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Protein

Phosphomannomutase 1

Gene

Pmm1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. In addition, may be responsible for the degradation of glucose-1,6-bisphosphate in ischemic brain.1 Publication

Catalytic activityi

Alpha-D-mannose 1-phosphate = D-mannose 6-phosphate.

Cofactori

Mg2+By similarity

Enzyme regulationi

IMP, a metabolite whose concentration is elevated in anoxia, inhibits phosphomannomutase and phosphoglucomutase activities and strongly enhances glucose-1,6-bisphosphatase activity.1 Publication

Kineticsi

  1. KM=17 µM for glucose-1,6-bisphosphate in the presence of 1 µM IMP1 Publication
  2. KM=40 µM for glucose-1,6-bisphosphate in the presence of 20 µM IMP1 Publication
  1. Vmax=2.1 µmol/min/mg enzyme with glucose-1,6-bisphosphate as substrate1 Publication

Pathway: GDP-alpha-D-mannose biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Mannose-6-phosphate isomerase (Mpi), Mannose-6-phosphate isomerase (Mpi), Mannose-6-phosphate isomerase (Mpi)
  2. Phosphomannomutase 1 (Pmm1), Phosphomannomutase (Pmm2), Phosphomannomutase, Phosphomannomutase (Pmm2), Phosphomannomutase (Pmm1), Phosphomannomutase (Pmm2), Phosphomannomutase (Pmm1), Phosphomannomutase (Pmm2), Phosphomannomutase 2 (Pmm2)
This subpathway is part of the pathway GDP-alpha-D-mannose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate, the pathway GDP-alpha-D-mannose biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei19 – 191NucleophileBy similarity
Metal bindingi19 – 191MagnesiumBy similarity
Active sitei21 – 211Proton donor/acceptorSequence Analysis
Metal bindingi21 – 211Magnesium; via carbonyl oxygenBy similarity
Binding sitei28 – 281SubstrateBy similarity
Binding sitei132 – 1321SubstrateBy similarity
Binding sitei143 – 1431SubstrateBy similarity
Binding sitei150 – 1501SubstrateBy similarity
Binding sitei186 – 1861Substrate; via amide nitrogenBy similarity
Binding sitei188 – 1881SubstrateBy similarity
Binding sitei190 – 1901SubstrateBy similarity
Metal bindingi218 – 2181MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_328870. Synthesis of GDP-mannose.
UniPathwayiUPA00126; UER00424.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphomannomutase 1 (EC:5.4.2.8)
Short name:
PMM 1
Gene namesi
Name:Pmm1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1353418. Pmm1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • neuronal cell body Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mice are viable and fertile and develop normally.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 262261Phosphomannomutase 1PRO_0000199693Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei158 – 1581N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiO35621.
PaxDbiO35621.
PRIDEiO35621.

PTM databases

PhosphoSiteiO35621.

Expressioni

Tissue specificityi

Present in brain, where it is restricted to neuronal cell bodies. Present at lower levels in pancreas, liver, lung, gonads, uterus, adrenal glands and pituitary (at protein level). Undetectable in intestine.1 Publication

Developmental stagei

Highly expressed at E17 in most organs (at protein level).1 Publication

Gene expression databases

BgeeiO35621.
CleanExiMM_PMM1.
ExpressionAtlasiO35621. baseline and differential.
GenevisibleiO35621. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023112.

Structurei

3D structure databases

ProteinModelPortaliO35621.
SMRiO35621. Positions 13-258.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the eukaryotic PMM family.Curated

