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O35526 (STX1A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Syntaxin-1A
Alternative name(s):
Neuron-specific antigen HPC-1
Gene names
Name:Stx1a
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length288 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca2+-regulation of exocytosis acrosomal reaction in sperm. Ref.4

Subunit structure

Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Interacts with VAPA and SYBU. Binds STXBP6. Found in a complex with VAMP8 and SNAP23. Found in a ternary complex with STX1A and SNAP25 By similarity. Interacts with SLC6A4 By similarity. Binds SYTL4. Interacts with OTOF and LGI3. Interacts with SYT6 and SYT8; the interaction is Ca2+-dependent. Interacts with STXBP1 and DAPK1 By similarity. Ref.3 Ref.4 Ref.5 Ref.7

Subcellular location

Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane; Single-pass type IV membrane protein. Cell junctionsynapsesynaptosome Ref.7.

Post-translational modification

Phosphorylated by CK2. Phosphorylation at Ser-188 by DAPK1 significantly decreases its interaction with STXBP1 By similarity.

Sequence similarities

Belongs to the syntaxin family.

Contains 1 t-SNARE coiled-coil homology domain.

Ontologies

Keywords
   Biological processExocytosis
Neurotransmitter transport
Transport
   Cellular componentCell junction
Cytoplasmic vesicle
Membrane
Synapse
Synaptosome
   DomainCoiled coil
Transmembrane
Transmembrane helix
   PTMPhosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcalcium ion-dependent exocytosis

Inferred from mutant phenotype PubMed 10468580. Source: MGI

intracellular protein transport

Inferred from electronic annotation. Source: InterPro

positive regulation of exocytosis

Inferred from electronic annotation. Source: Ensembl

positive regulation of neurotransmitter secretion

Inferred from electronic annotation. Source: Ensembl

response to gravity

Inferred from electronic annotation. Source: Ensembl

synaptic vesicle docking involved in exocytosis

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentSNARE complex

Inferred from sequence or structural similarity. Source: UniProtKB

actomyosin

Inferred from electronic annotation. Source: Ensembl

cell junction

Inferred from electronic annotation. Source: UniProtKB-KW

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

membrane

Inferred from direct assay PubMed 10712642. Source: MGI

neuron projection

Inferred from direct assay PubMed 15935055. Source: MGI

plasma membrane

Inferred from direct assay PubMed 10468580. Source: MGI

secretory granule

Inferred from electronic annotation. Source: Ensembl

synaptic vesicle

Inferred from direct assay Ref.7. Source: UniProtKB

synaptic vesicle membrane

Inferred from direct assay PubMed 12805290. Source: MGI

synaptobrevin 2-SNAP-25-syntaxin-1a complex

Inferred from direct assay PubMed 15935055PubMed 21040848. Source: MGI

synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex

Inferred from sequence orthology PubMed 7553862. Source: MGI

synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionSNARE binding

Inferred from direct assay PubMed 12145198. Source: MGI

calcium channel inhibitor activity

Inferred from mutant phenotype PubMed 10468580. Source: MGI

glycoprotein binding

Inferred from sequence orthology PubMed 7553862. Source: MGI

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Syt2P460972EBI-400878,EBI-457969

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 288288Syntaxin-1A
PRO_0000210187

Regions

Topological domain1 – 265265Cytoplasmic Potential
Transmembrane266 – 28621Helical; Anchor for type IV membrane protein; Potential
Topological domain287 – 2882Extracellular Potential
Domain192 – 25463t-SNARE coiled-coil homology
Coiled coil68 – 10942 Potential

Amino acid modifications

Modified residue141Phosphoserine Ref.6
Modified residue1881Phosphoserine; by DAPK1 By similarity

Sequences

Sequence LengthMass (Da)Tools
O35526 [UniParc].

Last modified December 19, 2001. Version 3.
Checksum: 8F8255F2EEF886CA

FASTA28833,054
        10         20         30         40         50         60 
MKDRTQELRT AKDSDDDDDV TVTVDRDRFM DEFFEQVEEI RGFIDKIAEN VEEVKRKHSA 

        70         80         90        100        110        120 
ILASPNPDEK TKEELEELMS DIKKTANKVR SKLKSIEQSI EQEEGLNRSS ADLRIRKTQH 

       130        140        150        160        170        180 
STLSRKFVEV MSEYNATQSD YRERCKGRIQ RQLEITGRTT TSEELEDMLE SGNPAIFASG 

       190        200        210        220        230        240 
IIMDSSISKQ ALSEIETRHS EIIKLETSIR ELHDMFMDMA MLVESQGEMI DRIEYNVEHA 

       250        260        270        280 
VDYVERAVSD TKKAVKYQSK ARRKKIMIII CCVILGIIIA STIGGIFG 

« Hide

References

« Hide 'large scale' references
[1]Fujiwara T., Genda M., Nagai A., Okazaki M., Watanabe T., Nagamatsu S., Akagawa K.
Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]Lubec G., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 29-41; 47-56; 58-84; 95-108; 126-142; 152-158 AND 233-246, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain.
[3]"Granuphilin modulates the exocytosis of secretory granules through interaction with syntaxin 1a."
Torii S., Zhao S., Yi Z., Takeuchi T., Izumi T.
Mol. Cell. Biol. 22:5518-5526(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SYTL4.
[4]"Synaptotagmin VI and VIII and syntaxin 2 are essential for the mouse sperm acrosome reaction."
Hutt D.M., Baltz J.M., Ngsee J.K.
J. Biol. Chem. 280:20197-20203(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SYT6 AND SYT8.
[5]"Otoferlin, defective in a human deafness form, is essential for exocytosis at the auditory ribbon synapse."
Roux I., Safieddine S., Nouvian R., Grati M., Simmler M.-C., Bahloul A., Perfettini I., Le Gall M., Rostaing P., Hamard G., Triller A., Avan P., Moser T., Petit C.
Cell 127:277-289(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH OTOF.
Strain: BALB/c.
Tissue: Cochlea.
[6]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
[7]"Leucine-rich glioma inactivated 3 associates with syntaxin 1."
Park W.-J., Lee S.E., Kwon N.S., Baek K.J., Kim D.-S., Yun H.-Y.
Neurosci. Lett. 444:240-244(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH LGI3.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D45208 mRNA. Translation: BAA28865.2.
RefSeqNP_058081.2. NM_016801.3.
UniGeneMm.6225.

3D structure databases

ProteinModelPortalO35526.
SMRO35526. Positions 27-286.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203561. 15 interactions.
IntActO35526. 16 interactions.
MINTMINT-2411610.

PTM databases

PhosphoSiteO35526.

Proteomic databases

PaxDbO35526.
PRIDEO35526.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000005509; ENSMUSP00000005509; ENSMUSG00000007207.
GeneID20907.
KEGGmmu:20907.
UCSCuc008zxk.1. mouse.

Organism-specific databases

CTD6804.
MGIMGI:109355. Stx1a.

Phylogenomic databases

eggNOGCOG5074.
HOGENOMHOG000286023.
HOVERGENHBG000497.
InParanoidO35526.
KOK04560.
OMAWICFWIV.
OrthoDBEOG7X9G7R.
PhylomeDBO35526.
TreeFamTF313763.

Enzyme and pathway databases

ReactomeREACT_188937. Metabolism.

Gene expression databases

ArrayExpressO35526.
BgeeO35526.
CleanExMM_STX1A.
GenevestigatorO35526.

Family and domain databases

InterProIPR028669. STX1A/1B.
IPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PANTHERPTHR19957:SF33. PTHR19957:SF33. 1 hit.
PfamPF05739. SNARE. 1 hit.
PF00804. Syntaxin. 1 hit.
[Graphical view]
SMARTSM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMSSF47661. SSF47661. 1 hit.
PROSITEPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio299781.
PROO35526.
SOURCESearch...

Entry information

Entry nameSTX1A_MOUSE
AccessionPrimary (citable) accession number: O35526
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 19, 2001
Last modified: April 16, 2014
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot