Reviewed, UniProtKB/Swiss-Prot O35532 (ERG25_RAT)

Names and origin

Protein names

Recommended name:
    C-4 methylsterol oxidase
    EC=1.14.13.72
Alternative name(s):
    Methylsterol monooxygenase
    Neuropep 1
    RANP-1

Gene names

Name:

Sc4mol

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	4,4-dimethyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O2 = 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)+ + H2O. 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O2 = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)+ + 2 H2O. 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)H + O2 = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carboxylate + NAD(P)+ + H2O.
Cofactor	Iron Probable.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 3/6.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Probable.
Domain	The histidine box domains may contain the active site and/or be involved in metal ion binding.
Sequence similarities	Belongs to the sterol desaturase family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	Iron NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	C-4 methylsterol oxidase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 293	293	C-4 methylsterol oxidase		PRO_0000117036
Regions
	Transmembrane	55 – 75	21	Potential
	Transmembrane	100 – 120	21	Potential
	Transmembrane	199 – 219	21	Potential
	Motif	157 – 161	5	Histidine box-1
	Motif	170 – 174	5	Histidine box-2
	Motif	249 – 255	7	Histidine box-3

Sequences

	Sequence		Length	Mass (Da)
	O35532-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: FEE8C22FDC5F38D7	FASTA	293	34,964
	10 20 30 40 50 60 MAMNKSVGLF SSASLAVDYV DSLLPENPLQ EPFKNAWVYM LDNYTKFQIA TWGSLIVHET 70 80 90 100 110 120 IYFLFSLPGF LFQFIPFMRK YKIQKDKPET FEGQWKCLKG ILFNHFFIQL PLICGTYYFT 130 140 150 160 170 180 EFFNIPYDWE RMPRWYFTLA RCLGCAVIED TWHYFLHRLL HHKRIYKYIH KVHHEFQAPF 190 200 210 220 230 240 GIEAEYAHPL ETLILGTGFF IGIVLLCDHV ILLWAWVTMR LLETIDVHSG YDIPLNPLNY 250 260 270 280 290 IPFYTGARHH DFHHMNFIGN YASTFTWWDR IFGTDVQYHA YTEKMKKLGK KSE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a novel gene (neurorep 1) expressed in nerve cells and up-regulated after axotomy." Uwabe K., Gahara Y., Yamada H., Miyake T., Kitamura T. Neuroscience 80:501-509(1997) [PubMed: 9284352] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D50559 mRNA. Translation: BAA23329.1. BC063155 mRNA. Translation: AAH63155.1.
IPI	IPI00210465.
RefSeq	NP_543162.1.
UniGene	Rn.7167
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	O35532.
Genome annotation databases
Ensembl	ENSRNOG00000032297. Rattus norvegicus. [Contig view]
GeneID	140910.
KEGG	rno:140910.
Organism-specific databases
RGD	620281. Sc4mol.
Phylogenomic databases
HOVERGEN	O35532.
OMA	O35532. NPLHLIP.
Enzyme and pathway databases
BRENDA	1.14.13.72. 248.
Gene expression databases
ArrayExpress	O35532.
GermOnline	ENSRNOG00000032297. Rattus norvegicus.
Family and domain databases
InterPro	IPR006694. Fatty_acid_hydroxylase. [Graphical view]
Pfam	PF04116. FA_hydroxylase. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	620749.

Entry information

Entry name

ERG25_RAT

Accession

Primary (citable) accession number: O35532

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 22, 2003
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)