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Protein

Methylsterol monooxygenase 1

Gene

Msmo1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the first step in the removal of the two C-4 methyl groups of 4,4-dimethylzymosterol.By similarity

Catalytic activityi

4,4-dimethyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O2 = 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)+ + H2O.By similarity
4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O2 = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)+ + 2 H2O.By similarity
3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)H + O2 = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carboxylate + NAD(P)+ + H2O.By similarity

Cofactori

Fe cationBy similarity

Pathwayi: zymosterol biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes zymosterol from lanosterol.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Lanosterol 14-alpha demethylase (Cyp51a1)
  2. no protein annotated in this organism
  3. Methylsterol monooxygenase 1 (Msmo1)
  4. Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (Nsdhl)
  5. 3-keto-steroid reductase (Hsd17b7)
  6. no protein annotated in this organism
This subpathway is part of the pathway zymosterol biosynthesis, which is itself part of Steroid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes zymosterol from lanosterol, the pathway zymosterol biosynthesis and in Steroid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

Iron, NAD

Enzyme and pathway databases

ReactomeiR-RNO-191273. Cholesterol biosynthesis.
SABIO-RKO35532.
UniPathwayiUPA00770; UER00756.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylsterol monooxygenase 1 (EC:1.14.13.72By similarity)
Alternative name(s):
C-4 methylsterol oxidase
Neuropep 1
RANP-1
Gene namesi
Name:Msmo1
Synonyms:Sc4mol
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 16

Organism-specific databases

RGDi620281. Msmo1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei55 – 75HelicalSequence analysisAdd BLAST21
Transmembranei100 – 120HelicalSequence analysisAdd BLAST21
Transmembranei199 – 219HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001170361 – 293Methylsterol monooxygenase 1Add BLAST293

Proteomic databases

PaxDbiO35532.
PRIDEiO35532.

Expressioni

Gene expression databases

BgeeiENSRNOG00000032297.
GenevisibleiO35532. RN.

Interactioni

Protein-protein interaction databases

MINTiMINT-4575744.
STRINGi10116.ENSRNOP00000043782.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi157 – 161Histidine box-15
Motifi170 – 174Histidine box-25
Motifi249 – 255Histidine box-37

Domaini

The histidine box domains may contain the active site and/or be involved in metal ion binding.

Sequence similaritiesi

Belongs to the sterol desaturase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0873. Eukaryota.
COG3000. LUCA.
GeneTreeiENSGT00530000063017.
HOGENOMiHOG000162289.
HOVERGENiHBG051504.
InParanoidiO35532.
KOiK07750.
OMAiDSQYVAY.
OrthoDBiEOG091G0BM3.
PhylomeDBiO35532.
TreeFamiTF354294.

Family and domain databases

InterProiIPR006694. Fatty_acid_hydroxylase.
[Graphical view]
PfamiPF04116. FA_hydroxylase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35532-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMNKSVGLF SSASLAVDYV DSLLPENPLQ EPFKNAWVYM LDNYTKFQIA
60 70 80 90 100
TWGSLIVHET IYFLFSLPGF LFQFIPFMRK YKIQKDKPET FEGQWKCLKG
110 120 130 140 150
ILFNHFFIQL PLICGTYYFT EFFNIPYDWE RMPRWYFTLA RCLGCAVIED
160 170 180 190 200
TWHYFLHRLL HHKRIYKYIH KVHHEFQAPF GIEAEYAHPL ETLILGTGFF
210 220 230 240 250
IGIVLLCDHV ILLWAWVTMR LLETIDVHSG YDIPLNPLNY IPFYTGARHH
260 270 280 290
DFHHMNFIGN YASTFTWWDR IFGTDVQYHA YTEKMKKLGK KSE
Length:293
Mass (Da):34,964
Last modified:January 1, 1998 - v1
Checksum:iFEE8C22FDC5F38D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50559 mRNA. Translation: BAA23329.1.
BC063155 mRNA. Translation: AAH63155.1.
RefSeqiNP_543162.1. NM_080886.1.
UniGeneiRn.7167.

Genome annotation databases

EnsembliENSRNOT00000044171; ENSRNOP00000043782; ENSRNOG00000032297.
GeneIDi140910.
KEGGirno:140910.
UCSCiRGD:620281. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50559 mRNA. Translation: BAA23329.1.
BC063155 mRNA. Translation: AAH63155.1.
RefSeqiNP_543162.1. NM_080886.1.
UniGeneiRn.7167.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4575744.
STRINGi10116.ENSRNOP00000043782.

Proteomic databases

PaxDbiO35532.
PRIDEiO35532.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000044171; ENSRNOP00000043782; ENSRNOG00000032297.
GeneIDi140910.
KEGGirno:140910.
UCSCiRGD:620281. rat.

Organism-specific databases

CTDi6307.
RGDi620281. Msmo1.

Phylogenomic databases

eggNOGiKOG0873. Eukaryota.
COG3000. LUCA.
GeneTreeiENSGT00530000063017.
HOGENOMiHOG000162289.
HOVERGENiHBG051504.
InParanoidiO35532.
KOiK07750.
OMAiDSQYVAY.
OrthoDBiEOG091G0BM3.
PhylomeDBiO35532.
TreeFamiTF354294.

Enzyme and pathway databases

UniPathwayiUPA00770; UER00756.
ReactomeiR-RNO-191273. Cholesterol biosynthesis.
SABIO-RKO35532.

Miscellaneous databases

PROiO35532.

Gene expression databases

BgeeiENSRNOG00000032297.
GenevisibleiO35532. RN.

Family and domain databases

InterProiIPR006694. Fatty_acid_hydroxylase.
[Graphical view]
PfamiPF04116. FA_hydroxylase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMSMO1_RAT
AccessioniPrimary (citable) accession number: O35532
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2003
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.