Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O35601

- FYB_MOUSE

UniProt

O35601 - FYB_MOUSE

Protein

FYN-binding protein

Gene

Fyb

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (01 Dec 2000)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    May play a role in linking T-cell signaling to remodeling of the actin cytoskeleton By similarity. Acts as an adapter protein of the FYN and LCP2 signaling cascades in T-cells. Modulates the expression of interleukin-2 (IL-2). Involved in platelet activation. Prevents the degradation of SKAP1 and SKAP2.By similarity3 Publications

    Enzyme and pathway databases

    ReactomeiREACT_215628. Signal regulatory protein (SIRP) family interactions.
    REACT_225768. Generation of second messenger molecules.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    FYN-binding protein
    Alternative name(s):
    Adhesion and degranulation promoting adaptor protein
    Short name:
    ADAP
    FYB-120/130
    Short name:
    p120/p130
    FYN-T-binding protein
    SLAP-130
    SLP-76-associated phosphoprotein
    Gene namesi
    Name:Fyb
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:1346327. Fyb.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Slight defects in platelet function.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 819818FYN-binding proteinPRO_0000087397Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei3 – 31N6-acetyllysineBy similarity
    Modified residuei28 – 281Phosphoserine1 Publication
    Modified residuei46 – 461PhosphoserineBy similarity
    Modified residuei445 – 4451PhosphoserineBy similarity
    Modified residuei559 – 5591Phosphotyrosine1 Publication
    Modified residuei561 – 5611Phosphoserine1 Publication
    Modified residuei687 – 6871PhosphotyrosineBy similarity

    Post-translational modificationi

    T-cell receptor ligation leads to increased tyrosine phosphorylation.2 Publications

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiO35601.
    PaxDbiO35601.
    PRIDEiO35601.

    PTM databases

    PhosphoSiteiO35601.

    Miscellaneous databases

    PMAP-CutDBO35601.

    Expressioni

    Tissue specificityi

    Expressed in hematopoietic tissues such as myeloid and T-cells, spleen and thymus. Not expressed in B-cells, nor in non-lymphoid tissues. FYB-130 is preferentially expressed in mature T-cells compared to FYB-120, whereas thymocytes showed a greater relative amount of FYB-120.

    Gene expression databases

    ArrayExpressiO35601.
    BgeeiO35601.
    CleanExiMM_FYB.
    GenevestigatoriO35601.

    Interactioni

    Subunit structurei

    Interacts with FYN, LCP2, SKAP1 and SKAP2. Part of a complex consisting of SKAP2, FYB and PTPNS1. Part of a complex consisting of SKAP2, FYB and LILRB3. Interacts with FASLG. Interacts with EVL By similarity.By similarity

    Protein-protein interaction databases

    BioGridi204772. 3 interactions.
    DIPiDIP-41350N.
    IntActiO35601. 2 interactions.
    MINTiMINT-244320.
    STRINGi10090.ENSMUSP00000087947.

    Structurei

    3D structure databases

    ProteinModelPortaliO35601.
    SMRiO35601. Positions 494-567, 725-801.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini699 – 75658SH3Add
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili448 – 49548Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi479 – 49315Nuclear localization signalSequence AnalysisAdd
    BLAST
    Motifi584 – 5874SH2-binding; to LCP2
    Motifi615 – 6184SH2-binding; to FYN
    Motifi710 – 73627Nuclear localization signalSequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi343 – 3486Poly-Pro
    Compositional biasi381 – 3855Poly-Pro
    Compositional biasi606 – 6094Poly-Pro
    Compositional biasi622 – 6254Poly-Asp
    Compositional biasi696 – 6994Poly-Pro

    Sequence similaritiesi

    Contains 1 SH3 domain.Curated

    Keywords - Domaini

    Coiled coil, SH3 domain

    Phylogenomic databases

    eggNOGiNOG18948.
    GeneTreeiENSGT00530000063460.
    HOGENOMiHOG000008686.
    HOVERGENiHBG005774.
    InParanoidiO35601.
    KOiK17698.
    OMAiACCDVKG.
    OrthoDBiEOG70W3CP.
    PhylomeDBiO35601.
    TreeFamiTF337003.

    Family and domain databases

    InterProiIPR029294. hSH3.
    IPR011511. SH3_2.
    IPR001452. SH3_domain.
    [Graphical view]
    PfamiPF14603. hSH3. 1 hit.
    PF07653. SH3_2. 1 hit.
    [Graphical view]
    SMARTiSM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 2 hits.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform FYB-130 (identifier: O35601-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAKFNTGSNP TEEAATSSRP FKVAGQSSPS GIQSRKNLFD NQGNASPPAG    50
    PSSMPKFGTT KPPLAAKPTY EEKPEKEPKP PFLKPTGGSP RFGTQPNSVS 100
    RDPEVKVGFL KPVSPKPTSL TKEDSKPVVL RPPGNKLHNL NQESDLKTPG 150
    PKPGPAPPVP ENELKPGFSK VAGAKSKFMP AAQDTDSKPR FPRHTFGQKP 200
    SLSTEDSQEE NTSKNVPVQK GSPVQLGAKS KGAPFKPPKE DPEDKDHGAP 250
    SSPFPGVVLK PAASRGSPGL SKNFEEKKED RKTDLAKNIF LNKLNQEEPA 300
    RFPKAPSKLT AGTPWGQSQE KEGDKNSATP KQKALPPLSV LGPPPPKPNR 350
    PPNVDLTRFR KADSANSATK SQTPYSTTSL PPPPPTHPAS QPPLPASHPA 400
    HPPVPSLPPR NIKPPLDLKH PINDENQDGV MHSDGTGNLE EEQESEGETY 450
    EDIDSSKERD KKREKEEKKR LELERKEQKE REKKEQELKK KFKLTGPIQV 500
    IHHAKACCDV KGGKNELSFK QGEDIEIIRI TDNPEGKWLG RTARGSYGYI 550
    KTTAVEIDYD SLKRKKNSLN AVPPRLVEDD QDVYDDVAEQ DAPNSHGQSG 600
    SGGMFPPPPT DDEIYDGIEE EDDDDGSVPQ VDEKTNAWSW GILKMLKGKD 650
    DRKKSIREKP KVSESDNNEG SSLPSQHKQL DVGEEVYDDV DASDFPPPPA 700
    EMSQGMSVGR AKTEEKDPKK LKKQEKEEKD LRKKFKYDGE IRVLYSTKVA 750
    SSLTSKKWGA RDLQIKPGES LEVIQSTDDT KVLCRNEEGK YGYVLRSYLV 800
    DNDGEIYDDI ADGCIYDND 819
    Length:819
    Mass (Da):90,055
    Last modified:December 1, 2000 - v2
    Checksum:i6222CC7EF1CA2BBD
    GO
    Isoform FYB-120 (identifier: O35601-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         627-672: Missing.

    Show »
    Length:773
    Mass (Da):84,870
    Checksum:iA3EC5C9AA7124B69
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei627 – 67246Missing in isoform FYB-120. 1 PublicationVSP_004261Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF001863 mRNA. Translation: AAB62227.1.
    AF061744 mRNA. Translation: AAD03267.1.
    CCDSiCCDS37031.1. [O35601-1]
    RefSeqiNP_001265198.1. NM_001278269.1. [O35601-2]
    NP_035945.1. NM_011815.5. [O35601-1]
    UniGeneiMm.170905.

    Genome annotation databases

    EnsembliENSMUST00000090461; ENSMUSP00000087947; ENSMUSG00000022148. [O35601-1]
    GeneIDi23880.
    KEGGimmu:23880.
    UCSCiuc007vdi.1. mouse. [O35601-1]
    uc007vdk.1. mouse. [O35601-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF001863 mRNA. Translation: AAB62227.1 .
    AF061744 mRNA. Translation: AAD03267.1 .
    CCDSi CCDS37031.1. [O35601-1 ]
    RefSeqi NP_001265198.1. NM_001278269.1. [O35601-2 ]
    NP_035945.1. NM_011815.5. [O35601-1 ]
    UniGenei Mm.170905.

    3D structure databases

    ProteinModelPortali O35601.
    SMRi O35601. Positions 494-567, 725-801.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204772. 3 interactions.
    DIPi DIP-41350N.
    IntActi O35601. 2 interactions.
    MINTi MINT-244320.
    STRINGi 10090.ENSMUSP00000087947.

    PTM databases

    PhosphoSitei O35601.

    Proteomic databases

    MaxQBi O35601.
    PaxDbi O35601.
    PRIDEi O35601.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000090461 ; ENSMUSP00000087947 ; ENSMUSG00000022148 . [O35601-1 ]
    GeneIDi 23880.
    KEGGi mmu:23880.
    UCSCi uc007vdi.1. mouse. [O35601-1 ]
    uc007vdk.1. mouse. [O35601-2 ]

    Organism-specific databases

    CTDi 2533.
    MGIi MGI:1346327. Fyb.

    Phylogenomic databases

    eggNOGi NOG18948.
    GeneTreei ENSGT00530000063460.
    HOGENOMi HOG000008686.
    HOVERGENi HBG005774.
    InParanoidi O35601.
    KOi K17698.
    OMAi ACCDVKG.
    OrthoDBi EOG70W3CP.
    PhylomeDBi O35601.
    TreeFami TF337003.

    Enzyme and pathway databases

    Reactomei REACT_215628. Signal regulatory protein (SIRP) family interactions.
    REACT_225768. Generation of second messenger molecules.

    Miscellaneous databases

    NextBioi 303607.
    PMAP-CutDB O35601.
    PROi O35601.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O35601.
    Bgeei O35601.
    CleanExi MM_FYB.
    Genevestigatori O35601.

    Family and domain databases

    InterProi IPR029294. hSH3.
    IPR011511. SH3_2.
    IPR001452. SH3_domain.
    [Graphical view ]
    Pfami PF14603. hSH3. 1 hit.
    PF07653. SH3_2. 1 hit.
    [Graphical view ]
    SMARTi SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of a novel T-cell protein FYB that binds FYN and SH2-domain-containing leukocyte protein 76 and modulates interleukin 2 production."
      da Silva A.J., Li Z., de Vera C., Canto E., Findell P., Rudd C.E.
      Proc. Natl. Acad. Sci. U.S.A. 94:7493-7498(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM FYB-120), INTERACTION WITH FYN AND LCP2, FUNCTION.
      Tissue: T-cell lymphoma.
    2. "Novel isoform of lymphoid adaptor FYN-T-binding protein (FYB-130) interacts with SLP-76 and up-regulates interleukin 2 production."
      Veale M., Raab M., Li Z., da Silva A.J., Kraeft S.-K., Weremowicz S., Morton C.C., Rudd C.E.
      J. Biol. Chem. 274:28427-28435(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM FYB-130), INTERACTION WITH FYN AND LCP2, FUNCTION.
      Tissue: Hybridoma.
    3. "SHPS-1 is a scaffold for assembling distinct adhesion-regulated multi-protein complexes in macrophages."
      Timms J.F., Swanson K.D., Marie-Cardine A., Raab M., Rudd C.E., Schraven B., Neel B.G.
      Curr. Biol. 9:927-930(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SKAP2, IDENTIFICATION IN A COMPLEX WITH SKAP2 AND PTPNS1.
    4. "The Yersinia tyrosine phosphatase YopH targets a novel adhesion-regulated signalling complex in macrophages."
      Black D.S., Marie-Cardine A., Schraven B., Bliska J.B.
      Cell. Microbiol. 2:401-414(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SKAP2, IDENTIFICATION IN A COMPLEX WITH SKAP2 AND PTPNS1, IDENTIFICATION IN A COMPLEX WITH SKAP2 AND LILRB3.
    5. "ADAP is required for normal alphaIIb-beta3 activation by VWF/GP Ib-IX-V and other agonists."
      Kasirer-Friede A., Moran B., Nagrampa-Orje J., Swanson K., Ruggeri Z.M., Schraven B., Neel B.G., Koretzky G., Shattil S.J.
      Blood 109:1018-1025(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SKAP2, FUNCTION, DISRUPTION PHENOTYPE.
    6. "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
      Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
      J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-559 AND SER-561, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Mast cell.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    8. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-28, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiFYB_MOUSE
    AccessioniPrimary (citable) accession number: O35601
    Secondary accession number(s): Q9Z2H3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3