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Protein

Vesicular inhibitory amino acid transporter

Gene

Slc32a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the uptake of GABA and glycine into the synaptic vesicles.By similarity1 Publication

GO - Molecular functioni

  • gamma-aminobutyric acid:proton symporter activity Source: MGI
  • glycine transmembrane transporter activity Source: MGI

GO - Biological processi

  • aging Source: Ensembl
  • glycine transport Source: MGI
  • hippocampus development Source: Ensembl
  • neurotransmitter transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-425393. Transport of inorganic cations/anions and amino acids/oligopeptides.
R-MMU-888590. GABA synthesis, release, reuptake and degradation.

Protein family/group databases

TCDBi2.A.18.5.3. the amino acid/auxin permease (aaap) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicular inhibitory amino acid transporter
Alternative name(s):
GABA and glycine transporter
Solute carrier family 32 member 1
Vesicular GABA transporter
Short name:
mVGAT
Short name:
mVIAAT
Gene namesi
Name:Slc32a1
Synonyms:Vgat, Viaat
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1194488. Slc32a1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 133CytoplasmicSequence analysisAdd BLAST133
Transmembranei134 – 154HelicalSequence analysisAdd BLAST21
Topological domaini155 – 204Lumenal, vesicleSequence analysisAdd BLAST50
Transmembranei205 – 225HelicalSequence analysisAdd BLAST21
Topological domaini226 – 242CytoplasmicSequence analysisAdd BLAST17
Transmembranei243 – 263HelicalSequence analysisAdd BLAST21
Topological domaini264 – 265Lumenal, vesicleSequence analysis2
Transmembranei266 – 286HelicalSequence analysisAdd BLAST21
Topological domaini287 – 305CytoplasmicSequence analysisAdd BLAST19
Transmembranei306 – 326HelicalSequence analysisAdd BLAST21
Topological domaini327 – 341Lumenal, vesicleSequence analysisAdd BLAST15
Transmembranei342 – 362HelicalSequence analysisAdd BLAST21
Topological domaini363 – 384CytoplasmicSequence analysisAdd BLAST22
Transmembranei385 – 405HelicalSequence analysisAdd BLAST21
Topological domaini406 – 438Lumenal, vesicleSequence analysisAdd BLAST33
Transmembranei439 – 459HelicalSequence analysisAdd BLAST21
Topological domaini460 – 461CytoplasmicSequence analysis2
Transmembranei462 – 482HelicalSequence analysisAdd BLAST21
Topological domaini483 – 489Lumenal, vesicleSequence analysis7
Transmembranei490 – 510HelicalSequence analysisAdd BLAST21
Topological domaini511 – 525CytoplasmicSequence analysisAdd BLAST15

GO - Cellular componenti

  • cell tip Source: MGI
  • cone cell pedicle Source: MGI
  • cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  • dendrite Source: MGI
  • dendrite terminus Source: MGI
  • inhibitory synapse Source: MGI
  • integral component of plasma membrane Source: MGI
  • neuron projection Source: MGI
  • neuron projection terminus Source: MGI
  • presynaptic active zone Source: MGI
  • synapse Source: MGI
  • synaptic vesicle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Disruption phenotypei

Deficient mice exhibit embryonic lethality and a cleft palate and omphalocele.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000938221 – 525Vesicular inhibitory amino acid transporterAdd BLAST525

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei186Nitrated tyrosineCombined sources1
Glycosylationi341N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein, Nitration

Proteomic databases

PaxDbiO35633.
PeptideAtlasiO35633.
PRIDEiO35633.

PTM databases

iPTMnetiO35633.
PhosphoSitePlusiO35633.
SwissPalmiO35633.

Expressioni

Tissue specificityi

Brain and retina. Localized in horizontal cell tips at both rod and cone terminals.1 Publication

Gene expression databases

BgeeiENSMUSG00000037771.
ExpressionAtlasiO35633. baseline and differential.
GenevisibleiO35633. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036299.

Structurei

3D structure databases

ProteinModelPortaliO35633.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4303. Eukaryota.
COG0814. LUCA.
GeneTreeiENSGT00490000043380.
HOGENOMiHOG000220837.
HOVERGENiHBG061364.
InParanoidiO35633.
KOiK15015.
OMAiANACCSP.
OrthoDBiEOG091G05MY.
PhylomeDBiO35633.
TreeFamiTF312818.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O35633-1) [UniParc]FASTAAdd to basket
Also known as: b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATLLRSKLT NVATSVSNKS QAKVSGMFAR MGFQAATDEE AVGFAHCDDL
60 70 80 90 100
DFEHRQGLQM DILKSEGEPC GDEGAEAPVE GDIHYQRGGA PLPPSGSKDQ
110 120 130 140 150
AVGAGGEFGG HDKPKITAWE AGWNVTNAIQ GMFVLGLPYA ILHGGYLGLF
160 170 180 190 200
LIIFAAVVCC YTGKILIACL YEENEDGEVV RVRDSYVAIA NACCAPRFPT
210 220 230 240 250
LGGRVVNVAQ IIELVMTCIL YVVVSGNLMY NSFPGLPVSQ KSWSIIATAV
260 270 280 290 300
LLPCAFLKNL KAVSKFSLLC TLAHFVINIL VIAYCLSRAR DWAWEKVKFY
310 320 330 340 350
IDVKKFPISI GIIVFSYTSQ IFLPSLEGNM QQPSEFHCMM NWTHIAACVL
360 370 380 390 400
KGLFALVAYL TWADETKEVI TDNLPGSIRA VVNLFLVAKA LLSYPLPFFA
410 420 430 440 450
AVEVLEKSLF QEGSRAFFPA CYGGDGRLKS WGLTLRCALV VFTLLMAIYV
460 470 480 490 500
PHFALLMGLT GSLTGAGLCF LLPSLFHLRL LWRKLLWHQV FFDVAIFVIG
510 520
GICSVSGFVH SLEGLIEAYR TNAED
Length:525
Mass (Da):57,381
Last modified:March 28, 2003 - v3
Checksum:iEBD63E01A4B54C07
GO
Isoform 2 (identifier: O35633-2) [UniParc]FASTAAdd to basket
Also known as: a

The sequence of this isoform differs from the canonical sequence as follows:
     515-525: LIEAYRTNAED → KFAGLET

Show »
Length:521
Mass (Da):56,852
Checksum:iD4AA407244DD3C7B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti432G → E in CAA04864 (PubMed:9395291).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007063515 – 525LIEAYRTNAED → KFAGLET in isoform 2. 1 PublicationAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB080232 Genomic DNA. Translation: BAC44888.1.
AB080232 Genomic DNA. Translation: BAC44889.1.
AJ001598 mRNA. Translation: CAA04864.1.
BC052020 mRNA. Translation: AAH52020.1.
CCDSiCCDS38309.1. [O35633-1]
RefSeqiNP_033534.2. NM_009508.2. [O35633-1]
UniGeneiMm.143404.
Mm.413854.

Genome annotation databases

EnsembliENSMUST00000045738; ENSMUSP00000036299; ENSMUSG00000037771. [O35633-1]
GeneIDi22348.
KEGGimmu:22348.
UCSCiuc008nqk.1. mouse. [O35633-1]
uc029uhu.1. mouse. [O35633-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB080232 Genomic DNA. Translation: BAC44888.1.
AB080232 Genomic DNA. Translation: BAC44889.1.
AJ001598 mRNA. Translation: CAA04864.1.
BC052020 mRNA. Translation: AAH52020.1.
CCDSiCCDS38309.1. [O35633-1]
RefSeqiNP_033534.2. NM_009508.2. [O35633-1]
UniGeneiMm.143404.
Mm.413854.

3D structure databases

ProteinModelPortaliO35633.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036299.

Protein family/group databases

TCDBi2.A.18.5.3. the amino acid/auxin permease (aaap) family.

PTM databases

iPTMnetiO35633.
PhosphoSitePlusiO35633.
SwissPalmiO35633.

Proteomic databases

PaxDbiO35633.
PeptideAtlasiO35633.
PRIDEiO35633.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045738; ENSMUSP00000036299; ENSMUSG00000037771. [O35633-1]
GeneIDi22348.
KEGGimmu:22348.
UCSCiuc008nqk.1. mouse. [O35633-1]
uc029uhu.1. mouse. [O35633-2]

Organism-specific databases

CTDi140679.
MGIiMGI:1194488. Slc32a1.

Phylogenomic databases

eggNOGiKOG4303. Eukaryota.
COG0814. LUCA.
GeneTreeiENSGT00490000043380.
HOGENOMiHOG000220837.
HOVERGENiHBG061364.
InParanoidiO35633.
KOiK15015.
OMAiANACCSP.
OrthoDBiEOG091G05MY.
PhylomeDBiO35633.
TreeFamiTF312818.

Enzyme and pathway databases

ReactomeiR-MMU-425393. Transport of inorganic cations/anions and amino acids/oligopeptides.
R-MMU-888590. GABA synthesis, release, reuptake and degradation.

Miscellaneous databases

PROiO35633.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037771.
ExpressionAtlasiO35633. baseline and differential.
GenevisibleiO35633. MM.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVIAAT_MOUSE
AccessioniPrimary (citable) accession number: O35633
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: March 28, 2003
Last modified: November 2, 2016
This is version 140 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.