell tip Source: MGI
    2. cone cell pedicle Source: MGI
    3. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
    4. dendrite Source: MGI
    5. dendrite terminus Source: MGI
    6. inhibitory synapse Source: MGI
    7. integral component of plasma membrane Source: MGI
    8. neuron projection Source: MGI
    9. neuron projection terminus Source: MGI
    10. synapse Source: MGI
    11. synaptic vesicle Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 525525Vesicular inhibitory amino acid transporterPRO_0000093822Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei186 – 1861Nitrated tyrosine1 Publication
    Glycosylationi341 – 3411N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein, Nitration

    Proteomic databases

    MaxQBiO35633.
    PaxDbiO35633.
    PRIDEiO35633.

    PTM databases

    PhosphoSiteiO35633.

    Expressioni

    Tissue specificityi

    Brain and retina. Localized in horizontal cell tips at both rod and cone terminals.1 Publication

    Gene expression databases

    BgeeiO35633.
    GenevestigatoriO35633.

    Structurei

    3D structure databases

    ProteinModelPortaliO35633.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 133133CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini155 – 20450Lumenal, vesicleSequence AnalysisAdd
    BLAST
    Topological domaini226 – 24217CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini264 – 2652Lumenal, vesicleSequence Analysis
    Topological domaini287 – 30519CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini327 – 34115Lumenal, vesicleSequence AnalysisAdd
    BLAST
    Topological domaini363 – 38422CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini406 – 43833Lumenal, vesicleSequence AnalysisAdd
    BLAST
    Topological domaini460 – 4612CytoplasmicSequence Analysis
    Topological domaini483 – 4897Lumenal, vesicleSequence Analysis
    Topological domaini511 – 52515CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei134 – 15421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei205 – 22521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei243 – 26321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei266 – 28621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei306 – 32621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei342 – 36221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei385 – 40521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei439 – 45921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei462 – 48221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei490 – 51021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG294245.
    HOGENOMiHOG000220837.
    HOVERGENiHBG061364.
    InParanoidiO35633.
    KOiK15015.
    OMAiMYNSFPN.
    OrthoDBiEOG7SR4M2.
    PhylomeDBiO35633.
    TreeFamiTF312818.

    Family and domain databases

    InterProiIPR013057. AA_transpt_TM.
    [Graphical view]
    PfamiPF01490. Aa_trans. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O35633-1) [UniParc]FASTAAdd to Basket

    Also known as: b

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MATLLRSKLT NVATSVSNKS QAKVSGMFAR MGFQAATDEE AVGFAHCDDL    50
    DFEHRQGLQM DILKSEGEPC GDEGAEAPVE GDIHYQRGGA PLPPSGSKDQ 100
    AVGAGGEFGG HDKPKITAWE AGWNVTNAIQ GMFVLGLPYA ILHGGYLGLF 150
    LIIFAAVVCC YTGKILIACL YEENEDGEVV RVRDSYVAIA NACCAPRFPT 200
    LGGRVVNVAQ IIELVMTCIL YVVVSGNLMY NSFPGLPVSQ KSWSIIATAV 250
    LLPCAFLKNL KAVSKFSLLC TLAHFVINIL VIAYCLSRAR DWAWEKVKFY 300
    IDVKKFPISI GIIVFSYTSQ IFLPSLEGNM QQPSEFHCMM NWTHIAACVL 350
    KGLFALVAYL TWADETKEVI TDNLPGSIRA VVNLFLVAKA LLSYPLPFFA 400
    AVEVLEKSLF QEGSRAFFPA CYGGDGRLKS WGLTLRCALV VFTLLMAIYV 450
    PHFALLMGLT GSLTGAGLCF LLPSLFHLRL LWRKLLWHQV FFDVAIFVIG 500
    GICSVSGFVH SLEGLIEAYR TNAED 525
    Length:525
    Mass (Da):57,381
    Last modified:March 28, 2003 - v3
    Checksum:iEBD63E01A4B54C07
    GO
    Isoform 2 (identifier: O35633-2) [UniParc]FASTAAdd to Basket

    Also known as: a

    The sequence of this isoform differs from the canonical sequence as follows:
         515-525: LIEAYRTNAED → KFAGLET

