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O35633 (VIAAT_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vesicular inhibitory amino acid transporter
Alternative name(s):
GABA and glycine transporter
Solute carrier family 32 member 1
Vesicular GABA transporter
Short name=mVGAT
Short name=mVIAAT
Gene names
Name:Slc32a1
Synonyms:Vgat, Viaat
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length525 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the uptake of GABA and glycine into the synaptic vesicles.

Subcellular location

Cytoplasmic vesicle membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Brain and retina. Localized in horizontal cell tips at both rod and cone terminals. Ref.4

Sequence similarities

Belongs to the amino acid/polyamine transporter 2 family.

Ontologies

Keywords
   Biological processNeurotransmitter transport
Transport
   Cellular componentCytoplasmic vesicle
Membrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   PTMGlycoprotein
Nitration
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaging

Inferred from electronic annotation. Source: Ensembl

gamma-aminobutyric acid transport

Inferred from direct assay Ref.1. Source: GOC

glycine transport

Inferred from direct assay Ref.1. Source: MGI

neurotransmitter transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcell tip

Inferred from direct assay Ref.4. Source: MGI

cone cell pedicle

Inferred from direct assay Ref.4. Source: MGI

cytoplasmic vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

dendrite

Inferred from sequence orthology Ref.4. Source: MGI

dendrite terminus

Inferred from direct assay Ref.4. Source: MGI

inhibitory synapse

Inferred from direct assay PubMed 16814779PubMed 17823315PubMed 21356198. Source: MGI

integral component of plasma membrane

Inferred by curator Ref.1. Source: MGI

neuron projection

Inferred from direct assay Ref.4. Source: MGI

neuron projection terminus

Inferred from direct assay Ref.4. Source: MGI

synapse

Inferred from sequence orthology PubMed 15681343. Source: MGI

synaptic vesicle

Inferred from electronic annotation. Source: Ensembl

   Molecular_functiongamma-aminobutyric acid:proton symporter activity

Inferred from direct assay Ref.1. Source: MGI

glycine transmembrane transporter activity

Inferred from direct assay Ref.1. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O35633-1)

Also known as: b;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O35633-2)

Also known as: a;

The sequence of this isoform differs from the canonical sequence as follows:
     515-525: LIEAYRTNAED → KFAGLET

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 525525Vesicular inhibitory amino acid transporter
PRO_0000093822

Regions

Topological domain1 – 133133Cytoplasmic Potential
Transmembrane134 – 15421Helical; Potential
Topological domain155 – 20450Lumenal, vesicle Potential
Transmembrane205 – 22521Helical; Potential
Topological domain226 – 24217Cytoplasmic Potential
Transmembrane243 – 26321Helical; Potential
Topological domain264 – 2652Lumenal, vesicle Potential
Transmembrane266 – 28621Helical; Potential
Topological domain287 – 30519Cytoplasmic Potential
Transmembrane306 – 32621Helical; Potential
Topological domain327 – 34115Lumenal, vesicle Potential
Transmembrane342 – 36221Helical; Potential
Topological domain363 – 38422Cytoplasmic Potential
Transmembrane385 – 40521Helical; Potential
Topological domain406 – 43833Lumenal, vesicle Potential
Transmembrane439 – 45921Helical; Potential
Topological domain460 – 4612Cytoplasmic Potential
Transmembrane462 – 48221Helical; Potential
Topological domain483 – 4897Lumenal, vesicle Potential
Transmembrane490 – 51021Helical; Potential
Topological domain511 – 52515Cytoplasmic Potential

Amino acid modifications

Modified residue1861Nitrated tyrosine Ref.5
Glycosylation3411N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence515 – 52511LIEAYRTNAED → KFAGLET in isoform 2.
VSP_007063

Experimental info

Sequence conflict4321G → E in CAA04864. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (b) [UniParc].

Last modified March 28, 2003. Version 3.
Checksum: EBD63E01A4B54C07

FASTA52557,381
        10         20         30         40         50         60 
MATLLRSKLT NVATSVSNKS QAKVSGMFAR MGFQAATDEE AVGFAHCDDL DFEHRQGLQM 

        70         80         90        100        110        120 
DILKSEGEPC GDEGAEAPVE GDIHYQRGGA PLPPSGSKDQ AVGAGGEFGG HDKPKITAWE 

       130        140        150        160        170        180 
AGWNVTNAIQ GMFVLGLPYA ILHGGYLGLF LIIFAAVVCC YTGKILIACL YEENEDGEVV 

       190        200        210        220        230        240 
RVRDSYVAIA NACCAPRFPT LGGRVVNVAQ IIELVMTCIL YVVVSGNLMY NSFPGLPVSQ 

       250        260        270        280        290        300 
KSWSIIATAV LLPCAFLKNL KAVSKFSLLC TLAHFVINIL VIAYCLSRAR DWAWEKVKFY 

       310        320        330        340        350        360 
IDVKKFPISI GIIVFSYTSQ IFLPSLEGNM QQPSEFHCMM NWTHIAACVL KGLFALVAYL 

       370        380        390        400        410        420 
TWADETKEVI TDNLPGSIRA VVNLFLVAKA LLSYPLPFFA AVEVLEKSLF QEGSRAFFPA 

       430        440        450        460        470        480 
CYGGDGRLKS WGLTLRCALV VFTLLMAIYV PHFALLMGLT GSLTGAGLCF LLPSLFHLRL 

       490        500        510        520 
LWRKLLWHQV FFDVAIFVIG GICSVSGFVH SLEGLIEAYR TNAED 

« Hide

Isoform 2 (a) [UniParc].

Checksum: D4AA407244DD3C7B
Show »

FASTA52156,852

References

« Hide 'large scale' references
[1]"Cloning of a functional vesicular GABA and glycine transporter by screening of genome databases."
Sagne C., El Mestikawy S., Isambert M.-F., Hamon M., Henry J.-P., Giros B.P., Gasnier B.
FEBS Lett. 417:177-183(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: BALB/c.
Tissue: Brain.
[2]"Mouse vesicular GABA transporter gene: genomic organization, transcriptional regulation and chromosomal localization."
Ebihara S., Obata K., Yanagawa Y.
Brain Res. Mol. Brain Res. 110:126-139(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2).
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6.
Tissue: Brain.
[4]"Cellular localization of the vesicular inhibitory amino acid transporter in the mouse and human retina."
Jellali A., Stussi-Garaud C., Gasnier B., Rendon A., Sahel J.-A., Dreyfus H., Picaud S.
J. Comp. Neurol. 449:76-87(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[5]"Endogenously nitrated proteins in mouse brain: links to neurodegenerative disease."
Sacksteder C.A., Qian W.-J., Knyushko T.V., Wang H., Chin M.H., Lacan G., Melega W.P., Camp D.G. II, Smith R.D., Smith D.J., Squier T.C., Bigelow D.J.
Biochemistry 45:8009-8022(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NITRATION [LARGE SCALE ANALYSIS] AT TYR-186, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB080232 Genomic DNA. Translation: BAC44888.1.
AB080232 Genomic DNA. Translation: BAC44889.1.
AJ001598 mRNA. Translation: CAA04864.1.
BC052020 mRNA. Translation: AAH52020.1.
CCDSCCDS38309.1. [O35633-1]
RefSeqNP_033534.2. NM_009508.2. [O35633-1]
UniGeneMm.143404.
Mm.413854.

3D structure databases

ProteinModelPortalO35633.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

TCDB2.A.18.5.3. the amino acid/auxin permease (aaap) family.

PTM databases

PhosphoSiteO35633.

Proteomic databases

MaxQBO35633.
PaxDbO35633.
PRIDEO35633.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000045738; ENSMUSP00000036299; ENSMUSG00000037771. [O35633-1]
GeneID22348.
KEGGmmu:22348.
UCSCuc008nqk.1. mouse. [O35633-1]
uc029uhu.1. mouse. [O35633-2]

Organism-specific databases

CTD140679.
MGIMGI:1194488. Slc32a1.

Phylogenomic databases

eggNOGNOG294245.
HOGENOMHOG000220837.
HOVERGENHBG061364.
InParanoidO35633.
KOK15015.
OMAMYNSFPN.
OrthoDBEOG7SR4M2.
PhylomeDBO35633.
TreeFamTF312818.

Gene expression databases

BgeeO35633.
GenevestigatorO35633.

Family and domain databases

InterProIPR013057. AA_transpt_TM.
[Graphical view]
PfamPF01490. Aa_trans. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio302627.
PROO35633.
SOURCESearch...

Entry information

Entry nameVIAAT_MOUSE
AccessionPrimary (citable) accession number: O35633
Entry history
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: March 28, 2003
Last modified: July 9, 2014
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot