Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O35526

- STX1A_MOUSE

UniProt

O35526 - STX1A_MOUSE

Protein

Syntaxin-1A

Gene

Stx1a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 3 (19 Dec 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca2+-regulation of exocytosis acrosomal reaction in sperm.1 Publication

    GO - Molecular functioni

    1. calcium channel inhibitor activity Source: MGI
    2. glycoprotein binding Source: MGI
    3. protein binding Source: UniProtKB
    4. SNARE binding Source: MGI

    GO - Biological processi

    1. calcium ion-dependent exocytosis Source: MGI
    2. intracellular protein transport Source: InterPro
    3. positive regulation of exocytosis Source: Ensembl
    4. positive regulation of neurotransmitter secretion Source: Ensembl
    5. response to gravity Source: Ensembl
    6. synaptic vesicle docking involved in exocytosis Source: Ensembl

    Keywords - Biological processi

    Exocytosis, Neurotransmitter transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_205038. Acetylcholine Neurotransmitter Release Cycle.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Syntaxin-1A
    Alternative name(s):
    Neuron-specific antigen HPC-1
    Gene namesi
    Name:Stx1a
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:109355. Stx1a.

    Subcellular locationi

    Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane 1 Publication; Single-pass type IV membrane protein 1 Publication. Cell junctionsynapsesynaptosome 1 Publication

    GO - Cellular componenti

    1. actomyosin Source: Ensembl
    2. cell junction Source: UniProtKB-KW
    3. integral component of membrane Source: UniProtKB-KW
    4. membrane Source: MGI
    5. neuron projection Source: MGI
    6. plasma membrane Source: MGI
    7. secretory granule Source: Ensembl
    8. SNARE complex Source: UniProtKB
    9. synaptic vesicle Source: UniProtKB
    10. synaptic vesicle membrane Source: MGI
    11. synaptobrevin 2-SNAP-25-syntaxin-1a complex Source: MGI
    12. synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex Source: MGI
    13. synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex Source: Ensembl

    Keywords - Cellular componenti

    Cell junction, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 288288Syntaxin-1APRO_0000210187Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei14 – 141Phosphoserine1 Publication
    Modified residuei188 – 1881Phosphoserine; by DAPK1By similarity

    Post-translational modificationi

    Phosphorylated by CK2. Phosphorylation at Ser-188 by DAPK1 significantly decreases its interaction with STXBP1 By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiO35526.
    PaxDbiO35526.
    PRIDEiO35526.

    PTM databases

    PhosphoSiteiO35526.

    Expressioni

    Gene expression databases

    ArrayExpressiO35526.
    BgeeiO35526.
    CleanExiMM_STX1A.
    GenevestigatoriO35526.

    Interactioni

    Subunit structurei

    Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Interacts with VAPA and SYBU. Binds STXBP6. Found in a complex with VAMP8 and SNAP23. Found in a ternary complex with STX1A and SNAP25 By similarity. Interacts with SLC6A4 By similarity. Binds SYTL4. Interacts with OTOF and LGI3. Interacts with SYT6 and SYT8; the interaction is Ca2+-dependent. Interacts with STXBP1 and DAPK1 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Syt2P460972EBI-400878,EBI-457969

    Protein-protein interaction databases

    BioGridi203561. 15 interactions.
    DIPiDIP-31950N.
    IntActiO35526. 17 interactions.
    MINTiMINT-2411610.

    Structurei

    3D structure databases

    ProteinModelPortaliO35526.
    SMRiO35526. Positions 27-286.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 265265CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini287 – 2882ExtracellularSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei266 – 28621Helical; Anchor for type IV membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini192 – 25463t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili68 – 10942Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the syntaxin family.Curated
    Contains 1 t-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5074.
    HOGENOMiHOG000286023.
    HOVERGENiHBG000497.
    InParanoidiO35526.
    KOiK04560.
    OMAiHEETKAV.
    OrthoDBiEOG7X9G7R.
    PhylomeDBiO35526.
    TreeFamiTF313763.

    Family and domain databases

    InterProiIPR028669. STX1A/1B.
    IPR006012. Syntaxin/epimorphin_CS.
    IPR006011. Syntaxin_N.
    IPR010989. t-SNARE.
    IPR000727. T_SNARE_dom.
    [Graphical view]
    PANTHERiPTHR19957:SF84. PTHR19957:SF84. 1 hit.
    PfamiPF05739. SNARE. 1 hit.
    PF00804. Syntaxin. 1 hit.
    [Graphical view]
    SMARTiSM00503. SynN. 1 hit.
    SM00397. t_SNARE. 1 hit.
    [Graphical view]
    SUPFAMiSSF47661. SSF47661. 1 hit.
    PROSITEiPS00914. SYNTAXIN. 1 hit.
    PS50192. T_SNARE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O35526-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKDRTQELRT AKDSDDDDDV TVTVDRDRFM DEFFEQVEEI RGFIDKIAEN    50
    VEEVKRKHSA ILASPNPDEK TKEELEELMS DIKKTANKVR SKLKSIEQSI 100
    EQEEGLNRSS ADLRIRKTQH STLSRKFVEV MSEYNATQSD YRERCKGRIQ 150
    RQLEITGRTT TSEELEDMLE SGNPAIFASG IIMDSSISKQ ALSEIETRHS 200
    EIIKLETSIR ELHDMFMDMA MLVESQGEMI DRIEYNVEHA VDYVERAVSD 250
    TKKAVKYQSK ARRKKIMIII CCVILGIIIA STIGGIFG 288
    Length:288
    Mass (Da):33,054
    Last modified:December 19, 2001 - v3
    Checksum:i8F8255F2EEF886CA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D45208 mRNA. Translation: BAA28865.2.
    CCDSiCCDS19731.1.
    RefSeqiNP_058081.2. NM_016801.3.
    UniGeneiMm.6225.

    Genome annotation databases

    EnsembliENSMUST00000005509; ENSMUSP00000005509; ENSMUSG00000007207.
    GeneIDi20907.
    KEGGimmu:20907.
    UCSCiuc008zxk.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D45208 mRNA. Translation: BAA28865.2 .
    CCDSi CCDS19731.1.
    RefSeqi NP_058081.2. NM_016801.3.
    UniGenei Mm.6225.

    3D structure databases

    ProteinModelPortali O35526.
    SMRi O35526. Positions 27-286.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203561. 15 interactions.
    DIPi DIP-31950N.
    IntActi O35526. 17 interactions.
    MINTi MINT-2411610.

    PTM databases

    PhosphoSitei O35526.

    Proteomic databases

    MaxQBi O35526.
    PaxDbi O35526.
    PRIDEi O35526.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000005509 ; ENSMUSP00000005509 ; ENSMUSG00000007207 .
    GeneIDi 20907.
    KEGGi mmu:20907.
    UCSCi uc008zxk.1. mouse.

    Organism-specific databases

    CTDi 6804.
    MGIi MGI:109355. Stx1a.

    Phylogenomic databases

    eggNOGi COG5074.
    HOGENOMi HOG000286023.
    HOVERGENi HBG000497.
    InParanoidi O35526.
    KOi K04560.
    OMAi HEETKAV.
    OrthoDBi EOG7X9G7R.
    PhylomeDBi O35526.
    TreeFami TF313763.

    Enzyme and pathway databases

    Reactomei REACT_205038. Acetylcholine Neurotransmitter Release Cycle.

    Miscellaneous databases

    NextBioi 299781.
    PROi O35526.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O35526.
    Bgeei O35526.
    CleanExi MM_STX1A.
    Genevestigatori O35526.

    Family and domain databases

    InterProi IPR028669. STX1A/1B.
    IPR006012. Syntaxin/epimorphin_CS.
    IPR006011. Syntaxin_N.
    IPR010989. t-SNARE.
    IPR000727. T_SNARE_dom.
    [Graphical view ]
    PANTHERi PTHR19957:SF84. PTHR19957:SF84. 1 hit.
    Pfami PF05739. SNARE. 1 hit.
    PF00804. Syntaxin. 1 hit.
    [Graphical view ]
    SMARTi SM00503. SynN. 1 hit.
    SM00397. t_SNARE. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47661. SSF47661. 1 hit.
    PROSITEi PS00914. SYNTAXIN. 1 hit.
    PS50192. T_SNARE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Fujiwara T., Genda M., Nagai A., Okazaki M., Watanabe T., Nagamatsu S., Akagawa K.
      Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. Lubec G., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 29-41; 47-56; 58-84; 95-108; 126-142; 152-158 AND 233-246, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain.
    3. "Granuphilin modulates the exocytosis of secretory granules through interaction with syntaxin 1a."
      Torii S., Zhao S., Yi Z., Takeuchi T., Izumi T.
      Mol. Cell. Biol. 22:5518-5526(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SYTL4.
    4. "Synaptotagmin VI and VIII and syntaxin 2 are essential for the mouse sperm acrosome reaction."
      Hutt D.M., Baltz J.M., Ngsee J.K.
      J. Biol. Chem. 280:20197-20203(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SYT6 AND SYT8.
    5. "Otoferlin, defective in a human deafness form, is essential for exocytosis at the auditory ribbon synapse."
      Roux I., Safieddine S., Nouvian R., Grati M., Simmler M.-C., Bahloul A., Perfettini I., Le Gall M., Rostaing P., Hamard G., Triller A., Avan P., Moser T., Petit C.
      Cell 127:277-289(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH OTOF.
      Strain: BALB/c.
      Tissue: Cochlea.
    6. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    7. "Leucine-rich glioma inactivated 3 associates with syntaxin 1."
      Park W.-J., Lee S.E., Kwon N.S., Baek K.J., Kim D.-S., Yun H.-Y.
      Neurosci. Lett. 444:240-244(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH LGI3.

    Entry informationi

    Entry nameiSTX1A_MOUSE
    AccessioniPrimary (citable) accession number: O35526
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: December 19, 2001
    Last modified: October 1, 2014
    This is version 129 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3