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Protein

Excitatory amino acid transporter 4

Gene

Slc1a6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Transports L-glutamate and also L- and D-aspartate. Seems to act as a symport by cotransporting sodium (By similarity).By similarity

GO - Molecular functioni

  1. L-glutamate transmembrane transporter activity Source: MGI
  2. sodium:dicarboxylate symporter activity Source: InterPro

GO - Biological processi

  1. L-glutamate transmembrane transport Source: GOC
  2. L-glutamate transport Source: MGI
  3. regulation of membrane potential Source: MGI
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Enzyme and pathway databases

ReactomeiREACT_277017. Transport of inorganic cations/anions and amino acids/oligopeptides.
REACT_287745. Glutamate Neurotransmitter Release Cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Excitatory amino acid transporter 4
Alternative name(s):
High-affinity neuronal glutamate transporter
Sodium-dependent glutamate/aspartate transporter
Solute carrier family 1 member 6
Gene namesi
Name:Slc1a6
Synonyms:Eaat4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1096331. Slc1a6.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5252CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei53 – 7321HelicalSequence AnalysisAdd
BLAST
Transmembranei96 – 11621HelicalSequence AnalysisAdd
BLAST
Transmembranei130 – 15021HelicalSequence AnalysisAdd
BLAST
Topological domaini151 – 261111ExtracellularSequence AnalysisAdd
BLAST
Transmembranei262 – 28221HelicalSequence AnalysisAdd
BLAST
Transmembranei303 – 32321HelicalSequence AnalysisAdd
BLAST
Transmembranei342 – 36221HelicalSequence AnalysisAdd
BLAST
Transmembranei367 – 38721HelicalSequence AnalysisAdd
BLAST
Transmembranei415 – 43521HelicalSequence AnalysisAdd
BLAST
Transmembranei457 – 47721HelicalSequence AnalysisAdd
BLAST
Transmembranei478 – 49821HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
  3. membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 561561Excitatory amino acid transporter 4PRO_0000202071Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi213 – 2131N-linked (GlcNAc...)Sequence Analysis
Glycosylationi229 – 2291N-linked (GlcNAc...)Sequence Analysis
Glycosylationi236 – 2361N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiO35544.
PaxDbiO35544.
PRIDEiO35544.

PTM databases

PhosphoSiteiO35544.

Expressioni

Tissue specificityi

Brain specific.

Gene expression databases

BgeeiO35544.
ExpressionAtlasiO35544. baseline and differential.
GenevestigatoriO35544.

Structurei

3D structure databases

ProteinModelPortaliO35544.
SMRiO35544. Positions 62-186, 256-516.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1301.
GeneTreeiENSGT00760000119117.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiO35544.
KOiK05617.
OMAiHVLFTIV.
OrthoDBiEOG7RV9G2.
PhylomeDBiO35544.
TreeFamiTF315206.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35544-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSHGNSLFL RESGAGGGCL QGLQDSLQQR ALRTRLRLQT MTREHVRRFL
60 70 80 90 100
RRNAFILLTV SAVIIGVSLA FALRPYQLSY RQIKYFSFPG ELLMRMLQML
110 120 130 140 150
VLPLIVSSLV TGMASLDNKA TGRMGMRAAV YYMVTTVIAV FIGILMVTII
160 170 180 190 200
HPGKGSKEGL HREGRIETVP TADAFMDLVR NMFPPNLVEA CFKQFKTQYS
210 220 230 240 250
TRVVTRTIVR TDNGSELGAS MSPTSSVENE TSILENVTRA LGTLQEVISF
260 270 280 290 300
EETVPVPGSA NGINALGLVV FSVAFGLVIG GMKHKGRVLR DFFDSLNEAI
310 320 330 340 350
MRLVGIIIWY APVGILFLIA GKILEMEDMA VLGGQLGMYT LTVIVGLFLH
360 370 380 390 400
AGGVLPLIYF LVTHRNPFPF IGGMLQALIT AMGTSSSSAT LPITFRCLEE
410 420 430 440 450
GLGVDRRITR FVLPVGATVN MDGTALYEAL AAIFIAQVNN YELNLGQITT
460 470 480 490 500
ISITATAASV GAAGIPQAGL VTMVIVLTSV GLPTEDITLI IAVDWFLDRL
510 520 530 540 550
RTMTNVLGDS IGAAVIEHLS QRELELQEAE LTLPSLGKPY KSLMAQEKGA
560
SRGRGGNESV M
Length:561
Mass (Da):60,784
Last modified:January 1, 1998 - v1
Checksum:iCCFE3FD499D4CBBB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83262 mRNA. Translation: BAA23640.1.
CCDSiCCDS23970.1.
RefSeqiNP_033226.1. NM_009200.3.
UniGeneiMm.6257.

Genome annotation databases

EnsembliENSMUST00000005490; ENSMUSP00000005490; ENSMUSG00000005357.
GeneIDi20513.
KEGGimmu:20513.
UCSCiuc007fyg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83262 mRNA. Translation: BAA23640.1.
CCDSiCCDS23970.1.
RefSeqiNP_033226.1. NM_009200.3.
UniGeneiMm.6257.

3D structure databases

ProteinModelPortaliO35544.
SMRiO35544. Positions 62-186, 256-516.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiO35544.

Proteomic databases

MaxQBiO35544.
PaxDbiO35544.
PRIDEiO35544.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005490; ENSMUSP00000005490; ENSMUSG00000005357.
GeneIDi20513.
KEGGimmu:20513.
UCSCiuc007fyg.2. mouse.

Organism-specific databases

CTDi6511.
MGIiMGI:1096331. Slc1a6.

Phylogenomic databases

eggNOGiCOG1301.
GeneTreeiENSGT00760000119117.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiO35544.
KOiK05617.
OMAiHVLFTIV.
OrthoDBiEOG7RV9G2.
PhylomeDBiO35544.
TreeFamiTF315206.

Enzyme and pathway databases

ReactomeiREACT_277017. Transport of inorganic cations/anions and amino acids/oligopeptides.
REACT_287745. Glutamate Neurotransmitter Release Cycle.

Miscellaneous databases

NextBioi298715.
PROiO35544.
SOURCEiSearch...

Gene expression databases

BgeeiO35544.
ExpressionAtlasiO35544. baseline and differential.
GenevestigatoriO35544.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Structure and functional expression of the cloned mouse neuronal high-affinity glutamate transporter."
    Maeno-Hikichi Y., Tanaka K., Shibata T., Watanabe M., Inoue Y., Mukainaka Y., Wada K.
    Brain Res. Mol. Brain Res. 48:176-180(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Cerebellum.

Entry informationi

Entry nameiEAA4_MOUSE
AccessioniPrimary (citable) accession number: O35544
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: April 1, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.