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O35552

- F263_RAT

UniProt

O35552 - F263_RAT

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Protein
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
Gene
Pfkfb3
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Synthesis and degradation of fructose 2,6-bisphosphate.

Catalytic activityi

Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.
ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei75 – 751Fructose 6-phosphate By similarity
Binding sitei99 – 991Fructose 6-phosphate By similarity
Active sitei125 – 1251 Reviewed prediction
Binding sitei127 – 1271Fructose 6-phosphate By similarity
Binding sitei133 – 1331Fructose 6-phosphate By similarity
Active sitei155 – 1551 Reviewed prediction
Binding sitei169 – 1691Fructose 6-phosphate By similarity
Binding sitei190 – 1901Fructose 6-phosphate By similarity
Binding sitei194 – 1941Fructose 6-phosphate By similarity
Binding sitei253 – 2531Fructose 2,6-bisphosphate By similarity
Active sitei254 – 2541Tele-phosphohistidine intermediate
Binding sitei260 – 2601Fructose 2,6-bisphosphate By similarity
Binding sitei266 – 2661Fructose 2,6-bisphosphate; via amide nitrogen By similarity
Active sitei323 – 3231Proton donor/acceptor By similarity
Binding sitei334 – 3341Fructose 2,6-bisphosphate By similarity
Binding sitei348 – 3481Fructose 2,6-bisphosphate By similarity
Binding sitei352 – 3521Fructose 2,6-bisphosphate By similarity
Binding sitei363 – 3631Fructose 2,6-bisphosphate By similarity
Active sitei388 – 3881Proton donor/acceptor By similarity
Binding sitei389 – 3891Fructose 2,6-bisphosphate By similarity
Binding sitei393 – 3931Fructose 2,6-bisphosphate By similarity
Binding sitei425 – 4251ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi42 – 509ATP By similarity
Nucleotide bindingi164 – 1696ATP By similarity
Nucleotide bindingi345 – 3484ATP By similarity
Nucleotide bindingi389 – 3935ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructo-2-kinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. fructose-2,6-bisphosphate 2-phosphatase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. fructose 2,6-bisphosphate metabolic process Source: InterPro
  2. fructose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.1.3.46. 5301.
ReactomeiREACT_225694. Glycolysis.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
Short name:
6PF-2-K/Fru-2,6-P2ase 3
Short name:
PFK/FBPase 3
Alternative name(s):
6PF-2-K/Fru-2,6-P2ase brain-type isozyme
RB2K
Including the following 2 domains:
Fructose-2,6-bisphosphatase (EC:3.1.3.46)
Gene namesi
Name:Pfkfb3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 17

Organism-specific databases

RGDi619776. Pfkfb3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5555556-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
PRO_0000179969Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei490 – 4901Phosphoserine; by AMPK and PKA By similarity
Modified residuei492 – 4921Phosphothreonine By similarity
Modified residuei496 – 4961Phosphoserine By similarity

Post-translational modificationi

Phosphorylation by AMPK stimulates activity By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiO35552.

PTM databases

PhosphoSiteiO35552.

Expressioni

Gene expression databases

GenevestigatoriO35552.

Interactioni

Protein-protein interaction databases

IntActiO35552. 2 interactions.
STRINGi10116.ENSRNOP00000062965.

Structurei

3D structure databases

ProteinModelPortaliO35552.
SMRiO35552. Positions 3-446.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2452456-phosphofructo-2-kinase
Add
BLAST
Regioni246 – 555310Fructose-2,6-bisphosphatase
Add
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the phosphoglycerate mutase family.

Phylogenomic databases

eggNOGiCOG0406.
GeneTreeiENSGT00390000018751.
HOVERGENiHBG005628.
InParanoidiO35552.
KOiK01103.
OMAiADEMPYL.
OrthoDBiEOG7M3J03.
PhylomeDBiO35552.
TreeFamiTF313541.

Family and domain databases

Gene3Di3.40.50.1240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003094. 6Pfruct_kin.
IPR013079. 6Phosfructo_kin.
IPR016260. Bifunct_6PFK/fruc_bisP_Ptase.
IPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR027417. P-loop_NTPase.
IPR001345. PG/BPGM_mutase_AS.
[Graphical view]
PANTHERiPTHR10606. PTHR10606. 1 hit.
PfamiPF01591. 6PF2K. 1 hit.
PF00300. His_Phos_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000709. 6PFK_2-Ptase. 1 hit.
PRINTSiPR00991. 6PFRUCTKNASE.
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF53254. SSF53254. 1 hit.
PROSITEiPS00175. PG_MUTASE. 1 hit.
[Graphical view]

Sequences (8)i

Sequence statusi: Complete.

