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Reviewed, UniProtKB/Swiss-Prot O35625 (AXN1_MOUSE)

Last modified January 19, 2010. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Axin-1
Alternative name(s):
    Axis inhibition protein 1
    Protein Fused
Gene names
Name: Axin1
Synonyms: Axin, Fu
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length863 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Controls dorsoventral patterning via two opposing effects; down-regulates beta-catenin to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of beta-catenin and APC by GSK3B. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7 By similarity. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Ref.3 Ref.5

Subunit structure

Homodimer. Interacts with GSK3B and beta-catenin. The interaction between axin and beta-catenin occurs via the armadillo repeats contained in beta-catenin. Ternary complex. Also binds to plakoglobin (gamma-catenin), APC, DVL and PP2A. Interacts with SMAD6, SMAD7 and RNF111 By similarity. Interacts with TP53 and HIPK2. Probably part of a complex consisting of TP53, HIPK2 and AXIN1. Interacts with DIXDC1; prevents interaction with MAP3K1. Interacts with MAP3K4, MDFI and MDFIC. Interacts with ANKRD6. Interacts with AIDA. Ref.3 Ref.5 Ref.2 Ref.4

Subcellular location

Cytoplasm By similarity.

Tissue specificity

Expressed in embryonic stem cells.

Developmental stage

Widely expressed at E10.5 to E16.5 day.

Post-translational modification

Probably phosphorylated by GSK3B and dephosphorylated by PP2A. Ref.6

Sequence similarities

Contains 1 DIX domain.

Contains 1 RGS domain.

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O35625-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O35625-2)

The sequence of this isoform differs from the canonical sequence as follows:
     731-766: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 863863Axin-1
PRO_0000220889

Regions

Domain88 – 211124RGS
Domain781 – 86383DIX
Region209 – 338130Interaction with TP53
Region348 – 43285Interaction with GSK3B By similarity
Region433 – 50169Interaction with beta-catenin By similarity
Region505 – 758254Interaction with RNF111 By similarity
Region678 – 75376Interaction with HIPK2

Amino acid modifications

Modified residue4921Phosphoserine Ref.6

Natural variations

Alternative sequence731 – 76636Missing in isoform 2.
VSP_000452

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 10, 2002. Version 2.
Checksum: AD419D685411E9FB

FASTA86396,314
        10         20         30         40         50         60 
MNVQEQGFPL DLGASFTEDA PRPPVPGEEG ELVSTDSRPV NHSFCSGKGT SIKSETSTAT 

        70         80         90        100        110        120 
PRRSDLDLGY EPEGSASPTP PYLRWAESLH SLLDDQDGIS LFRTFLKQEG CADLLDFWFA 

       130        140        150        160        170        180 
CSGFRKLEPC DSNEEKRLKL ARAIYRKYIL DSNGIVSRQT KPATKSFIKD CVMKQQIDPA 

       190        200        210        220        230        240 
MFDQAQTEIQ STMEENTYPS FLKSDIYLEY TRTGSESPKV CSDQSSGSGT GKGMSGYLPT 

       250        260        270        280        290        300 
LNEDEEWKCD QDADEDDGRD PLPPSRLTQK LLLETAAPRA PSSRRYNEGR ELRYGSWREP 

       310        320        330        340        350        360 
VNPYYVNSGY ALAPATSAND SEQQSLSSDA DTLSLTDSSV DGIPPYRIRK QHRREMQESI 

       370        380        390        400        410        420 
QVNGRVPLPH IPRTYRMPKE IRVEPQKFAE ELIHRLEAVQ RTREAEEKLE ERLKRVRMEE 

       430        440        450        460        470        480 
EGEDGEMPSG PMASHKLPSV PAWHHFPPRY VDMGCSGLRD AHEENPESIL DEHVQRVMRT 

       490        500        510        520        530        540 
PGCQSPGPGH RSPDSGHVAK TAVLGGTASG HGKHVPKLGL KLDTAGLHHH RHVHHHVHHN 

       550        560        570        580        590        600 
SARPKEQMEA EVARRVQSSF SWGPETHGHA KPRSYSENAG TTLSAGDLPF GGKTSAPSKR 

       610        620        630        640        650        660 
NTKKAESGKN ANAEVPSTTE DAEKNQKIMQ WIIEGEKEIS RHRKAGHGSS GLRKQQAHES 

       670        680        690        700        710        720 
SRPLSIERPG AVHPWVSAQL RNSVQPSHLF IQDPTMPPNP APNPLTQLEE ARRRLEEEEK 

       730        740        750        760        770        780 
RANKLPSKQR YVQAVMQRGR TCVRPACAPV LSVVPAVSDL ELSETETKSQ RKAGGGSAPP 

       790        800        810        820        830        840 
CDSIVVGYYF CGEPIPYRTL VRGRAVTLGQ FKELLTKKGS YRYYFKKVSD EFDCGVVFEE 

       850        860 
VREDEPVLPV FEEKIIGKVE KVD

« Hide

Isoform 2.

Checksum: 091354285F9EAF9E
Show »

FASTA82792,456

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References

« Hide 'large scale' references
[1]"The mouse Fused locus encodes Axin, an inhibitor of the Wnt signaling pathway that regulates embryonic axis formation."
Zeng L., Fagotto F., Zhang T., Hsu W., Vasicek T.J., Perry W.L. III, Lee J.J., Tilghman S.M., Gumbiner B.M., Costantini F.
Cell 90:181-192(1997) [PubMed: 9230313] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
[2]"The ankyrin repeat protein diversin recruits casein kinase Iepsilon to the beta-catenin degradation complex and acts in both canonical Wnt and Wnt/JNK signaling."
Schwarz-Romond T., Asbrand C., Bakkers J., Kuehl M., Schaeffer H.J., Huelsken J., Behrens J., Hammerschmidt M., Birchmeier W.
Genes Dev. 16:2073-2084(2002) [PubMed: 12183362] [Abstract]
Cited for: INTERACTION WITH ANKRD6.
[3]"Axin stimulates p53 functions by activation of HIPK2 kinase through multimeric complex formation."
Rui Y., Xu Z., Lin S., Li Q., Rui H., Luo W., Zhou H.-M., Cheung P.-Y., Wu Z., Ye Z., Li P., Han J., Lin S.-C.
EMBO J. 23:4583-4594(2004) [PubMed: 15526030] [Abstract]
Cited for: FUNCTION, INTERACTION WITH TP53 AND HIPK2, IDENTIFICATION IN A COMPLEX WITH TP53 AND HIPK2.
[4]"The DIX domain protein coiled-coil-DIX1 inhibits c-Jun N-terminal kinase activation by Axin and dishevelled through distinct mechanisms."
Wong C.K., Luo W., Deng Y., Zou H., Ye Z., Lin S.-C.
J. Biol. Chem. 279:39366-39373(2004) [PubMed: 15262978] [Abstract]
Cited for: INTERACTION WITH DIXDC1; MAP3K1 AND MAP3K4.
[5]"A beta-catenin-independent dorsalization pathway activated by Axin/JNK signaling and antagonized by aida."
Rui Y., Xu Z., Xiong B., Cao Y., Lin S., Zhang M., Chan S.-C., Luo W., Han Y., Lu Z., Ye Z., Zhou H.-M., Han J., Meng A., Lin S.-C.
Dev. Cell 13:268-282(2007) [PubMed: 17681137] [Abstract]
Cited for: FUNCTION, HOMODIMERIZATION, INTERACTION WITH AIDA.
[6]"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry."
Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M.
J. Proteome Res. 7:5314-5326(2008) [PubMed: 18973353] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-492, MASS SPECTROMETRY.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF009011 mRNA. Translation: AAC53285.1. Different initiation.
IPIIPI00845819.
IPI00875849.
RefSeqNP_033863.2.
UniGeneMm.23684

3D structure databases

SMRO35625. Positions 74-220, 780-863.
ModBaseSearch...

Protein-protein interaction databases

IntActO35625. 2 interactions.
STRINGO35625.

PTM databases

PhosphoSiteO35625.

Proteomic databases

PRIDEO35625.

Genome annotation databases

EnsemblENSMUST00000074370; ENSMUSP00000073974; ENSMUSG00000024182; Mus musculus. [Genome view]
ENSMUST00000115005; ENSMUSP00000110657; ENSMUSG00000024182; Mus musculus. [Genome view]
GeneID12005.
UCSCuc008bdm.1. mouse.

Organism-specific databases

CTD12005.
MGIMGI:1096327. Axin1.

Phylogenomic databases

eggNOGroNOG11401.
HOGENOMHBG714290.
HOVERGENO35625.
InParanoidO35625.

Gene expression databases

ArrayExpressO35625.
BgeeO35625.
CleanExMM_AXIN1.
GenevestigatorO35625.
GermOnlineENSMUSG00000024182. Mus musculus.

Family and domain databases

InterProIPR014936. Axin_b-cat_bd.
IPR001158. DIX.
IPR000342. Regulat_G_prot_signal.
IPR016137. Regulat_G_prot_signal_superfam.
[Graphical view]
PfamPF08833. Axin_b-cat_bind. 1 hit.
PF00778. DIX. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSPR01301. RGSPROTEIN.
SMARTSM00021. DAX. 1 hit.
SM00315. RGS. 1 hit.
[Graphical view]
PROSITEPS50841. DIX. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio280195.
SOURCESearch...

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Entry information

Entry nameAXN1_MOUSE
AccessionPrimary (citable) accession number: O35625
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 10, 2002
Last modified: January 19, 2010
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents