##gff-version 3 O35625 UniProtKB Chain 1 863 . . . ID=PRO_0000220889;Note=Axin-1 O35625 UniProtKB Domain 88 211 . . . Note=RGS;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00171 O35625 UniProtKB Domain 781 863 . . . Note=DIX;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00069 O35625 UniProtKB Region 1 81 . . . Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite O35625 UniProtKB Region 209 338 . . . Note=Interaction with TP53;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15526030;Dbxref=PMID:15526030 O35625 UniProtKB Region 215 240 . . . Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite O35625 UniProtKB Region 249 268 . . . Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite O35625 UniProtKB Region 315 344 . . . Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite O35625 UniProtKB Region 348 432 . . . Note=Interaction with GSK3B;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:O70239 O35625 UniProtKB Region 353 411 . . . Note=Interaction with SIAH1;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:O15169 O35625 UniProtKB Region 433 501 . . . Note=Interaction with beta-catenin;Ontology_term=ECO:0000250;evidence=ECO:0000250 O35625 UniProtKB Region 505 758 . . . Note=Interaction with RNF111;Ontology_term=ECO:0000250;evidence=ECO:0000250 O35625 UniProtKB Region 529 624 . . . Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite O35625 UniProtKB Region 572 790 . . . Note=Interaction with PPP2CA;Ontology_term=ECO:0000250;evidence=ECO:0000250 O35625 UniProtKB Region 642 664 . . . Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite O35625 UniProtKB Region 678 753 . . . Note=Interaction with HIPK2;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15526030;Dbxref=PMID:15526030 O35625 UniProtKB Motif 20 29 . . . Note=Tankyrase-binding motif O35625 UniProtKB Compositional bias 36 63 . . . Note=Polar residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite O35625 UniProtKB Compositional bias 215 235 . . . Note=Polar residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite O35625 UniProtKB Compositional bias 315 339 . . . Note=Polar residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite O35625 UniProtKB Modified residue 75 75 . . . Note=Phosphoserine;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:21183079;Dbxref=PMID:21183079 O35625 UniProtKB Modified residue 77 77 . . . Note=Phosphoserine%3B by CK1;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:O15169 O35625 UniProtKB Modified residue 217 217 . . . Note=Phosphoserine;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:21183079;Dbxref=PMID:21183079 O35625 UniProtKB Modified residue 468 468 . . . Note=Phosphoserine%3B by CK1;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:O15169 O35625 UniProtKB Modified residue 480 480 . . . Note=Phosphothreonine%3B by GSK3-beta;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:10581160;Dbxref=PMID:10581160 O35625 UniProtKB Modified residue 485 485 . . . Note=Phosphoserine%3B by GSK3-beta;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10581160;Dbxref=PMID:10581160 O35625 UniProtKB Modified residue 492 492 . . . Note=Phosphoserine;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10581160;Dbxref=PMID:10581160 O35625 UniProtKB Modified residue 509 509 . . . Note=Phosphoserine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:O15169 O35625 UniProtKB Cross-link 858 858 . . . Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO);Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:12223491,ECO:0000269|PubMed:18632848;Dbxref=PMID:12223491,PMID:18632848 O35625 UniProtKB Cross-link 861 861 . . . Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO);Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:12223491,ECO:0000269|PubMed:18632848;Dbxref=PMID:12223491,PMID:18632848 O35625 UniProtKB Alternative sequence 731 766 . . . ID=VSP_000452;Note=In isoform 2. Missing;Ontology_term=ECO:0000303;evidence=ECO:0000303|PubMed:9230313;Dbxref=PMID:9230313 O35625 UniProtKB Mutagenesis 480 480 . . . Note=Greatly reduced GSK3B-mediated phosphorylation%3B when associated with A-485 and A-492. T->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10581160;Dbxref=PMID:10581160 O35625 UniProtKB Mutagenesis 485 485 . . . Note=Greatly reduced GSK3B-mediated phosphorylation and large effect on the inhibitory activity in Wnt-signaling%3B when associated with A-480 and A-492. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10581160;Dbxref=PMID:10581160 O35625 UniProtKB Mutagenesis 492 492 . . . Note=Greatly reduced GSK3B-mediated phosphorylation%3B when associated with A-480 and A-485. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10581160;Dbxref=PMID:10581160 O35625 UniProtKB Mutagenesis 492 492 . . . Note=Little effect on inhibitory activity on Wnt-signaling. S->I;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10581160;Dbxref=PMID:10581160 O35625 UniProtKB Mutagenesis 495 495 . . . Note=No effect on phosphorylation. Little effect on inhibitory activity on Wnt-signaling. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10581160;Dbxref=PMID:10581160 O35625 UniProtKB Mutagenesis 858 863 . . . Note=Abolishes binding of PIAS1 and PIAS2. Dramatically reduces sumoylation and abolishes AXIN1-mediated JNK activation. No effect on homodimerization nor on Wnt-signaling. Missing;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12223491;Dbxref=PMID:12223491 O35625 UniProtKB Mutagenesis 858 858 . . . Note=Decreased sumoylation followed by increased ubiquitination%3B when associated with A-861. K->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:18632848;Dbxref=PMID:18632848 O35625 UniProtKB Mutagenesis 861 861 . . . Note=Decreased sumoylation followed by increased ubiquitination%3B when associated with A-858. K->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:18632848;Dbxref=PMID:18632848 O35625 UniProtKB Sequence conflict 589 589 . . . Note=A->P;Ontology_term=ECO:0000305;evidence=ECO:0000305 O35625 UniProtKB Sequence conflict 787 787 . . . Note=A->G;Ontology_term=ECO:0000305;evidence=ECO:0000305 O35625 UniProtKB Sequence conflict 846 846 . . . Note=A->P;Ontology_term=ECO:0000305;evidence=ECO:0000305 O35625 UniProtKB Beta strand 64 66 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3UTM