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Protein

6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3

Gene

Pfkfb3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Synthesis and degradation of fructose 2,6-bisphosphate.

Catalytic activityi

Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.
ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei75 – 751Fructose 6-phosphateBy similarity
Binding sitei99 – 991Fructose 6-phosphateBy similarity
Active sitei125 – 1251Sequence Analysis
Binding sitei127 – 1271Fructose 6-phosphateBy similarity
Binding sitei133 – 1331Fructose 6-phosphateBy similarity
Active sitei155 – 1551Sequence Analysis
Binding sitei169 – 1691Fructose 6-phosphateBy similarity
Binding sitei190 – 1901Fructose 6-phosphateBy similarity
Binding sitei194 – 1941Fructose 6-phosphateBy similarity
Binding sitei253 – 2531Fructose 2,6-bisphosphateBy similarity
Active sitei254 – 2541Tele-phosphohistidine intermediate
Binding sitei260 – 2601Fructose 2,6-bisphosphateBy similarity
Binding sitei266 – 2661Fructose 2,6-bisphosphate; via amide nitrogenBy similarity
Active sitei323 – 3231Proton donor/acceptorBy similarity
Binding sitei334 – 3341Fructose 2,6-bisphosphateBy similarity
Binding sitei348 – 3481Fructose 2,6-bisphosphateBy similarity
Binding sitei352 – 3521Fructose 2,6-bisphosphateBy similarity
Binding sitei363 – 3631Fructose 2,6-bisphosphateBy similarity
Active sitei388 – 3881Proton donor/acceptorBy similarity
Binding sitei389 – 3891Fructose 2,6-bisphosphateBy similarity
Binding sitei393 – 3931Fructose 2,6-bisphosphateBy similarity
Binding sitei425 – 4251ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi42 – 509ATPBy similarity
Nucleotide bindingi164 – 1696ATPBy similarity
Nucleotide bindingi345 – 3484ATPBy similarity
Nucleotide bindingi389 – 3935ATPBy similarity

GO - Molecular functioni

  1. 6-phosphofructo-2-kinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. fructose-2,6-bisphosphate 2-phosphatase activity Source: RGD

GO - Biological processi

  1. brain development Source: RGD
  2. dephosphorylation Source: GOC
  3. fructose 2,6-bisphosphate metabolic process Source: RGD
  4. fructose metabolic process Source: InterPro
  5. regulation of glycolytic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.1.3.46. 5301.
ReactomeiREACT_225694. Glycolysis.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
Short name:
6PF-2-K/Fru-2,6-P2ase 3
Short name:
PFK/FBPase 3
Alternative name(s):
6PF-2-K/Fru-2,6-P2ase brain-type isozyme
RB2K
Including the following 2 domains:
Fructose-2,6-bisphosphatase (EC:3.1.3.46)
Gene namesi
Name:Pfkfb3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 17

Organism-specific databases

RGDi619776. Pfkfb3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5555556-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3PRO_0000179969Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei490 – 4901Phosphoserine; by AMPK and PKABy similarity
Modified residuei492 – 4921PhosphothreonineBy similarity
Modified residuei496 – 4961PhosphoserineBy similarity

Post-translational modificationi

Phosphorylation by AMPK stimulates activity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiO35552.

PTM databases

PhosphoSiteiO35552.

Expressioni

Gene expression databases

GenevestigatoriO35552.

Interactioni

Protein-protein interaction databases

IntActiO35552. 2 interactions.
STRINGi10116.ENSRNOP00000062965.

Structurei

3D structure databases

ProteinModelPortaliO35552.
SMRiO35552. Positions 3-446.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2452456-phosphofructo-2-kinaseAdd
BLAST
Regioni246 – 555310Fructose-2,6-bisphosphataseAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the phosphoglycerate mutase family.Curated

Phylogenomic databases

eggNOGiCOG0406.
GeneTreeiENSGT00390000018751.
HOVERGENiHBG005628.
InParanoidiO35552.
KOiK01103.
OMAiADEMPYL.
OrthoDBiEOG7M3J03.
PhylomeDBiO35552.
TreeFamiTF313541.

Family and domain databases

Gene3Di3.40.50.1240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003094. 6Pfruct_kin.
IPR013079. 6Phosfructo_kin.
IPR016260. Bifunct_6PFK/fruc_bisP_Ptase.
IPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR027417. P-loop_NTPase.
IPR001345. PG/BPGM_mutase_AS.
[Graphical view]
PANTHERiPTHR10606. PTHR10606. 1 hit.
PfamiPF01591. 6PF2K. 1 hit.
PF00300. His_Phos_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000709. 6PFK_2-Ptase. 1 hit.
PRINTSiPR00991. 6PFRUCTKNASE.
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF53254. SSF53254. 1 hit.
PROSITEiPS00175. PG_MUTASE. 1 hit.
[Graphical view]

Sequences (8)i

Sequence statusi: Complete.

This entry describes 8 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O35552-1) [UniParc]FASTAAdd to Basket

Also known as: RB2K1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPLELTQSRV QKIWVPVDHR PSLPRSCGPK LTNSPTVIVM VGLPARGKTY
60 70 80 90 100
ISKKLTRYLN WIGVPTKVFN VGEYRREAVK QYSSYNFFRP DNEEAMRVRK
110 120 130 140 150
QCALAALRDV KSYLTKEGGQ IAVFDATNTT RERRHMILHF AKENDFKAFF
160 170 180 190 200
IESVCDDPTV VASNIMEVKI SSPDYKDCNS AEAMDDFMKR INCYEASYQP
210 220 230 240 250
LDPDKCDRDL SFIKVIDVGR RFLVNRVQDH IQSRIVYYLM NIHVQPRTIY
260 270 280 290 300
LCRHGENEYN VQGKIGGDSG LSSRGKKFAN ALSKFVEEQN LKDLRVWTSQ
310 320 330 340 350
LKSTIQTAEA LRLPYEQWKA LNEIDAGVCE ELTYEEIRDT YPEEYALREQ
360 370 380 390 400
DKYYYRYPTG ESYQDLVQRL EPVIMELERQ ENVLVICHQA VLRCLLAYFL
410 420 430 440 450
DKSAEEMPYL KCPLHTVLKL TPVAYGCRVE SIYLNVESVS THRERSEAVK
460 470 480 490 500
IQHFASVVRP SSYTELDFLS VESAKQDAKK GPNPLMRRNS VTPLASPEPT
510 520 530 540 550
KKPRINSFEE HVASTSAALP SCLPPEVPTQ LPGQPLLGKA CLRTVCHIFS

KFSPY
Length:555
Mass (Da):63,676
Last modified:January 1, 1998 - v1
Checksum:i45B2D090B44FCD8D
GO
Isoform 2 (identifier: O35552-2) [UniParc]FASTAAdd to Basket

Also known as: RB2K2

The sequence of this isoform differs from the canonical sequence as follows:
     543-555: RTVCHIFSKFSPY → T

Show »
Length:543
Mass (Da):62,210
Checksum:iDA1B3F86851815D1
GO
Isoform 3 (identifier: O35552-3) [UniParc]FASTAAdd to Basket

Also known as: RB2K3

The sequence of this isoform differs from the canonical sequence as follows:
     535-555: PLLGKACLRTVCHIFSKFSPY → NMRSPRSGAESSQKH

Show »
Length:549
Mass (Da):62,967
Checksum:i26E9DB0564825A82
GO
Isoform 4 (identifier: O35552-4) [UniParc]FASTAAdd to Basket

Also known as: RB2K4

The sequence of this isoform differs from the canonical sequence as follows:
     448-476: Missing.

Show »
Length:526
Mass (Da):60,456
Checksum:iB41B90799C6003C9
GO
Isoform 5 (identifier: O35552-5) [UniParc]FASTAAdd to Basket

Also known as: RB2K5

The sequence of this isoform differs from the canonical sequence as follows:
     448-476: Missing.
     543-555: RTVCHIFSKFSPY → T

Show »
Length:514
Mass (Da):58,991
Checksum:i6D3458ECED6716FC
GO
Isoform 6 (identifier: O35552-6) [UniParc]FASTAAdd to Basket

Also known as: RB2K6

The sequence of this isoform differs from the canonical sequence as follows:
     448-476: Missing.
     535-555: PLLGKACLRTVCHIFSKFSPY → NMRSPRSGAESSQKH

Show »
Length:520
Mass (Da):59,747
Checksum:i78AD6CCDF66E1DAD
GO
Isoform 7 (identifier: O35552-7) [UniParc]FASTAAdd to Basket

Also known as: RB2K7

The sequence of this isoform differs from the canonical sequence as follows:
     543-555: Missing.

Show »
Length:542
Mass (Da):62,109
Checksum:iCB3F86851815D1D7
GO
Isoform 8 (identifier: O35552-8) [UniParc]FASTAAdd to Basket

Also known as: RB2K8

The sequence of this isoform differs from the canonical sequence as follows:
     448-476: Missing.
     543-555: Missing.

Show »
Length:513
Mass (Da):58,889
Checksum:i8458ECED6716FC15
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti185 – 1851D → H in BAA22048. 1 PublicationCurated
Sequence conflicti367 – 3671V → L in BAA22048. 1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei448 – 47629Missing in isoform 4, isoform 5, isoform 6 and isoform 8. CuratedVSP_004681Add
BLAST
Alternative sequencei535 – 55521PLLGK…KFSPY → NMRSPRSGAESSQKH in isoform 3 and isoform 6. CuratedVSP_004683Add
BLAST
Alternative sequencei543 – 55513RTVCH…KFSPY → T in isoform 2 and isoform 5. CuratedVSP_004682Add
BLAST
Alternative sequencei543 – 55513Missing in isoform 7 and isoform 8. CuratedVSP_004684Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87240 mRNA. Translation: BAA21749.1.
D87241 mRNA. Translation: BAA21750.1.
D87242 mRNA. Translation: BAA21751.1.
D87243 mRNA. Translation: BAA21752.1.
D87244 mRNA. Translation: BAA21753.1.
D87245 mRNA. Translation: BAA21754.1.
D87246 mRNA. Translation: BAA21755.1.
D87247 mRNA. Translation: BAA21756.1.
AB006710 mRNA. Translation: BAA22048.1.
RefSeqiNP_476476.1. NM_057135.1. [O35552-1]
XP_006254254.1. XM_006254192.2. [O35552-3]
XP_006254255.1. XM_006254193.2. [O35552-2]
XP_006254257.1. XM_006254195.2. [O35552-4]
XP_006254258.1. XM_006254196.2. [O35552-4]
XP_006254259.1. XM_006254197.2. [O35552-6]
XP_006254260.1. XM_006254198.2. [O35552-5]
XP_008770072.1. XM_008771850.1. [O35552-1]
UniGeneiRn.10791.

