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Protein

6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3

Gene

Pfkfb3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Synthesis and degradation of fructose 2,6-bisphosphate.

Catalytic activityi

Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.
ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei75Fructose 6-phosphateBy similarity1
Binding sitei99Fructose 6-phosphateBy similarity1
Active sitei125Sequence analysis1
Binding sitei127Fructose 6-phosphateBy similarity1
Binding sitei133Fructose 6-phosphateBy similarity1
Active sitei155Sequence analysis1
Binding sitei169Fructose 6-phosphateBy similarity1
Binding sitei190Fructose 6-phosphateBy similarity1
Binding sitei194Fructose 6-phosphateBy similarity1
Binding sitei253Fructose 2,6-bisphosphateBy similarity1
Sitei253Transition state stabilizerBy similarity1
Active sitei254Tele-phosphohistidine intermediateBy similarity1
Binding sitei260Fructose 2,6-bisphosphateBy similarity1
Sitei260Transition state stabilizerBy similarity1
Binding sitei266Fructose 2,6-bisphosphate; via amide nitrogenBy similarity1
Active sitei323Proton donor/acceptorBy similarity1
Binding sitei334Fructose 2,6-bisphosphateBy similarity1
Binding sitei348Fructose 2,6-bisphosphateBy similarity1
Binding sitei352Fructose 2,6-bisphosphateBy similarity1
Binding sitei363Fructose 2,6-bisphosphateBy similarity1
Sitei388Transition state stabilizerBy similarity1
Binding sitei389Fructose 2,6-bisphosphateBy similarity1
Binding sitei393Fructose 2,6-bisphosphateBy similarity1
Binding sitei425ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi42 – 50ATPBy similarity9
Nucleotide bindingi164 – 169ATPBy similarity6
Nucleotide bindingi345 – 348ATPBy similarity4
Nucleotide bindingi389 – 393ATPBy similarity5

GO - Molecular functioni

  • 6-phosphofructo-2-kinase activity Source: UniProtKB-EC
  • ATP binding Source: UniProtKB-KW
  • fructose-2,6-bisphosphate 2-phosphatase activity Source: RGD

GO - Biological processi

  • brain development Source: RGD
  • fructose 2,6-bisphosphate metabolic process Source: RGD
  • fructose metabolic process Source: InterPro
  • regulation of glycolytic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.1.3.46. 5301.
ReactomeiR-RNO-70171. Glycolysis.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
Short name:
6PF-2-K/Fru-2,6-P2ase 3
Short name:
PFK/FBPase 3
Alternative name(s):
6PF-2-K/Fru-2,6-P2ase brain-type isozyme
RB2K
Including the following 2 domains:
Fructose-2,6-bisphosphatase (EC:3.1.3.46)
Gene namesi
Name:Pfkfb3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi619776. Pfkfb3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001799691 – 5556-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3Add BLAST555

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei490Phosphoserine; by AMPK and PKABy similarity1
Modified residuei492PhosphothreonineBy similarity1
Modified residuei496PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation by AMPK stimulates activity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO35552.
PRIDEiO35552.

PTM databases

iPTMnetiO35552.
PhosphoSitePlusiO35552.

Expressioni

Gene expression databases

BgeeiENSRNOG00000018911.
ExpressionAtlasiO35552. baseline and differential.
GenevisibleiO35552. RN.

Interactioni

Protein-protein interaction databases

IntActiO35552. 2 interactors.
STRINGi10116.ENSRNOP00000062965.

Structurei

3D structure databases

ProteinModelPortaliO35552.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 2456-phosphofructo-2-kinaseAdd BLAST245
Regioni246 – 555Fructose-2,6-bisphosphataseAdd BLAST310

Sequence similaritiesi

In the C-terminal section; belongs to the phosphoglycerate mutase family.Curated

Phylogenomic databases

eggNOGiKOG0234. Eukaryota.
COG0406. LUCA.
GeneTreeiENSGT00390000018751.
HOVERGENiHBG005628.
InParanoidiO35552.
KOiK01103.
OMAiADEMPYL.
OrthoDBiEOG091G0A43.
PhylomeDBiO35552.
TreeFamiTF313541.

Family and domain databases

CDDicd07067. HP_PGM_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003094. 6Pfruct_kin.
IPR013079. 6Phosfructo_kin.
IPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR027417. P-loop_NTPase.
IPR001345. PG/BPGM_mutase_AS.
[Graphical view]
PANTHERiPTHR10606. PTHR10606. 2 hits.
PfamiPF01591. 6PF2K. 1 hit.
PF00300. His_Phos_1. 1 hit.
[Graphical view]
PRINTSiPR00991. 6PFRUCTKNASE.
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF53254. SSF53254. 1 hit.
PROSITEiPS00175. PG_MUTASE. 1 hit.
[Graphical view]

Sequences (8)i

Sequence statusi: Complete.

