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O35552 (F263_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3

Short name=6PF-2-K/Fru-2,6-P2ase 3
Short name=PFK/FBPase 3
Alternative name(s):
6PF-2-K/Fru-2,6-P2ase brain-type isozyme
RB2K

Including the following 2 domains:

  1. 6-phosphofructo-2-kinase
    EC=2.7.1.105
  2. Fructose-2,6-bisphosphatase
    EC=3.1.3.46
Gene names
Name:Pfkfb3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length555 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Synthesis and degradation of fructose 2,6-bisphosphate.

Catalytic activity

Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.

ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.

Post-translational modification

Phosphorylation by AMPK stimulates activity By similarity.

Sequence similarities

In the C-terminal section; belongs to the phosphoglycerate mutase family.

Alternative products

This entry describes 8 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O35552-1)

Also known as: RB2K1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O35552-2)

Also known as: RB2K2;

The sequence of this isoform differs from the canonical sequence as follows:
     543-555: RTVCHIFSKFSPY → T
Isoform 3 (identifier: O35552-3)

Also known as: RB2K3;

The sequence of this isoform differs from the canonical sequence as follows:
     535-555: PLLGKACLRTVCHIFSKFSPY → NMRSPRSGAESSQKH
Isoform 4 (identifier: O35552-4)

Also known as: RB2K4;

The sequence of this isoform differs from the canonical sequence as follows:
     448-476: Missing.
Isoform 5 (identifier: O35552-5)

Also known as: RB2K5;

The sequence of this isoform differs from the canonical sequence as follows:
     448-476: Missing.
     543-555: RTVCHIFSKFSPY → T
Isoform 6 (identifier: O35552-6)

Also known as: RB2K6;

The sequence of this isoform differs from the canonical sequence as follows:
     448-476: Missing.
     535-555: PLLGKACLRTVCHIFSKFSPY → NMRSPRSGAESSQKH
Isoform 7 (identifier: O35552-7)

Also known as: RB2K7;

The sequence of this isoform differs from the canonical sequence as follows:
     543-555: Missing.
Isoform 8 (identifier: O35552-8)

Also known as: RB2K8;

The sequence of this isoform differs from the canonical sequence as follows:
     448-476: Missing.
     543-555: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5555556-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
PRO_0000179969

Regions

Nucleotide binding42 – 509ATP By similarity
Nucleotide binding164 – 1696ATP By similarity
Nucleotide binding345 – 3484ATP By similarity
Nucleotide binding389 – 3935ATP By similarity
Region1 – 2452456-phosphofructo-2-kinase
Region246 – 555310Fructose-2,6-bisphosphatase

Sites

Active site1251 Potential
Active site1551 Potential
Active site2541Tele-phosphohistidine intermediate
Active site3231Proton donor/acceptor By similarity
Active site3881Proton donor/acceptor By similarity
Binding site751Fructose 6-phosphate By similarity
Binding site991Fructose 6-phosphate By similarity
Binding site1271Fructose 6-phosphate By similarity
Binding site1331Fructose 6-phosphate By similarity
Binding site1691Fructose 6-phosphate By similarity
Binding site1901Fructose 6-phosphate By similarity
Binding site1941Fructose 6-phosphate By similarity
Binding site2531Fructose 2,6-bisphosphate By similarity
Binding site2601Fructose 2,6-bisphosphate By similarity
Binding site2661Fructose 2,6-bisphosphate; via amide nitrogen By similarity
Binding site3341Fructose 2,6-bisphosphate By similarity
Binding site3481Fructose 2,6-bisphosphate By similarity
Binding site3521Fructose 2,6-bisphosphate By similarity
Binding site3631Fructose 2,6-bisphosphate By similarity
Binding site3891Fructose 2,6-bisphosphate By similarity
Binding site3931Fructose 2,6-bisphosphate By similarity
Binding site4251ATP By similarity

Amino acid modifications

Modified residue4901Phosphoserine; by AMPK and PKA By similarity
Modified residue4921Phosphothreonine By similarity
Modified residue4961Phosphoserine By similarity

Natural variations

Alternative sequence448 – 47629Missing in isoform 4, isoform 5, isoform 6 and isoform 8.
VSP_004681
Alternative sequence535 – 55521PLLGK…KFSPY → NMRSPRSGAESSQKH in isoform 3 and isoform 6.
VSP_004683
Alternative sequence543 – 55513RTVCH…KFSPY → T in isoform 2 and isoform 5.
VSP_004682
Alternative sequence543 – 55513Missing in isoform 7 and isoform 8.
VSP_004684

Experimental info

Sequence conflict1851D → H in BAA22048. Ref.2
Sequence conflict3671V → L in BAA22048. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (RB2K1) [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 45B2D090B44FCD8D

FASTA55563,676
        10         20         30         40         50         60 
MPLELTQSRV QKIWVPVDHR PSLPRSCGPK LTNSPTVIVM VGLPARGKTY ISKKLTRYLN 

        70         80         90        100        110        120 
WIGVPTKVFN VGEYRREAVK QYSSYNFFRP DNEEAMRVRK QCALAALRDV KSYLTKEGGQ 

       130        140        150        160        170        180 
IAVFDATNTT RERRHMILHF AKENDFKAFF IESVCDDPTV VASNIMEVKI SSPDYKDCNS 

       190        200        210        220        230        240 
AEAMDDFMKR INCYEASYQP LDPDKCDRDL SFIKVIDVGR RFLVNRVQDH IQSRIVYYLM 

       250        260        270        280        290        300 
NIHVQPRTIY LCRHGENEYN VQGKIGGDSG LSSRGKKFAN ALSKFVEEQN LKDLRVWTSQ 

       310        320        330        340        350        360 
LKSTIQTAEA LRLPYEQWKA LNEIDAGVCE ELTYEEIRDT YPEEYALREQ DKYYYRYPTG 

       370        380        390        400        410        420 
ESYQDLVQRL EPVIMELERQ ENVLVICHQA VLRCLLAYFL DKSAEEMPYL KCPLHTVLKL 

       430        440        450        460        470        480 
TPVAYGCRVE SIYLNVESVS THRERSEAVK IQHFASVVRP SSYTELDFLS VESAKQDAKK 

       490        500        510        520        530        540 
GPNPLMRRNS VTPLASPEPT KKPRINSFEE HVASTSAALP SCLPPEVPTQ LPGQPLLGKA 

       550 
CLRTVCHIFS KFSPY 

« Hide

Isoform 2 (RB2K2) [UniParc].

Checksum: DA1B3F86851815D1
Show »

FASTA54362,210
Isoform 3 (RB2K3) [UniParc].

Checksum: 26E9DB0564825A82
Show »

FASTA54962,967
Isoform 4 (RB2K4) [UniParc].

Checksum: B41B90799C6003C9
Show »

FASTA52660,456
Isoform 5 (RB2K5) [UniParc].

Checksum: 6D3458ECED6716FC
Show »

FASTA51458,991
Isoform 6 (RB2K6) [UniParc].

Checksum: 78AD6CCDF66E1DAD
Show »

FASTA52059,747
Isoform 7 (RB2K7) [UniParc].

Checksum: CB3F86851815D1D7
Show »

FASTA54262,109
Isoform 8 (RB2K8) [UniParc].

Checksum: 8458ECED6716FC15
Show »

FASTA51358,889

References

[1]"Novel isoforms of rat brain fructose 6-phosphate 2-kinase/fructose 2,6-bisphosphatase are generated by tissue-specific alternative splicing."
Watanabe F., Sakai A., Furuya E.
J. Neurochem. 69:1-9(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
Strain: Sprague-Dawley.
Tissue: Brain.
[2]Sakakibara R.
Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 61-369.
Strain: Wistar.
Tissue: Placenta.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D87240 mRNA. Translation: BAA21749.1.
D87241 mRNA. Translation: BAA21750.1.
D87242 mRNA. Translation: BAA21751.1.
D87243 mRNA. Translation: BAA21752.1.
D87244 mRNA. Translation: BAA21753.1.
D87245 mRNA. Translation: BAA21754.1.
D87246 mRNA. Translation: BAA21755.1.
D87247 mRNA. Translation: BAA21756.1.
AB006710 mRNA. Translation: BAA22048.1.
RefSeqNP_476476.1. NM_057135.1. [O35552-1]
XP_006254254.1. XM_006254192.1. [O35552-3]
XP_006254255.1. XM_006254193.1. [O35552-2]
XP_006254257.1. XM_006254195.1. [O35552-4]
XP_006254258.1. XM_006254196.1. [O35552-4]
XP_006254259.1. XM_006254197.1. [O35552-6]
XP_006254260.1. XM_006254198.1. [O35552-5]
UniGeneRn.10791.

3D structure databases

ProteinModelPortalO35552.
SMRO35552. Positions 3-446.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO35552. 2 interactions.
STRING10116.ENSRNOP00000062965.

PTM databases

PhosphoSiteO35552.

Proteomic databases

PRIDEO35552.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000025700; ENSRNOP00000025700; ENSRNOG00000018911. [O35552-3]
ENSRNOT00000025731; ENSRNOP00000025731; ENSRNOG00000018911. [O35552-2]
ENSRNOT00000051067; ENSRNOP00000040928; ENSRNOG00000018911. [O35552-4]
ENSRNOT00000068354; ENSRNOP00000062965; ENSRNOG00000018911. [O35552-1]
GeneID117276.
KEGGrno:117276.

Organism-specific databases

CTD5209.
RGD619776. Pfkfb3.

Phylogenomic databases

eggNOGCOG0406.
GeneTreeENSGT00390000018751.
HOVERGENHBG005628.
InParanoidO35552.
KOK01103.
OMAADEMPYL.
OrthoDBEOG7M3J03.
PhylomeDBO35552.
TreeFamTF313541.

Enzyme and pathway databases

BRENDA3.1.3.46. 5301.

Gene expression databases

GenevestigatorO35552.

Family and domain databases

Gene3D3.40.50.1240. 1 hit.
3.40.50.300. 1 hit.
InterProIPR003094. 6Pfruct_kin.
IPR013079. 6Phosfructo_kin.
IPR016260. Bifunct_6PFK/fruc_bisP_Ptase.
IPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR027417. P-loop_NTPase.
IPR001345. PG/BPGM_mutase_AS.
[Graphical view]
PANTHERPTHR10606. PTHR10606. 1 hit.
PfamPF01591. 6PF2K. 1 hit.
PF00300. His_Phos_1. 1 hit.
[Graphical view]
PIRSFPIRSF000709. 6PFK_2-Ptase. 1 hit.
PRINTSPR00991. 6PFRUCTKNASE.
SMARTSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
SSF53254. SSF53254. 1 hit.
PROSITEPS00175. PG_MUTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio620213.
PROO35552.

Entry information

Entry nameF263_RAT
AccessionPrimary (citable) accession number: O35552
Secondary accession number(s): O35096 expand/collapse secondary AC list , O35553, O35554, O35555, O35556, O35557, Q9QWQ5, Q9QWQ6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: January 1, 1998
Last modified: June 11, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families