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Protein

Hyaluronidase-2

Gene

Hyal2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R (By similarity).By similarity

Caution

Was originally thought to be lysosomal.Curated

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei135Proton donorBy similarity1

GO - Molecular functioni

  • enzyme binding Source: BHF-UCL
  • hyaluronic acid binding Source: UniProtKB
  • hyaluronoglucuronidase activity Source: UniProtKB
  • hyalurononglucosaminidase activity Source: UniProtKB
  • receptor signaling protein tyrosine kinase inhibitor activity Source: UniProtKB
  • receptor tyrosine kinase binding Source: MGI
  • transforming growth factor beta binding Source: BHF-UCL
  • virus receptor activity Source: UniProtKB

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • cartilage development Source: UniProtKB
  • cellular response to fibroblast growth factor stimulus Source: UniProtKB
  • cellular response to interleukin-1 Source: UniProtKB
  • cellular response to transforming growth factor beta stimulus Source: BHF-UCL
  • cellular response to tumor necrosis factor Source: Ensembl
  • cellular response to UV-B Source: UniProtKB
  • defense response to virus Source: UniProtKB
  • fusion of virus membrane with host plasma membrane Source: UniProtKB
  • glycosaminoglycan catabolic process Source: UniProtKB
  • hematopoietic progenitor cell differentiation Source: MGI
  • hyaluronan catabolic process Source: UniProtKB
  • monocyte activation Source: UniProtKB
  • multicellular organism aging Source: MGI
  • multicellular organismal iron ion homeostasis Source: MGI
  • negative regulation of cell growth Source: UniProtKB
  • negative regulation of fibroblast migration Source: UniProtKB
  • negative regulation of MAP kinase activity Source: UniProtKB
  • negative regulation of protein kinase B signaling Source: UniProtKB
  • negative regulation of protein tyrosine kinase activity Source: UniProtKB
  • positive regulation of extrinsic apoptotic signaling pathway Source: MGI
  • positive regulation of inflammatory response Source: UniProtKB
  • positive regulation of interleukin-6 secretion Source: UniProtKB
  • positive regulation of interleukin-8 secretion Source: UniProtKB
  • positive regulation of protein import into nucleus Source: BHF-UCL
  • positive regulation of transcription by RNA polymerase II Source: BHF-UCL
  • positive regulation of urine volume Source: UniProtKB
  • renal water absorption Source: UniProtKB
  • response to antibiotic Source: Ensembl
  • response to reactive oxygen species Source: UniProtKB
  • response to virus Source: UniProtKB
  • skeletal system morphogenesis Source: MGI
  • transcription by RNA polymerase II Source: GOC
  • transformation of host cell by virus Source: UniProtKB
  • viral entry into host cell Source: UniProtKB

Keywordsi

Molecular functionGlycosidase, Hydrolase, Receptor

Enzyme and pathway databases

BRENDAi3.2.1.35 3474
ReactomeiR-MMU-2160916 Hyaluronan uptake and degradation

Protein family/group databases

CAZyiGH56 Glycoside Hydrolase Family 56

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronidase-2 (EC:3.2.1.35)
Short name:
Hyal-2
Alternative name(s):
Hyaluronoglucosaminidase-2
Gene namesi
Name:Hyal2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1196334 Hyal2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000001210121 – 448Hyaluronidase-2Add BLAST428
PropeptideiPRO_0000012102449 – 473Removed in mature formSequence analysisAdd BLAST25

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi47 ↔ 340By similarity
Glycosylationi74N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi103N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi211 ↔ 227By similarity
Glycosylationi357N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi365 ↔ 376By similarity
Disulfide bondi370 ↔ 427By similarity
Glycosylationi390N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi429 ↔ 438By similarity
Lipidationi448GPI-anchor amidated asparagine; alternateSequence analysis1
Glycosylationi448N-linked (GlcNAc...) asparagine; alternateSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiO35632
PeptideAtlasiO35632
PRIDEiO35632

PTM databases

iPTMnetiO35632
PhosphoSitePlusiO35632

Expressioni

Tissue specificityi

Widely expressed. In the brain, expressed during embryonic stages but expression decreases after birth and is barely detectable in adult brain.1 Publication

Gene expression databases

BgeeiENSMUSG00000010047
CleanExiMM_HYAL2
ExpressionAtlasiO35632 baseline and differential
GenevisibleiO35632 MM

Interactioni

Subunit structurei

Interacts with MST1R.By similarity

GO - Molecular functioni

  • enzyme binding Source: BHF-UCL
  • receptor tyrosine kinase binding Source: MGI
  • transforming growth factor beta binding Source: BHF-UCL

