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O35495 (CDK14_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase 14

EC=2.7.11.22
Alternative name(s):
Cell division protein kinase 14
Serine/threonine-protein kinase PFTAIRE-1
Gene names
Name:Cdk14
Synonyms:Kiaa0834, Pftk1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length469 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Serine/threonine-protein kinase involved in the control of the eukaryotic cell cycle, whose activity is controlled by an associated cyclin. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by mediating the phosphorylation of LRP6 at 'Ser-1490', leading to the activation of the Wnt signaling pathway. Acts as a regulator of cell cycle progression and cell proliferation via its interaction with CCDN3. Phosphorylates RB1 in vitro, however the relevance of such result remains to be confirmed in vivo. May also play a role in meiosis, neuron differentiation and may indirectly act as a negative regulator of insulin-responsive glucose transport By similarity. Ref.1

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Serine/threonine-protein kinase activity is promoted by associated cyclins CCDN3 and CCNY and repressed by CDKN1A By similarity.

Subunit structure

Interacts with CCNY; CCNY mediates its recruitment to the plasma membrane and promotes phosphorylation of LRP6. Interacts with CCDN3 and CDKN1A. Interacts with SEPT8. Interacts with 14-3-3 proteina YWHAB, YWHAE, YWHAH and YWHAQ By similarity.

Subcellular location

Cell membrane; Peripheral membrane protein By similarity. Cytoplasm. Nucleus. Note: Recruited to the cell membrane by CCNY By similarity. Ref.1 Ref.2

Tissue specificity

In the adult, widely expressed at low levels except in brain, kidney and testis where expression is high. In the brain, detected in cortex, hippocampus, dentate gyrus, amygdala cortex, parasubiculum and cerebellum. In the embryo, expressed predominantly in the nervous system. Ref.1

Developmental stage

In the testis, expressed at low levels in Sertoli cells of 7-day-old mice, barely detected at day 17, and detected at much higher levels in late pachytene/diplotene spermatocytes in the adult. In the nervous system, expressed at highest levels in the adult. Ref.1 Ref.2

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Wnt signaling pathway
   Cellular componentCell membrane
Cytoplasm
Membrane
Nucleus
   Coding sequence diversityAlternative splicing
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG2/M transition of mitotic cell cycle

Inferred from sequence or structural similarity. Source: UniProtKB

Wnt signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

cell division

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of canonical Wnt signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from direct assay Ref.2. Source: MGI

cytoplasmic cyclin-dependent protein kinase holoenzyme complex

Inferred from sequence or structural similarity. Source: UniProtKB

cytosol

Inferred from direct assay Ref.2. Source: MGI

nucleus

Inferred from direct assay Ref.2. Source: MGI

plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

cyclin-dependent protein serine/threonine kinase activity

Inferred from sequence or structural similarity. Source: UniProtKB

protein kinase activity

Inferred from direct assay Ref.2. Source: MGI

protein serine/threonine kinase activity

Inferred from sequence or structural similarity Ref.1. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O35495-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O35495-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-46: Missing.
Isoform 3 (identifier: O35495-3)

The sequence of this isoform differs from the canonical sequence as follows:
     349-469: LGTPNEDTWP...YGKSLSNSKH → SSAQSASQKNLFLISYLVT
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 469469Cyclin-dependent kinase 14
PRO_0000086507

Regions

Domain135 – 419285Protein kinase
Nucleotide binding141 – 1499ATP By similarity

Sites

Active site2561Proton acceptor By similarity
Binding site1641ATP By similarity

Amino acid modifications

Modified residue951Phosphoserine By similarity

Natural variations

Alternative sequence1 – 4646Missing in isoform 2.
VSP_038763
Alternative sequence349 – 469121LGTPN…SNSKH → SSAQSASQKNLFLISYLVT in isoform 3.
VSP_038764

Experimental info

Sequence conflict2191Q → R in BAE38049. Ref.3
Sequence conflict222 – 2232DK → EQ in AAB87504. Ref.1
Sequence conflict3751S → N in AAB70455. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 2, 2010. Version 2.
Checksum: 2042D0A66655A8B0

FASTA46952,996
        10         20         30         40         50         60 
MCDLIEPQPA EKIGKMKKLR RTLSESFSRI ALKKEDTTFD EICVTKMSTR NCQGTDSVIK 

        70         80         90        100        110        120 
HLDTIPEDKK VRVQRTQSTF DPFEKPANQV KRVHSENNAC INFKSSSAGK ESPKVRRHSS 

       130        140        150        160        170        180 
PSSPTSPKFG KADSYEKLEK LGEGSYATVY KGKSKVNGKL VALKVIRLQE EEGTPFTAIR 

       190        200        210        220        230        240 
EASLLKGLKH ANIVLLHDII HTKETLTLVF EYVHTDLCQY MDKHPGGLHP DNVKLFLFQL 

       250        260        270        280        290        300 
LRGLSYIHQR YILHRDLKPQ NLLISDTGEL KLADFGLARA KSVPSHTYSN EVVTLWYRPP 

       310        320        330        340        350        360 
DVLLGSTEYS TCLDMWGVGC IFVEMIQGVA AFPGMKDIQD QLERIFLVLG TPNEDTWPGV 

       370        380        390        400        410        420 
HSLPHFKPER FTVYSSKSLR QAWNKLSYVN HAEDLASKLL QCSPKNRLSA QAALSHEYFS 

       430        440        450        460 
DLPPRLWELT DMSSIFTVPN VRLQPEAGES MRAFGKNNSY GKSLSNSKH 

« Hide

Isoform 2 [UniParc].

Checksum: 5895A066A7C17AAB
Show »

FASTA42347,684
Isoform 3 [UniParc].

Checksum: 16A34E81C94C526B
Show »

FASTA36741,378

References

« Hide 'large scale' references
[1]"The identification and characterization of expression of Pftaire-1, a novel Cdk family member, suggest its function in the mouse testis and nervous system."
Besset V., Rhee K., Wolgemuth D.J.
Mol. Reprod. Dev. 50:18-29(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Tissue: Testis.
[2]"A novel cdc2-related protein kinase expressed in the nervous system."
Lazzaro M.A., Albert P.R., Julien J.-P.
J. Neurochem. 69:348-364(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
Strain: NIH Swiss.
Tissue: Brain.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Strain: C57BL/6J.
Tissue: Olfactory bulb.
[4]"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 17-469 (ISOFORM 1).
Tissue: Embryonic tail.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF033655 mRNA. Translation: AAB87504.1.
U62391 mRNA. Translation: AAB70455.1.
AK165149 mRNA. Translation: BAE38049.1.
AK051283 mRNA. Translation: BAE43346.1.
AK129226 mRNA. Translation: BAC98036.1.
RefSeqNP_035204.2. NM_011074.2.
UniGeneMm.389061.
Mm.404502.

3D structure databases

ProteinModelPortalO35495.
SMRO35495. Positions 98-459.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202129. 4 interactions.
IntActO35495. 1 interaction.
MINTMINT-7147773.
STRING10090.ENSMUSP00000030763.

PTM databases

PhosphoSiteO35495.

Proteomic databases

PaxDbO35495.
PRIDEO35495.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000030763; ENSMUSP00000030763; ENSMUSG00000028926. [O35495-1]
ENSMUST00000115450; ENSMUSP00000111110; ENSMUSG00000028926. [O35495-2]
ENSMUST00000115451; ENSMUSP00000111111; ENSMUSG00000028926. [O35495-2]
GeneID18647.
KEGGmmu:18647.
UCSCuc008wih.2. mouse. [O35495-1]
uc008wik.2. mouse. [O35495-3]

Organism-specific databases

CTD5218.
MGIMGI:894318. Cdk14.
RougeSearch...

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00600000083998.
HOGENOMHOG000233024.
HOVERGENHBG014652.
InParanoidQ3TNN9.
KOK08821.
OMAKKLRQAW.
OrthoDBEOG7966H8.
TreeFamTF106508.

Gene expression databases

ArrayExpressO35495.
BgeeO35495.
CleanExMM_PFTK1.
GenevestigatorO35495.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio294648.
PROO35495.
SOURCESearch...

Entry information

Entry nameCDK14_MOUSE
AccessionPrimary (citable) accession number: O35495
Secondary accession number(s): O35848 expand/collapse secondary AC list , Q3TNN9, Q3V319, Q6ZQ37
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: March 2, 2010
Last modified: April 16, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot