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Protein

Zinc finger protein ZFPM1

Gene

Zfpm1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription regulator that plays an essential role in erythroid and megakaryocytic cell differentiation. Essential cofactor that acts via the formation of a heterodimer with transcription factors of the GATA family GATA1, GATA2 and GATA3. Such heterodimer can both activate or repress transcriptional activity, depending on the cell and promoter context. The heterodimer formed with GATA proteins is essential to activate expression of genes such as NFE2, ITGA2B, alpha- and beta-globin, while it represses expression of KLF1. May be involved in regulation of some genes in gonads. May also be involved in cardiac development, in a non-redundant way with ZFPM2/FOG2.7 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri249 – 282CCHC FOG-type 1PROSITE-ProRule annotationAdd BLAST34
Zinc fingeri303 – 327C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri333 – 355C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri361 – 384C2H2-type 3PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri584 – 617CCHC FOG-type 2PROSITE-ProRule annotationAdd BLAST34
Zinc fingeri690 – 723CCHC FOG-type 3PROSITE-ProRule annotationAdd BLAST34
Zinc fingeri830 – 863CCHC FOG-type 4PROSITE-ProRule annotationAdd BLAST34
Zinc fingeri868 – 891C2H2-type 4PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri957 – 990CCHC FOG-type 5PROSITE-ProRule annotationAdd BLAST34

