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Protein

Nuclear receptor subfamily 1 group I member 3

Gene

Nr1i3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. Transactivates both the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei171Important for TCPOBOP recognition1
Sitei175Important for TCPOBOP recognition1
Sitei216Important for TCPOBOP recognition1
Sitei227Important for TCPOBOP recognition1
Sitei244Important for TCPOBOP recognition1
Sitei336Important for TCPOBOP recognition1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi18 – 93Nuclear receptorPROSITE-ProRule annotationAdd BLAST76
Zinc fingeri21 – 41NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri57 – 81NR C4-typePROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, Receptor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-383280 Nuclear Receptor transcription pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear receptor subfamily 1 group I member 3
Alternative name(s):
Constitutive androstane receptor
Short name:
CAR
Orphan nuclear receptor MB67
Gene namesi
Name:Nr1i3
Synonyms:Car
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1346307 Nr1i3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi171F → W: Diminished binding of coactivator NCOA2 in the presence of TCPOBOP. 1 Publication1
Mutagenesisi175N → F: Diminished binding of coactivator NCOA2 in the presence of TCPOBOP. 1 Publication1
Mutagenesisi216L → F: Diminished binding of coactivator NCOA2 in the presence of TCPOBOP. 1 Publication1
Mutagenesisi227F → W: Diminished binding of coactivator NCOA2 in the presence of TCPOBOP. 1 Publication1
Mutagenesisi234Y → A: No effect on binding of coactivator NCOA2 in the presence of TCPOBOP. 1 Publication1
Mutagenesisi244F → A: Diminished binding of coactivator NCOA2 in the presence of TCPOBOP. 1 Publication1
Mutagenesisi336Y → A: Diminished binding of coactivator NCOA2 in the presence of TCPOBOP. 1 Publication1
Mutagenesisi346L → F: Dramatic increase in binding NCOA2. Little effect on binding of coactivator NCOA2 in the presence of TCPOBOP. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL3069
GuidetoPHARMACOLOGYi607

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000535541 – 358Nuclear receptor subfamily 1 group I member 3Add BLAST358

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei48Phosphothreonine; by PKCBy similarity1

Post-translational modificationi

Phosphorylated at Thr-48 by PKC, dephosphorylation of Thr-48 is required for nuclear translocation and activation.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO35627
PRIDEiO35627

PTM databases

iPTMnetiO35627
PhosphoSitePlusiO35627

Expressioni

Tissue specificityi

Predominantly expressed in liver.

Gene expression databases

BgeeiENSMUSG00000005677
CleanExiMM_NR1I3
ExpressionAtlasiO35627 baseline and differential
GenevisibleiO35627 MM

Interactioni

Subunit structurei

Heterodimer of NR1I3 and RXR. Interacts with PSMC4. Interacts with ECT2. Directly interacts with DNAJC7; this complex may also include HSP90.4 Publications

Protein-protein interaction databases

BioGridi198489, 13 interactors
STRINGi10090.ENSMUSP00000005820

Chemistry databases

BindingDBiO35627

Structurei

Secondary structure

1358
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi118 – 134Combined sources17
Turni135 – 137Combined sources3
Helixi138 – 144Combined sources7
Helixi149 – 152Combined sources4
Beta strandi160 – 162Combined sources3
Helixi164 – 187Combined sources24
Helixi190 – 193Combined sources4
Helixi197 – 216Combined sources20
Helixi217 – 219Combined sources3
Turni222 – 225Combined sources4
Beta strandi226 – 229Combined sources4
Beta strandi232 – 234Combined sources3
Helixi236 – 241Combined sources6
Helixi246 – 260Combined sources15
Helixi266 – 277Combined sources12
Helixi288 – 308Combined sources21
Turni309 – 312Combined sources4
Helixi318 – 335Combined sources18
Helixi337 – 343Combined sources7
Helixi345 – 349Combined sources5
Helixi351 – 357Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XLSX-ray2.96E/F/G/H117-358[»]
1XNXX-ray2.90A/B109-358[»]
ProteinModelPortaliO35627
SMRiO35627
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO35627

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini119 – 358NR LBDPROSITE-ProRule annotationAdd BLAST240

Domaini

Composed by a short N-terminal domain followed by the DNA binding, hinge, and ligand binding/dimerization domains.

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri21 – 41NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri57 – 81NR C4-typePROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3575 Eukaryota
ENOG410XRZC LUCA
GeneTreeiENSGT00870000136372
HOGENOMiHOG000220844
HOVERGENiHBG108655
InParanoidiO35627
KOiK08541
OMAiPRDRFLY
OrthoDBiEOG091G0DMU
TreeFamiTF316304

Family and domain databases

Gene3Di3.30.50.10, 1 hit
InterProiView protein in InterPro
IPR035500 NHR_like_dom_sf
IPR000536 Nucl_hrmn_rcpt_lig-bd
IPR001723 Nuclear_hrmn_rcpt
IPR001728 ThyrH_rcpt
IPR001628 Znf_hrmn_rcpt
IPR013088 Znf_NHR/GATA
PfamiView protein in Pfam
PF00104 Hormone_recep, 1 hit
PF00105 zf-C4, 1 hit
PRINTSiPR00398 STRDHORMONER
PR00047 STROIDFINGER
PR00546 THYROIDHORMR
SMARTiView protein in SMART
SM00430 HOLI, 1 hit
SM00399 ZnF_C4, 1 hit
SUPFAMiSSF48508 SSF48508, 1 hit
PROSITEiView protein in PROSITE
PS51843 NR_LBD, 1 hit
PS00031 NUCLEAR_REC_DBD_1, 1 hit
PS51030 NUCLEAR_REC_DBD_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform CAR1 (identifier: O35627-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTAMLTLETM ASEEEYGPRN CVVCGDRATG YHFHALTCEG CKGFFRRTVS
60 70 80 90 100
KTIGPICPFA GRCEVSKAQR RHCPACRLQK CLNVGMRKDM ILSAEALALR
110 120 130 140 150
RARQAQRRAE KASLQLNQQQ KELVQILLGA HTRHVGPMFD QFVQFKPPAY
160 170 180 190 200
LFMHHRPFQP RGPVLPLLTH FADINTFMVQ QIIKFTKDLP LFRSLTMEDQ
210 220 230 240 250
ISLLKGAAVE ILHISLNTTF CLQTENFFCG PLCYKMEDAV HAGFQYEFLE
260 270 280 290 300
SILHFHKNLK GLHLQEPEYV LMAATALFSP DRPGVTQREE IDQLQEEMAL
310 320 330 340 350
ILNNHIMEQQ SRLQSRFLYA KLMGLLADLR SINNAYSYEL QRLEELSAMT

PLLGEICS
Length:358
Mass (Da):40,913
Last modified:July 27, 2011 - v2
Checksum:iA11C92B1EC2C06A7
GO
Isoform CAR2 (identifier: O35627-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     281-286: DRPGVT → GFCMQS
     287-358: Missing.

Note: Does not seem to act as a transactivator. Lacks the C-terminal portion of the ligand binding/dimerization domain.
Show »
Length:286
Mass (Da):32,544
Checksum:iDAEF17A3369F529E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti138M → L in AAC53349 (PubMed:9295294).Curated1
Sequence conflicti138M → L in AAC53350 (PubMed:9295294).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003671281 – 286DRPGVT → GFCMQS in isoform CAR2. 1 Publication6
Alternative sequenceiVSP_003672287 – 358Missing in isoform CAR2. 1 PublicationAdd BLAST72

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF009327 mRNA Translation: AAC53349.1
AF009328 mRNA Translation: AAC53350.1
AK133515 mRNA Translation: BAE21697.1
CH466520 Genomic DNA Translation: EDL39126.1
CCDSiCCDS15480.1 [O35627-1]
CCDS56654.1 [O35627-2]
RefSeqiNP_001229991.1, NM_001243062.1 [O35627-2]
NP_001229992.1, NM_001243063.1
NP_033933.2, NM_009803.5 [O35627-1]
UniGeneiMm.486506

Genome annotation databases

EnsembliENSMUST00000005820; ENSMUSP00000005820; ENSMUSG00000005677 [O35627-1]
ENSMUST00000075469; ENSMUSP00000074915; ENSMUSG00000005677 [O35627-2]
GeneIDi12355
KEGGimmu:12355
UCSCiuc007dnf.2 mouse [O35627-1]
uc007dng.2 mouse [O35627-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNR1I3_MOUSE
AccessioniPrimary (citable) accession number: O35627
Secondary accession number(s): O35628, Q3V008
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 27, 2011
Last modified: March 28, 2018
This is version 167 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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