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O35633

- VIAAT_MOUSE

UniProt

O35633 - VIAAT_MOUSE

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Protein

Vesicular inhibitory amino acid transporter

Gene

Slc32a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the uptake of GABA and glycine into the synaptic vesicles.

GO - Molecular functioni

  1. gamma-aminobutyric acid:proton symporter activity Source: MGI
  2. glycine transmembrane transporter activity Source: MGI

GO - Biological processi

  1. aging Source: Ensembl
  2. gamma-aminobutyric acid transport Source: GOC
  3. glycine transport Source: MGI
  4. neurotransmitter transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Transport

Protein family/group databases

TCDBi2.A.18.5.3. the amino acid/auxin permease (aaap) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicular inhibitory amino acid transporter
Alternative name(s):
GABA and glycine transporter
Solute carrier family 32 member 1
Vesicular GABA transporter
Short name:
mVGAT
Short name:
mVIAAT
Gene namesi
Name:Slc32a1
Synonyms:Vgat, Viaat
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:1194488. Slc32a1.

Subcellular locationi

GO - Cellular componenti

  1. cell tip Source: MGI
  2. cone cell pedicle Source: MGI
  3. dendrite Source: MGI
  4. dendrite terminus Source: MGI
  5. inhibitory synapse Source: MGI
  6. integral component of plasma membrane Source: MGI
  7. neuron projection Source: MGI
  8. neuron projection terminus Source: MGI
  9. synapse Source: MGI
  10. synaptic vesicle Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 525525Vesicular inhibitory amino acid transporterPRO_0000093822Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei186 – 1861Nitrated tyrosine1 Publication
Glycosylationi341 – 3411N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein, Nitration

Proteomic databases

MaxQBiO35633.
PaxDbiO35633.
PRIDEiO35633.

PTM databases

PhosphoSiteiO35633.

Expressioni

Tissue specificityi

Brain and retina. Localized in horizontal cell tips at both rod and cone terminals.1 Publication

Gene expression databases

BgeeiO35633.
ExpressionAtlasiO35633. baseline and differential.
GenevestigatoriO35633.

Structurei

3D structure databases

ProteinModelPortaliO35633.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 133133CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini155 – 20450Lumenal, vesicleSequence AnalysisAdd
BLAST
Topological domaini226 – 24217CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini264 – 2652Lumenal, vesicleSequence Analysis
Topological domaini287 – 30519CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini327 – 34115Lumenal, vesicleSequence AnalysisAdd
BLAST
Topological domaini363 – 38422CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini406 – 43833Lumenal, vesicleSequence AnalysisAdd
BLAST
Topological domaini460 – 4612CytoplasmicSequence Analysis
Topological domaini483 – 4897Lumenal, vesicleSequence Analysis
Topological domaini511 – 52515CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei134 – 15421HelicalSequence AnalysisAdd
BLAST
Transmembranei205 – 22521HelicalSequence AnalysisAdd
BLAST
Transmembranei243 – 26321HelicalSequence AnalysisAdd
BLAST
Transmembranei266 – 28621HelicalSequence AnalysisAdd
BLAST
Transmembranei306 – 32621HelicalSequence AnalysisAdd
BLAST
Transmembranei342 – 36221HelicalSequence AnalysisAdd
BLAST
Transmembranei385 – 40521HelicalSequence AnalysisAdd
BLAST
Transmembranei439 – 45921HelicalSequence AnalysisAdd
BLAST
Transmembranei462 – 48221HelicalSequence AnalysisAdd
BLAST
Transmembranei490 – 51021HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG294245.
HOGENOMiHOG000220837.
HOVERGENiHBG061364.
InParanoidiO35633.
KOiK15015.
OMAiMYNSFPN.
OrthoDBiEOG7SR4M2.
PhylomeDBiO35633.
TreeFamiTF312818.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O35633-1) [UniParc]FASTAAdd to Basket

Also known as: b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATLLRSKLT NVATSVSNKS QAKVSGMFAR MGFQAATDEE AVGFAHCDDL
60 70 80 90 100
DFEHRQGLQM DILKSEGEPC GDEGAEAPVE GDIHYQRGGA PLPPSGSKDQ
110 120 130 140 150
AVGAGGEFGG HDKPKITAWE AGWNVTNAIQ GMFVLGLPYA ILHGGYLGLF
160 170 180 190 200
LIIFAAVVCC YTGKILIACL YEENEDGEVV RVRDSYVAIA NACCAPRFPT
210 220 230 240 250
LGGRVVNVAQ IIELVMTCIL YVVVSGNLMY NSFPGLPVSQ KSWSIIATAV
260 270 280 290 300
LLPCAFLKNL KAVSKFSLLC TLAHFVINIL VIAYCLSRAR DWAWEKVKFY
310 320 330 340 350
IDVKKFPISI GIIVFSYTSQ IFLPSLEGNM QQPSEFHCMM NWTHIAACVL
360 370 380 390 400
KGLFALVAYL TWADETKEVI TDNLPGSIRA VVNLFLVAKA LLSYPLPFFA
410 420 430 440 450
AVEVLEKSLF QEGSRAFFPA CYGGDGRLKS WGLTLRCALV VFTLLMAIYV
460 470 480 490 500
PHFALLMGLT GSLTGAGLCF LLPSLFHLRL LWRKLLWHQV FFDVAIFVIG
510 520
GICSVSGFVH SLEGLIEAYR TNAED
Length:525
Mass (Da):57,381
Last modified:March 28, 2003 - v3
Checksum:iEBD63E01A4B54C07
GO
Isoform 2 (identifier: O35633-2) [UniParc]FASTAAdd to Basket

