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O35568 (FBLN3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
EGF-containing fibulin-like extracellular matrix protein 1
Alternative name(s):
Fibulin-3
Short name=FIBL-3
T16 protein
Gene names
Name:Efemp1
Synonyms:Fbln3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length493 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth. Ref.2

Subunit structure

Interacts with ECM1 By similarity. Interacts with TIMP3 By similarity.

Subcellular location

Secreted › extracellular space. Secreted › extracellular space › extracellular matrix. Note: Localizes to the lamina propria underneath the olfactory epithelium. Ref.2

Tissue specificity

Expressed by olfactory ensheathing cells (at protein level). Detected in lung, intestine and kidney. Ref.1

Sequence similarities

Belongs to the fibulin family.

Contains 6 EGF-like domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Chain18 – 493476EGF-containing fibulin-like extracellular matrix protein 1
PRO_0000007573

Regions

Domain26 – 7146EGF-like 1; atypical
Domain173 – 21341EGF-like 2; calcium-binding Potential
Domain214 – 25340EGF-like 3; calcium-binding Potential
Domain254 – 29340EGF-like 4; calcium-binding Potential
Domain294 – 33340EGF-like 5; calcium-binding Potential
Domain334 – 37845EGF-like 6; calcium-binding Potential
Region259 – 493235Mediates interaction with TIMP3 By similarity

Amino acid modifications

Glycosylation2491N-linked (GlcNAc...) Potential
Disulfide bond177 ↔ 190 By similarity
Disulfide bond184 ↔ 199 By similarity
Disulfide bond201 ↔ 212 By similarity
Disulfide bond218 ↔ 228 By similarity
Disulfide bond224 ↔ 237 By similarity
Disulfide bond239 ↔ 252 By similarity
Disulfide bond258 ↔ 268 By similarity
Disulfide bond264 ↔ 277 By similarity
Disulfide bond279 ↔ 292 By similarity
Disulfide bond298 ↔ 309 By similarity
Disulfide bond305 ↔ 318 By similarity
Disulfide bond320 ↔ 332 By similarity
Disulfide bond338 ↔ 350 By similarity
Disulfide bond344 ↔ 359 By similarity
Disulfide bond365 ↔ 377 By similarity

Sequences

Sequence LengthMass (Da)Tools
O35568 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 22DAFD70BACF1CA5

FASTA49354,596
        10         20         30         40         50         60 
MLQTVFLTML TLALVKSQVT EETITYTQCT DGYEWDPVRQ QCKDIDECDI VPDACKGGMK 

        70         80         90        100        110        120 
CVNHYGGYLC LPKTAQIIVN NEQPQQETPA AEASSGAATG TIAARSMATS GVIPGGGFIA 

       130        140        150        160        170        180 
SATAVAGPEV QTGRNNFVIR RNPADPQRIP SNPSHRIQCA AGYEQSEHNV CQDIDECTSG 

       190        200        210        220        230        240 
THNCRLDQVC INLRGSFTCH CLPGYQKRGE QCVDIDECSV PPYCHQGCVN TPGSFYCQCN 

       250        260        270        280        290        300 
PGFQLAANNY TCVDINECDA SNQCAQQCYN ILGSFICQCN QGYELSSDRL NCEDIDECRT 

       310        320        330        340        350        360 
SSYLCQYQCV NEPGKFSCMC PQGYQVVRSR TCQDINECET TNECREDEMC WNYHGGFRCY 

       370        380        390        400        410        420 
PQNPCQDPYV LTSENRCVCP VSNTMCRDVP QSIVYKYMNI RSDRSVPSDI FQIQATTIYA 

       430        440        450        460        470        480 
NTINTFRIKS GNENGEFYLR QTSPVSAMLV LVKSLTGPRE HIVGLEMLTV SSIGTFRTSS 

       490 
VLRLTIIVGP FSF

« Hide

References

[1]"Interaction of DA41, a DAN-binding protein, with the epidermal growth factor-like protein, S(1-5)."
Ozaki T., Kondo K., Nakamura Y., Ichimiya S., Nakagawara A., Sakiyama S.
Biochem. Biophys. Res. Commun. 237:245-250(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Lung.
[2]"The glycoprotein fibulin-3 regulates morphology and motility of olfactory ensheathing cells in vitro."
Vukovic J., Ruitenberg M.J., Roet K., Franssen E., Arulpragasam A., Sasaki T., Verhaagen J., Harvey A.R., Busfield S.J., Plant G.W.
Glia 57:424-443(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D89730 mRNA. Translation: BAA22265.1.
IPIIPI00365221.
PIRJC5621.
UniGeneRn.163265.

3D structure databases

ProteinModelPortalO35568.
ModBaseSearch...

PTM databases

PhosphoSiteO35568.

Proteomic databases

PaxDbO35568.
PRIDEO35568.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:1308528. rat.

Organism-specific databases

RGD1308528. Efemp1.

Phylogenomic databases

eggNOGNOG250502.
HOGENOMHOG000234337.
HOVERGENHBG051560.
InParanoidO35568.

Gene expression databases

ArrayExpressO35568.
GenevestigatorO35568.
GermOnlineENSRNOG00000003553. Rattus norvegicus.

Family and domain databases

InterProIPR026823. cEGF.
IPR026824. Efemp1/Efemp2.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
[Graphical view]
PANTHERPTHR24048:SF2. PTHR24048:SF2. 1 hit.
PfamPF12662. cEGF. 3 hits.
PF07645. EGF_CA. 2 hits.
[Graphical view]
SMARTSM00179. EGF_CA. 4 hits.
[Graphical view]
PROSITEPS00010. ASX_HYDROXYL. 4 hits.
PS00022. EGF_1. False negative.
PS01186. EGF_2. 4 hits.
PS50026. EGF_3. 4 hits.
PS01187. EGF_CA. 6 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFBLN3_RAT
AccessionPrimary (citable) accession number: O35568
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: April 3, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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