Reviewed, UniProtKB/Swiss-Prot O35599 (OPSG_MOUSE)

Names and origin

Protein names

Recommended name:
    Green-sensitive opsin
Alternative name(s):
    Green cone photoreceptor pigment
    Medium wavelength-sensitive cone opsin
      Short name=M opsin

Gene names

Name:	Opn1mw
Synonyms:	Gcp

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal. May increase spectral sensitivity in dim light. Ref.2
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Expresssed in cone photoreceptor cells. Ref.2
Post-translational modification	Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

Ontologies

Keywords
Biological process	Sensory transduction Vision
Cellular component	Membrane
Domain	Transmembrane
Ligand	Chromophore
Molecular function	G-protein coupled receptor Photoreceptor protein Receptor Retinal protein Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW phototransduction Inferred from electronic annotation. Source: UniProtKB-KW protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW photoreceptor outer segment Inferred from direct assay. Source: MGI
Molecular function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW photoreceptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Green-sensitive opsin		PRO_0000197786
Regions
	Topological domain	1 – 47	47	Extracellular Potential
	Transmembrane	48 – 72	25	1 Potential
	Topological domain	73 – 84	12	Cytoplasmic Potential
	Transmembrane	85 – 110	26	2 Potential
	Topological domain	111 – 124	14	Extracellular Potential
	Transmembrane	125 – 144	20	3 Potential
	Topological domain	145 – 163	19	Cytoplasmic Potential
	Transmembrane	164 – 187	24	4 Potential
	Topological domain	188 – 213	26	Extracellular Potential
	Transmembrane	214 – 241	28	5 Potential
	Topological domain	242 – 263	22	Cytoplasmic Potential
	Transmembrane	264 – 287	24	6 Potential
	Topological domain	288 – 295	8	Extracellular Potential
	Transmembrane	296 – 320	25	7 Potential
	Topological domain	321 – 359	39	Cytoplasmic Potential
Amino acid modifications
	Modified residue	307	1	N6-(retinylidene)lysine
	Glycosylation	29	1	N-linked (GlcNAc...) Probable
	Disulfide bond	121 ↔ 198		Potential

Sequences

	Sequence		Length	Mass (Da)
	O35599-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 1BB5F531A6FAE357	FASTA	359	40,218
	10 20 30 40 50 60 MAQRLTGEQT LDHYEDSTHA SIFTYTNSNS TKGPFEGPNY HIAPRWVYHL TSTWMILVVV 70 80 90 100 110 120 ASVFTNGLVL AATMRFKKLR HPLNWILVNL AVADLAETII ASTISVVNQI YGYFVLGHPL 130 140 150 160 170 180 CVIEGYIVSL CGITGLWSLA IISWERWLVV CKPFGNVRFD AKLATVGIVF SWVWAAIWTA 190 200 210 220 230 240 PPIFGWSRYW PYGLKTSCGP DVFSGTSYPG VQSYMMVLMV TCCIFPLSII VLCYLQVWLA 250 260 270 280 290 300 IRAVAKQQKE SESTQKAEKE VTRMVVVMVF AYCLCWGPYT FFACFATAHP GYAFHPLVAS 310 320 330 340 350 LPSYFAKSAT IYNPIIYVFM NRQFRNCILH LFGKKVDDSS ELSSTSKTEV SSVSSVSPA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mechanisms of spectral tuning in the mouse green cone pigment." Sun H., Macke J.P., Nathans J. Proc. Natl. Acad. Sci. U.S.A. 94:8860-8865(1997) [PubMed: 9238068] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The murine cone photoreceptor: a single cone type expresses both S and M opsins with retinal spatial patterning." Applebury M.L., Antoch M.P., Baxter L.C., Chun L.Y., Falk J.D., Farhangfar F., Kage K., Krzystolik M.G., Lyass L.A., Robbins J.T. Neuron 27:513-523(2000) [PubMed: 11055434] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY. Strain: BALB/c and C57BL/10.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Retina.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF011389 mRNA. Translation: AAB64302.1. AF190672 mRNA. Translation: AAG17991.1. AF191085 , AF191080, AF191081, AF191082, AF191083, AF191084 Genomic DNA. Translation: AAG17992.1. BC014826 mRNA. Translation: AAH14826.1.
IPI	IPI00131882.
RefSeq	NP_032132.1.
UniGene	Mm.284825
3D structure databases
HSSP	HSSP built from PDB template 1FDF based on UniProtKB P02699.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Genome annotation databases
Ensembl	ENSMUSG00000031394. Mus musculus. [Contig view]
GeneID	14539.
KEGG	mmu:14539.
Organism-specific databases
MGI	MGI:1097692. Opn1mw.
Phylogenomic databases
HOVERGEN	O35599.
Gene expression databases
ArrayExpress	O35599.
Bgee	O35599.
GermOnline	ENSMUSG00000031394. Mus musculus.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR001760. Opsin. IPR000378. Opsin_red/grn. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00238. OPSIN. PR00575. OPSINREDGRN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. PS00238. OPSIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	286212.
SOURCE	Search...

Entry information

Entry name

OPSG_MOUSE

Accession

Primary (citable) accession number: O35599
Secondary accession number(s): Q548Z4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)