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O35608

- ANGP2_MOUSE

UniProt

O35608 - ANGP2_MOUSE

Protein

Angiopoietin-2

Gene

Angpt2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 2 (26 Apr 2004)
      Previous versions | rss
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    Functioni

    Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi429 – 4291CalciumBy similarity
    Metal bindingi431 – 4311CalciumBy similarity
    Metal bindingi433 – 4331Calcium; via carbonyl oxygenBy similarity
    Metal bindingi435 – 4351Calcium; via carbonyl oxygenBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: MGI
    3. vascular endothelial growth factor receptor binding Source: MGI

    GO - Biological processi

    1. angiogenesis Source: MGI
    2. blood vessel morphogenesis Source: MGI
    3. blood vessel remodeling Source: DFLAT
    4. cellular response to growth factor stimulus Source: Ensembl
    5. endoderm development Source: MGI
    6. germ cell development Source: Ensembl
    7. glomerulus vasculature development Source: Ensembl
    8. hemopoiesis Source: DFLAT
    9. maternal process involved in female pregnancy Source: Ensembl
    10. negative regulation of angiogenesis Source: MGI
    11. negative regulation of blood vessel endothelial cell migration Source: Ensembl
    12. negative regulation of cell-substrate adhesion Source: MGI
    13. negative regulation of positive chemotaxis Source: Ensembl
    14. organ regeneration Source: Ensembl
    15. positive regulation of angiogenesis Source: Ensembl
    16. regulation of angiogenesis Source: DFLAT
    17. response to activity Source: Ensembl
    18. response to glucose Source: Ensembl
    19. response to hypoxia Source: Ensembl
    20. response to mechanical stimulus Source: Ensembl
    21. response to organic cyclic compound Source: Ensembl
    22. response to radiation Source: Ensembl
    23. Tie signaling pathway Source: DFLAT
    24. transmembrane receptor protein tyrosine kinase signaling pathway Source: MGI

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Angiogenesis, Differentiation

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_211860. Tie2 Signaling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Angiopoietin-2
    Short name:
    ANG-2
    Gene namesi
    Name:Angpt2
    Synonyms:Agpt2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:1202890. Angpt2.

    Subcellular locationi

    GO - Cellular componenti

    1. cell projection Source: Ensembl
    2. extracellular space Source: Ensembl
    3. nucleus Source: Ensembl
    4. plasma membrane Source: Ensembl

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 496478Angiopoietin-2PRO_0000009114Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi89 – 891N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi119 – 1191N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi133 – 1331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi151 – 1511N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi240 – 2401N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi284 ↔ 313PROSITE-ProRule annotation
    Glycosylationi304 – 3041N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi433 ↔ 435PROSITE-ProRule annotation
    Disulfide bondi437 ↔ 450PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiO35608.

    PTM databases

    PhosphoSiteiO35608.

    Expressioni

    Tissue specificityi

    Expressed only at sites of vascular remodeling.

    Gene expression databases

    ArrayExpressiO35608.
    BgeeiO35608.
    CleanExiMM_ANGPT2.
    GenevestigatoriO35608.

    Interactioni

    Subunit structurei

    Interacts with TEK/TIE2, competing for the same binding site as ANGPT1.By similarity

    Protein-protein interaction databases

    DIPiDIP-6049N.
    IntActiO35608. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliO35608.
    SMRiO35608. Positions 148-495.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini275 – 495221Fibrinogen C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili159 – 25698Sequence AnalysisAdd
    BLAST

    Domaini

    The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor.By similarity

    Sequence similaritiesi

    Contains 1 fibrinogen C-terminal domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Signal

    Phylogenomic databases

    eggNOGiNOG298026.
    GeneTreeiENSGT00720000108441.
    HOGENOMiHOG000037128.
    HOVERGENiHBG001644.
    InParanoidiO35608.
    KOiK05466.
    OMAiIKAYCDM.
    OrthoDBiEOG7X9G60.
    PhylomeDBiO35608.
    TreeFamiTF336658.

