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O35552

- F263_RAT

UniProt

O35552 - F263_RAT

Protein

6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3

Gene

Pfkfb3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Synthesis and degradation of fructose 2,6-bisphosphate.

    Catalytic activityi

    Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.
    ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei75 – 751Fructose 6-phosphateBy similarity
    Binding sitei99 – 991Fructose 6-phosphateBy similarity
    Active sitei125 – 1251Sequence Analysis
    Binding sitei127 – 1271Fructose 6-phosphateBy similarity
    Binding sitei133 – 1331Fructose 6-phosphateBy similarity
    Active sitei155 – 1551Sequence Analysis
    Binding sitei169 – 1691Fructose 6-phosphateBy similarity
    Binding sitei190 – 1901Fructose 6-phosphateBy similarity
    Binding sitei194 – 1941Fructose 6-phosphateBy similarity
    Binding sitei253 – 2531Fructose 2,6-bisphosphateBy similarity
    Active sitei254 – 2541Tele-phosphohistidine intermediate
    Binding sitei260 – 2601Fructose 2,6-bisphosphateBy similarity
    Binding sitei266 – 2661Fructose 2,6-bisphosphate; via amide nitrogenBy similarity
    Active sitei323 – 3231Proton donor/acceptorBy similarity
    Binding sitei334 – 3341Fructose 2,6-bisphosphateBy similarity
    Binding sitei348 – 3481Fructose 2,6-bisphosphateBy similarity
    Binding sitei352 – 3521Fructose 2,6-bisphosphateBy similarity
    Binding sitei363 – 3631Fructose 2,6-bisphosphateBy similarity
    Active sitei388 – 3881Proton donor/acceptorBy similarity
    Binding sitei389 – 3891Fructose 2,6-bisphosphateBy similarity
    Binding sitei393 – 3931Fructose 2,6-bisphosphateBy similarity
    Binding sitei425 – 4251ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi42 – 509ATPBy similarity
    Nucleotide bindingi164 – 1696ATPBy similarity
    Nucleotide bindingi345 – 3484ATPBy similarity
    Nucleotide bindingi389 – 3935ATPBy similarity

    GO - Molecular functioni

    1. 6-phosphofructo-2-kinase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW
    3. fructose-2,6-bisphosphate 2-phosphatase activity Source: RGD

    GO - Biological processi

    1. brain development Source: RGD
    2. dephosphorylation Source: GOC
    3. fructose 2,6-bisphosphate metabolic process Source: RGD
    4. fructose metabolic process Source: InterPro
    5. regulation of glycolytic process Source: RGD

    Keywords - Molecular functioni

    Hydrolase, Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi3.1.3.46. 5301.
    ReactomeiREACT_225694. Glycolysis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
    Short name:
    6PF-2-K/Fru-2,6-P2ase 3
    Short name:
    PFK/FBPase 3
    Alternative name(s):
    6PF-2-K/Fru-2,6-P2ase brain-type isozyme
    RB2K
    Including the following 2 domains:
    Fructose-2,6-bisphosphatase (EC:3.1.3.46)
    Gene namesi
    Name:Pfkfb3
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 17

    Organism-specific databases

    RGDi619776. Pfkfb3.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 5555556-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3PRO_0000179969Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei490 – 4901Phosphoserine; by AMPK and PKABy similarity
    Modified residuei492 – 4921PhosphothreonineBy similarity
    Modified residuei496 – 4961PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylation by AMPK stimulates activity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiO35552.

    PTM databases

    PhosphoSiteiO35552.

    Expressioni

    Gene expression databases

    GenevestigatoriO35552.

    Interactioni

    Protein-protein interaction databases

    IntActiO35552. 2 interactions.
    STRINGi10116.ENSRNOP00000062965.

    Structurei

    3D structure databases

    ProteinModelPortaliO35552.
    SMRiO35552. Positions 3-446.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 2452456-phosphofructo-2-kinaseAdd
    BLAST
    Regioni246 – 555310Fructose-2,6-bisphosphataseAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the phosphoglycerate mutase family.Curated

    Phylogenomic databases

    eggNOGiCOG0406.
    GeneTreeiENSGT00390000018751.
    HOVERGENiHBG005628.
    InParanoidiO35552.
    KOiK01103.
    OMAiADEMPYL.
    OrthoDBiEOG7M3J03.
    PhylomeDBiO35552.
    TreeFamiTF313541.

    Family and domain databases

    Gene3Di3.40.50.1240. 1 hit.
    3.40.50.300. 1 hit.
    InterProiIPR003094. 6Pfruct_kin.
    IPR013079. 6Phosfructo_kin.
    IPR016260. Bifunct_6PFK/fruc_bisP_Ptase.
    IPR013078. His_Pase_superF_clade-1.
    IPR029033. His_PPase_superfam.
    IPR027417. P-loop_NTPase.
    IPR001345. PG/BPGM_mutase_AS.
    [Graphical view]
    PANTHERiPTHR10606. PTHR10606. 1 hit.
    PfamiPF01591. 6PF2K. 1 hit.
    PF00300. His_Phos_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000709. 6PFK_2-Ptase. 1 hit.
    PRINTSiPR00991. 6PFRUCTKNASE.
    SMARTiSM00855. PGAM. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    SSF53254. SSF53254. 1 hit.
    PROSITEiPS00175. PG_MUTASE. 1 hit.
    [Graphical view]

    Sequences (8)i

    Sequence statusi: Complete.

    This entry describes 8 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O35552-1) [UniParc]FASTAAdd to Basket

    Also known as: RB2K1

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPLELTQSRV QKIWVPVDHR PSLPRSCGPK LTNSPTVIVM VGLPARGKTY    50
    ISKKLTRYLN WIGVPTKVFN VGEYRREAVK QYSSYNFFRP DNEEAMRVRK 100
    QCALAALRDV KSYLTKEGGQ IAVFDATNTT RERRHMILHF AKENDFKAFF 150
    IESVCDDPTV VASNIMEVKI SSPDYKDCNS AEAMDDFMKR INCYEASYQP 200
    LDPDKCDRDL SFIKVIDVGR RFLVNRVQDH IQSRIVYYLM NIHVQPRTIY 250
    LCRHGENEYN VQGKIGGDSG LSSRGKKFAN ALSKFVEEQN LKDLRVWTSQ 300
    LKSTIQTAEA LRLPYEQWKA LNEIDAGVCE ELTYEEIRDT YPEEYALREQ 350
    DKYYYRYPTG ESYQDLVQRL EPVIMELERQ ENVLVICHQA VLRCLLAYFL 400
    DKSAEEMPYL KCPLHTVLKL TPVAYGCRVE SIYLNVESVS THRERSEAVK 450
    IQHFASVVRP SSYTELDFLS VESAKQDAKK GPNPLMRRNS VTPLASPEPT 500
    KKPRINSFEE HVASTSAALP SCLPPEVPTQ LPGQPLLGKA CLRTVCHIFS 550
    KFSPY 555
    Length:555
    Mass (Da):63,676
    Last modified:January 1, 1998 - v1
    Checksum:i45B2D090B44FCD8D
    GO
    Isoform 2 (identifier: O35552-2) [UniParc]FASTAAdd to Basket

    Also known as: RB2K2

    The sequence of this isoform differs from the canonical sequence as follows:
         543-555: RTVCHIFSKFSPY → T

    Show »
    Length:543
    Mass (Da):62,210
    Checksum:iDA1B3F86851815D1
    GO
    Isoform 3 (identifier: O35552-3) [UniParc]FASTAAdd to Basket

    Also known as: RB2K3

    The sequence of this isoform differs from the canonical sequence as follows:
         535-555: PLLGKACLRTVCHIFSKFSPY → NMRSPRSGAESSQKH

    Show »
    Length:549
    Mass (Da):62,967
    Checksum:i26E9DB0564825A82
    GO
    Isoform 4 (identifier: O35552-4) [UniParc]FASTAAdd to Basket

    Also known as: RB2K4

    The sequence of this isoform differs from the canonical sequence as follows:
         448-476: Missing.

    Show »
    Length:526
    Mass (Da):60,456
    Checksum:iB41B90799C6003C9
    GO
    Isoform 5 (identifier: O35552-5) [UniParc]FASTAAdd to Basket

    Also known as: RB2K5

    The sequence of this isoform differs from the canonical sequence as follows:
         448-476: Missing.
         543-555: RTVCHIFSKFSPY → T

    Show »
    Length:514
    Mass (Da):58,991
    Checksum:i6D3458ECED6716FC
    GO
    Isoform 6 (identifier: O35552-6) [UniParc]FASTAAdd to Basket

    Also known as: RB2K6

    The sequence of this isoform differs from the canonical sequence as follows:
         448-476: Missing.
         535-555: PLLGKACLRTVCHIFSKFSPY → NMRSPRSGAESSQKH

    Show »
    Length:520
    Mass (Da):59,747
    Checksum:i78AD6CCDF66E1DAD
    GO
    Isoform 7 (identifier: O35552-7) [UniParc]FASTAAdd to Basket

    Also known as: RB2K7

    The sequence of this isoform differs from the canonical sequence as follows:
         543-555: Missing.

    Show »
    Length:542
    Mass (Da):62,109
    Checksum:iCB3F86851815D1D7
    GO
    Isoform 8 (identifier: O35552-8) [UniParc]FASTAAdd to Basket

    Also known as: RB2K8

    The sequence of this isoform differs from the canonical sequence as follows:
         448-476: Missing.
         543-555: Missing.

    Show »
    Length:513
    Mass (Da):58,889
    Checksum:i8458ECED6716FC15
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti185 – 1851D → H in BAA22048. 1 PublicationCurated
    Sequence conflicti367 – 3671V → L in BAA22048. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei448 – 47629Missing in isoform 4, isoform 5, isoform 6 and isoform 8. CuratedVSP_004681Add
    BLAST
    Alternative sequencei535 – 55521PLLGK…KFSPY → NMRSPRSGAESSQKH in isoform 3 and isoform 6. CuratedVSP_004683Add
    BLAST
    Alternative sequencei543 – 55513RTVCH…KFSPY → T in isoform 2 and isoform 5. CuratedVSP_004682Add
    BLAST
    Alternative sequencei543 – 55513Missing in isoform 7 and isoform 8. CuratedVSP_004684Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D87240 mRNA. Translation: BAA21749.1.
    D87241 mRNA. Translation: BAA21750.1.
    D87242 mRNA. Translation: BAA21751.1.
    D87243 mRNA. Translation: BAA21752.1.
    D87244 mRNA. Translation: BAA21753.1.
    D87245 mRNA. Translation: BAA21754.1.
    D87246 mRNA. Translation: BAA21755.1.
    D87247 mRNA. Translation: BAA21756.1.
    AB006710 mRNA. Translation: BAA22048.1.
    RefSeqiNP_476476.1. NM_057135.1. [O35552-1]
    XP_006254254.1. XM_006254192.1. [O35552-3]
    XP_006254255.1. XM_006254193.1. [O35552-2]
    XP_006254257.1. XM_006254195.1. [O35552-4]
    XP_006254258.1. XM_006254196.1. [O35552-4]
    XP_006254259.1. XM_006254197.1. [O35552-6]
    XP_006254260.1. XM_006254198.1. [O35552-5]
    UniGeneiRn.10791.

    Genome annotation databases

    EnsembliENSRNOT00000025700; ENSRNOP00000025700; ENSRNOG00000018911. [O35552-3]
    ENSRNOT00000025731; ENSRNOP00000025731; ENSRNOG00000018911. [O35552-2]
    ENSRNOT00000051067; ENSRNOP00000040928; ENSRNOG00000018911. [O35552-4]
    ENSRNOT00000068354; ENSRNOP00000062965; ENSRNOG00000018911. [O35552-1]
    GeneIDi117276.
    KEGGirno:117276.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D87240 mRNA. Translation: BAA21749.1 .
    D87241 mRNA. Translation: BAA21750.1 .
    D87242 mRNA. Translation: BAA21751.1 .
    D87243 mRNA. Translation: BAA21752.1 .
    D87244 mRNA. Translation: BAA21753.1 .
    D87245 mRNA. Translation: BAA21754.1 .
    D87246 mRNA. Translation: BAA21755.1 .
    D87247 mRNA. Translation: BAA21756.1 .
    AB006710 mRNA. Translation: BAA22048.1 .
    RefSeqi NP_476476.1. NM_057135.1. [O35552-1 ]
    XP_006254254.1. XM_006254192.1. [O35552-3 ]
    XP_006254255.1. XM_006254193.1. [O35552-2 ]
    XP_006254257.1. XM_006254195.1. [O35552-4 ]
    XP_006254258.1. XM_006254196.1. [O35552-4 ]
    XP_006254259.1. XM_006254197.1. [O35552-6 ]
    XP_006254260.1. XM_006254198.1. [O35552-5 ]
    UniGenei Rn.10791.

    3D structure databases

    ProteinModelPortali O35552.
    SMRi O35552. Positions 3-446.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O35552. 2 interactions.
    STRINGi 10116.ENSRNOP00000062965.

    PTM databases

    PhosphoSitei O35552.

    Proteomic databases

    PRIDEi O35552.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000025700 ; ENSRNOP00000025700 ; ENSRNOG00000018911 . [O35552-3 ]
    ENSRNOT00000025731 ; ENSRNOP00000025731 ; ENSRNOG00000018911 . [O35552-2 ]
    ENSRNOT00000051067 ; ENSRNOP00000040928 ; ENSRNOG00000018911 . [O35552-4 ]
    ENSRNOT00000068354 ; ENSRNOP00000062965 ; ENSRNOG00000018911 . [O35552-1 ]
    GeneIDi 117276.
    KEGGi rno:117276.

    Organism-specific databases

    CTDi 5209.
    RGDi 619776. Pfkfb3.

    Phylogenomic databases

    eggNOGi COG0406.
    GeneTreei ENSGT00390000018751.
    HOVERGENi HBG005628.
    InParanoidi O35552.
    KOi K01103.
    OMAi ADEMPYL.
    OrthoDBi EOG7M3J03.
    PhylomeDBi O35552.
    TreeFami TF313541.

    Enzyme and pathway databases

    BRENDAi 3.1.3.46. 5301.
    Reactomei REACT_225694. Glycolysis.

    Miscellaneous databases

    NextBioi 620213.
    PROi O35552.

    Gene expression databases

    Genevestigatori O35552.

    Family and domain databases

    Gene3Di 3.40.50.1240. 1 hit.
    3.40.50.300. 1 hit.
    InterProi IPR003094. 6Pfruct_kin.
    IPR013079. 6Phosfructo_kin.
    IPR016260. Bifunct_6PFK/fruc_bisP_Ptase.
    IPR013078. His_Pase_superF_clade-1.
    IPR029033. His_PPase_superfam.
    IPR027417. P-loop_NTPase.
    IPR001345. PG/BPGM_mutase_AS.
    [Graphical view ]
    PANTHERi PTHR10606. PTHR10606. 1 hit.
    Pfami PF01591. 6PF2K. 1 hit.
    PF00300. His_Phos_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000709. 6PFK_2-Ptase. 1 hit.
    PRINTSi PR00991. 6PFRUCTKNASE.
    SMARTi SM00855. PGAM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    SSF53254. SSF53254. 1 hit.
    PROSITEi PS00175. PG_MUTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Novel isoforms of rat brain fructose 6-phosphate 2-kinase/fructose 2,6-bisphosphatase are generated by tissue-specific alternative splicing."
      Watanabe F., Sakai A., Furuya E.
      J. Neurochem. 69:1-9(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
      Strain: Sprague-Dawley.
      Tissue: Brain.
    2. Sakakibara R.
      Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 61-369.
      Strain: Wistar.
      Tissue: Placenta.

    Entry informationi

    Entry nameiF263_RAT
    AccessioniPrimary (citable) accession number: O35552
    Secondary accession number(s): O35096
    , O35553, O35554, O35555, O35556, O35557, Q9QWQ5, Q9QWQ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 24, 2001
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3