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O35621

- PMM1_MOUSE

UniProt

O35621 - PMM1_MOUSE

Protein

Phosphomannomutase 1

Gene

Pmm1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. In addition, may be responsible for the degradation of glucose-1,6-bisphosphate in ischemic brain.1 Publication

    Catalytic activityi

    Alpha-D-mannose 1-phosphate = D-mannose 6-phosphate.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    IMP, a metabolite whose concentration is elevated in anoxia, inhibits phosphomannomutase and phosphoglucomutase activities and strongly enhances glucose-1,6-bisphosphatase activity.1 Publication

    Kineticsi

    1. KM=17 µM for glucose-1,6-bisphosphate in the presence of 1 µM IMP1 Publication
    2. KM=40 µM for glucose-1,6-bisphosphate in the presence of 20 µM IMP1 Publication

    Vmax=2.1 µmol/min/mg enzyme with glucose-1,6-bisphosphate as substrate1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei19 – 191NucleophileBy similarity
    Metal bindingi19 – 191MagnesiumBy similarity
    Active sitei21 – 211Proton donor/acceptorSequence Analysis
    Metal bindingi21 – 211Magnesium; via carbonyl oxygenBy similarity
    Binding sitei28 – 281SubstrateBy similarity
    Binding sitei132 – 1321SubstrateBy similarity
    Binding sitei143 – 1431SubstrateBy similarity
    Binding sitei150 – 1501SubstrateBy similarity
    Binding sitei186 – 1861Substrate; via amide nitrogenBy similarity
    Binding sitei188 – 1881SubstrateBy similarity
    Binding sitei190 – 1901SubstrateBy similarity
    Metal bindingi218 – 2181MagnesiumBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. phosphomannomutase activity Source: UniProtKB

    GO - Biological processi

    1. GDP-mannose biosynthetic process Source: UniProtKB-UniPathway
    2. mannose biosynthetic process Source: InterPro
    3. mannose metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Isomerase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_198715. Synthesis of GDP-mannose.
    UniPathwayiUPA00126; UER00424.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphomannomutase 1 (EC:5.4.2.8)
    Short name:
    PMM 1
    Gene namesi
    Name:Pmm1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:1353418. Pmm1.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. neuronal cell body Source: MGI

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Mice are viable and fertile and develop normally.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 262261Phosphomannomutase 1PRO_0000199693Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiO35621.
    PaxDbiO35621.
    PRIDEiO35621.

    PTM databases

    PhosphoSiteiO35621.

    Expressioni

    Tissue specificityi

    Present in brain, where it is restricted to neuronal cell bodies. Present at lower levels in pancreas, liver, lung, gonads, uterus, adrenal glands and pituitary (at protein level). Undetectable in intestine.1 Publication

    Developmental stagei

    Highly expressed at E17 in most organs (at protein level).1 Publication

    Gene expression databases

    ArrayExpressiO35621.
    BgeeiO35621.
    CleanExiMM_PMM1.
    GenevestigatoriO35621.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO35621.
    SMRiO35621. Positions 13-258.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the eukaryotic PMM family.Curated

    Phylogenomic databases

    eggNOGiCOG0561.
    HOGENOMiHOG000181843.
    HOVERGENiHBG009971.
    InParanoidiO35621.
    KOiK17497.
    OMAiNDYEIYD.
    OrthoDBiEOG773XH0.
    PhylomeDBiO35621.
    TreeFamiTF300874.

