ID   PTC2_MOUSE              Reviewed;        1182 AA.
AC   O35595; A2AE82; Q546T0;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 2.
DT   24-JAN-2024, entry version 149.
DE   RecName: Full=Protein patched homolog 2;
DE            Short=PTC2;
GN   Name=Ptch2;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   TISSUE=Embryo;
RX   PubMed=9462734; DOI=10.1038/ng0298-104;
RA   Motoyama J., Takabatake T., Takeshima K., Hui C.-C.;
RT   "Ptch2, a second mouse Patched gene is co-expressed with Sonic hedgehog.";
RL   Nat. Genet. 18:104-106(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=129/SvJ; TISSUE=Liver;
RX   PubMed=12438747; DOI=10.1159/000064064;
RA   Froehlich L., Zhanquin L., Beier D.R., Lanske B.;
RT   "Genomic structure and refined chromosomal localization of the mouse Ptch2
RT   gene.";
RL   Cytogenet. Genome Res. 97:106-110(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 196-446.
RC   STRAIN=BALB/cJ; TISSUE=Neuroretina;
RX   PubMed=9237688; DOI=10.1016/s0014-5793(97)00645-5;
RA   Takabatake T., Ogawa M., Takahashi T.C., Mizuno M., Okamoto M.,
RA   Takeshima K.;
RT   "Hedgehog and patched gene expression in adult ocular tissues.";
RL   FEBS Lett. 410:485-489(1997).
CC   -!- FUNCTION: Plays a role in the control of cellular growth (By
CC       similarity). May have a role in epidermal development. May act as a
CC       receptor for Sonic hedgehog (SHH). {ECO:0000250|UniProtKB:Q9Y6C5}.
CC   -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC   -!- TISSUE SPECIFICITY: Expressed in epithelial cells of the developing
CC       hair, tooth and whisker.
CC   -!- DEVELOPMENTAL STAGE: Detected in 8.5 to 17.5 dpc embryos.
CC   -!- SIMILARITY: Belongs to the patched family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AJ133482; CAC88120.1; -; Genomic_DNA.
DR   EMBL; AJ133483; CAC88120.1; JOINED; Genomic_DNA.
DR   EMBL; AJ133484; CAC88120.1; JOINED; Genomic_DNA.
DR   EMBL; AJ133485; CAC88120.1; JOINED; Genomic_DNA.
DR   EMBL; AL671866; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AB010833; BAA24691.1; -; mRNA.
DR   CCDS; CCDS18526.1; -.
DR   PIR; T13952; T13952.
DR   RefSeq; NP_032984.1; NM_008958.3.
DR   AlphaFoldDB; O35595; -.
DR   SMR; O35595; -.
DR   STRING; 10090.ENSMUSP00000030443; -.
DR   GlyCosmos; O35595; 2 sites, No reported glycans.
DR   GlyGen; O35595; 2 sites.
DR   iPTMnet; O35595; -.
DR   PhosphoSitePlus; O35595; -.
DR   PaxDb; 10090-ENSMUSP00000030443; -.
DR   ProteomicsDB; 301894; -.
DR   Antibodypedia; 18516; 181 antibodies from 27 providers.
DR   DNASU; 19207; -.
DR   Ensembl; ENSMUST00000030443.12; ENSMUSP00000030443.6; ENSMUSG00000028681.12.
DR   GeneID; 19207; -.
DR   KEGG; mmu:19207; -.
DR   UCSC; uc008uhx.1; mouse.
DR   AGR; MGI:1095405; -.
DR   CTD; 8643; -.
DR   MGI; MGI:1095405; Ptch2.
DR   VEuPathDB; HostDB:ENSMUSG00000028681; -.
DR   eggNOG; KOG1935; Eukaryota.
DR   GeneTree; ENSGT00940000159901; -.
DR   HOGENOM; CLU_002506_0_0_1; -.
DR   InParanoid; O35595; -.
DR   OMA; DFISAQF; -.
DR   OrthoDB; 1219406at2759; -.
DR   PhylomeDB; O35595; -.
DR   TreeFam; TF106489; -.
DR   BioGRID-ORCS; 19207; 2 hits in 77 CRISPR screens.
DR   PRO; PR:O35595; -.
DR   Proteomes; UP000000589; Chromosome 4.
DR   RNAct; O35595; Protein.
DR   Bgee; ENSMUSG00000028681; Expressed in ciliary body and 190 other cell types or tissues.
DR   ExpressionAtlas; O35595; baseline and differential.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0097108; F:hedgehog family protein binding; ISO:MGI.
DR   GO; GO:0008158; F:hedgehog receptor activity; IBA:GO_Central.
DR   GO; GO:0005119; F:smoothened binding; ISO:MGI.
DR   GO; GO:0001709; P:cell fate determination; IGI:MGI.
DR   GO; GO:0009957; P:epidermal cell fate specification; IGI:MGI.
DR   GO; GO:0008544; P:epidermis development; IMP:MGI.
DR   GO; GO:0042633; P:hair cycle; IMP:MGI.
DR   GO; GO:0045879; P:negative regulation of smoothened signaling pathway; IBA:GO_Central.
DR   GO; GO:0045606; P:positive regulation of epidermal cell differentiation; IGI:MGI.
DR   GO; GO:0001558; P:regulation of cell growth; ISO:MGI.
DR   GO; GO:0043588; P:skin development; IMP:MGI.
DR   Gene3D; 1.20.1640.10; Multidrug efflux transporter AcrB transmembrane domain; 2.
DR   InterPro; IPR003392; Ptc/Disp.
DR   InterPro; IPR000731; SSD.
DR   InterPro; IPR004766; TM_rcpt_patched.
DR   NCBIfam; TIGR00918; 2A060602; 1.
DR   PANTHER; PTHR46022; PROTEIN PATCHED; 1.
DR   PANTHER; PTHR46022:SF3; PROTEIN PATCHED HOMOLOG 2; 1.
DR   Pfam; PF02460; Patched; 1.
DR   Pfam; PF12349; Sterol-sensing; 1.
DR   SUPFAM; SSF82866; Multidrug efflux transporter AcrB transmembrane domain; 2.
DR   PROSITE; PS50156; SSD; 1.
DR   Genevisible; O35595; MM.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Membrane; Receptor; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..1182
FT                   /note="Protein patched homolog 2"
FT                   /id="PRO_0000205971"
FT   TOPO_DOM        1..57
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        58..78
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        79..394
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        395..414
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        415..428
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        429..449
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        450..457
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        458..478
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        479..501
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        502..522
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        523..531
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        532..552
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        553..686
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        687..707
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        708..963
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        964..984
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        985..991
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        992..1012
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        1013
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        1014..1034
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        1035..1064
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        1065..1085
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        1086
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        1087..1107
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        1108..1182
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          394..552
FT                   /note="SSD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00199"
FT   CARBOHYD        370
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        812
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   1182 AA;  128586 MW;  715233D912C352F2 CRC64;
     MVRPLSLGEL PPSYTPPARS SAPHILAGSL QAPLWLRAYF QGLLFSLGCR IQKHCGKVLF
     LGLVAFGALA LGLRVAVIET DLEQLWVEVG SRVSQELHYT KEKLGEEAAY TSQMLIQTAH
     QEGGNVLTPE ALDLHLQAAL TASKVQVSLY GKSWDLNKIC YKSGVPLIEN GMIERMIEKL
     FPCVILTPLD CFWEGAKLQG GSAYLPGRPD IQWTNLDPQQ LLEELGPFAS LEGFRELLDK
     AQVGQAYVGR PCLDPDDPHC PPSAPNRHSR QAPNVAQELS GGCHGFSHKF MHWQEELLLG
     GTARDLQGQL LRAEALQSTF LLMSPRQLYE HFRGDYQTHD IGWSEEQASM VLQAWQRRFV
     QLAQEALPAN ASQQIHAFSS TTLDDILRAF SEVSTTRVVG GYLLMLAYAC VTMLRWDCAQ
     SQGAVGLAGV LLVALAVASG LGLCALLGIT FNAATTQVLP FLALGIGVDD IFLLAHAFTK
     APPDTPLPER MGECLRSTGT SVALTSVNNM VAFFMAALVP IPALRAFSLQ AAIVVGCNFA
     AVMLVFPAIL SLDLRRRHRQ RLDVLCCFSS PCSAQVIQML PQELGDRAVP VGIAHLTATV
     QAFTHCEASS QHVVTILPPQ AHLLSPASDP LGSELYSPGG STRDLLSQEE GTGPQAACRP
     LLCAHWTLAH FARYQFAPLL LQTRAKALVL LFFGALLGLS LYGATLVQDG LALTDVVPRG
     TKEHAFLSAQ LRYFSLYEVA LVTQGGFDYA HSQRALFDLH QRFSSLKAVL PPPATQAPRT
     WLHYYRSWLQ GIQAAFDQDW ASGRITCHSY RNGSEDGALA YKLLIQTGNA QEPLDFSQLT
     TRKLVDKEGL IPPELFYMGL TVWVSSDPLG LAASQANFYP PPPEWLHDKY DTTGENLRIP
     AAQPLEFAQF PFLLHGLQKT ADFVEAIEGA RAACTEAGQA GVHAYPSGSP FLFWEQYLGL
     RRCFLLAVCI LLVCTFLVCA LLLLSPWTAG LIVLVLAMMT VELFGIMGFL GIKLSAIPVV
     ILVASIGIGV EFTVHVALGF LTSHGSRNLR AASALEQTFA PVTDGAVSTL LGLLMLAGSN
     FDFIIRYFFV VLTVLTLLGL LHGLLLLPVL LSILGPPPQV VQVYKESPQT LNSAAPQRGG
     LRWDRPPTLP QSFARVTTSM TVALHPPPLP GAYVHPASEE PT
//