This entry describes 8 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O35552-1) [UniParc]FASTAAdd to Basket

Also known as: RB2K1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MPLELTQSRV QKIWVPVDHR PSLPRSCGPK LTNSPTVIVM VGLPARGKTY    50
ISKKLTRYLN WIGVPTKVFN VGEYRREAVK QYSSYNFFRP DNEEAMRVRK 100
QCALAALRDV KSYLTKEGGQ IAVFDATNTT RERRHMILHF AKENDFKAFF 150
IESVCDDPTV VASNIMEVKI SSPDYKDCNS AEAMDDFMKR INCYEASYQP 200
LDPDKCDRDL SFIKVIDVGR RFLVNRVQDH IQSRIVYYLM NIHVQPRTIY 250
LCRHGENEYN VQGKIGGDSG LSSRGKKFAN ALSKFVEEQN LKDLRVWTSQ 300
LKSTIQTAEA LRLPYEQWKA LNEIDAGVCE ELTYEEIRDT YPEEYALREQ 350
DKYYYRYPTG ESYQDLVQRL EPVIMELERQ ENVLVICHQA VLRCLLAYFL 400
DKSAEEMPYL KCPLHTVLKL TPVAYGCRVE SIYLNVESVS THRERSEAVK 450
IQHFASVVRP SSYTELDFLS VESAKQDAKK GPNPLMRRNS VTPLASPEPT 500
KKPRINSFEE HVASTSAALP SCLPPEVPTQ LPGQPLLGKA CLRTVCHIFS 550
KFSPY 555
Length:555
Mass (Da):63,676
Last modified:January 1, 1998 - v1
Checksum:i45B2D090B44FCD8D
GO
Isoform 2 (identifier: O35552-2) [UniParc]FASTAAdd to Basket

Also known as: RB2K2

The sequence of this isoform differs from the canonical sequence as follows:
     543-555: RTVCHIFSKFSPY → T

Show »
Length:543
Mass (Da):62,210
Checksum:iDA1B3F86851815D1
GO
Isoform 3 (identifier: O35552-3) [UniParc]FASTAAdd to Basket

Also known as: RB2K3

The sequence of this isoform differs from the canonical sequence as follows:
     535-555: PLLGKACLRTVCHIFSKFSPY → NMRSPRSGAESSQKH

Show »
Length:549
Mass (Da):62,967
Checksum:i26E9DB0564825A82
GO
Isoform 4 (identifier: O35552-4) [UniParc]FASTAAdd to Basket

Also known as: RB2K4

The sequence of this isoform differs from the canonical sequence as follows:
     448-476: Missing.

Show »
Length:526
Mass (Da):60,456
Checksum:iB41B90799C6003C9
GO
Isoform 5 (identifier: O35552-5) [UniParc]FASTAAdd to Basket

Also known as: RB2K5

The sequence of this isoform differs from the canonical sequence as follows:
     448-476: Missing.
     543-555: RTVCHIFSKFSPY → T

Show »
Length:514
Mass (Da):58,991
Checksum:i6D3458ECED6716FC
GO
Isoform 6 (identifier: O35552-6) [UniParc]FASTAAdd to Basket

Also known as: RB2K6

The sequence of this isoform differs from the canonical sequence as follows:
     448-476: Missing.
     535-555: PLLGKACLRTVCHIFSKFSPY → NMRSPRSGAESSQKH

Show »
Length:520
Mass (Da):59,747
Checksum:i78AD6CCDF66E1DAD
GO
Isoform 7 (identifier: O35552-7) [UniParc]FASTAAdd to Basket

Also known as: RB2K7

The sequence of this isoform differs from the canonical sequence as follows:
     543-555: Missing.

Show »
Length:542
Mass (Da):62,109
Checksum:iCB3F86851815D1D7
GO
Isoform 8 (identifier: O35552-8) [UniParc]FASTAAdd to Basket

Also known as: RB2K8

The sequence of this isoform differs from the canonical sequence as follows:
     448-476: Missing.
     543-555: Missing.

Show »
Length:513
Mass (Da):58,889
Checksum:i8458ECED6716FC15
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei448 – 47629Missing in isoform 4, isoform 5, isoform 6 and isoform 8.
VSP_004681Add
BLAST
Alternative sequencei535 – 55521PLLGK…KFSPY → NMRSPRSGAESSQKH in isoform 3 and isoform 6.
VSP_004683Add
BLAST
Alternative sequencei543 – 55513RTVCH…KFSPY → T in isoform 2 and isoform 5.
VSP_004682Add
BLAST
Alternative sequencei543 – 55513Missing in isoform 7 and isoform 8.
VSP_004684Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti185 – 1851D → H in BAA22048. 1 Publication
Sequence conflicti367 – 3671V → L in BAA22048. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D87240 mRNA. Translation: BAA21749.1.
D87241 mRNA. Translation: BAA21750.1.
D87242 mRNA. Translation: BAA21751.1.
D87243 mRNA. Translation: BAA21752.1.
D87244 mRNA. Translation: BAA21753.1.
D87245 mRNA. Translation: BAA21754.1.
D87246 mRNA. Translation: BAA21755.1.
D87247 mRNA. Translation: BAA21756.1.
AB006710 mRNA. Translation: BAA22048.1.
RefSeqiNP_476476.1. NM_057135.1. [O35552-1]
XP_006254254.1. XM_006254192.1. [O35552-3]
XP_006254255.1. XM_006254193.1. [O35552-2]
XP_006254257.1. XM_006254195.1. [O35552-4]
XP_006254258.1. XM_006254196.1. [O35552-4]
XP_006254259.1. XM_006254197.1. [O35552-6]
XP_006254260.1. XM_006254198.1. [O35552-5]
UniGeneiRn.10791.

