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Protein

FYN-binding protein 1

Gene

Fyb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in linking T-cell signaling to remodeling of the actin cytoskeleton (By similarity). Acts as an adapter protein of the FYN and LCP2 signaling cascades in T-cells. Modulates the expression of interleukin-2 (IL-2). Involved in platelet activation. Prevents the degradation of SKAP1 and SKAP2.By similarity3 Publications

GO - Molecular functioni

GO - Biological processi

  • immune response Source: InterPro
  • protein localization to plasma membrane Source: MGI
  • signal transduction Source: GO_Central
  • T cell receptor signaling pathway Source: Reactome

Enzyme and pathway databases

ReactomeiR-MMU-202433. Generation of second messenger molecules.
R-MMU-391160. Signal regulatory protein family interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
FYN-binding protein 1
Alternative name(s):
Adhesion and degranulation promoting adaptor protein
Short name:
ADAP
FYB-120/130
Short name:
p120/p130
FYN-T-binding protein
SLAP-130
SLP-76-associated phosphoprotein
Gene namesi
Name:Fyb1
Synonyms:Fyb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1346327. Fyb.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Slight defects in platelet function.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000873971 – 819FYN-binding protein 1Add BLAST819

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei3N6-acetyllysineBy similarity1
Modified residuei28PhosphoserineCombined sources1
Modified residuei46PhosphoserineCombined sources1
Modified residuei222PhosphoserineCombined sources1
Modified residuei318PhosphoserineBy similarity1
Modified residuei445PhosphoserineCombined sources1
Modified residuei559PhosphotyrosineCombined sources1
Modified residuei561PhosphoserineCombined sources1
Modified residuei568PhosphoserineCombined sources1
Modified residuei687PhosphotyrosineBy similarity1

Post-translational modificationi

T-cell receptor ligation leads to increased tyrosine phosphorylation.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO35601.
MaxQBiO35601.
PaxDbiO35601.
PeptideAtlasiO35601.
PRIDEiO35601.

PTM databases

iPTMnetiO35601.
PhosphoSitePlusiO35601.

Miscellaneous databases

PMAP-CutDBiO35601.

Expressioni

Tissue specificityi

Expressed in hematopoietic tissues such as myeloid and T-cells, spleen and thymus. Not expressed in B-cells, nor in non-lymphoid tissues. FYB-130 is preferentially expressed in mature T-cells compared to FYB-120, whereas thymocytes showed a greater relative amount of FYB-120. Expressed in podocytes.1 Publication

Gene expression databases

BgeeiENSMUSG00000022148.
CleanExiMM_FYB.
ExpressionAtlasiO35601. baseline and differential.
GenevisibleiO35601. MM.

Interactioni

Subunit structurei

Interacts with FYN, LCP2, SKAP1 and SKAP2. Part of a complex consisting of SKAP2, FYB1 and PTPNS1. Part of a complex consisting of SKAP2, FYB1 and LILRB3. Interacts with FASLG. Interacts with EVL (By similarity). Interacts with TMEM47 (PubMed:21881001). Interacts with LCK (By similarity).By similarity1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204772. 3 interactors.
CORUMiO35601.
DIPiDIP-41350N.
IntActiO35601. 6 interactors.
MINTiMINT-244320.
STRINGi10090.ENSMUSP00000087947.

Structurei

3D structure databases

ProteinModelPortaliO35601.
SMRiO35601.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini499 – 560SH3 1PROSITE-ProRule annotationAdd BLAST62
Domaini736 – 804SH3 2PROSITE-ProRule annotationAdd BLAST69

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili448 – 495Sequence analysisAdd BLAST48

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi479 – 493Nuclear localization signalSequence analysisAdd BLAST15
Motifi584 – 587SH2-binding; to LCP24
Motifi615 – 618SH2-binding; to FYN4
Motifi710 – 736Nuclear localization signalSequence analysisAdd BLAST27

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi343 – 348Poly-Pro6
Compositional biasi381 – 385Poly-Pro5
Compositional biasi606 – 609Poly-Pro4
Compositional biasi622 – 625Poly-Asp4
Compositional biasi696 – 699Poly-Pro4

Keywords - Domaini

Coiled coil, Repeat, SH3 domain

Phylogenomic databases

eggNOGiENOG410IEQF. Eukaryota.
ENOG410YD3U. LUCA.
GeneTreeiENSGT00530000063460.
HOGENOMiHOG000008686.
HOVERGENiHBG005774.
InParanoidiO35601.
KOiK17698.
OMAiSWGILKM.
OrthoDBiEOG091G04X1.
PhylomeDBiO35601.
TreeFamiTF337003.

