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Protein

Medium-wave-sensitive opsin 1

Gene

Opn1mw

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal. May increase spectral sensitivity in dim light.1 Publication

GO - Molecular functioni

  1. G-protein coupled receptor activity Source: UniProtKB-KW
  2. photoreceptor activity Source: UniProtKB-KW

GO - Biological processi

  1. phototransduction Source: UniProtKB-KW
  2. positive regulation of cytokinesis Source: MGI
  3. protein-chromophore linkage Source: UniProtKB-KW
  4. visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Photoreceptor protein, Receptor, Retinal protein, Transducer

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

Chromophore

Enzyme and pathway databases

ReactomeiREACT_280434. The retinoid cycle in cones (daylight vision).
REACT_306607. Retinoid cycle disease events.
REACT_331048. G alpha (i) signalling events.
REACT_332325. Opsins.

Names & Taxonomyi

Protein namesi
Recommended name:
Medium-wave-sensitive opsin 1
Alternative name(s):
Green cone photoreceptor pigment
Green-sensitive opsin
Short name:
M opsin
Medium wavelength-sensitive cone opsin
Gene namesi
Name:Opn1mw
Synonyms:Gcp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1097692. Opn1mw.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4747ExtracellularSequence AnalysisAdd
BLAST
Transmembranei48 – 7225Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini73 – 8412CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei85 – 11026Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini111 – 12414ExtracellularSequence AnalysisAdd
BLAST
Transmembranei125 – 14420Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini145 – 16319CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei164 – 18724Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini188 – 21326ExtracellularSequence AnalysisAdd
BLAST
Transmembranei214 – 24128Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini242 – 26322CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei264 – 28724Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini288 – 2958ExtracellularSequence Analysis
Transmembranei296 – 32025Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini321 – 35939CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. photoreceptor outer segment Source: MGI
  3. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 359359Medium-wave-sensitive opsin 1PRO_0000197786Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi29 – 291N-linked (GlcNAc...)Curated
Disulfide bondi121 ↔ 198PROSITE-ProRule annotation
Modified residuei307 – 3071N6-(retinylidene)lysine

Post-translational modificationi

Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiO35599.

PTM databases

PhosphoSiteiO35599.

Expressioni

Tissue specificityi

Expressed in cone photoreceptor cells.1 Publication

Gene expression databases

BgeeiO35599.
ExpressionAtlasiO35599. baseline and differential.
GenevestigatoriO35599.

Structurei

3D structure databases

ProteinModelPortaliO35599.
SMRiO35599. Positions 16-354.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG240324.
GeneTreeiENSGT00760000118977.
HOGENOMiHOG000253932.
HOVERGENiHBG107442.
InParanoidiO35599.
KOiK04251.
OrthoDBiEOG72NRQJ.
PhylomeDBiO35599.
TreeFamiTF324998.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001760. Opsin.
IPR000378. Opsin_red/grn.
IPR027430. Retinal_BS.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00238. OPSIN.
PR00575. OPSINREDGRN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
PS00238. OPSIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35599-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQRLTGEQT LDHYEDSTHA SIFTYTNSNS TKGPFEGPNY HIAPRWVYHL
60 70 80 90 100
TSTWMILVVV ASVFTNGLVL AATMRFKKLR HPLNWILVNL AVADLAETII
110 120 130 140 150
ASTISVVNQI YGYFVLGHPL CVIEGYIVSL CGITGLWSLA IISWERWLVV
160 170 180 190 200
CKPFGNVRFD AKLATVGIVF SWVWAAIWTA PPIFGWSRYW PYGLKTSCGP
210 220 230 240 250
DVFSGTSYPG VQSYMMVLMV TCCIFPLSII VLCYLQVWLA IRAVAKQQKE
260 270 280 290 300
SESTQKAEKE VTRMVVVMVF AYCLCWGPYT FFACFATAHP GYAFHPLVAS
310 320 330 340 350
LPSYFAKSAT IYNPIIYVFM NRQFRNCILH LFGKKVDDSS ELSSTSKTEV

SSVSSVSPA
Length:359
Mass (Da):40,218
Last modified:January 1, 1998 - v1
Checksum:i1BB5F531A6FAE357
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF011389 mRNA. Translation: AAB64302.1.
AF190672 mRNA. Translation: AAG17991.1.
AF191085
, AF191080, AF191081, AF191082, AF191083, AF191084 Genomic DNA. Translation: AAG17992.1.
BC014826 mRNA. Translation: AAH14826.1.
CCDSiCCDS30220.1.
RefSeqiNP_032132.1. NM_008106.2.
UniGeneiMm.284825.

Genome annotation databases

EnsembliENSMUST00000033771; ENSMUSP00000033771; ENSMUSG00000031394.
GeneIDi14539.
KEGGimmu:14539.
UCSCiuc009tnv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF011389 mRNA. Translation: AAB64302.1.
AF190672 mRNA. Translation: AAG17991.1.
AF191085
, AF191080, AF191081, AF191082, AF191083, AF191084 Genomic DNA. Translation: AAG17992.1.
BC014826 mRNA. Translation: AAH14826.1.
CCDSiCCDS30220.1.
RefSeqiNP_032132.1. NM_008106.2.
UniGeneiMm.284825.

3D structure databases

ProteinModelPortaliO35599.
SMRiO35599. Positions 16-354.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiO35599.

Proteomic databases

PRIDEiO35599.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033771; ENSMUSP00000033771; ENSMUSG00000031394.
GeneIDi14539.
KEGGimmu:14539.
UCSCiuc009tnv.1. mouse.

Organism-specific databases

CTDi2652.
MGIiMGI:1097692. Opn1mw.

Phylogenomic databases

eggNOGiNOG240324.
GeneTreeiENSGT00760000118977.
HOGENOMiHOG000253932.
HOVERGENiHBG107442.
InParanoidiO35599.
KOiK04251.
OrthoDBiEOG72NRQJ.
PhylomeDBiO35599.
TreeFamiTF324998.

Enzyme and pathway databases

ReactomeiREACT_280434. The retinoid cycle in cones (daylight vision).
REACT_306607. Retinoid cycle disease events.
REACT_331048. G alpha (i) signalling events.
REACT_332325. Opsins.

Miscellaneous databases

NextBioi286212.
PROiO35599.
SOURCEiSearch...

Gene expression databases

BgeeiO35599.
ExpressionAtlasiO35599. baseline and differential.
GenevestigatoriO35599.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001760. Opsin.
IPR000378. Opsin_red/grn.
IPR027430. Retinal_BS.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00238. OPSIN.
PR00575. OPSINREDGRN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
PS00238. OPSIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mechanisms of spectral tuning in the mouse green cone pigment."
    Sun H., Macke J.P., Nathans J.
    Proc. Natl. Acad. Sci. U.S.A. 94:8860-8865(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The murine cone photoreceptor: a single cone type expresses both S and M opsins with retinal spatial patterning."
    Applebury M.L., Antoch M.P., Baxter L.C., Chun L.Y., Falk J.D., Farhangfar F., Kage K., Krzystolik M.G., Lyass L.A., Robbins J.T.
    Neuron 27:513-523(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY.
    Strain: BALB/c and C57BL/10.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Retina.

Entry informationi

Entry nameiOPSG_MOUSE
AccessioniPrimary (citable) accession number: O35599
Secondary accession number(s): Q548Z4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: April 1, 2015
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.