GO - Molecular functioni

GO - Biological processi

  • atrial septum morphogenesis Source: BHF-UCL
  • atrioventricular valve morphogenesis Source: BHF-UCL
  • cardiac muscle tissue morphogenesis Source: BHF-UCL
  • definitive erythrocyte differentiation Source: MGI
  • embryonic hemopoiesis Source: BHF-UCL
  • erythrocyte differentiation Source: MGI
  • granulocyte differentiation Source: MGI
  • heart development Source: MGI
  • homeostasis of number of cells Source: MGI
  • megakaryocyte development Source: MGI
  • megakaryocyte differentiation Source: MGI
  • mitral valve formation Source: BHF-UCL
  • negative regulation of fat cell differentiation Source: UniProtKB
  • negative regulation of interleukin-4 biosynthetic process Source: MGI
  • negative regulation of mast cell differentiation Source: BHF-UCL
  • negative regulation of protein binding Source: BHF-UCL
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • outflow tract morphogenesis Source: BHF-UCL
  • platelet formation Source: MGI
  • positive regulation of interferon-gamma biosynthetic process Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • primitive erythrocyte differentiation Source: MGI
  • regulation of chemokine production Source: MGI
  • regulation of definitive erythrocyte differentiation Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
  • transcriptional activation by promoter-enhancer looping Source: BHF-UCL
  • tricuspid valve formation Source: BHF-UCL
  • ventricular septum morphogenesis Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-983231. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein ZFPM1
Alternative name(s):
Friend of GATA protein 1
Short name:
FOG-1
Short name:
Friend of GATA 1
Zinc finger protein multitype 1
Gene namesi
Name:Zfpm1
Synonyms:Fog, Fog1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1095400. Zfpm1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleus Source: MGI
  • transcriptional repressor complex Source: MGI
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi254V → A: Does not affect the interaction with GATA1. 1 Publication1
Mutagenesisi255F → A: Impairs interaction with GATA1. 1 Publication1
Mutagenesisi256P → A: Does not affect the interaction with GATA1. 1 Publication1
Mutagenesisi258K → A: Does not affect the interaction with GATA1. 1 Publication1
Mutagenesisi259D → A: Does not affect the interaction with GATA1. 1 Publication1
Mutagenesisi261G → A: Slightly affects the interaction with GATA1. 1 Publication1
Mutagenesisi262I → A: Impairs interaction with GATA1. 1 Publication1
Mutagenesisi263W → A: Does not affect the interaction with GATA1. 1 Publication1
Mutagenesisi265R → A: Slightly affects the interaction with GATA1. 1 Publication1
Mutagenesisi266S → A: Does not affect the interaction with GATA1. 1 Publication1
Mutagenesisi267E → A: Slightly affects the interaction with GATA1. 1 Publication1
Mutagenesisi268R → A: Does not affect the interaction with GATA1. 1 Publication1
Mutagenesisi269N → A: Impairs interaction with GATA1. 1 Publication1
Mutagenesisi271Q → A: Does not affect the interaction with GATA1. 1 Publication1
Mutagenesisi274L → A: Slightly affects the interaction with GATA1. 1 Publication1
Mutagenesisi275L → A: Does not affect the interaction with GATA1. 1 Publication1
Mutagenesisi276Y → A: Slightly affects the interaction with GATA1. 1 Publication1
Mutagenesisi277Y → A: Impairs interaction with GATA1. Strongly impairs interaction with GATA1; when associated with A-612; A-718 and/or A-985. 1 Publication1
Mutagenesisi280S → A: Does not affect the interaction with GATA1. 1 Publication1
Mutagenesisi281R → A: Does not affect the interaction with GATA1. 1 Publication1
Mutagenesisi334V → A: No effect on interaction with TACC3. 1 Publication1
Mutagenesisi336L → A: No effect on interaction with TACC3. 1 Publication1
Mutagenesisi339L → A: No effect on interaction with TACC3. 1 Publication1
Mutagenesisi340S → A: No effect on interaction with TACC3. 1 Publication1
Mutagenesisi343T → A: Impairs interaction with TACC3. 1 Publication1
Mutagenesisi344T → A: Abolishes interaction with TACC3. 1 Publication1
Mutagenesisi345K → A: No effect on interaction with TACC3. 1 Publication1
Mutagenesisi346A → D: No effect on interaction with TACC3. 1 Publication1
Mutagenesisi347N → A: Abolishes interaction with TACC3. 1 Publication1
Mutagenesisi349E → A: No effect on interaction with TACC3. 1 Publication1
Mutagenesisi350R → A: Abolishes interaction with TACC3. 1 Publication1
Mutagenesisi352L → A: No effect on interaction with TACC3. 1 Publication1
Mutagenesisi353K → A: No effect on interaction with TACC3. 1 Publication1
Mutagenesisi354V → A: Abolishes interaction with TACC3. 1 Publication1
Mutagenesisi356T → A: No effect on interaction with TACC3. 1 Publication1
Mutagenesisi357D → A: No effect on interaction with TACC3. 1 Publication1
Mutagenesisi612Y → A: Impairs interaction with GATA1. Strongly impairs interaction with GATA1; when associated with A-277; A-718 and/or A-985. 1 Publication1
Mutagenesisi698C → A: Abolishes interaction with GATA1. 1 Publication1
Mutagenesisi706S → R: Able to partially restore the interaction with the G-205 GATA1 mutant, which is usually unable to interact with ZFPM1. 1 Publication1
Mutagenesisi718Y → A: Impairs interaction with GATA1. Strongly impairs interaction with GATA1; when associated with A-277; A-612 and/or A-985. 1 Publication1
Mutagenesisi719C → A: Abolishes interaction with GATA1. 1 Publication1
Mutagenesisi719C → H: Transforms the C2HC-type zinc finger into a C2H2-type, leading to abolition of interaction with GATA1. 1 Publication1
Mutagenesisi811 – 814PIDL → AIAA: Abolishes interaction with CTBP2. 1 Publication4
Mutagenesisi813 – 814DL → AS: Abolishes interaction with CTBP2; it however does not abolish the corepressor activity in erythropoiesis. 1 Publication2
Mutagenesisi985Y → A: Slightly affects the interaction with GATA1. Strongly impairs interaction with GATA1; when associated with A-277; A-612 and/or A-718. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002210421 – 995Zinc finger protein ZFPM1Add BLAST995

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei99PhosphoserineBy similarity1
Modified residuei143PhosphoserineCombined sources1
Modified residuei286PhosphoserineCombined sources1
Modified residuei397PhosphoserineBy similarity1
Modified residuei497PhosphoserineCombined sources1
Modified residuei500PhosphoserineCombined sources1
Modified residuei651PhosphoserineBy similarity1
Modified residuei684PhosphoserineBy similarity1
Modified residuei803PhosphoserineBy similarity1
Modified residuei822PhosphoserineCombined sources1
Modified residuei925PhosphoserineCombined sources1
Modified residuei927PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO35615.
PaxDbiO35615.
PeptideAtlasiO35615.
PRIDEiO35615.

PTM databases

iPTMnetiO35615.
PhosphoSitePlusiO35615.

