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Protein

Long-chain-fatty-acid--CoA ligase 4

Gene

Acsl4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates.

Miscellaneous

5 rat isozymes encoded by different genes have been described. ACSL6 corresponds to isozyme 2 (ACS2).1 Publication

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Cofactori

Mg2+By similarity

Kineticsi

  1. KM=34 µM for ATP1 Publication
  2. KM=4.1 µM for CoA1 Publication
  3. KM=5.4 µM for palmitate1 Publication
  4. KM=19.5 µM for oleate1 Publication
  5. KM=10 µM for arachidonate1 Publication
  1. Vmax=2800 nmol/min/mg enzyme with palmitate as substrate1 Publication
  2. Vmax=673 nmol/min/mg enzyme with oleate as substrate1 Publication
  3. Vmax=4200 nmol/min/mg enzyme with arachidonate as substrate1 Publication

GO - Molecular functioni

GO - Biological processi

  • dendritic spine development Source: RGD
  • embryonic process involved in female pregnancy Source: Ensembl
  • fatty acid metabolic process Source: RGD
  • fatty acid transport Source: RGD
  • response to interleukin-15 Source: RGD
  • response to nutrient Source: RGD
  • triglyceride metabolic process Source: RGD

Keywordsi

Molecular functionLigase
Biological processFatty acid metabolism, Lipid metabolism
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.2.1.3. 5301.
ReactomeiR-RNO-75876. Synthesis of very long-chain fatty acyl-CoAs.
SABIO-RKiO35547.

Chemistry databases

SwissLipidsiSLP:000001679.

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain-fatty-acid--CoA ligase 4 (EC:6.2.1.3)
Alternative name(s):
Long-chain acyl-CoA synthetase 4
Short name:
LACS 4
Gene namesi
Name:Acsl4
Synonyms:Acs4, Facl4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi69401. Acsl4.

Subcellular locationi

GO - Cellular componenti

  • ER-mitochondrion membrane contact site Source: Ensembl
  • extracellular exosome Source: Ensembl
  • lipid particle Source: Ensembl
  • mitochondrial membrane Source: RGD
  • neuronal cell body Source: RGD
  • peroxisome Source: RGD

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001931111 – 670Long-chain-fatty-acid--CoA ligase 4Add BLAST670

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei406PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO35547.
PRIDEiO35547.

Expressioni

Gene expression databases

BgeeiENSRNOG00000019180.
GenevisibleiO35547. RN.

Interactioni

Protein-protein interaction databases

MINTiMINT-4567100.
STRINGi10116.ENSRNOP00000026057.

Structurei

3D structure databases

ProteinModelPortaliO35547.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1256. Eukaryota.
COG1022. LUCA.
GeneTreeiENSGT00870000136474.
HOGENOMiHOG000159459.
HOVERGENiHBG106947.
InParanoidiO35547.
KOiK01897.
OMAiAKSDQSY.
OrthoDBiEOG091G02F4.
PhylomeDBiO35547.
TreeFamiTF314012.

Family and domain databases

InterProiView protein in InterPro
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
PfamiView protein in Pfam
PF00501. AMP-binding. 1 hit.
PROSITEiView protein in PROSITE
PS00455. AMP_BINDING. 1 hit.

Sequencei

Sequence statusi: Complete.

O35547-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKRIKAKPT SDKPGSPYRS VTHFDSLAVI DIPGADTLDK LFDHAVAKFG
60 70 80 90 100
KKDSLGTREI LSEENEMQPN GKVFKKLILG NYKWINYLEV NCRVNNFGSG
110 120 130 140 150
LTALGLKPKN TIAIFCETRA EWMIAAQTCF KYNFPLVTLY ATLGKEAVVH
160 170 180 190 200
GLNESEASYL ITSVELLESK LKAALLDINC VKHIIYVDNK TINRAEYPEG
210 220 230 240 250
LEIHSMQSVE ELGSKPENSS IPPSRPTPSD MAIVMYTSGS TGRPKGVMMH
260 270 280 290 300
HSNLIAGMTG QCERIPGLGP KDTYIGYLPL AHVLELTAEI SCFTYGCRIG
310 320 330 340 350
YSSPLTLSDQ SSKIKKGSKG DCTVLKPTLM AAVPEIMDRI YKNVMSKVQE
360 370 380 390 400
MNYIQKTLFK IGYDYKLEQI KKGYDAPLCN LILFKKVKAL LGGNVRMMLS
410 420 430 440 450
GGAPLSPQTH RFMNVCFCCP IGQGYGLTES CGAGTVTEVT DYTTGRVGAP
460 470 480 490 500
LICCEIKLKD WQEGGYTVHD KPNPRGEIVI GGQNISMGYF KNEEKTAEDY
510 520 530 540 550
SVDENGQRWF CTGDIGEFHP DGCLQIIDRK KDLVKLQAGE YVSLGKVEAA
560 570 580 590 600
LKNCPLIDNI CAFAKSDQSY VISFVVPNQK KLTLLAQQKG VEGSWVDICN
610 620 630 640 650
NPAMEAEILK EIREAANAMK LERFEIPIKV RLSPEPWTPE TGLVTDAFKL
660 670
KRKELKNHYL KDIERMYGGK
Length:670
Mass (Da):74,327
Last modified:January 1, 1998 - v1
Checksum:iB7D290973B650CB6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85189 mRNA. Translation: BAA22195.1.
RefSeqiNP_446075.1. NM_053623.1.
XP_006257377.1. XM_006257315.3.
XP_006257378.1. XM_006257316.2.
UniGeneiRn.87821.

Genome annotation databases

EnsembliENSRNOT00000026057; ENSRNOP00000026057; ENSRNOG00000019180.
GeneIDi113976.
KEGGirno:113976.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiACSL4_RAT
AccessioniPrimary (citable) accession number: O35547
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: June 7, 2017
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families