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O35632

- HYAL2_MOUSE

UniProt

O35632 - HYAL2_MOUSE

Protein

Hyaluronidase-2

Gene

Hyal2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 2 (15 Nov 2002)
      Previous versions | rss
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    Functioni

    Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R By similarity.By similarity

    Catalytic activityi

    Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei135 – 1351Proton donorBy similarity

    GO - Molecular functioni

    1. enzyme binding Source: BHF-UCL
    2. hyaluronic acid binding Source: UniProtKB
    3. hyaluronoglucuronidase activity Source: UniProtKB
    4. hyalurononglucosaminidase activity Source: UniProtKB
    5. receptor signaling protein tyrosine kinase inhibitor activity Source: UniProtKB
    6. transforming growth factor beta binding Source: BHF-UCL
    7. virus receptor activity Source: UniProtKB

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. cartilage development Source: UniProtKB
    3. cellular response to fibroblast growth factor stimulus Source: UniProtKB
    4. cellular response to interleukin-1 Source: UniProtKB
    5. cellular response to transforming growth factor beta stimulus Source: BHF-UCL
    6. cellular response to tumor necrosis factor Source: Ensembl
    7. cellular response to UV-B Source: UniProtKB
    8. defense response to virus Source: UniProtKB
    9. fusion of virus membrane with host plasma membrane Source: UniProtKB
    10. glycosaminoglycan catabolic process Source: UniProtKB
    11. hematopoietic progenitor cell differentiation Source: MGI
    12. hyaluronan catabolic process Source: UniProtKB
    13. monocyte activation Source: UniProtKB
    14. multicellular organismal aging Source: MGI
    15. multicellular organismal iron ion homeostasis Source: MGI
    16. negative regulation of cell growth Source: UniProtKB
    17. negative regulation of fibroblast migration Source: UniProtKB
    18. negative regulation of MAP kinase activity Source: UniProtKB
    19. negative regulation of protein kinase B signaling Source: UniProtKB
    20. negative regulation of protein tyrosine kinase activity Source: UniProtKB
    21. positive regulation of extrinsic apoptotic signaling pathway Source: BHF-UCL
    22. positive regulation of inflammatory response Source: UniProtKB
    23. positive regulation of interleukin-6 secretion Source: UniProtKB
    24. positive regulation of interleukin-8 secretion Source: UniProtKB
    25. positive regulation of protein import into nucleus Source: BHF-UCL
    26. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
    27. positive regulation of urine volume Source: UniProtKB
    28. renal water absorption Source: UniProtKB
    29. response to antibiotic Source: Ensembl
    30. response to reactive oxygen species Source: UniProtKB
    31. response to virus Source: UniProtKB
    32. skeletal system morphogenesis Source: MGI
    33. transformation of host cell by virus Source: UniProtKB
    34. viral entry into host cell Source: UniProtKB

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Receptor

    Enzyme and pathway databases

    ReactomeiREACT_196554. Hyaluronan uptake and degradation.

    Protein family/group databases

    CAZyiGH56. Glycoside Hydrolase Family 56.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hyaluronidase-2 (EC:3.2.1.35)
    Short name:
    Hyal-2
    Alternative name(s):
    Hyaluronoglucosaminidase-2
    Gene namesi
    Name:Hyal2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:1196334. Hyal2.

    Subcellular locationi

    Cell membrane By similarity; Lipid-anchorGPI-anchor By similarity

    GO - Cellular componenti

    1. anchored component of plasma membrane Source: UniProtKB
    2. apical plasma membrane Source: UniProtKB
    3. cell surface Source: BHF-UCL
    4. cytoplasmic membrane-bounded vesicle Source: UniProtKB
    5. cytoplasmic vesicle Source: UniProtKB
    6. cytosol Source: UniProtKB
    7. endocytic vesicle Source: UniProtKB
    8. Golgi membrane Source: UniProtKB
    9. lysosome Source: UniProtKB
    10. membrane raft Source: UniProtKB
    11. microvillus Source: UniProtKB
    12. perinuclear region of cytoplasm Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – 448428Hyaluronidase-2PRO_0000012101Add
    BLAST
    Propeptidei449 – 47325Removed in mature formSequence AnalysisPRO_0000012102Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi47 ↔ 340By similarity
    Glycosylationi74 – 741N-linked (GlcNAc...)1 Publication
    Glycosylationi103 – 1031N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi211 ↔ 227By similarity
    Glycosylationi357 – 3571N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi365 ↔ 376By similarity
    Disulfide bondi370 ↔ 427By similarity
    Glycosylationi390 – 3901N-linked (GlcNAc...)1 Publication
    Disulfide bondi429 ↔ 438By similarity
    Lipidationi448 – 4481GPI-anchor amidated asparagine; alternateSequence Analysis
    Glycosylationi448 – 4481N-linked (GlcNAc...); alternateSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    MaxQBiO35632.
    PRIDEiO35632.

