O35608 (ANGP2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Angiopoietin-2
Short name=ANG-2

Gene names

Name:	Angpt2
Synonyms:	Agpt2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal By similarity.
Subunit structure	Interacts with TEK/TIE2, competing for the same binding site as ANGPT1 By similarity.
Subcellular location	Secreted.
Tissue specificity	Expressed only at sites of vascular remodeling.
Domain	The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor By similarity.
Sequence similarities	Contains 1 fibrinogen C-terminal domain.

Ontologies

Keywords
Biological process	Angiogenesis Differentiation
Cellular component	Secreted
Domain	Coiled coil Signal
Ligand	Calcium Metal-binding
Molecular function	Developmental protein
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Tie receptor signaling pathway Traceable author statement PubMed 20299672. Source: DFLAT angiogenesis Inferred from genetic interaction PubMed 16778080. Source: MGI blood vessel remodeling Traceable author statement PubMed 20299672. Source: DFLAT cellular response to growth factor stimulus Inferred from electronic annotation. Source: Compara endoderm development Traceable author statement PubMed 10051567. Source: MGI germ cell development Inferred from electronic annotation. Source: Compara glomerulus vasculature development Inferred from electronic annotation. Source: Compara hemopoiesis Traceable author statement PubMed 20299672. Source: DFLAT maternal process involved in female pregnancy Inferred from electronic annotation. Source: Compara negative regulation of angiogenesis Inferred from direct assay Ref.1. Source: MGI negative regulation of blood vessel endothelial cell migration Inferred from electronic annotation. Source: Compara negative regulation of cell-substrate adhesion Inferred from direct assay PubMed 9846489. Source: MGI negative regulation of positive chemotaxis Inferred from electronic annotation. Source: Compara organ regeneration Inferred from electronic annotation. Source: Compara positive regulation of angiogenesis Inferred from electronic annotation. Source: Compara response to activity Inferred from electronic annotation. Source: Compara response to glucose stimulus Inferred from electronic annotation. Source: Compara response to hypoxia Inferred from electronic annotation. Source: Compara response to mechanical stimulus Inferred from electronic annotation. Source: Compara response to organic cyclic compound Inferred from electronic annotation. Source: Compara response to radiation Inferred from electronic annotation. Source: Compara
Cellular_component	cell projection Inferred from electronic annotation. Source: Compara extracellular space Inferred from electronic annotation. Source: Compara nucleus Inferred from electronic annotation. Source: Compara plasma membrane Inferred from electronic annotation. Source: Compara
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW vascular endothelial growth factor receptor binding Traceable author statement PubMed 10051567. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 496	478	Angiopoietin-2		PRO_0000009114
Regions
	Domain	275 – 495	221	Fibrinogen C-terminal
	Coiled coil	159 – 256	98	Potential
Sites
	Metal binding	429	1	Calcium By similarity
	Metal binding	431	1	Calcium By similarity
	Metal binding	433	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	435	1	Calcium; via carbonyl oxygen By similarity
Amino acid modifications
	Glycosylation	89	1	N-linked (GlcNAc...) Potential
	Glycosylation	119	1	N-linked (GlcNAc...) Potential
	Glycosylation	133	1	N-linked (GlcNAc...) Potential
	Glycosylation	151	1	N-linked (GlcNAc...) Potential
