O35547 (ACSL4_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Long-chain-fatty-acid--CoA ligase 4
EC=6.2.1.3

Alternative name(s):
Long-chain acyl-CoA synthetase 4
Short name=LACS 4

Gene names

Name:	Acsl4
Synonyms:	Acs4, Facl4

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	670 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates.
Catalytic activity	ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA.
Cofactor	Magnesium By similarity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	dendritic spine development Inferred from mutant phenotype. Source: RGD fatty acid metabolic process Traceable author statement Ref.1. Source: RGD response to interleukin-15 Inferred from expression pattern. Source: RGD response to nutrient Inferred from expression pattern. Source: RGD
Cellular component	mitochondrial membrane Inferred from direct assay. Source: RGD neuronal cell body Inferred from direct assay. Source: RGD peroxisome Inferred from direct assay. Source: RGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW long-chain fatty acid-CoA ligase activity Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 670	670	Long-chain-fatty-acid--CoA ligase 4		PRO_0000193111
Amino acid modifications
	Modified residue	48	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O35547 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: B7D290973B650CB6	FASTA	670	74,327
	10 20 30 40 50 60 MAKRIKAKPT SDKPGSPYRS VTHFDSLAVI DIPGADTLDK LFDHAVAKFG KKDSLGTREI 70 80 90 100 110 120 LSEENEMQPN GKVFKKLILG NYKWINYLEV NCRVNNFGSG LTALGLKPKN TIAIFCETRA 130 140 150 160 170 180 EWMIAAQTCF KYNFPLVTLY ATLGKEAVVH GLNESEASYL ITSVELLESK LKAALLDINC 190 200 210 220 230 240 VKHIIYVDNK TINRAEYPEG LEIHSMQSVE ELGSKPENSS IPPSRPTPSD MAIVMYTSGS 250 260 270 280 290 300 TGRPKGVMMH HSNLIAGMTG QCERIPGLGP KDTYIGYLPL AHVLELTAEI SCFTYGCRIG 310 320 330 340 350 360 YSSPLTLSDQ SSKIKKGSKG DCTVLKPTLM AAVPEIMDRI YKNVMSKVQE MNYIQKTLFK 370 380 390 400 410 420 IGYDYKLEQI KKGYDAPLCN LILFKKVKAL LGGNVRMMLS GGAPLSPQTH RFMNVCFCCP 430 440 450 460 470 480 IGQGYGLTES CGAGTVTEVT DYTTGRVGAP LICCEIKLKD WQEGGYTVHD KPNPRGEIVI 490 500 510 520 530 540 GGQNISMGYF KNEEKTAEDY SVDENGQRWF CTGDIGEFHP DGCLQIIDRK KDLVKLQAGE 550 560 570 580 590 600 YVSLGKVEAA LKNCPLIDNI CAFAKSDQSY VISFVVPNQK KLTLLAQQKG VEGSWVDICN 610 620 630 640 650 660 NPAMEAEILK EIREAANAMK LERFEIPIKV RLSPEPWTPE TGLVTDAFKL KRKELKNHYL 670 KDIERMYGGK

References

[1]	"A novel arachidonate-preferring acyl-CoA synthetase is present in steroidogenic cells of the rat adrenal, ovary, and testis." Kang M., Fujino T., Sasano H., Minekura H., Yabuki N., Nagura H., Iijima H., Yamamoto T.T. Proc. Natl. Acad. Sci. U.S.A. 94:2880-2884(1997) [PubMed: 9096315] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D85189 mRNA. Translation: BAA22195.1.
IPI	IPI00210503.
RefSeq	NP_446075.1. NM_053623.1.
UniGene	Rn.87821.
3D structure databases
ProteinModelPortal	O35547.
ModBase	Search...
Protein-protein interaction databases
STRING	O35547.
Proteomic databases
PRIDE	O35547.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000026057; ENSRNOP00000026057; ENSRNOG00000019180.
GeneID	113976.
KEGG	rno:113976.
NMPDR	fig|10116.3.peg.31115.
UCSC	NM_053623. rat.
Organism-specific databases
CTD	2182.
RGD	69401. Acsl4.
Phylogenomic databases
eggNOG	roNOG09303.
GeneTree	ENSGT00600000084142.
HOVERGEN	HBG106947.
InParanoid	O35547.
OrthoDB	EOG4SN1N5.
PhylomeDB	O35547.
Enzyme and pathway databases
BRENDA	6.2.1.3. 5301.
Gene expression databases
ArrayExpress	O35547.
Genevestigator	O35547.
GermOnline	ENSRNOG00000019180. Rattus norvegicus.
Family and domain databases
InterPro	IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
KO	K01897.
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	618117.

Entry information

Entry name

ACSL4_RAT

Accession

Primary (citable) accession number: O35547

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program