This entry describes 8 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O35552-1) [UniParc]FASTAAdd to basket
Also known as: RB2K1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPLELTQSRV QKIWVPVDHR PSLPRSCGPK LTNSPTVIVM VGLPARGKTY
60 70 80 90 100
ISKKLTRYLN WIGVPTKVFN VGEYRREAVK QYSSYNFFRP DNEEAMRVRK
110 120 130 140 150
QCALAALRDV KSYLTKEGGQ IAVFDATNTT RERRHMILHF AKENDFKAFF
160 170 180 190 200
IESVCDDPTV VASNIMEVKI SSPDYKDCNS AEAMDDFMKR INCYEASYQP
210 220 230 240 250
LDPDKCDRDL SFIKVIDVGR RFLVNRVQDH IQSRIVYYLM NIHVQPRTIY
260 270 280 290 300
LCRHGENEYN VQGKIGGDSG LSSRGKKFAN ALSKFVEEQN LKDLRVWTSQ
310 320 330 340 350
LKSTIQTAEA LRLPYEQWKA LNEIDAGVCE ELTYEEIRDT YPEEYALREQ
360 370 380 390 400
DKYYYRYPTG ESYQDLVQRL EPVIMELERQ ENVLVICHQA VLRCLLAYFL
410 420 430 440 450
DKSAEEMPYL KCPLHTVLKL TPVAYGCRVE SIYLNVESVS THRERSEAVK
460 470 480 490 500
IQHFASVVRP SSYTELDFLS VESAKQDAKK GPNPLMRRNS VTPLASPEPT
510 520 530 540 550
KKPRINSFEE HVASTSAALP SCLPPEVPTQ LPGQPLLGKA CLRTVCHIFS

KFSPY
Length:555
Mass (Da):63,676
Last modified:January 1, 1998 - v1
Checksum:i45B2D090B44FCD8D
GO
Isoform 2 (identifier: O35552-2) [UniParc]FASTAAdd to basket
Also known as: RB2K2

The sequence of this isoform differs from the canonical sequence as follows:
     543-555: RTVCHIFSKFSPY → T

Show »
Length:543
Mass (Da):62,210
Checksum:iDA1B3F86851815D1
GO
Isoform 3 (identifier: O35552-3) [UniParc]FASTAAdd to basket
Also known as: RB2K3

The sequence of this isoform differs from the canonical sequence as follows:
     535-555: PLLGKACLRTVCHIFSKFSPY → NMRSPRSGAESSQKH

Show »
Length:549
Mass (Da):62,967
Checksum:i26E9DB0564825A82
GO
Isoform 4 (identifier: O35552-4) [UniParc]FASTAAdd to basket
Also known as: RB2K4

The sequence of this isoform differs from the canonical sequence as follows:
     448-476: Missing.

Show »
Length:526
Mass (Da):60,456
Checksum:iB41B90799C6003C9
GO
Isoform 5 (identifier: O35552-5) [UniParc]FASTAAdd to basket
Also known as: RB2K5

The sequence of this isoform differs from the canonical sequence as follows:
     448-476: Missing.
     543-555: RTVCHIFSKFSPY → T

Show »
Length:514
Mass (Da):58,991
Checksum:i6D3458ECED6716FC
GO
Isoform 6 (identifier: O35552-6) [UniParc]FASTAAdd to basket
Also known as: RB2K6

The sequence of this isoform differs from the canonical sequence as follows:
     448-476: Missing.
     535-555: PLLGKACLRTVCHIFSKFSPY → NMRSPRSGAESSQKH

Show »
Length:520
Mass (Da):59,747
Checksum:i78AD6CCDF66E1DAD
GO
Isoform 7 (identifier: O35552-7) [UniParc]FASTAAdd to basket
Also known as: RB2K7

The sequence of this isoform differs from the canonical sequence as follows:
     543-555: Missing.

Show »
Length:542
Mass (Da):62,109
Checksum:iCB3F86851815D1D7
GO
Isoform 8 (identifier: O35552-8) [UniParc]FASTAAdd to basket
Also known as: RB2K8

The sequence of this isoform differs from the canonical sequence as follows:
     448-476: Missing.
     543-555: Missing.

Show »
Length:513
Mass (Da):58,889
Checksum:i8458ECED6716FC15
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti185D → H in BAA22048 (Ref. 2) Curated1
Sequence conflicti367V → L in BAA22048 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004681448 – 476Missing in isoform 4, isoform 5, isoform 6 and isoform 8. CuratedAdd BLAST29
Alternative sequenceiVSP_004683535 – 555PLLGK…KFSPY → NMRSPRSGAESSQKH in isoform 3 and isoform 6. CuratedAdd BLAST21
Alternative sequenceiVSP_004682543 – 555RTVCH…KFSPY → T in isoform 2 and isoform 5. CuratedAdd BLAST13
Alternative sequenceiVSP_004684543 – 555Missing in isoform 7 and isoform 8. CuratedAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87240 mRNA. Translation: BAA21749.1.
D87241 mRNA. Translation: BAA21750.1.
D87242 mRNA. Translation: BAA21751.1.
D87243 mRNA. Translation: BAA21752.1.
D87244 mRNA. Translation: BAA21753.1.
D87245 mRNA. Translation: BAA21754.1.
D87246 mRNA. Translation: BAA21755.1.
D87247 mRNA. Translation: BAA21756.1.
AB006710 mRNA. Translation: BAA22048.1.
RefSeqiNP_476476.1. NM_057135.1. [O35552-1]
XP_006254254.1. XM_006254192.3. [O35552-3]
XP_006254255.1. XM_006254193.3. [O35552-2]
XP_006254258.1. XM_006254196.3. [O35552-4]
XP_006254259.1. XM_006254197.3. [O35552-6]
XP_006254260.1. XM_006254198.3. [O35552-5]
UniGeneiRn.10791.

Genome annotation databases

EnsembliENSRNOT00000025731; ENSRNOP00000025731; ENSRNOG00000018911. [O35552-8]
ENSRNOT00000051067; ENSRNOP00000040928; ENSRNOG00000018911. [O35552-2]
ENSRNOT00000068354; ENSRNOP00000062965; ENSRNOG00000018911. [O35552-1]
GeneIDi117276.
KEGGirno:117276.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87240 mRNA. Translation: BAA21749.1.
D87241 mRNA. Translation: BAA21750.1.
D87242 mRNA. Translation: BAA21751.1.
D87243 mRNA. Translation: BAA21752.1.
D87244 mRNA. Translation: BAA21753.1.
D87245 mRNA. Translation: BAA21754.1.
D87246 mRNA. Translation: BAA21755.1.
D87247 mRNA. Translation: BAA21756.1.
AB006710 mRNA. Translation: BAA22048.1.
RefSeqiNP_476476.1. NM_057135.1. [O35552-1]
XP_006254254.1. XM_006254192.3. [O35552-3]
XP_006254255.1. XM_006254193.3. [O35552-2]
XP_006254258.1. XM_006254196.3. [O35552-4]
XP_006254259.1. XM_006254197.3. [O35552-6]
XP_006254260.1. XM_006254198.3. [O35552-5]
UniGeneiRn.10791.

3D structure databases

ProteinModelPortaliO35552.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO35552. 2 interactors.
STRINGi10116.ENSRNOP00000062965.

PTM databases

iPTMnetiO35552.
PhosphoSitePlusiO35552.

Proteomic databases

PaxDbiO35552.
PRIDEiO35552.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000025731; ENSRNOP00000025731; ENSRNOG00000018911. [O35552-8]
ENSRNOT00000051067; ENSRNOP00000040928; ENSRNOG00000018911. [O35552-2]
ENSRNOT00000068354; ENSRNOP00000062965; ENSRNOG00000018911. [O35552-1]
GeneIDi117276.
KEGGirno:117276.

Organism-specific databases

CTDi5209.
RGDi619776. Pfkfb3.

Phylogenomic databases

eggNOGiKOG0234. Eukaryota.
COG0406. LUCA.
GeneTreeiENSGT00390000018751.
HOVERGENiHBG005628.
InParanoidiO35552.
KOiK01103.
OMAiADEMPYL.
OrthoDBiEOG091G0A43.
PhylomeDBiO35552.
TreeFamiTF313541.

Enzyme and pathway databases

BRENDAi3.1.3.46. 5301.
ReactomeiR-RNO-70171. Glycolysis.

Miscellaneous databases

PROiO35552.

Gene expression databases

BgeeiENSRNOG00000018911.
ExpressionAtlasiO35552. baseline and differential.
GenevisibleiO35552. RN.

Family and domain databases

CDDicd07067. HP_PGM_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003094. 6Pfruct_kin.
IPR013079. 6Phosfructo_kin.
IPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR027417. P-loop_NTPase.
IPR001345. PG/BPGM_mutase_AS.
[Graphical view]
PANTHERiPTHR10606. PTHR10606. 2 hits.
PfamiPF01591. 6PF2K. 1 hit.
PF00300. His_Phos_1. 1 hit.
[Graphical view]
PRINTSiPR00991. 6PFRUCTKNASE.
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF53254. SSF53254. 1 hit.
PROSITEiPS00175. PG_MUTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiF263_RAT
AccessioniPrimary (citable) accession number: O35552
Secondary accession number(s): O35096
, O35553, O35554, O35555, O35556, O35557, Q9QWQ5, Q9QWQ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.