Phylogenomic databases

eggNOGiCOG0561.
HOGENOMiHOG000181843.
HOVERGENiHBG009971.
InParanoidiO35621.
KOiK17497.
OMAiNDYEIYD.
OrthoDBiEOG773XH0.
PhylomeDBiO35621.
TreeFamiTF300874.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
InterProiIPR023214. HAD-like_dom.
IPR006379. HAD-SF_hydro_IIB.
IPR005002. PMM.
[Graphical view]
PANTHERiPTHR10466. PTHR10466. 1 hit.
PfamiPF03332. PMM. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01484. HAD-SF-IIB. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35621-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVAVEGARR KERILCLFDV DGTLTPARQK IDPEVSAFLQ KLRSRVQIGV
60 70 80 90 100
VGGSDYSKIA EQLGEGDEVI EKFDYVFAEN GTVQYKHGRL LSKQTIQNHL
110 120 130 140 150
GEELLQDLIN FCLSYMALLR LPKKRGTFIE FRNGMLNVSP IGRSCTLEER
160 170 180 190 200
IEFSELDKKE KIREKFVEAL KTEFAGKGLR FSRGGMISFD VFPEGWDKRY
210 220 230 240 250
CLDSLDEDSF DIIHFFGNET SPGGNDFEIY ADPRTVGHSV VSPQDTVQRC
260
RELFFPETAH EA
Length:262
Mass (Da):29,775
Last modified:January 1, 1998 - v1
Checksum:i2967154C0FF292B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF007267 mRNA. Translation: AAB62943.1.
CCDSiCCDS27678.1.
RefSeqiNP_038900.1. NM_013872.4.
UniGeneiMm.18939.

Genome annotation databases

EnsembliENSMUST00000023112; ENSMUSP00000023112; ENSMUSG00000022474.
GeneIDi29858.
KEGGimmu:29858.
UCSCiuc007wxt.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF007267 mRNA. Translation: AAB62943.1.
CCDSiCCDS27678.1.
RefSeqiNP_038900.1. NM_013872.4.
UniGeneiMm.18939.

3D structure databases

ProteinModelPortaliO35621.
SMRiO35621. Positions 13-258.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023112.

PTM databases

PhosphoSiteiO35621.

Proteomic databases

MaxQBiO35621.
PaxDbiO35621.
PRIDEiO35621.

Protocols and materials databases

DNASUi29858.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023112; ENSMUSP00000023112; ENSMUSG00000022474.
GeneIDi29858.
KEGGimmu:29858.
UCSCiuc007wxt.2. mouse.

Organism-specific databases

CTDi5372.
MGIiMGI:1353418. Pmm1.

Phylogenomic databases

eggNOGiCOG0561.
HOGENOMiHOG000181843.
HOVERGENiHBG009971.
InParanoidiO35621.
KOiK17497.
OMAiNDYEIYD.
OrthoDBiEOG773XH0.
PhylomeDBiO35621.
TreeFamiTF300874.

Enzyme and pathway databases

UniPathwayiUPA00126; UER00424.
ReactomeiREACT_328870. Synthesis of GDP-mannose.

Miscellaneous databases

ChiTaRSiPmm1. mouse.
NextBioi307058.
PROiO35621.
SOURCEiSearch...

Gene expression databases

BgeeiO35621.
CleanExiMM_PMM1.
ExpressionAtlasiO35621. baseline and differential.
GenevisibleiO35621. MM.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
InterProiIPR023214. HAD-like_dom.
IPR006379. HAD-SF_hydro_IIB.
IPR005002. PMM.
[Graphical view]
PANTHERiPTHR10466. PTHR10466. 1 hit.
PfamiPF03332. PMM. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01484. HAD-SF-IIB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Janoueix-Lerosey I., Goud B.
    Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The normal phenotype of Pmm1-deficient mice suggests that Pmm1 is not essential for normal mouse development."
    Cromphout K., Vleugels W., Heykants L., Schollen E., Keldermans L., Sciot R., D'Hooge R., De Deyn P.P., von Figura K., Hartmann D., Korner C., Matthijs G.
    Mol. Cell. Biol. 26:5621-5635(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
  3. "Mammalian phosphomannomutase PMM1 is the brain IMP-sensitive glucose-1,6-bisphosphatase."
    Veiga-da-Cunha M., Vleugels W., Maliekal P., Matthijs G., Van Schaftingen E.
    J. Biol. Chem. 283:33988-33993(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiPMM1_MOUSE
AccessioniPrimary (citable) accession number: O35621
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: January 1, 1998
Last modified: June 24, 2015
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.