    Family and domain databases

    Gene3Di3.90.215.10. 1 hit.
    4.10.530.10. 1 hit.
    InterProiIPR028844. Ang-2.
    IPR014716. Fibrinogen_a/b/g_C_1.
    IPR014715. Fibrinogen_a/b/g_C_2.
    IPR002181. Fibrinogen_a/b/g_C_dom.
    IPR020837. Fibrinogen_CS.
    [Graphical view]
    PANTHERiPTHR19143:SF199. PTHR19143:SF199. 1 hit.
    PfamiPF00147. Fibrinogen_C. 1 hit.
    [Graphical view]
    SMARTiSM00186. FBG. 1 hit.
    [Graphical view]
    SUPFAMiSSF56496. SSF56496. 1 hit.
    PROSITEiPS00514. FIBRINOGEN_C_1. 1 hit.
    PS51406. FIBRINOGEN_C_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O35608-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWQIIFLTFG WDLVLASAYS NFRKSVDSTG RRQYQVQNGP CSYTFLLPET    50
    DSCRSSSSPY MSNAVQRDAP LDYDDSVQRL QVLENILENN TQWLMKLENY 100
    IQDNMKKEMV EIQQNVVQNQ TAVMIEIGTS LLNQTAAQTR KLTDVEAQVL 150
    NQTTRLELQL LQHSISTNKL EKQILDQTSE INKLQNKNSF LEQKVLDMEG 200
    KHSEQLQSMK EQKDELQVLV SKQSSVIDEL EKKLVTATVN NSLLQKQQHD 250
    LMETVNSLLT MMSSPNSKSS VAIRKEEQTT FRDCAEIFKS GLTTSGIYTL 300
    TFPNSTEEIK AYCDMDVGGG GWTVIQHRED GSVDFQRTWK EYKEGFGSPL 350
    GEYWLGNEFV SQLTGQHRYV LKIQLKDWEG NEAHSLYDHF YLAGEESNYR 400
    IHLTGLTGTA GKISSISQPG SDFSTKDSDN DKCICKCSQM LSGGWWFDAC 450
    GPSNLNGQYY PQKQNTNKFN GIKWYYWKGS GYSLKATTMM IRPADF 496
    Length:496
    Mass (Da):56,576
    Last modified:April 26, 2004 - v2
    Checksum:iE7563B498A0EF331
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti348 – 3481S → N in AAB63189. (PubMed:9204896)Curated
    Sequence conflicti411 – 4111G → A in AAB63189. (PubMed:9204896)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF004326 mRNA. Translation: AAB63189.1.
    AK019860 mRNA. Translation: BAB31887.1.
    AK048622 mRNA. Translation: BAC33396.1.
    AK156132 mRNA. Translation: BAE33599.1.
    BC027216 mRNA. Translation: AAH27216.1.
    CCDSiCCDS22125.1.
    RefSeqiNP_031452.2. NM_007426.4.
    UniGeneiMm.439874.

    Genome annotation databases

    EnsembliENSMUST00000033846; ENSMUSP00000033846; ENSMUSG00000031465.
    GeneIDi11601.
    KEGGimmu:11601.
    UCSCiuc009kzu.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF004326 mRNA. Translation: AAB63189.1 .
    AK019860 mRNA. Translation: BAB31887.1 .
    AK048622 mRNA. Translation: BAC33396.1 .
    AK156132 mRNA. Translation: BAE33599.1 .
    BC027216 mRNA. Translation: AAH27216.1 .
    CCDSi CCDS22125.1.
    RefSeqi NP_031452.2. NM_007426.4.
    UniGenei Mm.439874.

    3D structure databases

    ProteinModelPortali O35608.
    SMRi O35608. Positions 148-495.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-6049N.
    IntActi O35608. 1 interaction.

    PTM databases

    PhosphoSitei O35608.

    Proteomic databases

    PRIDEi O35608.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000033846 ; ENSMUSP00000033846 ; ENSMUSG00000031465 .
    GeneIDi 11601.
    KEGGi mmu:11601.
    UCSCi uc009kzu.1. mouse.

    Organism-specific databases

    CTDi 285.
    MGIi MGI:1202890. Angpt2.

    Phylogenomic databases

    eggNOGi NOG298026.
    GeneTreei ENSGT00720000108441.
    HOGENOMi HOG000037128.
    HOVERGENi HBG001644.
    InParanoidi O35608.
    KOi K05466.
    OMAi IKAYCDM.
    OrthoDBi EOG7X9G60.
    PhylomeDBi O35608.
    TreeFami TF336658.

    Enzyme and pathway databases

    Reactomei REACT_211860. Tie2 Signaling.

    Miscellaneous databases

    NextBioi 279120.
    PROi O35608.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O35608.
    Bgeei O35608.
    CleanExi MM_ANGPT2.
    Genevestigatori O35608.

    Family and domain databases

    Gene3Di 3.90.215.10. 1 hit.
    4.10.530.10. 1 hit.
    InterProi IPR028844. Ang-2.
    IPR014716. Fibrinogen_a/b/g_C_1.
    IPR014715. Fibrinogen_a/b/g_C_2.
    IPR002181. Fibrinogen_a/b/g_C_dom.
    IPR020837. Fibrinogen_CS.
    [Graphical view ]
    PANTHERi PTHR19143:SF199. PTHR19143:SF199. 1 hit.
    Pfami PF00147. Fibrinogen_C. 1 hit.
    [Graphical view ]
    SMARTi SM00186. FBG. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56496. SSF56496. 1 hit.
    PROSITEi PS00514. FIBRINOGEN_C_1. 1 hit.
    PS51406. FIBRINOGEN_C_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Uterus.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Head, Ovary, Spleen and Uterus.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiANGP2_MOUSE
    AccessioniPrimary (citable) accession number: O35608
    Secondary accession number(s): Q3U1A1, Q9D2D2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: April 26, 2004
    Last modified: October 1, 2014
    This is version 125 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3