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O35587

- TMEDA_MESAU

UniProt

O35587 - TMEDA_MESAU

Protein

Transmembrane protein Tmp21

Gene

TMED10

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Involved in vesicular protein trafficking. Mainly functions in the early secretory pathway. Thought to act as cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and to be involved in vesicle coat formation at the cytoplasmic side. In COPII vesicle-mediated anterograde transport involved in the transport of GPI-anchored proteins and proposed to act together with TMED2 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER. Recognizes GPI anchors structural remodeled in the ER by PGAP1 and MPPE1. In COPI vesicle-mediated retrograde transport involved in the biogenesis of COPI vesicles and vesicle coat recruitment. On Golgi membranes, acts as primary receptor for ARF1-GDP which is involved in COPI-vesicle formation. Increases coatomer-dependent GTPase-activating activity of ARFGAP2. Involved in trafficking of G protein-coupled receptors (GPCRs). Regulates F2LR1, OPRM1 and P2RY4 exocytic trafficking from the Golgi to the plasma membrane thus contributing to receptor resensitization. Involved in trafficking of amyloid beta A4 protein and soluble APP-beta release (independent of modulation of gamma-secretase activity). As part of the presenilin-dependent gamma-secretase complex regulates gamma-cleavages of the amyloid beta A4 protein to yield amyloid-beta 40 (Abeta40). Involved in organization of the Golgi apparatus By similarity.By similarity

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. beta-amyloid formation Source: UniProtKB
    2. COPI-coated vesicle budding Source: UniProtKB
    3. protein transport Source: UniProtKB-KW
    4. retrograde vesicle-mediated transport, Golgi to ER Source: UniProtKB

    Keywords - Biological processi

    ER-Golgi transport, Protein transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transmembrane protein Tmp21
    Alternative name(s):
    21 kDa transmembrane-trafficking protein
    Integral membrane protein p23
    p24 family protein delta-1
    Short name:
    p24delta1
    Gene namesi
    Name:TMED10
    Synonyms:TMP21
    OrganismiMesocricetus auratus (Golden hamster)
    Taxonomic identifieri10036 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

    Subcellular locationi

    Golgi apparatuscis-Golgi network membrane By similarity; Single-pass type I membrane protein By similarity. Melanosome By similarity. Endoplasmic reticulum membrane By similarity; Single-pass type I membrane protein By similarity. Endoplasmic reticulum-Golgi intermediate compartment membrane By similarity; Single-pass type I membrane protein By similarity. Cytoplasmic vesiclesecretory vesicle membrane By similarity; Single-pass type I membrane protein By similarity. Cell membrane By similarity. Golgi apparatustrans-Golgi network membrane 1 Publication; Single-pass type I membrane protein 1 Publication
    Note: Cycles between compartments of the early secretatory pathway By similarity. Is one of the most abundant proteins in the cis-Golgi network. Detected in trans-Golgi network-derived transport vesicles.By similarity

    GO - Cellular componenti

    1. COPI-coated vesicle Source: UniProtKB
    2. endoplasmic reticulum-Golgi intermediate compartment membrane Source: UniProtKB-SubCell
    3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    4. gamma-secretase complex Source: UniProtKB
    5. Golgi membrane Source: GOC
    6. integral component of membrane Source: UniProtKB-KW
    7. melanosome Source: UniProtKB-SubCell
    8. plasma membrane Source: UniProtKB
    9. trans-Golgi network transport vesicle Source: UniProtKB
    10. transport vesicle membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131By similarityAdd
    BLAST
    Chaini32 – 219188Transmembrane protein Tmp21PRO_0000010400Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei171 – 1711Omega-N-methylated arginineBy similarity
    Modified residuei176 – 1761Omega-N-methylated arginineBy similarity
    Glycosylationi179 – 1791N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein, Methylation

    Interactioni

    Subunit structurei

    Predominantly homodimeric and to lesser extent monomeric in endoplasmic reticulum. Homodimer and monomer in endoplasmic reticulum-Golgi intermediate compartment and cis-Golgi network. Probably oligomerizes with other members of the EMP24/GP25L family such as TMED2, TMED7 and TMED9. Interacts with TMED2. Associates with the COPI vesicle coat (coatomer); TMED10:TMED2 heterotetramers are proposed to be involved in coatomer association. Interacts (via C-terminus) with COPG1; the interaction involves dimeric TMED10. Interacts with ARF1 (GDP-bound); the interaction probably involves a TMED10 oligomer. Interacts with SEC23A; indicative for an association of TMED10 with the COPII vesicle coat. Interacts with CD59, SEC24B, SEC24C and SEC24D. Interacts with MPPE1/PGAP5. Interacts with F2LR1. Interacts with KDELR2; the interaction is disrupted by KDELR2 ligand. Found in a complex composed at least of SURF4, TMED2 and TMED10. Associates with the presenilin-dependent gamma-secretase complex By similarity. Interacts with STX17; the interaction is direct By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CD59P139875EBI-4405327,EBI-297972From a different organism.
    TMED2Q153632EBI-4405327,EBI-998485From a different organism.

    Protein-protein interaction databases

    IntActiO35587. 6 interactions.
    MINTiMINT-4996182.

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini32 – 185154LumenalSequence AnalysisAdd
    BLAST
    Topological domaini207 – 21913CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei186 – 20621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini41 – 193153GOLDPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 142142Required for interaction with STX17By similarityAdd
    BLAST
    Regioni147 – 17832Required for TMED10 and TMED2 cis-Golgi network localizationBy similarityAdd
    BLAST
    Regioni204 – 21916Interaction with COPG1By similarityAdd
    BLAST
    Regioni207 – 21913Interaction with ARF1By similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi211 – 2199COPI vesicle coat-bindingSequence Analysis
    Motifi211 – 2122COPII vesicle coat-bindingSequence Analysis

    Domaini

    The lumenal domain mediates localization to the plasma membrane by partially overriding the ER retention by the cytoplasmic domain.By similarity

    Sequence similaritiesi

    Belongs to the EMP24/GP25L family.Curated
    Contains 1 GOLD domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOVERGENiHBG107275.

    Family and domain databases

    InterProiIPR009038. GOLD.
    IPR015720. TMP21-related.
    [Graphical view]
    PANTHERiPTHR22811. PTHR22811. 1 hit.
    PfamiPF01105. EMP24_GP25L. 1 hit.
    [Graphical view]
    PROSITEiPS50866. GOLD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O35587-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGSSGPLSW PGPRPCALLF LLLLGPSSVL AISFHLPVNS RKCLREEIHK    50
    DLLVTGAYEI TDQSGGAGGL RTHLKITDSA GHILYAKEDA TKGKFAFTTE 100
    DYDMFEVCFE SKGTGRIPDQ LVILDMKHGV EAKNYEEIAK VEKLKPLEVE 150
    LRRLEDLSES IVNDFAYMKK REEEMRDTNE STNTRVLYFS IFSMLCLIGL 200
    ATWQVFYLRR FFKAKKLIE 219
    Length:219
    Mass (Da):24,821
    Last modified:January 1, 1998 - v1
    Checksum:i1FCF2E49ABB565EB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ001513 mRNA. Translation: CAA04796.1.
    RefSeqiNP_001268617.1. NM_001281688.1.

    Genome annotation databases

    GeneIDi101832157.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ001513 mRNA. Translation: CAA04796.1 .
    RefSeqi NP_001268617.1. NM_001281688.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O35587. 6 interactions.
    MINTi MINT-4996182.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 101832157.

    Phylogenomic databases

    HOVERGENi HBG107275.

    Family and domain databases

    InterProi IPR009038. GOLD.
    IPR015720. TMP21-related.
    [Graphical view ]
    PANTHERi PTHR22811. PTHR22811. 1 hit.
    Pfami PF01105. EMP24_GP25L. 1 hit.
    [Graphical view ]
    PROSITEi PS50866. GOLD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Involvement of the transmembrane protein p23 in biosynthetic protein transport."
      Rojo M., Pepperkok R., Emery G., Kellner R., Stang E., Parton R.G., Gruenberg J.
      J. Cell Biol. 139:1119-1135(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Identification of components of trans-Golgi network-derived transport vesicles and detergent-insoluble complexes by nanoelectrospray tandem mass spectrometry."
      Shevchenko A., Keller P., Scheiffele P., Mann M., Simons K.
      Electrophoresis 18:2591-2600(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    3. "Selective export of human GPI-anchored proteins from the endoplasmic reticulum."
      Bonnon C., Wendeler M.W., Paccaud J.P., Hauri H.P.
      J. Cell Sci. 123:1705-1715(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CD59; SEC24B; SEC24C AND SEC24D.

    Entry informationi

    Entry nameiTMEDA_MESAU
    AccessioniPrimary (citable) accession number: O35587
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3