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O35544 (EAA4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Excitatory amino acid transporter 4
Alternative name(s):
High-affinity neuronal glutamate transporter
Sodium-dependent glutamate/aspartate transporter
Solute carrier family 1 member 6
Gene names
Name:Slc1a6
Synonyms:Eaat4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length561 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transports L-glutamate and also L- and D-aspartate. Seems to act as a symport by cotransporting sodium By similarity.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Brain specific.

Sequence similarities

Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A6 subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 561561Excitatory amino acid transporter 4
PRO_0000202071

Regions

Topological domain1 – 5252Cytoplasmic Potential
Transmembrane53 – 7321Helical; Potential
Transmembrane96 – 11621Helical; Potential
Transmembrane130 – 15021Helical; Potential
Topological domain151 – 261111Extracellular Potential
Transmembrane262 – 28221Helical; Potential
Transmembrane303 – 32321Helical; Potential
Transmembrane342 – 36221Helical; Potential
Transmembrane367 – 38721Helical; Potential
Transmembrane415 – 43521Helical; Potential
Transmembrane457 – 47721Helical; Potential
Transmembrane478 – 49821Helical; Potential

Amino acid modifications

Glycosylation2131N-linked (GlcNAc...) Potential
Glycosylation2291N-linked (GlcNAc...) Potential
Glycosylation2361N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
O35544 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: CCFE3FD499D4CBBB

FASTA56160,784
        10         20         30         40         50         60 
MSSHGNSLFL RESGAGGGCL QGLQDSLQQR ALRTRLRLQT MTREHVRRFL RRNAFILLTV 

        70         80         90        100        110        120 
SAVIIGVSLA FALRPYQLSY RQIKYFSFPG ELLMRMLQML VLPLIVSSLV TGMASLDNKA 

       130        140        150        160        170        180 
TGRMGMRAAV YYMVTTVIAV FIGILMVTII HPGKGSKEGL HREGRIETVP TADAFMDLVR 

       190        200        210        220        230        240 
NMFPPNLVEA CFKQFKTQYS TRVVTRTIVR TDNGSELGAS MSPTSSVENE TSILENVTRA 

       250        260        270        280        290        300 
LGTLQEVISF EETVPVPGSA NGINALGLVV FSVAFGLVIG GMKHKGRVLR DFFDSLNEAI 

       310        320        330        340        350        360 
MRLVGIIIWY APVGILFLIA GKILEMEDMA VLGGQLGMYT LTVIVGLFLH AGGVLPLIYF 

       370        380        390        400        410        420 
LVTHRNPFPF IGGMLQALIT AMGTSSSSAT LPITFRCLEE GLGVDRRITR FVLPVGATVN 

       430        440        450        460        470        480 
MDGTALYEAL AAIFIAQVNN YELNLGQITT ISITATAASV GAAGIPQAGL VTMVIVLTSV 

       490        500        510        520        530        540 
GLPTEDITLI IAVDWFLDRL RTMTNVLGDS IGAAVIEHLS QRELELQEAE LTLPSLGKPY 

       550        560 
KSLMAQEKGA SRGRGGNESV M 

« Hide

References

[1]"Structure and functional expression of the cloned mouse neuronal high-affinity glutamate transporter."
Maeno-Hikichi Y., Tanaka K., Shibata T., Watanabe M., Inoue Y., Mukainaka Y., Wada K.
Brain Res. Mol. Brain Res. 48:176-180(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Cerebellum.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D83262 mRNA. Translation: BAA23640.1.
CCDSCCDS23970.1.
RefSeqNP_033226.1. NM_009200.3.
UniGeneMm.6257.

3D structure databases

ProteinModelPortalO35544.
SMRO35544. Positions 62-186, 256-516.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteO35544.

Proteomic databases

PaxDbO35544.
PRIDEO35544.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000005490; ENSMUSP00000005490; ENSMUSG00000005357.
GeneID20513.
KEGGmmu:20513.
UCSCuc007fyg.2. mouse.

Organism-specific databases

CTD6511.
MGIMGI:1096331. Slc1a6.

Phylogenomic databases

eggNOGCOG1301.
GeneTreeENSGT00730000110358.
HOGENOMHOG000208776.
HOVERGENHBG000080.
InParanoidO35544.
KOK05617.
OMACPPRPDQ.
OrthoDBEOG7RV9G2.
PhylomeDBO35544.
TreeFamTF315206.

Gene expression databases

BgeeO35544.
GenevestigatorO35544.

Family and domain databases

Gene3D1.10.3860.10. 2 hits.
InterProIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERPTHR11958. PTHR11958. 1 hit.
PfamPF00375. SDF. 1 hit.
[Graphical view]
PRINTSPR00173. EDTRNSPORT.
SUPFAMSSF118215. SSF118215. 2 hits.
PROSITEPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio298715.
PROO35544.
SOURCESearch...

Entry information

Entry nameEAA4_MOUSE
AccessionPrimary (citable) accession number: O35544
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: July 9, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot