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Protein

Syntaxin-1A

Gene

Stx1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in hormone and neurotransmitter exocytosis (By similarity). Potentially involved in docking of synaptic vesicles at presynaptic active zones. May mediate Ca2+-regulation of exocytosis acrosomal reaction in sperm (PubMed:15774481).By similarity1 Publication

GO - Molecular functioni

  • calcium channel inhibitor activity Source: MGI
  • chloride channel inhibitor activity Source: MGI
  • glycoprotein binding Source: MGI
  • kinase binding Source: MGI
  • myosin binding Source: GO_Central
  • SNAP receptor activity Source: GO_Central
  • SNARE binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Exocytosis, Neurotransmitter transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-181430. Norepinephrine Neurotransmitter Release Cycle.
R-MMU-210500. Glutamate Neurotransmitter Release Cycle.
R-MMU-264642. Acetylcholine Neurotransmitter Release Cycle.
R-MMU-422356. Regulation of insulin secretion.
R-MMU-449147. Signaling by Interleukins.
R-MMU-5682910. LGI-ADAM interactions.
R-MMU-888590. GABA synthesis, release, reuptake and degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin-1A
Alternative name(s):
Neuron-specific antigen HPC-1
Gene namesi
Name:Stx1a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:109355. Stx1a.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 265CytoplasmicSequence analysisAdd BLAST265
Transmembranei266 – 286Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini287 – 288ExtracellularSequence analysis2

GO - Cellular componenti

  • actomyosin Source: Ensembl
  • cell junction Source: UniProtKB-KW
  • integral component of membrane Source: GO_Central
  • membrane Source: MGI
  • neuron projection Source: MGI
  • plasma membrane Source: MGI
  • secretory granule Source: Ensembl
  • SNARE complex Source: UniProtKB
  • synaptic vesicle Source: UniProtKB
  • synaptic vesicle membrane Source: MGI
  • synaptobrevin 2-SNAP-25-syntaxin-1a complex Source: MGI
  • synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex Source: MGI
  • synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex Source: Ensembl
  • voltage-gated potassium channel complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002101871 – 288Syntaxin-1AAdd BLAST288

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14PhosphoserineCombined sources1
Modified residuei64PhosphoserineCombined sources1
Modified residuei95PhosphoserineBy similarity1
Modified residuei188Phosphoserine; by DAPK1By similarity1

Post-translational modificationi

Phosphorylated by CK2. Phosphorylation at Ser-188 by DAPK1 significantly decreases its interaction with STXBP1 (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO35526.
PeptideAtlasiO35526.
PRIDEiO35526.

PTM databases

iPTMnetiO35526.
PhosphoSitePlusiO35526.
SwissPalmiO35526.

Expressioni

Gene expression databases

BgeeiENSMUSG00000007207.
CleanExiMM_STX1A.
ExpressionAtlasiO35526. baseline and differential.
GenevisibleiO35526. MM.

Interactioni

Subunit structurei

Interacts (via C-terminus) with KCNB1 (via C-terminus); the interaction increases in a calcium-dependent manner and induces a pore-independent enhancement of exocytosis in neuroendocrine cells, chromaffin cells, pancreatic beta cells and from the soma of dorsal root ganglia (DRG) neurons (By similarity). Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Interacts with VAPA and SYBU. Binds STXBP6. Found in a complex with VAMP8 and SNAP23. Found in a ternary complex with STX1A and SNAP25 (By similarity). Interacts with SLC6A4 (By similarity). Binds SYTL4. Interacts with OTOF and LGI3. Interacts with SYT6 and SYT8; the interaction is Ca2+-dependent. Interacts with STXBP1 and DAPK1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Syt2P460972EBI-400878,EBI-457969

GO - Molecular functioni

  • kinase binding Source: MGI
  • myosin binding Source: GO_Central
  • SNAP receptor activity Source: GO_Central
  • SNARE binding Source: MGI

Protein-protein interaction databases

BioGridi203561. 20 interactors.
DIPiDIP-31950N.
IntActiO35526. 17 interactors.
MINTiMINT-2411610.
STRINGi10090.ENSMUSP00000005509.

Structurei

3D structure databases

ProteinModelPortaliO35526.
SMRiO35526.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini192 – 254t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST63

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili68 – 109Sequence analysisAdd BLAST42

Sequence similaritiesi

Belongs to the syntaxin family.Curated
Contains 1 t-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0810. Eukaryota.
COG5074. LUCA.
GeneTreeiENSGT00760000119200.
HOGENOMiHOG000286023.
HOVERGENiHBG000497.
InParanoidiO35526.
KOiK04560.
OMAiETHVANA.
OrthoDBiEOG091G0EUK.
PhylomeDBiO35526.
TreeFamiTF313763.

Family and domain databases

InterProiIPR028669. STX1A/1B.
IPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PANTHERiPTHR19957:SF84. PTHR19957:SF84. 1 hit.
PfamiPF05739. SNARE. 1 hit.
PF00804. Syntaxin. 1 hit.
[Graphical view]
SMARTiSM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35526-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDRTQELRT AKDSDDDDDV TVTVDRDRFM DEFFEQVEEI RGFIDKIAEN
60 70 80 90 100
VEEVKRKHSA ILASPNPDEK TKEELEELMS DIKKTANKVR SKLKSIEQSI
110 120 130 140 150
EQEEGLNRSS ADLRIRKTQH STLSRKFVEV MSEYNATQSD YRERCKGRIQ
160 170 180 190 200
RQLEITGRTT TSEELEDMLE SGNPAIFASG IIMDSSISKQ ALSEIETRHS
210 220 230 240 250
EIIKLETSIR ELHDMFMDMA MLVESQGEMI DRIEYNVEHA VDYVERAVSD
260 270 280
TKKAVKYQSK ARRKKIMIII CCVILGIIIA STIGGIFG
Length:288
Mass (Da):33,054
Last modified:December 19, 2001 - v3
Checksum:i8F8255F2EEF886CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D45208 mRNA. Translation: BAA28865.2.
CCDSiCCDS19731.1.
RefSeqiNP_058081.2. NM_016801.4.
UniGeneiMm.6225.

Genome annotation databases

EnsembliENSMUST00000005509; ENSMUSP00000005509; ENSMUSG00000007207.
GeneIDi20907.
KEGGimmu:20907.
UCSCiuc008zxk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D45208 mRNA. Translation: BAA28865.2.
CCDSiCCDS19731.1.
RefSeqiNP_058081.2. NM_016801.4.
UniGeneiMm.6225.

3D structure databases

ProteinModelPortaliO35526.
SMRiO35526.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203561. 20 interactors.
DIPiDIP-31950N.
IntActiO35526. 17 interactors.
MINTiMINT-2411610.
STRINGi10090.ENSMUSP00000005509.

PTM databases

iPTMnetiO35526.
PhosphoSitePlusiO35526.
SwissPalmiO35526.

Proteomic databases

PaxDbiO35526.
PeptideAtlasiO35526.
PRIDEiO35526.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005509; ENSMUSP00000005509; ENSMUSG00000007207.
GeneIDi20907.
KEGGimmu:20907.
UCSCiuc008zxk.1. mouse.

Organism-specific databases

CTDi6804.
MGIiMGI:109355. Stx1a.

Phylogenomic databases

eggNOGiKOG0810. Eukaryota.
COG5074. LUCA.
GeneTreeiENSGT00760000119200.
HOGENOMiHOG000286023.
HOVERGENiHBG000497.
InParanoidiO35526.
KOiK04560.
OMAiETHVANA.
OrthoDBiEOG091G0EUK.
PhylomeDBiO35526.
TreeFamiTF313763.

Enzyme and pathway databases

ReactomeiR-MMU-181430. Norepinephrine Neurotransmitter Release Cycle.
R-MMU-210500. Glutamate Neurotransmitter Release Cycle.
R-MMU-264642. Acetylcholine Neurotransmitter Release Cycle.
R-MMU-422356. Regulation of insulin secretion.
R-MMU-449147. Signaling by Interleukins.
R-MMU-5682910. LGI-ADAM interactions.
R-MMU-888590. GABA synthesis, release, reuptake and degradation.

Miscellaneous databases

PROiO35526.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000007207.
CleanExiMM_STX1A.
ExpressionAtlasiO35526. baseline and differential.
GenevisibleiO35526. MM.

Family and domain databases

InterProiIPR028669. STX1A/1B.
IPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PANTHERiPTHR19957:SF84. PTHR19957:SF84. 1 hit.
PfamiPF05739. SNARE. 1 hit.
PF00804. Syntaxin. 1 hit.
[Graphical view]
SMARTiSM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTX1A_MOUSE
AccessioniPrimary (citable) accession number: O35526
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 19, 2001
Last modified: November 2, 2016
This is version 151 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.