    Family and domain databases

    Gene3Di3.40.50.1000. 2 hits.
    InterProiIPR023214. HAD-like_dom.
    IPR006379. HAD-SF_hydro_IIB.
    IPR005002. PMM.
    [Graphical view]
    PANTHERiPTHR10466. PTHR10466. 1 hit.
    PfamiPF03332. PMM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR01484. HAD-SF-IIB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O35621-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVAVEGARR KERILCLFDV DGTLTPARQK IDPEVSAFLQ KLRSRVQIGV    50
    VGGSDYSKIA EQLGEGDEVI EKFDYVFAEN GTVQYKHGRL LSKQTIQNHL 100
    GEELLQDLIN FCLSYMALLR LPKKRGTFIE FRNGMLNVSP IGRSCTLEER 150
    IEFSELDKKE KIREKFVEAL KTEFAGKGLR FSRGGMISFD VFPEGWDKRY 200
    CLDSLDEDSF DIIHFFGNET SPGGNDFEIY ADPRTVGHSV VSPQDTVQRC 250
    RELFFPETAH EA 262
    Length:262
    Mass (Da):29,775
    Last modified:January 1, 1998 - v1
    Checksum:i2967154C0FF292B0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF007267 mRNA. Translation: AAB62943.1.
    CCDSiCCDS27678.1.
    RefSeqiNP_038900.1. NM_013872.4.
    UniGeneiMm.18939.

    Genome annotation databases

    EnsembliENSMUST00000023112; ENSMUSP00000023112; ENSMUSG00000022474.
    GeneIDi29858.
    KEGGimmu:29858.
    UCSCiuc007wxt.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF007267 mRNA. Translation: AAB62943.1 .
    CCDSi CCDS27678.1.
    RefSeqi NP_038900.1. NM_013872.4.
    UniGenei Mm.18939.

    3D structure databases

    ProteinModelPortali O35621.
    SMRi O35621. Positions 13-258.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei O35621.

    Proteomic databases

    MaxQBi O35621.
    PaxDbi O35621.
    PRIDEi O35621.

    Protocols and materials databases

    DNASUi 29858.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000023112 ; ENSMUSP00000023112 ; ENSMUSG00000022474 .
    GeneIDi 29858.
    KEGGi mmu:29858.
    UCSCi uc007wxt.2. mouse.

    Organism-specific databases

    CTDi 5372.
    MGIi MGI:1353418. Pmm1.

    Phylogenomic databases

    eggNOGi COG0561.
    HOGENOMi HOG000181843.
    HOVERGENi HBG009971.
    InParanoidi O35621.
    KOi K17497.
    OMAi NDYEIYD.
    OrthoDBi EOG773XH0.
    PhylomeDBi O35621.
    TreeFami TF300874.

    Enzyme and pathway databases

    UniPathwayi UPA00126 ; UER00424 .
    Reactomei REACT_198715. Synthesis of GDP-mannose.

    Miscellaneous databases

    ChiTaRSi PMM1. mouse.
    NextBioi 307058.
    PROi O35621.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O35621.
    Bgeei O35621.
    CleanExi MM_PMM1.
    Genevestigatori O35621.

    Family and domain databases

    Gene3Di 3.40.50.1000. 2 hits.
    InterProi IPR023214. HAD-like_dom.
    IPR006379. HAD-SF_hydro_IIB.
    IPR005002. PMM.
    [Graphical view ]
    PANTHERi PTHR10466. PTHR10466. 1 hit.
    Pfami PF03332. PMM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 1 hit.
    TIGRFAMsi TIGR01484. HAD-SF-IIB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Janoueix-Lerosey I., Goud B.
      Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The normal phenotype of Pmm1-deficient mice suggests that Pmm1 is not essential for normal mouse development."
      Cromphout K., Vleugels W., Heykants L., Schollen E., Keldermans L., Sciot R., D'Hooge R., De Deyn P.P., von Figura K., Hartmann D., Korner C., Matthijs G.
      Mol. Cell. Biol. 26:5621-5635(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
    3. "Mammalian phosphomannomutase PMM1 is the brain IMP-sensitive glucose-1,6-bisphosphatase."
      Veiga-da-Cunha M., Vleugels W., Maliekal P., Matthijs G., Van Schaftingen E.
      J. Biol. Chem. 283:33988-33993(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiPMM1_MOUSE
    AccessioniPrimary (citable) accession number: O35621
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 24, 2001
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3