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000010191

Structurei

3D structure databases

ProteinModelPortaliO35632
SMRiO35632
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini361 – 439EGF-likeAdd BLAST79

Sequence similaritiesi

Belongs to the glycosyl hydrolase 56 family.Curated

Keywords - Domaini

EGF-like domain, Signal

Phylogenomic databases

eggNOGiENOG410IECJ Eukaryota
ENOG410XPZT LUCA
GeneTreeiENSGT00550000074476
HOGENOMiHOG000015133
HOVERGENiHBG052053
InParanoidiO35632
KOiK01197
OMAiWGGEQCQ
OrthoDBiEOG091G064G
PhylomeDBiO35632
TreeFamiTF321598

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR017853 Glycoside_hydrolase_SF
IPR018155 Hyaluronidase
PANTHERiPTHR11769 PTHR11769, 1 hit
PfamiView protein in Pfam
PF01630 Glyco_hydro_56, 1 hit
PIRSFiPIRSF038193 Hyaluronidase, 1 hit
PRINTSiPR00846 GLHYDRLASE56
SUPFAMiSSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 1 hit
PS01186 EGF_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35632-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAGLGPIIT LALVLEVAWA GELKPTAPPI FTGRPFVVAW NVPTQECAPR
60 70 80 90 100
HKVPLDLRAF DVKATPNEGF FNQNITTFYY DRLGLYPRFD AAGTSVHGGV
110 120 130 140 150
PQNGSLCAHL PMLKESVERY IQTQEPGGLA VIDWEEWRPV WVRNWQEKDV
160 170 180 190 200
YRQSSRQLVA SRHPDWPSDR VMKQAQYEFE FAARQFMLNT LRYVKAVRPQ
210 220 230 240 250
HLWGFYLFPD CYNHDYVQNW ESYTGRCPDV EVARNDQLAW LWAESTALFP
260 270 280 290 300
SVYLDETLAS SVHSRNFVSF RVREALRVAH THHANHALPV YVFTRPTYTR
310 320 330 340 350
GLTGLSQVDL ISTIGESAAL GSAGVIFWGD SEDASSMETC QYLKNYLTQL
360 370 380 390 400
LVPYIVNVSW ATQYCSWTQC HGHGRCVRRN PSANTFLHLN ASSFRLVPGH
410 420 430 440 450
TPSEPQLRPE GQLSEADLNY LQKHFRCQCY LGWGGEQCQR NYKGAAGNAS
460 470
RAWAGSHLTS LLGLVAVALT WTL
Length:473
Mass (Da):53,618
Last modified:November 15, 2002 - v2
Checksum:iDD433C9FFCF8147C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26 – 27TA → KP in CAA03888 (PubMed:9790770).Curated2
Sequence conflicti26 – 27TA → KP in CAA03889 (PubMed:9790770).Curated2
Sequence conflicti193 – 199YVKAVRP → LRQGSQT in CAA03888 (PubMed:9790770).Curated7
Sequence conflicti250Missing in CAA03888 (PubMed:9790770).Curated1
Sequence conflicti269 – 272SFRV → RFGG in CAA03888 (PubMed:9790770).Curated4
Sequence conflicti355I → V in AAK28481 (PubMed:11296287).Curated1
Sequence conflicti383A → V in CAA03888 (PubMed:9790770).Curated1
Sequence conflicti383A → V in CAA03889 (PubMed:9790770).Curated1
Sequence conflicti416 – 422ADLNYLQ → RDRQLPE in CAA03888 (PubMed:9790770).Curated7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000059 mRNA Translation: CAA03888.1
AJ000060 Genomic DNA Translation: CAA03889.1
AF302843 mRNA Translation: AAK28481.1
AF302844 mRNA Translation: AAK28482.1
AF422177 mRNA Translation: AAL17823.1
CCDSiCCDS23496.1
RefSeqiNP_034619.2, NM_010489.2
XP_006511706.1, XM_006511643.3
XP_006511707.1, XM_006511644.3
XP_006511708.1, XM_006511645.3
UniGeneiMm.440982
Mm.4834

Genome annotation databases

EnsembliENSMUST00000010191; ENSMUSP00000010191; ENSMUSG00000010047
ENSMUST00000195752; ENSMUSP00000141280; ENSMUSG00000010047
GeneIDi15587
KEGGimmu:15587
UCSCiuc009rlu.1 mouse

Similar proteinsi

Entry informationi

Entry nameiHYAL2_MOUSE
AccessioniPrimary (citable) accession number: O35632
Secondary accession number(s): O35631, Q99MS9, Q99MT0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: November 15, 2002
Last modified: March 28, 2018
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health