	Glycosylation	240	1	N-linked (GlcNAc...) Potential
	Glycosylation	304	1	N-linked (GlcNAc...) Potential
	Disulfide bond	284 ↔ 313		By similarity
	Disulfide bond	433 ↔ 435		By similarity
	Disulfide bond	437 ↔ 450		By similarity
Experimental info
	Sequence conflict	348	1	S → N in AAB63189. Ref.1
	Sequence conflict	411	1	G → A in AAB63189. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O35608 [UniParc]. Last modified April 26, 2004. Version 2. Checksum: E7563B498A0EF331	FASTA	496	56,576
	10 20 30 40 50 60 MWQIIFLTFG WDLVLASAYS NFRKSVDSTG RRQYQVQNGP CSYTFLLPET DSCRSSSSPY 70 80 90 100 110 120 MSNAVQRDAP LDYDDSVQRL QVLENILENN TQWLMKLENY IQDNMKKEMV EIQQNVVQNQ 130 140 150 160 170 180 TAVMIEIGTS LLNQTAAQTR KLTDVEAQVL NQTTRLELQL LQHSISTNKL EKQILDQTSE 190 200 210 220 230 240 INKLQNKNSF LEQKVLDMEG KHSEQLQSMK EQKDELQVLV SKQSSVIDEL EKKLVTATVN 250 260 270 280 290 300 NSLLQKQQHD LMETVNSLLT MMSSPNSKSS VAIRKEEQTT FRDCAEIFKS GLTTSGIYTL 310 320 330 340 350 360 TFPNSTEEIK AYCDMDVGGG GWTVIQHRED GSVDFQRTWK EYKEGFGSPL GEYWLGNEFV 370 380 390 400 410 420 SQLTGQHRYV LKIQLKDWEG NEAHSLYDHF YLAGEESNYR IHLTGLTGTA GKISSISQPG 430 440 450 460 470 480 SDFSTKDSDN DKCICKCSQM LSGGWWFDAC GPSNLNGQYY PQKQNTNKFN GIKWYYWKGS 490 GYSLKATTMM IRPADF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Angiopoietin-2, a natural antagonist for Tie2 that disrupts in vivo angiogenesis." Maisonpierre P.C., Suri C., Jones P.F., Bartunkova S., Wiegand S.J., Radziejewski C., Compton D.L., McClain J., Aldrich T.H., Papadopoulos N., Daly T.J., Davis S., Sato T.N., Yancopoulos G.D. Science 277:55-60(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Uterus.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Head, Ovary, Spleen and Uterus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF004326 mRNA. Translation: AAB63189.1. AK019860 mRNA. Translation: BAB31887.1. AK048622 mRNA. Translation: BAC33396.1. AK156132 mRNA. Translation: BAE33599.1. BC027216 mRNA. Translation: AAH27216.1.
IPI	IPI00132602.
RefSeq	NP_031452.2. NM_007426.4.
UniGene	Mm.439874.
3D structure databases
ProteinModelPortal	O35608.
SMR	O35608. Positions 148-495.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6049N.
IntAct	O35608. 1 interaction.
PTM databases
PhosphoSite	O35608.
Proteomic databases
PRIDE	O35608.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000033846; ENSMUSP00000033846; ENSMUSG00000031465.
GeneID	11601.
KEGG	mmu:11601.
UCSC	uc009kzu.1. mouse.
Organism-specific databases
CTD	285.
MGI	MGI:1202890. Angpt2.
Phylogenomic databases
eggNOG	NOG298026.
GeneTree	ENSGT00700000104380.
HOGENOM	HOG000037128.
HOVERGEN	HBG001644.
InParanoid	O35608.
KO	K05466.
OMA	IKAYCDM.
OrthoDB	EOG4D7Z5N.
Gene expression databases
ArrayExpress	O35608.
Bgee	O35608.
CleanEx	MM_ANGPT2.
Genevestigator	O35608.
GermOnline	ENSMUSG00000031465. Mus musculus.
Family and domain databases
Gene3D	3.90.215.10. 1 hit. 4.10.530.10. 1 hit.
InterPro	IPR014716. Fibrinogen_a/b/g_C_1. IPR014715. Fibrinogen_a/b/g_C_2. IPR002181. Fibrinogen_a/b/g_C_dom. IPR020837. Fibrinogen_CS. [Graphical view]
Pfam	PF00147. Fibrinogen_C. 1 hit. [Graphical view]
SMART	SM00186. FBG. 1 hit. [Graphical view]
SUPFAM	SSF56496. Fibrinogen_a/b/g_C. 1 hit.
PROSITE	PS00514. FIBRINOGEN_C_1. 1 hit. PS51406. FIBRINOGEN_C_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	279120.
SOURCE	Search...

Entry information

Entry name

ANGP2_MOUSE

Accession

Primary (citable) accession number: O35608
Secondary accession number(s): Q3U1A1, Q9D2D2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	April 26, 2004
Last modified:	May 29, 2013
This is version 111 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program