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Protein

Syntaxin-1A

Gene

Stx1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca2+-regulation of exocytosis acrosomal reaction in sperm.1 Publication

GO - Molecular functioni

  1. calcium channel inhibitor activity Source: MGI
  2. chloride channel inhibitor activity Source: MGI
  3. glycoprotein binding Source: MGI
  4. kinase binding Source: MGI
  5. myosin binding Source: GO_Central
  6. SNAP receptor activity Source: GO_Central
  7. SNARE binding Source: MGI

GO - Biological processi

  1. calcium ion-dependent exocytosis Source: MGI
  2. intracellular protein transport Source: GO_Central
  3. positive regulation of excitatory postsynaptic membrane potential Source: ParkinsonsUK-UCL
  4. positive regulation of exocytosis Source: Ensembl
  5. positive regulation of neurotransmitter secretion Source: ParkinsonsUK-UCL
  6. regulation of synaptic vesicle priming Source: ParkinsonsUK-UCL
  7. response to gravity Source: Ensembl
  8. secretion by cell Source: MGI
  9. synaptic vesicle docking involved in exocytosis Source: Ensembl
  10. synaptic vesicle fusion to presynaptic membrane Source: GO_Central
  11. vesicle docking Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Exocytosis, Neurotransmitter transport, Transport

Enzyme and pathway databases

ReactomeiREACT_205038. Acetylcholine Neurotransmitter Release Cycle.
REACT_229514. Glutamate Neurotransmitter Release Cycle.
REACT_242222. Norepinephrine Neurotransmitter Release Cycle.
REACT_245631. GABA synthesis, release, reuptake and degradation.
REACT_263148. Regulation of insulin secretion.

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin-1A
Alternative name(s):
Neuron-specific antigen HPC-1
Gene namesi
Name:Stx1a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:109355. Stx1a.

Subcellular locationi

Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane 1 Publication; Single-pass type IV membrane protein 1 Publication. Cell junctionsynapsesynaptosome 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 265265CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei266 – 28621Helical; Anchor for type IV membrane proteinSequence AnalysisAdd
BLAST
Topological domaini287 – 2882ExtracellularSequence Analysis

GO - Cellular componenti

  1. actomyosin Source: Ensembl
  2. cell junction Source: UniProtKB-KW
  3. endomembrane system Source: GO_Central
  4. integral component of membrane Source: GO_Central
  5. membrane Source: MGI
  6. neuron projection Source: MGI
  7. plasma membrane Source: MGI
  8. secretory granule Source: Ensembl
  9. SNARE complex Source: UniProtKB
  10. synaptic vesicle Source: UniProtKB
  11. synaptic vesicle membrane Source: MGI
  12. synaptobrevin 2-SNAP-25-syntaxin-1a complex Source: MGI
  13. synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex Source: MGI
  14. synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 288288Syntaxin-1APRO_0000210187Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei14 – 141Phosphoserine1 Publication
Modified residuei188 – 1881Phosphoserine; by DAPK1By similarity

Post-translational modificationi

Phosphorylated by CK2. Phosphorylation at Ser-188 by DAPK1 significantly decreases its interaction with STXBP1 (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO35526.
PaxDbiO35526.
PRIDEiO35526.

PTM databases

PhosphoSiteiO35526.

Expressioni

Gene expression databases

BgeeiO35526.
CleanExiMM_STX1A.
ExpressionAtlasiO35526. baseline and differential.
GenevestigatoriO35526.

Interactioni

Subunit structurei

Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Interacts with VAPA and SYBU. Binds STXBP6. Found in a complex with VAMP8 and SNAP23. Found in a ternary complex with STX1A and SNAP25 (By similarity). Interacts with SLC6A4 (By similarity). Binds SYTL4. Interacts with OTOF and LGI3. Interacts with SYT6 and SYT8; the interaction is Ca2+-dependent. Interacts with STXBP1 and DAPK1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Syt2P460972EBI-400878,EBI-457969

Protein-protein interaction databases

BioGridi203561. 16 interactions.
DIPiDIP-31950N.
IntActiO35526. 17 interactions.
MINTiMINT-2411610.

Structurei

3D structure databases

ProteinModelPortaliO35526.
SMRiO35526. Positions 27-286.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini192 – 25463t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili68 – 10942Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the syntaxin family.Curated
Contains 1 t-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5074.
HOGENOMiHOG000286023.
HOVERGENiHBG000497.
InParanoidiO35526.
KOiK04560.
OMAiQIDYRER.
OrthoDBiEOG7X9G7R.
PhylomeDBiO35526.
TreeFamiTF313763.