    Expressioni

    Tissue specificityi

    Widely expressed. In the brain, expressed during embryonic stages but expression decreases after birth and is barely detectable in adult brain.1 Publication

    Gene expression databases

    ArrayExpressiO35632.
    BgeeiO35632.
    CleanExiMM_HYAL2.
    GenevestigatoriO35632.

    Interactioni

    Subunit structurei

    Interacts with MST1R.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO35632.
    SMRiO35632. Positions 29-440.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini361 – 43979EGF-likeAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 56 family.Curated
    Contains 1 EGF-like domain.Curated

    Keywords - Domaini

    EGF-like domain, Signal

    Phylogenomic databases

    eggNOGiNOG77606.
    HOGENOMiHOG000015133.
    HOVERGENiHBG052053.
    InParanoidiO35632.
    KOiK01197.
    OMAiGWGGEQC.
    OrthoDBiEOG74J97S.
    PhylomeDBiO35632.
    TreeFamiTF321598.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR000742. EG-like_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR018155. Hyaluronidase.
    [Graphical view]
    PANTHERiPTHR11769. PTHR11769. 1 hit.
    PfamiPF01630. Glyco_hydro_56. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
    PRINTSiPR00846. GLHYDRLASE56.
    SMARTiSM00181. EGF. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00022. EGF_1. 1 hit.
    PS01186. EGF_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O35632-1 [UniParc]FASTAAdd to Basket

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    MRAGLGPIIT LALVLEVAWA GELKPTAPPI FTGRPFVVAW NVPTQECAPR    50
    HKVPLDLRAF DVKATPNEGF FNQNITTFYY DRLGLYPRFD AAGTSVHGGV 100
    PQNGSLCAHL PMLKESVERY IQTQEPGGLA VIDWEEWRPV WVRNWQEKDV 150
    YRQSSRQLVA SRHPDWPSDR VMKQAQYEFE FAARQFMLNT LRYVKAVRPQ 200
    HLWGFYLFPD CYNHDYVQNW ESYTGRCPDV EVARNDQLAW LWAESTALFP 250
    SVYLDETLAS SVHSRNFVSF RVREALRVAH THHANHALPV YVFTRPTYTR 300
    GLTGLSQVDL ISTIGESAAL GSAGVIFWGD SEDASSMETC QYLKNYLTQL 350
    LVPYIVNVSW ATQYCSWTQC HGHGRCVRRN PSANTFLHLN ASSFRLVPGH 400
    TPSEPQLRPE GQLSEADLNY LQKHFRCQCY LGWGGEQCQR NYKGAAGNAS 450
    RAWAGSHLTS LLGLVAVALT WTL 473
    Length:473
    Mass (Da):53,618
    Last modified:November 15, 2002 - v2
    Checksum:iDD433C9FFCF8147C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti26 – 272TA → KP in CAA03888. (PubMed:9790770)Curated
    Sequence conflicti26 – 272TA → KP in CAA03889. (PubMed:9790770)Curated
    Sequence conflicti193 – 1997YVKAVRP → LRQGSQT in CAA03888. (PubMed:9790770)Curated
    Sequence conflicti250 – 2501Missing in CAA03888. (PubMed:9790770)Curated
    Sequence conflicti269 – 2724SFRV → RFGG in CAA03888. (PubMed:9790770)Curated
    Sequence conflicti355 – 3551I → V in AAK28481. (PubMed:11296287)Curated
    Sequence conflicti383 – 3831A → V in CAA03888. (PubMed:9790770)Curated
    Sequence conflicti383 – 3831A → V in CAA03889. (PubMed:9790770)Curated
    Sequence conflicti416 – 4227ADLNYLQ → RDRQLPE in CAA03888. (PubMed:9790770)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ000059 mRNA. Translation: CAA03888.1.
    AJ000060 Genomic DNA. Translation: CAA03889.1.
    AF302843 mRNA. Translation: AAK28481.1.
    AF302844 mRNA. Translation: AAK28482.1.
    AF422177 mRNA. Translation: AAL17823.1.
    CCDSiCCDS23496.1.
    RefSeqiNP_034619.2. NM_010489.2.
    XP_006511706.1. XM_006511643.1.
    XP_006511707.1. XM_006511644.1.
    XP_006511708.1. XM_006511645.1.
    UniGeneiMm.440982.
    Mm.4834.

    Genome annotation databases

    EnsembliENSMUST00000010191; ENSMUSP00000010191; ENSMUSG00000010047.
    GeneIDi15587.
    KEGGimmu:15587.
    UCSCiuc009rlu.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ000059 mRNA. Translation: CAA03888.1 .
    AJ000060 Genomic DNA. Translation: CAA03889.1 .
    AF302843 mRNA. Translation: AAK28481.1 .
    AF302844 mRNA. Translation: AAK28482.1 .
    AF422177 mRNA. Translation: AAL17823.1 .
    CCDSi CCDS23496.1.
    RefSeqi NP_034619.2. NM_010489.2.
    XP_006511706.1. XM_006511643.1.
    XP_006511707.1. XM_006511644.1.
    XP_006511708.1. XM_006511645.1.
    UniGenei Mm.440982.
    Mm.4834.

    3D structure databases

    ProteinModelPortali O35632.
    SMRi O35632. Positions 29-440.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH56. Glycoside Hydrolase Family 56.

    Proteomic databases

    MaxQBi O35632.
    PRIDEi O35632.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000010191 ; ENSMUSP00000010191 ; ENSMUSG00000010047 .
    GeneIDi 15587.
    KEGGi mmu:15587.
    UCSCi uc009rlu.1. mouse.

    Organism-specific databases

    CTDi 8692.
    MGIi MGI:1196334. Hyal2.

    Phylogenomic databases

    eggNOGi NOG77606.
    HOGENOMi HOG000015133.
    HOVERGENi HBG052053.
    InParanoidi O35632.
    KOi K01197.
    OMAi GWGGEQC.
    OrthoDBi EOG74J97S.
    PhylomeDBi O35632.
    TreeFami TF321598.

    Enzyme and pathway databases

    Reactomei REACT_196554. Hyaluronan uptake and degradation.

    Miscellaneous databases

    NextBioi 288580.
    PROi O35632.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O35632.
    Bgeei O35632.
    CleanExi MM_HYAL2.
    Genevestigatori O35632.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR000742. EG-like_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR018155. Hyaluronidase.
    [Graphical view ]
    PANTHERi PTHR11769. PTHR11769. 1 hit.
    Pfami PF01630. Glyco_hydro_56. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038193. Hyaluronidase. 1 hit.
    PRINTSi PR00846. GLHYDRLASE56.
    SMARTi SM00181. EGF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00022. EGF_1. 1 hit.
    PS01186. EGF_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural organization and chromosomal localization of Hyal2, a gene encoding a lysosomal hyaluronidase."
      Strobl B., Wechselberger C., Beier D., Lepperdinger G.
      Genomics 53:214-219(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY.
      Strain: 129/SvJ.
    2. "Candidate tumor suppressor HYAL2 is a glycosylphosphatidylinositol (GPI)-anchored cell-surface receptor for jaagsiekte sheep retrovirus, the envelope protein of which mediates oncogenic transformation."
      Rai S.K., Duh F.-M., Vigdorovich V., Danilkovitch-Miagkova A., Lerman M.I., Miller A.D.
      Proc. Natl. Acad. Sci. U.S.A. 98:4443-4448(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C3H and Czech II.
    3. "Transforming growth factor-beta1 blocks the enhancement of tumor necrosis factor cytotoxicity by hyaluronidase Hyal-2 in L929 fibroblasts."
      Chang N.-S.
      BMC Cell Biol. 3:8-8(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Czech II.
    4. Cited for: REVIEW.
    5. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
      Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
      Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-74 AND ASN-390.

    Entry informationi

    Entry nameiHYAL2_MOUSE
    AccessioniPrimary (citable) accession number: O35632
    Secondary accession number(s): O35631, Q99MS9, Q99MT0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 15, 2002
    Last sequence update: November 15, 2002
    Last modified: October 1, 2014
    This is version 127 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    Was originally thought to be lysosomal.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3