Genome annotation databases

EnsembliENSRNOT00000025700; ENSRNOP00000025700; ENSRNOG00000018911. [O35552-3]
ENSRNOT00000025731; ENSRNOP00000025731; ENSRNOG00000018911. [O35552-2]
ENSRNOT00000051067; ENSRNOP00000040928; ENSRNOG00000018911. [O35552-4]
ENSRNOT00000068354; ENSRNOP00000062965; ENSRNOG00000018911. [O35552-1]
GeneIDi117276.
KEGGirno:117276.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87240 mRNA. Translation: BAA21749.1.
D87241 mRNA. Translation: BAA21750.1.
D87242 mRNA. Translation: BAA21751.1.
D87243 mRNA. Translation: BAA21752.1.
D87244 mRNA. Translation: BAA21753.1.
D87245 mRNA. Translation: BAA21754.1.
D87246 mRNA. Translation: BAA21755.1.
D87247 mRNA. Translation: BAA21756.1.
AB006710 mRNA. Translation: BAA22048.1.
RefSeqiNP_476476.1. NM_057135.1. [O35552-1]
XP_006254254.1. XM_006254192.2. [O35552-3]
XP_006254255.1. XM_006254193.2. [O35552-2]
XP_006254257.1. XM_006254195.2. [O35552-4]
XP_006254258.1. XM_006254196.2. [O35552-4]
XP_006254259.1. XM_006254197.2. [O35552-6]
XP_006254260.1. XM_006254198.2. [O35552-5]
XP_008770072.1. XM_008771850.1. [O35552-1]
UniGeneiRn.10791.

3D structure databases

ProteinModelPortaliO35552.
SMRiO35552. Positions 3-446.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO35552. 2 interactions.
STRINGi10116.ENSRNOP00000062965.

PTM databases

PhosphoSiteiO35552.

Proteomic databases

PRIDEiO35552.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000025700; ENSRNOP00000025700; ENSRNOG00000018911. [O35552-3]
ENSRNOT00000025731; ENSRNOP00000025731; ENSRNOG00000018911. [O35552-2]
ENSRNOT00000051067; ENSRNOP00000040928; ENSRNOG00000018911. [O35552-4]
ENSRNOT00000068354; ENSRNOP00000062965; ENSRNOG00000018911. [O35552-1]
GeneIDi117276.
KEGGirno:117276.

Organism-specific databases

CTDi5209.
RGDi619776. Pfkfb3.

Phylogenomic databases

eggNOGiCOG0406.
GeneTreeiENSGT00390000018751.
HOVERGENiHBG005628.
InParanoidiO35552.
KOiK01103.
OMAiADEMPYL.
OrthoDBiEOG7M3J03.
PhylomeDBiO35552.
TreeFamiTF313541.

Enzyme and pathway databases

BRENDAi3.1.3.46. 5301.
ReactomeiREACT_225694. Glycolysis.

Miscellaneous databases

NextBioi620213.
PROiO35552.

Gene expression databases

GenevestigatoriO35552.

Family and domain databases

Gene3Di3.40.50.1240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003094. 6Pfruct_kin.
IPR013079. 6Phosfructo_kin.
IPR016260. Bifunct_6PFK/fruc_bisP_Ptase.
IPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR027417. P-loop_NTPase.
IPR001345. PG/BPGM_mutase_AS.
[Graphical view]
PANTHERiPTHR10606. PTHR10606. 1 hit.
PfamiPF01591. 6PF2K. 1 hit.
PF00300. His_Phos_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000709. 6PFK_2-Ptase. 1 hit.
PRINTSiPR00991. 6PFRUCTKNASE.
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF53254. SSF53254. 1 hit.
PROSITEiPS00175. PG_MUTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Novel isoforms of rat brain fructose 6-phosphate 2-kinase/fructose 2,6-bisphosphatase are generated by tissue-specific alternative splicing."
    Watanabe F., Sakai A., Furuya E.
    J. Neurochem. 69:1-9(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. Sakakibara R.
    Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 61-369.
    Strain: Wistar.
    Tissue: Placenta.

Entry informationi

Entry nameiF263_RAT
AccessioniPrimary (citable) accession number: O35552
Secondary accession number(s): O35096
, O35553, O35554, O35555, O35556, O35557, Q9QWQ5, Q9QWQ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: January 1, 1998
Last modified: January 7, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.