O35526 (STX1A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Syntaxin-1A

Alternative name(s):
Neuron-specific antigen HPC-1

Gene names

Name:

Stx1a

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca2+-regulation of exocytosis acrosomal reaction in sperm. Ref.4
Subunit structure	Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Interacts with VAPA and SYBU. Binds STXBP6. Found in a complex with VAMP8 and SNAP23. Found in a ternary complex with STX1A and SNAP25 By similarity. Interacts with SLC6A4 By similarity. Binds SYTL4. Interacts with OTOF and LGI3. Interacts with SYT6 and SYT8; the interaction is Ca2+-dependent. Interacts with STXBP1 and DAPK1 By similarity. Ref.3 Ref.4 Ref.5 Ref.8
Subcellular location	Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Single-pass type IV membrane protein. Cell junction › synapse › synaptosome Ref.8.
Post-translational modification	Phosphorylated by CK2. Phosphorylation at Ser-188 by DAPK1 significantly decreases its interaction with STXBP1 By similarity.
Sequence similarities	Belongs to the syntaxin family. Contains 1 t-SNARE coiled-coil homology domain.

Ontologies

Keywords
Biological process	Exocytosis Neurotransmitter transport Transport
Cellular component	Cell junction Cytoplasmic vesicle Membrane Synapse Synaptosome
Domain	Coiled coil Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	calcium ion-dependent exocytosis Inferred from mutant phenotype PubMed 10468580. Source: MGI intracellular protein transport Inferred from electronic annotation. Source: InterPro positive regulation of exocytosis Inferred from electronic annotation. Source: Compara positive regulation of neurotransmitter secretion Inferred from electronic annotation. Source: Compara response to gravity Inferred from electronic annotation. Source: Compara synaptic vesicle docking involved in exocytosis Inferred from electronic annotation. Source: Compara
Cellular_component	actomyosin Inferred from electronic annotation. Source: Compara cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW neuron projection Inferred from direct assay PubMed 15935055. Source: MGI plasma membrane Inferred from direct assay PubMed 10468580. Source: MGI secretory granule Inferred from electronic annotation. Source: Compara synaptic vesicle membrane Inferred from direct assay PubMed 12805290. Source: MGI synaptobrevin 2-SNAP-25-syntaxin-1a complex Inferred from direct assay PubMed 15935055PubMed 21040848. Source: MGI synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex Inferred from sequence orthology PubMed 7553862. Source: MGI
Molecular_function	SNARE binding Inferred from direct assay PubMed 12145198. Source: MGI calcium channel inhibitor activity Inferred from mutant phenotype PubMed 10468580. Source: MGI glycoprotein binding Inferred from sequence orthology PubMed 7553862. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	Syntaxin-1A		PRO_0000210187
Regions
	Topological domain	1 – 265	265	Cytoplasmic Potential
	Transmembrane	266 – 286	21	Helical; Anchor for type IV membrane protein; Potential
	Topological domain	287 – 288	2	Extracellular Potential
	Domain	192 – 254	63	t-SNARE coiled-coil homology
	Coiled coil	68 – 109	42	Potential
Amino acid modifications
	Modified residue	14	1	Phosphoserine Ref.6 Ref.7
	Modified residue	188	1	Phosphoserine; by DAPK1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	O35526 [UniParc]. Last modified December 19, 2001. Version 3. Checksum: 8F8255F2EEF886CA	FASTA	288	33,054
	10 20 30 40 50 60 MKDRTQELRT AKDSDDDDDV TVTVDRDRFM DEFFEQVEEI RGFIDKIAEN VEEVKRKHSA 70 80 90 100 110 120 ILASPNPDEK TKEELEELMS DIKKTANKVR SKLKSIEQSI EQEEGLNRSS ADLRIRKTQH 130 140 150 160 170 180 STLSRKFVEV MSEYNATQSD YRERCKGRIQ RQLEITGRTT TSEELEDMLE SGNPAIFASG 190 200 210 220 230 240 IIMDSSISKQ ALSEIETRHS EIIKLETSIR ELHDMFMDMA MLVESQGEMI DRIEYNVEHA 250 260 270 280 VDYVERAVSD TKKAVKYQSK ARRKKIMIII CCVILGIIIA STIGGIFG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Fujiwara T., Genda M., Nagai A., Okazaki M., Watanabe T., Nagamatsu S., Akagawa K. Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 29-41; 47-56; 58-84; 95-108; 126-142; 152-158 AND 233-246, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[3]	"Granuphilin modulates the exocytosis of secretory granules through interaction with syntaxin 1a." Torii S., Zhao S., Yi Z., Takeuchi T., Izumi T. Mol. Cell. Biol. 22:5518-5526(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SYTL4.
[4]	"Synaptotagmin VI and VIII and syntaxin 2 are essential for the mouse sperm acrosome reaction." Hutt D.M., Baltz J.M., Ngsee J.K. J. Biol. Chem. 280:20197-20203(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SYT6 AND SYT8.
[5]	"Otoferlin, defective in a human deafness form, is essential for exocytosis at the auditory ribbon synapse." Roux I., Safieddine S., Nouvian R., Grati M., Simmler M.-C., Bahloul A., Perfettini I., Le Gall M., Rostaing P., Hamard G., Triller A., Avan P., Moser T., Petit C. Cell 127:277-289(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH OTOF. Strain: BALB/c. Tissue: Cochlea.
[6]	"Comprehensive identification of phosphorylation sites in postsynaptic density preparations." Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L. Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14, MASS SPECTROMETRY. Tissue: Brain.
[7]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14, MASS SPECTROMETRY. Tissue: Brain cortex.
[8]	"Leucine-rich glioma inactivated 3 associates with syntaxin 1." Park W.-J., Lee S.E., Kwon N.S., Baek K.J., Kim D.-S., Yun H.-Y. Neurosci. Lett. 444:240-244(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH LGI3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D45208 mRNA. Translation: BAA28865.2.
IPI	IPI00131618.
RefSeq	NP_058081.2. NM_016801.3.
UniGene	Mm.6225.
3D structure databases
ProteinModelPortal	O35526.
SMR	O35526. Positions 2-286.
ModBase	Search...
Protein-protein interaction databases
IntAct	O35526. 9 interactions.
MINT	MINT-2411610.
PTM databases
PhosphoSite	O35526.
Proteomic databases
PaxDb	O35526.
PRIDE	O35526.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000005509; ENSMUSP00000005509; ENSMUSG00000007207.
GeneID	20907.
KEGG	mmu:20907.
Organism-specific databases
CTD	6804.
MGI	MGI:109355. Stx1a.
Phylogenomic databases
eggNOG	COG5074.
HOGENOM	HOG000286023.
HOVERGEN	HBG000497.
InParanoid	O35526.
KO	K04560.
OMA	HVINASH.
OrthoDB	EOG4PC9SV.
Enzyme and pathway databases
Reactome	REACT_112621. Metabolism.
Gene expression databases
ArrayExpress	O35526.
Bgee	O35526.
CleanEx	MM_STX1A.
Genevestigator	O35526.
GermOnline	ENSMUSG00000007207. Mus musculus.
Family and domain databases
InterPro	IPR015709. Syntaxin-1. IPR006012. Syntaxin/epimorphin_CS. IPR006011. Syntaxin_N. IPR010989. t-SNARE. IPR000727. T_SNARE_dom. [Graphical view]
PANTHER	PTHR19957:SF35. PTHR19957:SF35. 1 hit.
Pfam	PF05739. SNARE. 1 hit. PF00804. Syntaxin. 1 hit. [Graphical view]
SMART	SM00503. SynN. 1 hit. SM00397. t_SNARE. 1 hit. [Graphical view]
SUPFAM	SSF47661. t-snare. 1 hit.
PROSITE	PS00914. SYNTAXIN. 1 hit. PS50192. T_SNARE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	299781.
SOURCE	Search...

Entry information

Entry name

STX1A_MOUSE

Accession

Primary (citable) accession number: O35526

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	December 19, 2001
Last modified:	April 3, 2013
This is version 116 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program