Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O35601

- FYB_MOUSE

UniProt

O35601 - FYB_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

FYN-binding protein

Gene

Fyb

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May play a role in linking T-cell signaling to remodeling of the actin cytoskeleton (By similarity). Acts as an adapter protein of the FYN and LCP2 signaling cascades in T-cells. Modulates the expression of interleukin-2 (IL-2). Involved in platelet activation. Prevents the degradation of SKAP1 and SKAP2.By similarity3 Publications

Enzyme and pathway databases

ReactomeiREACT_215628. Signal regulatory protein (SIRP) family interactions.
REACT_225768. Generation of second messenger molecules.

Names & Taxonomyi

Protein namesi
Recommended name:
FYN-binding protein
Alternative name(s):
Adhesion and degranulation promoting adaptor protein
Short name:
ADAP
FYB-120/130
Short name:
p120/p130
FYN-T-binding protein
SLAP-130
SLP-76-associated phosphoprotein
Gene namesi
Name:Fyb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:1346327. Fyb.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Slight defects in platelet function.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 819818FYN-binding proteinPRO_0000087397Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei3 – 31N6-acetyllysineBy similarity
Modified residuei28 – 281Phosphoserine1 Publication
Modified residuei46 – 461PhosphoserineBy similarity
Modified residuei445 – 4451PhosphoserineBy similarity
Modified residuei559 – 5591Phosphotyrosine1 Publication
Modified residuei561 – 5611Phosphoserine1 Publication
Modified residuei687 – 6871PhosphotyrosineBy similarity

Post-translational modificationi

T-cell receptor ligation leads to increased tyrosine phosphorylation.2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiO35601.
PaxDbiO35601.
PRIDEiO35601.

PTM databases

PhosphoSiteiO35601.

Miscellaneous databases

PMAP-CutDBO35601.

Expressioni

Tissue specificityi

Expressed in hematopoietic tissues such as myeloid and T-cells, spleen and thymus. Not expressed in B-cells, nor in non-lymphoid tissues. FYB-130 is preferentially expressed in mature T-cells compared to FYB-120, whereas thymocytes showed a greater relative amount of FYB-120.

Gene expression databases

BgeeiO35601.
CleanExiMM_FYB.
ExpressionAtlasiO35601. baseline and differential.
GenevestigatoriO35601.

Interactioni

Subunit structurei

Interacts with FYN, LCP2, SKAP1 and SKAP2. Part of a complex consisting of SKAP2, FYB and PTPNS1. Part of a complex consisting of SKAP2, FYB and LILRB3. Interacts with FASLG. Interacts with EVL (By similarity).By similarity

Protein-protein interaction databases

BioGridi204772. 3 interactions.
DIPiDIP-41350N.
IntActiO35601. 2 interactions.
MINTiMINT-244320.
STRINGi10090.ENSMUSP00000087947.

Structurei

3D structure databases

ProteinModelPortaliO35601.
SMRiO35601. Positions 494-567, 725-801.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini699 – 75658SH3Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili448 – 49548Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi479 – 49315Nuclear localization signalSequence AnalysisAdd
BLAST
Motifi584 – 5874SH2-binding; to LCP2
Motifi615 – 6184SH2-binding; to FYN
Motifi710 – 73627Nuclear localization signalSequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi343 – 3486Poly-Pro
Compositional biasi381 – 3855Poly-Pro
Compositional biasi606 – 6094Poly-Pro
Compositional biasi622 – 6254Poly-Asp
Compositional biasi696 – 6994Poly-Pro

Sequence similaritiesi

Contains 1 SH3 domain.Curated

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiNOG18948.
GeneTreeiENSGT00530000063460.
HOGENOMiHOG000008686.
HOVERGENiHBG005774.
InParanoidiO35601.
KOiK17698.
OMAiACCDVKG.
OrthoDBiEOG70W3CP.
PhylomeDBiO35601.
TreeFamiTF337003.

Family and domain databases

InterProiIPR029294. hSH3.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF14603. hSH3. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform FYB-130 (identifier: O35601-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAKFNTGSNP TEEAATSSRP FKVAGQSSPS GIQSRKNLFD NQGNASPPAG
60 70 80 90 100
PSSMPKFGTT KPPLAAKPTY EEKPEKEPKP PFLKPTGGSP RFGTQPNSVS
110 120 130 140 150
RDPEVKVGFL KPVSPKPTSL TKEDSKPVVL RPPGNKLHNL NQESDLKTPG
160 170 180 190 200
PKPGPAPPVP ENELKPGFSK VAGAKSKFMP AAQDTDSKPR FPRHTFGQKP
210 220 230 240 250
SLSTEDSQEE NTSKNVPVQK GSPVQLGAKS KGAPFKPPKE DPEDKDHGAP
260 270 280 290 300
SSPFPGVVLK PAASRGSPGL SKNFEEKKED RKTDLAKNIF LNKLNQEEPA
310 320 330 340 350
RFPKAPSKLT AGTPWGQSQE KEGDKNSATP KQKALPPLSV LGPPPPKPNR
360 370 380 390 400
PPNVDLTRFR KADSANSATK SQTPYSTTSL PPPPPTHPAS QPPLPASHPA
410 420 430 440 450
HPPVPSLPPR NIKPPLDLKH PINDENQDGV MHSDGTGNLE EEQESEGETY
460 470 480 490 500
EDIDSSKERD KKREKEEKKR LELERKEQKE REKKEQELKK KFKLTGPIQV
510 520 530 540 550
IHHAKACCDV KGGKNELSFK QGEDIEIIRI TDNPEGKWLG RTARGSYGYI
560 570 580 590 600
KTTAVEIDYD SLKRKKNSLN AVPPRLVEDD QDVYDDVAEQ DAPNSHGQSG
610 620 630 640 650
SGGMFPPPPT DDEIYDGIEE EDDDDGSVPQ VDEKTNAWSW GILKMLKGKD
660 670 680 690 700
DRKKSIREKP KVSESDNNEG SSLPSQHKQL DVGEEVYDDV DASDFPPPPA
710 720 730 740 750
EMSQGMSVGR AKTEEKDPKK LKKQEKEEKD LRKKFKYDGE IRVLYSTKVA
760 770 780 790 800
SSLTSKKWGA RDLQIKPGES LEVIQSTDDT KVLCRNEEGK YGYVLRSYLV
810
DNDGEIYDDI ADGCIYDND
Length:819
Mass (Da):90,055
Last modified:December 1, 2000 - v2
Checksum:i6222CC7EF1CA2BBD
GO
Isoform FYB-120 (identifier: O35601-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     627-672: Missing.

Show »
Length:773
Mass (Da):84,870
Checksum:iA3EC5C9AA7124B69
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei627 – 67246Missing in isoform FYB-120. 1 PublicationVSP_004261Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF001863 mRNA. Translation: AAB62227.1.
AF061744 mRNA. Translation: AAD03267.1.
CCDSiCCDS37031.1. [O35601-1]
RefSeqiNP_001265198.1. NM_001278269.1. [O35601-2]
NP_035945.1. NM_011815.5. [O35601-1]
UniGeneiMm.170905.

Genome annotation databases

EnsembliENSMUST00000090461; ENSMUSP00000087947; ENSMUSG00000022148. [O35601-1]
GeneIDi23880.
KEGGimmu:23880.
UCSCiuc007vdi.1. mouse. [O35601-1]
uc007vdk.1. mouse. [O35601-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF001863 mRNA. Translation: AAB62227.1 .
AF061744 mRNA. Translation: AAD03267.1 .
CCDSi CCDS37031.1. [O35601-1 ]
RefSeqi NP_001265198.1. NM_001278269.1. [O35601-2 ]
NP_035945.1. NM_011815.5. [O35601-1 ]
UniGenei Mm.170905.

3D structure databases

ProteinModelPortali O35601.
SMRi O35601. Positions 494-567, 725-801.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204772. 3 interactions.
DIPi DIP-41350N.
IntActi O35601. 2 interactions.
MINTi MINT-244320.
STRINGi 10090.ENSMUSP00000087947.

PTM databases

PhosphoSitei O35601.

Proteomic databases

MaxQBi O35601.
PaxDbi O35601.
PRIDEi O35601.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000090461 ; ENSMUSP00000087947 ; ENSMUSG00000022148 . [O35601-1 ]
GeneIDi 23880.
KEGGi mmu:23880.
UCSCi uc007vdi.1. mouse. [O35601-1 ]
uc007vdk.1. mouse. [O35601-2 ]

Organism-specific databases

CTDi 2533.
MGIi MGI:1346327. Fyb.

Phylogenomic databases

eggNOGi NOG18948.
GeneTreei ENSGT00530000063460.
HOGENOMi HOG000008686.
HOVERGENi HBG005774.
InParanoidi O35601.
KOi K17698.
OMAi ACCDVKG.
OrthoDBi EOG70W3CP.
PhylomeDBi O35601.
TreeFami TF337003.

Enzyme and pathway databases

Reactomei REACT_215628. Signal regulatory protein (SIRP) family interactions.
REACT_225768. Generation of second messenger molecules.

Miscellaneous databases

NextBioi 303607.
PMAP-CutDB O35601.
PROi O35601.
SOURCEi Search...

Gene expression databases

Bgeei O35601.
CleanExi MM_FYB.
ExpressionAtlasi O35601. baseline and differential.
Genevestigatori O35601.

Family and domain databases

InterProi IPR029294. hSH3.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view ]
Pfami PF14603. hSH3. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view ]
SMARTi SM00326. SH3. 1 hit.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of a novel T-cell protein FYB that binds FYN and SH2-domain-containing leukocyte protein 76 and modulates interleukin 2 production."
    da Silva A.J., Li Z., de Vera C., Canto E., Findell P., Rudd C.E.
    Proc. Natl. Acad. Sci. U.S.A. 94:7493-7498(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM FYB-120), INTERACTION WITH FYN AND LCP2, FUNCTION.
    Tissue: T-cell lymphoma.
  2. "Novel isoform of lymphoid adaptor FYN-T-binding protein (FYB-130) interacts with SLP-76 and up-regulates interleukin 2 production."
    Veale M., Raab M., Li Z., da Silva A.J., Kraeft S.-K., Weremowicz S., Morton C.C., Rudd C.E.
    J. Biol. Chem. 274:28427-28435(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM FYB-130), INTERACTION WITH FYN AND LCP2, FUNCTION.
    Tissue: Hybridoma.
  3. "SHPS-1 is a scaffold for assembling distinct adhesion-regulated multi-protein complexes in macrophages."
    Timms J.F., Swanson K.D., Marie-Cardine A., Raab M., Rudd C.E., Schraven B., Neel B.G.
    Curr. Biol. 9:927-930(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SKAP2, IDENTIFICATION IN A COMPLEX WITH SKAP2 AND PTPNS1.
  4. "The Yersinia tyrosine phosphatase YopH targets a novel adhesion-regulated signalling complex in macrophages."
    Black D.S., Marie-Cardine A., Schraven B., Bliska J.B.
    Cell. Microbiol. 2:401-414(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SKAP2, IDENTIFICATION IN A COMPLEX WITH SKAP2 AND PTPNS1, IDENTIFICATION IN A COMPLEX WITH SKAP2 AND LILRB3.
  5. "ADAP is required for normal alphaIIb-beta3 activation by VWF/GP Ib-IX-V and other agonists."
    Kasirer-Friede A., Moran B., Nagrampa-Orje J., Swanson K., Ruggeri Z.M., Schraven B., Neel B.G., Koretzky G., Shattil S.J.
    Blood 109:1018-1025(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SKAP2, FUNCTION, DISRUPTION PHENOTYPE.
  6. "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
    Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
    J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-559 AND SER-561, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Mast cell.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  8. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-28, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiFYB_MOUSE
AccessioniPrimary (citable) accession number: O35601
Secondary accession number(s): Q9Z2H3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: December 1, 2000
Last modified: October 29, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3