Expressioni

Tissue specificityi

Mainly expressed in hematopoietic tissues. Expressed in the spleen, a primary site of hematopoiesis in the adult mouse, as well as in the liver and testis, but not in the heart, brain, lung, kidney, or skeletal muscle. Among hematopoietic cell lines, it is strongly expressed in erythroid and megakaryocytic cell lines. Expressed at low level in several lymphoid and early myeloid cell lines. Not expressed in mast cell and macrophage lines. Expressed in the heart, where it colocalizes with GATA4, GATA5 and GATA6.2 Publications

Developmental stagei

First expressed in two extraembryonic mesodermal derivatives, the yolk sac and the allantois in E8.5 embryos. Localized to the embryonic red blood cells within the yolk sac blood islands.1 Publication

Gene expression databases

BgeeiENSMUSG00000049577.
CleanExiMM_ZFPM1.
ExpressionAtlasiO35615. baseline and differential.
GenevisibleiO35615. MM.

Interactioni

Subunit structurei

Interacts with the N-terminal zinc-finger of GATA1, GATA2 and GATA3. Interacts with corepressor CTBP2; this interaction is however not essential for corepressor activity in erythropoiesis. Interacts with TACC3.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Tacc3Q9JJ117EBI-4394596,EBI-2553611

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204687. 11 interactors.
DIPiDIP-48414N.
IntActiO35615. 3 interactors.
MINTiMINT-6613139.
STRINGi10090.ENSMUSP00000058037.

Structurei

Secondary structure

1995
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi107 – 112Combined sources6
Beta strandi115 – 122Combined sources8
Turni144 – 146Combined sources3
Helixi162 – 164Combined sources3
Beta strandi168 – 182Combined sources15
Beta strandi185 – 190Combined sources6
Beta strandi201 – 204Combined sources4
Beta strandi336 – 338Combined sources3
Beta strandi341 – 344Combined sources4
Helixi345 – 352Combined sources8
Helixi353 – 355Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SRKNMR-A328-360[»]
2MPLNMR-A100-226[»]
ProteinModelPortaliO35615.
SMRiO35615.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO35615.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni343 – 354Interaction with TACC31 PublicationAdd BLAST12
Regioni811 – 817Interaction with CTBP27

Domaini

The CCHC FOG-type zinc fingers 1, 2, 3 and 5 bind directly to GATA-type zinc fingers. The Tyr residue adjacent to the last Cys of the CCHC FOG-type zinc finger is essential for the interaction with GATA-type zinc fingers.

Sequence similaritiesi

Belongs to the FOG (Friend of GATA) family.PROSITE-ProRule annotation
Contains 4 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 5 CCHC FOG-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri249 – 282CCHC FOG-type 1PROSITE-ProRule annotationAdd BLAST34
Zinc fingeri303 – 327C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri333 – 355C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri361 – 384C2H2-type 3PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri584 – 617CCHC FOG-type 2PROSITE-ProRule annotationAdd BLAST34
Zinc fingeri690 – 723CCHC FOG-type 3PROSITE-ProRule annotationAdd BLAST34
Zinc fingeri830 – 863CCHC FOG-type 4PROSITE-ProRule annotationAdd BLAST34
Zinc fingeri868 – 891C2H2-type 4PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri957 – 990CCHC FOG-type 5PROSITE-ProRule annotationAdd BLAST34

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00530000063823.
HOGENOMiHOG000112626.
HOVERGENiHBG101018.
InParanoidiO35615.
KOiK17441.
OMAiYSCPAAP.
OrthoDBiEOG091G00RC.
PhylomeDBiO35615.
TreeFamiTF331342.