    Show »
    Length:521
    Mass (Da):56,852
    Checksum:iD4AA407244DD3C7B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti432 – 4321G → E in CAA04864. (PubMed:9395291)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei515 – 52511LIEAYRTNAED → KFAGLET in isoform 2. 1 PublicationVSP_007063Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB080232 Genomic DNA. Translation: BAC44888.1.
    AB080232 Genomic DNA. Translation: BAC44889.1.
    AJ001598 mRNA. Translation: CAA04864.1.
    BC052020 mRNA. Translation: AAH52020.1.
    CCDSiCCDS38309.1. [O35633-1]
    RefSeqiNP_033534.2. NM_009508.2. [O35633-1]
    UniGeneiMm.143404.
    Mm.413854.

    Genome annotation databases

    EnsembliENSMUST00000045738; ENSMUSP00000036299; ENSMUSG00000037771. [O35633-1]
    GeneIDi22348.
    KEGGimmu:22348.
    UCSCiuc008nqk.1. mouse. [O35633-1]
    uc029uhu.1. mouse. [O35633-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB080232 Genomic DNA. Translation: BAC44888.1 .
    AB080232 Genomic DNA. Translation: BAC44889.1 .
    AJ001598 mRNA. Translation: CAA04864.1 .
    BC052020 mRNA. Translation: AAH52020.1 .
    CCDSi CCDS38309.1. [O35633-1 ]
    RefSeqi NP_033534.2. NM_009508.2. [O35633-1 ]
    UniGenei Mm.143404.
    Mm.413854.

    3D structure databases

    ProteinModelPortali O35633.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    TCDBi 2.A.18.5.3. the amino acid/auxin permease (aaap) family.

    PTM databases

    PhosphoSitei O35633.

    Proteomic databases

    MaxQBi O35633.
    PaxDbi O35633.
    PRIDEi O35633.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000045738 ; ENSMUSP00000036299 ; ENSMUSG00000037771 . [O35633-1 ]
    GeneIDi 22348.
    KEGGi mmu:22348.
    UCSCi uc008nqk.1. mouse. [O35633-1 ]
    uc029uhu.1. mouse. [O35633-2 ]

    Organism-specific databases

    CTDi 140679.
    MGIi MGI:1194488. Slc32a1.

    Phylogenomic databases

    eggNOGi NOG294245.
    HOGENOMi HOG000220837.
    HOVERGENi HBG061364.
    InParanoidi O35633.
    KOi K15015.
    OMAi MYNSFPN.
    OrthoDBi EOG7SR4M2.
    PhylomeDBi O35633.
    TreeFami TF312818.

    Miscellaneous databases

    NextBioi 302627.
    PROi O35633.
    SOURCEi Search...

    Gene expression databases

    Bgeei O35633.
    Genevestigatori O35633.

    Family and domain databases

    InterProi IPR013057. AA_transpt_TM.
    [Graphical view ]
    Pfami PF01490. Aa_trans. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of a functional vesicular GABA and glycine transporter by screening of genome databases."
      Sagne C., El Mestikawy S., Isambert M.-F., Hamon M., Henry J.-P., Giros B.P., Gasnier B.
      FEBS Lett. 417:177-183(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Strain: BALB/c.
      Tissue: Brain.
    2. "Mouse vesicular GABA transporter gene: genomic organization, transcriptional regulation and chromosomal localization."
      Ebihara S., Obata K., Yanagawa Y.
      Brain Res. Mol. Brain Res. 110:126-139(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2).
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6.
      Tissue: Brain.
    4. "Cellular localization of the vesicular inhibitory amino acid transporter in the mouse and human retina."
      Jellali A., Stussi-Garaud C., Gasnier B., Rendon A., Sahel J.-A., Dreyfus H., Picaud S.
      J. Comp. Neurol. 449:76-87(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    5. Cited for: NITRATION [LARGE SCALE ANALYSIS] AT TYR-186, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.

    Entry informationi

    Entry nameiVIAAT_MOUSE
    AccessioniPrimary (citable) accession number: O35633
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 28, 2003
    Last sequence update: March 28, 2003
    Last modified: October 1, 2014
    This is version 123 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3