Genome annotation databases

EnsembliENSRNOT00000025700; ENSRNOP00000025700; ENSRNOG00000018911. [O35552-3]
ENSRNOT00000025731; ENSRNOP00000025731; ENSRNOG00000018911. [O35552-2]
ENSRNOT00000051067; ENSRNOP00000040928; ENSRNOG00000018911. [O35552-4]
ENSRNOT00000068354; ENSRNOP00000062965; ENSRNOG00000018911. [O35552-1]
GeneIDi117276.
KEGGirno:117276.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D87240 mRNA. Translation: BAA21749.1 .
D87241 mRNA. Translation: BAA21750.1 .
D87242 mRNA. Translation: BAA21751.1 .
D87243 mRNA. Translation: BAA21752.1 .
D87244 mRNA. Translation: BAA21753.1 .
D87245 mRNA. Translation: BAA21754.1 .
D87246 mRNA. Translation: BAA21755.1 .
D87247 mRNA. Translation: BAA21756.1 .
AB006710 mRNA. Translation: BAA22048.1 .
RefSeqi NP_476476.1. NM_057135.1. [O35552-1 ]
XP_006254254.1. XM_006254192.1. [O35552-3 ]
XP_006254255.1. XM_006254193.1. [O35552-2 ]
XP_006254257.1. XM_006254195.1. [O35552-4 ]
XP_006254258.1. XM_006254196.1. [O35552-4 ]
XP_006254259.1. XM_006254197.1. [O35552-6 ]
XP_006254260.1. XM_006254198.1. [O35552-5 ]
UniGenei Rn.10791.

3D structure databases

ProteinModelPortali O35552.
SMRi O35552. Positions 3-446.
ModBasei Search...

Protein-protein interaction databases

IntActi O35552. 2 interactions.
STRINGi 10116.ENSRNOP00000062965.

PTM databases

PhosphoSitei O35552.

Proteomic databases

PRIDEi O35552.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000025700 ; ENSRNOP00000025700 ; ENSRNOG00000018911 . [O35552-3 ]
ENSRNOT00000025731 ; ENSRNOP00000025731 ; ENSRNOG00000018911 . [O35552-2 ]
ENSRNOT00000051067 ; ENSRNOP00000040928 ; ENSRNOG00000018911 . [O35552-4 ]
ENSRNOT00000068354 ; ENSRNOP00000062965 ; ENSRNOG00000018911 . [O35552-1 ]
GeneIDi 117276.
KEGGi rno:117276.

Organism-specific databases

CTDi 5209.
RGDi 619776. Pfkfb3.

Phylogenomic databases

eggNOGi COG0406.
GeneTreei ENSGT00390000018751.
HOVERGENi HBG005628.
InParanoidi O35552.
KOi K01103.
OMAi ADEMPYL.
OrthoDBi EOG7M3J03.
PhylomeDBi O35552.
TreeFami TF313541.

Enzyme and pathway databases

BRENDAi 3.1.3.46. 5301.
Reactomei REACT_225694. Glycolysis.

Miscellaneous databases

NextBioi 620213.
PROi O35552.

Gene expression databases

Genevestigatori O35552.

Family and domain databases

Gene3Di 3.40.50.1240. 1 hit.
3.40.50.300. 1 hit.
InterProi IPR003094. 6Pfruct_kin.
IPR013079. 6Phosfructo_kin.
IPR016260. Bifunct_6PFK/fruc_bisP_Ptase.
IPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR027417. P-loop_NTPase.
IPR001345. PG/BPGM_mutase_AS.
[Graphical view ]
PANTHERi PTHR10606. PTHR10606. 1 hit.
Pfami PF01591. 6PF2K. 1 hit.
PF00300. His_Phos_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000709. 6PFK_2-Ptase. 1 hit.
PRINTSi PR00991. 6PFRUCTKNASE.
SMARTi SM00855. PGAM. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
SSF53254. SSF53254. 1 hit.
PROSITEi PS00175. PG_MUTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Novel isoforms of rat brain fructose 6-phosphate 2-kinase/fructose 2,6-bisphosphatase are generated by tissue-specific alternative splicing."
    Watanabe F., Sakai A., Furuya E.
    J. Neurochem. 69:1-9(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. Sakakibara R.
    Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 61-369.
    Strain: Wistar.
    Tissue: Placenta.

Entry informationi

Entry nameiF263_RAT
AccessioniPrimary (citable) accession number: O35552
Secondary accession number(s): O35096
, O35553, O35554, O35555, O35556, O35557, Q9QWQ5, Q9QWQ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: January 1, 1998
Last modified: September 3, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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