Family and domain databases

InterProiIPR028669. STX1A/1B.
IPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PANTHERiPTHR19957:SF84. PTHR19957:SF84. 1 hit.
PfamiPF05739. SNARE. 1 hit.
PF00804. Syntaxin. 1 hit.
[Graphical view]
SMARTiSM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35526-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKDRTQELRT AKDSDDDDDV TVTVDRDRFM DEFFEQVEEI RGFIDKIAEN
60 70 80 90 100
VEEVKRKHSA ILASPNPDEK TKEELEELMS DIKKTANKVR SKLKSIEQSI
110 120 130 140 150
EQEEGLNRSS ADLRIRKTQH STLSRKFVEV MSEYNATQSD YRERCKGRIQ
160 170 180 190 200
RQLEITGRTT TSEELEDMLE SGNPAIFASG IIMDSSISKQ ALSEIETRHS
210 220 230 240 250
EIIKLETSIR ELHDMFMDMA MLVESQGEMI DRIEYNVEHA VDYVERAVSD
260 270 280
TKKAVKYQSK ARRKKIMIII CCVILGIIIA STIGGIFG
Length:288
Mass (Da):33,054
Last modified:December 19, 2001 - v3
Checksum:i8F8255F2EEF886CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D45208 mRNA. Translation: BAA28865.2.
CCDSiCCDS19731.1.
RefSeqiNP_058081.2. NM_016801.3.
UniGeneiMm.6225.

Genome annotation databases

EnsembliENSMUST00000005509; ENSMUSP00000005509; ENSMUSG00000007207.
GeneIDi20907.
KEGGimmu:20907.
UCSCiuc008zxk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D45208 mRNA. Translation: BAA28865.2.
CCDSiCCDS19731.1.
RefSeqiNP_058081.2. NM_016801.3.
UniGeneiMm.6225.

3D structure databases

ProteinModelPortaliO35526.
SMRiO35526. Positions 27-286.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203561. 16 interactions.
DIPiDIP-31950N.
IntActiO35526. 17 interactions.
MINTiMINT-2411610.

PTM databases

PhosphoSiteiO35526.

Proteomic databases

MaxQBiO35526.
PaxDbiO35526.
PRIDEiO35526.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005509; ENSMUSP00000005509; ENSMUSG00000007207.
GeneIDi20907.
KEGGimmu:20907.
UCSCiuc008zxk.1. mouse.

Organism-specific databases

CTDi6804.
MGIiMGI:109355. Stx1a.

Phylogenomic databases

eggNOGiCOG5074.
HOGENOMiHOG000286023.
HOVERGENiHBG000497.
InParanoidiO35526.
KOiK04560.
OMAiQIDYRER.
OrthoDBiEOG7X9G7R.
PhylomeDBiO35526.
TreeFamiTF313763.

Enzyme and pathway databases

ReactomeiREACT_205038. Acetylcholine Neurotransmitter Release Cycle.
REACT_229514. Glutamate Neurotransmitter Release Cycle.
REACT_242222. Norepinephrine Neurotransmitter Release Cycle.
REACT_245631. GABA synthesis, release, reuptake and degradation.
REACT_263148. Regulation of insulin secretion.

Miscellaneous databases

NextBioi299781.
PROiO35526.
SOURCEiSearch...

Gene expression databases

BgeeiO35526.
CleanExiMM_STX1A.
ExpressionAtlasiO35526. baseline and differential.
GenevestigatoriO35526.

Family and domain databases

InterProiIPR028669. STX1A/1B.
IPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PANTHERiPTHR19957:SF84. PTHR19957:SF84. 1 hit.
PfamiPF05739. SNARE. 1 hit.
PF00804. Syntaxin. 1 hit.
[Graphical view]
SMARTiSM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Fujiwara T., Genda M., Nagai A., Okazaki M., Watanabe T., Nagamatsu S., Akagawa K.
    Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 29-41; 47-56; 58-84; 95-108; 126-142; 152-158 AND 233-246, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.
  3. "Granuphilin modulates the exocytosis of secretory granules through interaction with syntaxin 1a."
    Torii S., Zhao S., Yi Z., Takeuchi T., Izumi T.
    Mol. Cell. Biol. 22:5518-5526(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SYTL4.
  4. "Synaptotagmin VI and VIII and syntaxin 2 are essential for the mouse sperm acrosome reaction."
    Hutt D.M., Baltz J.M., Ngsee J.K.
    J. Biol. Chem. 280:20197-20203(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SYT6 AND SYT8.
  5. "Otoferlin, defective in a human deafness form, is essential for exocytosis at the auditory ribbon synapse."
    Roux I., Safieddine S., Nouvian R., Grati M., Simmler M.-C., Bahloul A., Perfettini I., Le Gall M., Rostaing P., Hamard G., Triller A., Avan P., Moser T., Petit C.
    Cell 127:277-289(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH OTOF.
    Strain: BALB/c.
    Tissue: Cochlea.
  6. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  7. "Leucine-rich glioma inactivated 3 associates with syntaxin 1."
    Park W.-J., Lee S.E., Kwon N.S., Baek K.J., Kim D.-S., Yun H.-Y.
    Neurosci. Lett. 444:240-244(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH LGI3.

Entry informationi

Entry nameiSTX1A_MOUSE
AccessioniPrimary (citable) accession number: O35526
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 19, 2001
Last modified: February 4, 2015
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.