Family and domain databases

Gene3Di3.30.160.60. 2 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 9 hits.
[Graphical view]
PROSITEiPS51810. ZF_CCHC_FOG. 5 hits.
PS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35615-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRRKQSNPR QIKRSLRDME AGEEAKAMDS SPKEQEAPDP EAPAIEEPPS
60 70 80 90 100
PPREDVSPPA VPAPPESPED PEDMEGQELE MRPQDEEKEE KEEEAAMASP
110 120 130 140 150
WSGPEELELA LQDGQRCVRA RLSLTEGLSW GPFYGSIQTR ALSPEREEPG
160 170 180 190 200
PAVTLMVDES CWLRMLPQVL TEEAANSEIY RKDDALWCRV TKVVPSGGLL
210 220 230 240 250
YVRLVTEPHG APRHPVQEPV EPGGLAPVHT DIQLLPQQAG MASILATAVI
260 270 280 290 300
NKDVFPCKDC GIWYRSERNL QAHLLYYCAS RQRAGSPVSA TEEKPKETYP
310 320 330 340 350
NERVCPFPQC RKSCPSASSL EIHMRSHSGE RPFVCLICLS AFTTKANCER
360 370 380 390 400
HLKVHTDTLS GVCHNCGFIS TTRDILYSHL VTNHMVCQPG SKGEIYSPGA
410 420 430 440 450
GHPAAKLPPD SLAGFQQHSL MHSPLVPADK APTPSSGLDS KAEVTNGETR
460 470 480 490 500
VPPQNGGSSE SPAAPRTIKV EAAEEPEATR ASGPGEPGPQ APSRTPSPHS
510 520 530 540 550
PNPVRVKTEL SSPTPGSSPG PGELTMAGTL FLPQYVFSPD AGTTTVPTAP
560 570 580 590 600
QASEILAKMS ELVHNRLQQG AGSSGAAGTP TGLFSGTKGA TCFECEITFN
610 620 630 640 650
NINNFYVHKR LYCSGRRAPE DPPTVRRPKA ATGPARAPAG AAAEPDPSRS
660 670 680 690 700
SPGPGPREEE ASGTTTPEAE AAGRGSEGSQ SPGSSVDDAE DDPSRTLCEA
710 720 730 740 750
CNIRFSRHET YTVHKRYYCA SRHDPPPRRP PAPTTAPGPA APALTAPPVR
760 770 780 790 800
TRRRRKLYEL PAAGAPPPAA GPAPVPVVPS PTAELPSSPR PGSASAGPAP
810 820 830 840 850
ALSPSPVPDG PIDLSKRPRR QSPDAPTALP ALADYHECTA CRVSFHSLEA
860 870 880 890 900
YLAHKKYSCP AAPLRTTALC PYCPPNGRVR GDLVEHLRQA HGLQVAKPAA
910 920 930 940 950
SPGAEPRTPA ERAPRDSPDG RAPRSPSPAP ENTPSDPADQ GARTPSKGPP
960 970 980 990
APAPAPGGGG GHRYCRLCNI RFSSLSTFIA HKKYYCSSHA AEHVK
Length:995
Mass (Da):105,984
Last modified:January 1, 1998 - v1
Checksum:i293255B28151ECB8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF006492 mRNA. Translation: AAC53292.1.
CCDSiCCDS22733.1.
RefSeqiNP_033595.1. NM_009569.3.
XP_006530914.1. XM_006530851.3.
UniGeneiMm.3105.
Mm.390068.

Genome annotation databases

EnsembliENSMUST00000054052; ENSMUSP00000058037; ENSMUSG00000049577.
GeneIDi22761.
KEGGimmu:22761.
UCSCiuc009nsj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF006492 mRNA. Translation: AAC53292.1.
CCDSiCCDS22733.1.
RefSeqiNP_033595.1. NM_009569.3.
XP_006530914.1. XM_006530851.3.
UniGeneiMm.3105.
Mm.390068.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SRKNMR-A328-360[»]
2MPLNMR-A100-226[»]
ProteinModelPortaliO35615.
SMRiO35615.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204687. 11 interactors.
DIPiDIP-48414N.
IntActiO35615. 3 interactors.
MINTiMINT-6613139.
STRINGi10090.ENSMUSP00000058037.

PTM databases

iPTMnetiO35615.
PhosphoSitePlusiO35615.

Proteomic databases

MaxQBiO35615.
PaxDbiO35615.
PeptideAtlasiO35615.
PRIDEiO35615.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000054052; ENSMUSP00000058037; ENSMUSG00000049577.
GeneIDi22761.
KEGGimmu:22761.
UCSCiuc009nsj.1. mouse.

Organism-specific databases

CTDi161882.
MGIiMGI:1095400. Zfpm1.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00530000063823.
HOGENOMiHOG000112626.
HOVERGENiHBG101018.
InParanoidiO35615.
KOiK17441.
OMAiYSCPAAP.
OrthoDBiEOG091G00RC.
PhylomeDBiO35615.
TreeFamiTF331342.

Enzyme and pathway databases

ReactomeiR-MMU-983231. Factors involved in megakaryocyte development and platelet production.

Miscellaneous databases

EvolutionaryTraceiO35615.
PROiO35615.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000049577.
CleanExiMM_ZFPM1.
ExpressionAtlasiO35615. baseline and differential.
GenevisibleiO35615. MM.

Family and domain databases

Gene3Di3.30.160.60. 2 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 9 hits.
[Graphical view]
PROSITEiPS51810. ZF_CCHC_FOG. 5 hits.
PS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFOG1_MOUSE
AccessioniPrimary (citable) accession number: O35615
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.