O35495 (CDK14_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase 14
EC=2.7.11.22

Alternative name(s):
Cell division protein kinase 14
Serine/threonine-protein kinase PFTAIRE-1

Gene names

Name:	Cdk14
Synonyms:	Kiaa0834, Pftk1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Serine/threonine-protein kinase involved in the control of the eukaryotic cell cycle, whose activity is controlled by an associated cyclin. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by mediating the phosphorylation of LRP6 at 'Ser-1490', leading to the activation of the Wnt signaling pathway. Acts as a regulator of cell cycle progression and cell proliferation via its interaction with CCDN3. Phosphorylates RB1 in vitro, however the relevance of such result remains to be confirmed in vivo. May also play a role in meiosis, neuron differentiation and may indirectly act as a negative regulator of insulin-responsive glucose transport By similarity. Ref.1
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Serine/threonine-protein kinase activity is promoted by associated cyclins CCDN3 and CCNY and repressed by CDKN1A By similarity.
Subunit structure	Interacts with CCNY; CCNY mediates its recruitment to the plasma membrane and promotes phosphorylation of LRP6. Interacts with CCDN3 and CDKN1A. Interacts with SEPT8. Interacts with 14-3-3 proteina YWHAB, YWHAE, YWHAH and YWHAQ By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity. Cytoplasm. Nucleus. Note: Recruited to the cell membrane by CCNY By similarity. Ref.1 Ref.2
Tissue specificity	In the adult, widely expressed at low levels except in brain, kidney and testis where expression is high. In the brain, detected in cortex, hippocampus, dentate gyrus, amgdala cortex, parasubiculum and cerebellum. In the embryo, expressed predominantly in the nervous system. Ref.1
Developmental stage	In the testis, expressed at low levels in Sertoli cells of 7-day-old mice, barely detected at day 17, and detected at much higher levels in late pachytene/diplotene spermatocytes in the adult. In the nervous system, expressed at highest levels in the adult. Ref.1 Ref.2
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Wnt signaling pathway
Cellular component	Cell membrane Cytoplasm Membrane Nucleus
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G2/M transition of mitotic cell cycle Inferred from sequence or structural similarity. Source: UniProtKB Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW regulation of canonical Wnt receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasmic cyclin-dependent protein kinase holoenzyme complex Inferred from sequence or structural similarity. Source: UniProtKB cytosol Inferred from direct assay Ref.2. Source: MGI nucleus Inferred from direct assay Ref.2. Source: MGI plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cyclin-dependent protein serine/threonine kinase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O35495-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O35495-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-46: Missing.

Isoform 3 (identifier: O35495-3)

The sequence of this isoform differs from the canonical sequence as follows:
     349-469: LGTPNEDTWP...YGKSLSNSKH → SSAQSASQKNLFLISYLVT

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	Cyclin-dependent kinase 14		PRO_0000086507
Regions
	Domain	135 – 419	285	Protein kinase
	Nucleotide binding	141 – 149	9	ATP By similarity
Sites
	Active site	256	1	Proton acceptor By similarity
	Binding site	164	1	ATP By similarity
Amino acid modifications
	Modified residue	95	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 46	46	Missing in isoform 2.		VSP_038763
	Alternative sequence	349 – 469	121	LGTPN…SNSKH → SSAQSASQKNLFLISYLVT in isoform 3.		VSP_038764
Experimental info
	Sequence conflict	219	1	Q → R in BAE38049. Ref.3
	Sequence conflict	222 – 223	2	DK → EQ in AAB87504. Ref.1
	Sequence conflict	375	1	S → N in AAB70455. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 2, 2010. Version 2. Checksum: 2042D0A66655A8B0	FASTA	469	52,996
	10 20 30 40 50 60 MCDLIEPQPA EKIGKMKKLR RTLSESFSRI ALKKEDTTFD EICVTKMSTR NCQGTDSVIK 70 80 90 100 110 120 HLDTIPEDKK VRVQRTQSTF DPFEKPANQV KRVHSENNAC INFKSSSAGK ESPKVRRHSS 130 140 150 160 170 180 PSSPTSPKFG KADSYEKLEK LGEGSYATVY KGKSKVNGKL VALKVIRLQE EEGTPFTAIR 190 200 210 220 230 240 EASLLKGLKH ANIVLLHDII HTKETLTLVF EYVHTDLCQY MDKHPGGLHP DNVKLFLFQL 250 260 270 280 290 300 LRGLSYIHQR YILHRDLKPQ NLLISDTGEL KLADFGLARA KSVPSHTYSN EVVTLWYRPP 310 320 330 340 350 360 DVLLGSTEYS TCLDMWGVGC IFVEMIQGVA AFPGMKDIQD QLERIFLVLG TPNEDTWPGV 370 380 390 400 410 420 HSLPHFKPER FTVYSSKSLR QAWNKLSYVN HAEDLASKLL QCSPKNRLSA QAALSHEYFS 430 440 450 460 DLPPRLWELT DMSSIFTVPN VRLQPEAGES MRAFGKNNSY GKSLSNSKH
	Isoform 2 [UniParc]. Checksum: 5895A066A7C17AAB	FASTA	423	47,684
	10 20 30 40 50 60 MSTRNCQGTD SVIKHLDTIP EDKKVRVQRT QSTFDPFEKP ANQVKRVHSE NNACINFKSS 70 80 90 100 110 120 SAGKESPKVR RHSSPSSPTS PKFGKADSYE KLEKLGEGSY ATVYKGKSKV NGKLVALKVI 130 140 150 160 170 180 RLQEEEGTPF TAIREASLLK GLKHANIVLL HDIIHTKETL TLVFEYVHTD LCQYMDKHPG 190 200 210 220 230 240 GLHPDNVKLF LFQLLRGLSY IHQRYILHRD LKPQNLLISD TGELKLADFG LARAKSVPSH 250 260 270 280 290 300 TYSNEVVTLW YRPPDVLLGS TEYSTCLDMW GVGCIFVEMI QGVAAFPGMK DIQDQLERIF 310 320 330 340 350 360 LVLGTPNEDT WPGVHSLPHF KPERFTVYSS KSLRQAWNKL SYVNHAEDLA SKLLQCSPKN 370 380 390 400 410 420 RLSAQAALSH EYFSDLPPRL WELTDMSSIF TVPNVRLQPE AGESMRAFGK NNSYGKSLSN SKH
	Isoform 3 [UniParc]. Checksum: 16A34E81C94C526B	FASTA	367	41,378
	10 20 30 40 50 60 MCDLIEPQPA EKIGKMKKLR RTLSESFSRI ALKKEDTTFD EICVTKMSTR NCQGTDSVIK 70 80 90 100 110 120 HLDTIPEDKK VRVQRTQSTF DPFEKPANQV KRVHSENNAC INFKSSSAGK ESPKVRRHSS 130 140 150 160 170 180 PSSPTSPKFG KADSYEKLEK LGEGSYATVY KGKSKVNGKL VALKVIRLQE EEGTPFTAIR 190 200 210 220 230 240 EASLLKGLKH ANIVLLHDII HTKETLTLVF EYVHTDLCQY MDKHPGGLHP DNVKLFLFQL 250 260 270 280 290 300 LRGLSYIHQR YILHRDLKPQ NLLISDTGEL KLADFGLARA KSVPSHTYSN EVVTLWYRPP 310 320 330 340 350 360 DVLLGSTEYS TCLDMWGVGC IFVEMIQGVA AFPGMKDIQD QLERIFLVSS AQSASQKNLF LISYLVT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The identification and characterization of expression of Pftaire-1, a novel Cdk family member, suggest its function in the mouse testis and nervous system." Besset V., Rhee K., Wolgemuth D.J. Mol. Reprod. Dev. 50:18-29(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Testis.
[2]	"A novel cdc2-related protein kinase expressed in the nervous system." Lazzaro M.A., Albert P.R., Julien J.-P. J. Neurochem. 69:348-364(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE. Strain: NIH Swiss. Tissue: Brain.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Strain: C57BL/6J. Tissue: Olfactory bulb.
[4]	"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H. DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 17-469 (ISOFORM 1). Tissue: Embryonic tail.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF033655 mRNA. Translation: AAB87504.1. U62391 mRNA. Translation: AAB70455.1. AK165149 mRNA. Translation: BAE38049.1. AK051283 mRNA. Translation: BAE43346.1. AK129226 mRNA. Translation: BAC98036.1.
IPI	IPI00130957. IPI00856237. IPI00955356.
RefSeq	NP_035204.2. NM_011074.2.
UniGene	Mm.389061. Mm.404502.
3D structure databases
ProteinModelPortal	O35495.
SMR	O35495. Positions 98-447.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-7147773.
STRING	10090.ENSMUSP00000030763.
PTM databases
PhosphoSite	O35495.
Proteomic databases
PaxDb	O35495.
PRIDE	O35495.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000030763; ENSMUSP00000030763; ENSMUSG00000028926. ENSMUST00000115450; ENSMUSP00000111110; ENSMUSG00000028926. ENSMUST00000115451; ENSMUSP00000111111; ENSMUSG00000028926.
GeneID	18647.
KEGG	mmu:18647.
UCSC	uc008wii.2. mouse. uc008wik.2. mouse.
Organism-specific databases
CTD	5218.
MGI	MGI:894318. Cdk14.
Rouge	Search...
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00600000083998.
HOGENOM	HOG000233024.
HOVERGEN	HBG014652.
InParanoid	Q3TNN9.
KO	K08821.
OMA	KPERFTQ.
Gene expression databases
ArrayExpress	O35495.
Bgee	O35495.
CleanEx	MM_PFTK1.
Genevestigator	O35495.
GermOnline	ENSMUSG00000028926. Mus musculus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	294648.
SOURCE	Search...

Entry information

Entry name

CDK14_MOUSE

Accession

Primary (citable) accession number: O35495
Secondary accession number(s): O35848 , Q3TNN9, Q3V319, Q6ZQ37

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	March 2, 2010
Last modified:	May 1, 2013
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program