Reviewed, UniProtKB/Swiss-Prot O35536 (TFPI2_MOUSE)

Names and origin

Protein names

Recommended name:
    Tissue factor pathway inhibitor 2
      Short name=TFPI-2

Gene names

Name:

Tfpi2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin By similarity.
Subcellular location	Secreted.
Tissue specificity	Highly expressed in placenta. Also expressed in liver and kidney.
Domain	This inhibitor contains three inhibitory domains.
Sequence similarities	Contains 3 BPTI/Kunitz inhibitor domains.

Ontologies

Keywords
Biological process	Blood coagulation
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	blood coagulation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	By similarity
	Chain	23 – 230	208	Tissue factor pathway inhibitor 2		PRO_0000016877
Regions
	Domain	36 – 86	51	BPTI/Kunitz inhibitor 1
	Domain	96 – 146	51	BPTI/Kunitz inhibitor 2
	Domain	156 – 206	51	BPTI/Kunitz inhibitor 3
Sites
	Site	46 – 47	2	Reactive bond By similarity
	Site	106 – 107	2	Reactive bond By similarity
	Site	166 – 167	2	Reactive bond By similarity
Amino acid modifications
	Glycosylation	168	1	N-linked (GlcNAc...) Potential
	Glycosylation	178	1	N-linked (GlcNAc...) Potential
	Disulfide bond	36 ↔ 86		By similarity
	Disulfide bond	45 ↔ 69		By similarity
	Disulfide bond	61 ↔ 82		By similarity
	Disulfide bond	96 ↔ 146		By similarity
	Disulfide bond	105 ↔ 129		By similarity
	Disulfide bond	121 ↔ 142		By similarity
	Disulfide bond	156 ↔ 206		By similarity
	Disulfide bond	165 ↔ 189		By similarity
	Disulfide bond	181 ↔ 202		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O35536-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 57EADB2E36521C7B	FASTA	230	26,137
	10 20 30 40 50 60 MDPAMPLQLW NLPLLLVGSV LGLTSVSAQG NNLEICLLPL DAGPCQALIP KFYYDRDQQK 70 80 90 100 110 120 CRRFNYGGCL GNANNFHSRD LCQQTCGSIE KVPPVCRSEL KTYPCDKPNI RFFFNLNTMT 130 140 150 160 170 180 CEPLRPGLCS RTINVFSEEA TCKGLCEPRK HIPSFCSSPK DEGLCSANVT RFYFNSRNKT 190 200 210 220 230 CETFTYTGCG GNENNFYYLD ACHRACVKGW KKPKRWKIGD FLPRFWKHLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of the cDNA encoding mouse PP5/TFPI-2 and mapping of the gene to chromosome 6." Miyagi Y., Yasumitsu H., Mizushima H., Koshikawa N., Matsuda Y., Itoh H., Hori T., Aoki I., Misugi K., Miyazaki K. DNA Cell Biol. 15:947-954(1996) [PubMed: 8945635] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ICR. Tissue: Placenta.
[2]	"Nucleotide sequence of the gene encoding murine tissue factor pathway inhibitor-2." Kazama Y., Kamei S., Kuijper J.L., Foster D.C., Kisiel W. Thromb. Haemost. 83:141-147(2000) [PubMed: 10669168] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Mammary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D50586 mRNA. Translation: BAA22585.1. AF180353 Genomic DNA. Translation: AAF40412.1. BC021639 mRNA. Translation: AAH21639.1.
IPI	IPI00131643.
RefSeq	NP_033390.1.
UniGene	Mm.25612
3D structure databases
HSSP	HSSP built from PDB template 1IRH based on UniProtKB P10646.
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000029664. Mus musculus. [Contig view]
GeneID	21789.
KEGG	mmu:21789.
Organism-specific databases
MGI	MGI:108543. Tfpi2.
Phylogenomic databases
HOGENOM	O35536.
HOVERGEN	O35536.
OMA	O35536. ACWRIEK.
Gene expression databases
ArrayExpress	O35536.
Bgee	O35536.
CleanEx	MM_TFPI2.
GermOnline	ENSMUSG00000029664. Mus musculus.
Family and domain databases
InterPro	IPR002223. Prot_inh_Kunz-m. IPR008296. Prot_inhib_I2_TFPI. [Graphical view]
Gene3D	G3DSA:4.10.410.10. Prot_inh_Kunz-m. 2 hits.
Pfam	PF00014. Kunitz_BPTI. 3 hits. [Graphical view]
PIRSF	PIRSF001620. TFPI. 1 hit.
ProDom	PD000222. Prot_Inh_Kunz-m. 3 hits. [Graphical view] [Entries sharing at least one domain]
SMART	SM00131. KU. 3 hits. [Graphical view]
PROSITE	PS00280. BPTI_KUNITZ_1. 2 hits. PS50279. BPTI_KUNITZ_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	301148.
SOURCE	Search...

Entry information

Entry name

TFPI2_MOUSE

Accession

Primary (citable) accession number: O35536

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)