Family and domain databases

CDDicd11867. hSH3_ADAP. 1 hit.
InterProiView protein in InterPro
IPR030635. FYB.
IPR035540. FYB_hSH3.
IPR029294. hSH3.
IPR036028. SH3-like_dom_sf.
IPR001452. SH3_domain.
PANTHERiPTHR16830:SF13. PTHR16830:SF13. 1 hit.
PfamiView protein in Pfam
PF14603. hSH3. 1 hit.
PF07653. SH3_2. 1 hit.
SMARTiView protein in SMART
SM00326. SH3. 1 hit.
SUPFAMiSSF50044. SSF50044. 2 hits.
PROSITEiView protein in PROSITE
PS50002. SH3. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform FYB-130 (identifier: O35601-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAKFNTGSNP TEEAATSSRP FKVAGQSSPS GIQSRKNLFD NQGNASPPAG
60 70 80 90 100
PSSMPKFGTT KPPLAAKPTY EEKPEKEPKP PFLKPTGGSP RFGTQPNSVS
110 120 130 140 150
RDPEVKVGFL KPVSPKPTSL TKEDSKPVVL RPPGNKLHNL NQESDLKTPG
160 170 180 190 200
PKPGPAPPVP ENELKPGFSK VAGAKSKFMP AAQDTDSKPR FPRHTFGQKP
210 220 230 240 250
SLSTEDSQEE NTSKNVPVQK GSPVQLGAKS KGAPFKPPKE DPEDKDHGAP
260 270 280 290 300
SSPFPGVVLK PAASRGSPGL SKNFEEKKED RKTDLAKNIF LNKLNQEEPA
310 320 330 340 350
RFPKAPSKLT AGTPWGQSQE KEGDKNSATP KQKALPPLSV LGPPPPKPNR
360 370 380 390 400
PPNVDLTRFR KADSANSATK SQTPYSTTSL PPPPPTHPAS QPPLPASHPA
410 420 430 440 450
HPPVPSLPPR NIKPPLDLKH PINDENQDGV MHSDGTGNLE EEQESEGETY
460 470 480 490 500
EDIDSSKERD KKREKEEKKR LELERKEQKE REKKEQELKK KFKLTGPIQV
510 520 530 540 550
IHHAKACCDV KGGKNELSFK QGEDIEIIRI TDNPEGKWLG RTARGSYGYI
560 570 580 590 600
KTTAVEIDYD SLKRKKNSLN AVPPRLVEDD QDVYDDVAEQ DAPNSHGQSG
610 620 630 640 650
SGGMFPPPPT DDEIYDGIEE EDDDDGSVPQ VDEKTNAWSW GILKMLKGKD
660 670 680 690 700
DRKKSIREKP KVSESDNNEG SSLPSQHKQL DVGEEVYDDV DASDFPPPPA
710 720 730 740 750
EMSQGMSVGR AKTEEKDPKK LKKQEKEEKD LRKKFKYDGE IRVLYSTKVA
760 770 780 790 800
SSLTSKKWGA RDLQIKPGES LEVIQSTDDT KVLCRNEEGK YGYVLRSYLV
810
DNDGEIYDDI ADGCIYDND
Length:819
Mass (Da):90,055
Last modified:December 1, 2000 - v2
Checksum:i6222CC7EF1CA2BBD
GO
Isoform FYB-120 (identifier: O35601-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     627-672: Missing.

Show »
Length:773
Mass (Da):84,870
Checksum:iA3EC5C9AA7124B69
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004261627 – 672Missing in isoform FYB-120. 1 PublicationAdd BLAST46

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF001863 mRNA. Translation: AAB62227.1.
AF061744 mRNA. Translation: AAD03267.1.
CCDSiCCDS37031.1. [O35601-1]
RefSeqiNP_001265198.1. NM_001278269.1. [O35601-2]
NP_035945.1. NM_011815.5. [O35601-1]
UniGeneiMm.170905.

Genome annotation databases

EnsembliENSMUST00000090461; ENSMUSP00000087947; ENSMUSG00000022148. [O35601-1]
GeneIDi23880.
KEGGimmu:23880.
UCSCiuc007vdi.2. mouse. [O35601-1]
uc007vdk.2. mouse. [O35601-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiFYB1_MOUSE
AccessioniPrimary (citable) accession number: O35601
Secondary accession number(s): Q9Z2H3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: December 1, 2000
Last modified: November 22, 2017
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot