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O35587

- TMEDA_MESAU

UniProt

O35587 - TMEDA_MESAU

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Protein

Transmembrane protein Tmp21

Gene
TMED10, TMP21
Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in vesicular protein trafficking. Mainly functions in the early secretory pathway. Thought to act as cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and to be involved in vesicle coat formation at the cytoplasmic side. In COPII vesicle-mediated anterograde transport involved in the transport of GPI-anchored proteins and proposed to act together with TMED2 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER. Recognizes GPI anchors structural remodeled in the ER by PGAP1 and MPPE1. In COPI vesicle-mediated retrograde transport involved in the biogenesis of COPI vesicles and vesicle coat recruitment. On Golgi membranes, acts as primary receptor for ARF1-GDP which is involved in COPI-vesicle formation. Increases coatomer-dependent GTPase-activating activity of ARFGAP2. Involved in trafficking of G protein-coupled receptors (GPCRs). Regulates F2LR1, OPRM1 and P2RY4 exocytic trafficking from the Golgi to the plasma membrane thus contributing to receptor resensitization. Involved in trafficking of amyloid beta A4 protein and soluble APP-beta release (independent of modulation of gamma-secretase activity). As part of the presenilin-dependent gamma-secretase complex regulates gamma-cleavages of the amyloid beta A4 protein to yield amyloid-beta 40 (Abeta40). Involved in organization of the Golgi apparatus By similarity.

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. beta-amyloid formation Source: UniProtKB
  2. COPI-coated vesicle budding Source: UniProtKB
  3. protein transport Source: UniProtKB-KW
  4. retrograde vesicle-mediated transport, Golgi to ER Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane protein Tmp21
Alternative name(s):
21 kDa transmembrane-trafficking protein
Integral membrane protein p23
p24 family protein delta-1
Short name:
p24delta1
Gene namesi
Name:TMED10
Synonyms:TMP21
OrganismiMesocricetus auratus (Golden hamster)
Taxonomic identifieri10036 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

Subcellular locationi

Golgi apparatuscis-Golgi network membrane; Single-pass type I membrane protein By similarity. Melanosome By similarity. Endoplasmic reticulum membrane; Single-pass type I membrane protein By similarity. Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein By similarity. Cytoplasmic vesiclesecretory vesicle membrane; Single-pass type I membrane protein By similarity. Cell membrane By similarity. Golgi apparatustrans-Golgi network membrane; Single-pass type I membrane protein
Note: Cycles between compartments of the early secretatory pathway By similarity. Is one of the most abundant proteins in the cis-Golgi network. Detected in trans-Golgi network-derived transport vesicles.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini32 – 185154Lumenal Reviewed predictionAdd
BLAST
Transmembranei186 – 20621Helical; Reviewed predictionAdd
BLAST
Topological domaini207 – 21913Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. COPI-coated vesicle Source: UniProtKB
  2. endoplasmic reticulum-Golgi intermediate compartment membrane Source: UniProtKB-SubCell
  3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  4. gamma-secretase complex Source: UniProtKB
  5. Golgi membrane Source: GOC
  6. integral component of membrane Source: UniProtKB-KW
  7. melanosome Source: UniProtKB-SubCell
  8. plasma membrane Source: UniProtKB
  9. trans-Golgi network transport vesicle Source: UniProtKB
  10. transport vesicle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131 By similarityAdd
BLAST
Chaini32 – 219188Transmembrane protein Tmp21PRO_0000010400Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei171 – 1711Omega-N-methylated arginine By similarity
Modified residuei176 – 1761Omega-N-methylated arginine By similarity
Glycosylationi179 – 1791N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein, Methylation

Interactioni

Subunit structurei

Predominantly homodimeric and to lesser extent monomeric in endoplasmic reticulum. Homodimer and monomer in endoplasmic reticulum-Golgi intermediate compartment and cis-Golgi network. Probably oligomerizes with other members of the EMP24/GP25L family such as TMED2, TMED7 and TMED9. Interacts with TMED2. Associates with the COPI vesicle coat (coatomer); TMED10:TMED2 heterotetramers are proposed to be involved in coatomer association. Interacts (via C-terminus) with COPG1; the interaction involves dimeric TMED10. Interacts with ARF1 (GDP-bound); the interaction probably involves a TMED10 oligomer. Interacts with SEC23A; indicative for an association of TMED10 with the COPII vesicle coat. Interacts with CD59, SEC24B, SEC24C and SEC24D. Interacts with MPPE1/PGAP5. Interacts with F2LR1. Interacts with KDELR2; the interaction is disrupted by KDELR2 ligand. Found in a complex composed at least of SURF4, TMED2 and TMED10. Associates with the presenilin-dependent gamma-secretase complex By similarity. Interacts with STX17; the interaction is direct By similarity.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CD59P139875EBI-4405327,EBI-297972From a different organism.
TMED2Q153632EBI-4405327,EBI-998485From a different organism.

Protein-protein interaction databases

IntActiO35587. 6 interactions.
MINTiMINT-4996182.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 193153GOLDAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 142142Required for interaction with STX17 By similarityAdd
BLAST
Regioni147 – 17832Required for TMED10 and TMED2 cis-Golgi network localization By similarityAdd
BLAST
Regioni204 – 21916Interaction with COPG1 By similarityAdd
BLAST
Regioni207 – 21913Interaction with ARF1 By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi211 – 2199COPI vesicle coat-binding Reviewed prediction
Motifi211 – 2122COPII vesicle coat-binding Reviewed prediction

Domaini

The lumenal domain mediates localization to the plasma membrane by partially overriding the ER retention by the cytoplasmic domain By similarity.

Sequence similaritiesi

Belongs to the EMP24/GP25L family.
Contains 1 GOLD domain.

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG107275.

Family and domain databases

InterProiIPR009038. GOLD.
IPR015720. TMP21-related.
[Graphical view]
PANTHERiPTHR22811. PTHR22811. 1 hit.
PfamiPF01105. EMP24_GP25L. 1 hit.
[Graphical view]
PROSITEiPS50866. GOLD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35587-1 [UniParc]FASTAAdd to Basket

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MSGSSGPLSW PGPRPCALLF LLLLGPSSVL AISFHLPVNS RKCLREEIHK    50
DLLVTGAYEI TDQSGGAGGL RTHLKITDSA GHILYAKEDA TKGKFAFTTE 100
DYDMFEVCFE SKGTGRIPDQ LVILDMKHGV EAKNYEEIAK VEKLKPLEVE 150
LRRLEDLSES IVNDFAYMKK REEEMRDTNE STNTRVLYFS IFSMLCLIGL 200
ATWQVFYLRR FFKAKKLIE 219
Length:219
Mass (Da):24,821
Last modified:January 1, 1998 - v1
Checksum:i1FCF2E49ABB565EB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ001513 mRNA. Translation: CAA04796.1.
RefSeqiNP_001268617.1. NM_001281688.1.

Genome annotation databases

GeneIDi101832157.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ001513 mRNA. Translation: CAA04796.1 .
RefSeqi NP_001268617.1. NM_001281688.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O35587. 6 interactions.
MINTi MINT-4996182.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 101832157.

Phylogenomic databases

HOVERGENi HBG107275.

Family and domain databases

InterProi IPR009038. GOLD.
IPR015720. TMP21-related.
[Graphical view ]
PANTHERi PTHR22811. PTHR22811. 1 hit.
Pfami PF01105. EMP24_GP25L. 1 hit.
[Graphical view ]
PROSITEi PS50866. GOLD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Involvement of the transmembrane protein p23 in biosynthetic protein transport."
    Rojo M., Pepperkok R., Emery G., Kellner R., Stang E., Parton R.G., Gruenberg J.
    J. Cell Biol. 139:1119-1135(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Identification of components of trans-Golgi network-derived transport vesicles and detergent-insoluble complexes by nanoelectrospray tandem mass spectrometry."
    Shevchenko A., Keller P., Scheiffele P., Mann M., Simons K.
    Electrophoresis 18:2591-2600(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  3. "Selective export of human GPI-anchored proteins from the endoplasmic reticulum."
    Bonnon C., Wendeler M.W., Paccaud J.P., Hauri H.P.
    J. Cell Sci. 123:1705-1715(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CD59; SEC24B; SEC24C AND SEC24D.

Entry informationi

Entry nameiTMEDA_MESAU
AccessioniPrimary (citable) accession number: O35587
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: June 11, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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