Also known as: a

The sequence of this isoform differs from the canonical sequence as follows:
     515-525: LIEAYRTNAED → KFAGLET

Show »
Length:521
Mass (Da):56,852
Checksum:iD4AA407244DD3C7B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti432 – 4321G → E in CAA04864. (PubMed:9395291)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei515 – 52511LIEAYRTNAED → KFAGLET in isoform 2. 1 PublicationVSP_007063Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB080232 Genomic DNA. Translation: BAC44888.1.
AB080232 Genomic DNA. Translation: BAC44889.1.
AJ001598 mRNA. Translation: CAA04864.1.
BC052020 mRNA. Translation: AAH52020.1.
CCDSiCCDS38309.1. [O35633-1]
RefSeqiNP_033534.2. NM_009508.2. [O35633-1]
UniGeneiMm.143404.
Mm.413854.

Genome annotation databases

EnsembliENSMUST00000045738; ENSMUSP00000036299; ENSMUSG00000037771. [O35633-1]
GeneIDi22348.
KEGGimmu:22348.
UCSCiuc008nqk.1. mouse. [O35633-1]
uc029uhu.1. mouse. [O35633-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB080232 Genomic DNA. Translation: BAC44888.1 .
AB080232 Genomic DNA. Translation: BAC44889.1 .
AJ001598 mRNA. Translation: CAA04864.1 .
BC052020 mRNA. Translation: AAH52020.1 .
CCDSi CCDS38309.1. [O35633-1 ]
RefSeqi NP_033534.2. NM_009508.2. [O35633-1 ]
UniGenei Mm.143404.
Mm.413854.

3D structure databases

ProteinModelPortali O35633.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

TCDBi 2.A.18.5.3. the amino acid/auxin permease (aaap) family.

PTM databases

PhosphoSitei O35633.

Proteomic databases

MaxQBi O35633.
PaxDbi O35633.
PRIDEi O35633.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000045738 ; ENSMUSP00000036299 ; ENSMUSG00000037771 . [O35633-1 ]
GeneIDi 22348.
KEGGi mmu:22348.
UCSCi uc008nqk.1. mouse. [O35633-1 ]
uc029uhu.1. mouse. [O35633-2 ]

Organism-specific databases

CTDi 140679.
MGIi MGI:1194488. Slc32a1.

Phylogenomic databases

eggNOGi NOG294245.
HOGENOMi HOG000220837.
HOVERGENi HBG061364.
InParanoidi O35633.
KOi K15015.
OMAi MYNSFPN.
OrthoDBi EOG7SR4M2.
PhylomeDBi O35633.
TreeFami TF312818.

Miscellaneous databases

NextBioi 302627.
PROi O35633.
SOURCEi Search...

Gene expression databases

Bgeei O35633.
ExpressionAtlasi O35633. baseline and differential.
Genevestigatori O35633.

Family and domain databases

InterProi IPR013057. AA_transpt_TM.
[Graphical view ]
Pfami PF01490. Aa_trans. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of a functional vesicular GABA and glycine transporter by screening of genome databases."
    Sagne C., El Mestikawy S., Isambert M.-F., Hamon M., Henry J.-P., Giros B.P., Gasnier B.
    FEBS Lett. 417:177-183(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: BALB/c.
    Tissue: Brain.
  2. "Mouse vesicular GABA transporter gene: genomic organization, transcriptional regulation and chromosomal localization."
    Ebihara S., Obata K., Yanagawa Y.
    Brain Res. Mol. Brain Res. 110:126-139(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2).
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6.
    Tissue: Brain.
  4. "Cellular localization of the vesicular inhibitory amino acid transporter in the mouse and human retina."
    Jellali A., Stussi-Garaud C., Gasnier B., Rendon A., Sahel J.-A., Dreyfus H., Picaud S.
    J. Comp. Neurol. 449:76-87(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. Cited for: NITRATION [LARGE SCALE ANALYSIS] AT TYR-186, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiVIAAT_MOUSE
AccessioniPrimary (citable) accession number: O35633
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: March 28, 2003
Last modified: October 29, 2014
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3