Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O35495

- CDK14_MOUSE

UniProt

O35495 - CDK14_MOUSE

Protein

Cyclin-dependent kinase 14

Gene

Cdk14

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 2 (02 Mar 2010)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Serine/threonine-protein kinase involved in the control of the eukaryotic cell cycle, whose activity is controlled by an associated cyclin. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by mediating the phosphorylation of LRP6 at 'Ser-1490', leading to the activation of the Wnt signaling pathway. Acts as a regulator of cell cycle progression and cell proliferation via its interaction with CCDN3. Phosphorylates RB1 in vitro, however the relevance of such result remains to be confirmed in vivo. May also play a role in meiosis, neuron differentiation and may indirectly act as a negative regulator of insulin-responsive glucose transport By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Serine/threonine-protein kinase activity is promoted by associated cyclins CCDN3 and CCNY and repressed by CDKN1A.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei164 – 1641ATPPROSITE-ProRule annotation
    Active sitei256 – 2561Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi141 – 1499ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
    3. protein kinase activity Source: MGI
    4. protein serine/threonine kinase activity Source: MGI

    GO - Biological processi

    1. cell division Source: UniProtKB-KW
    2. G2/M transition of mitotic cell cycle Source: UniProtKB
    3. regulation of canonical Wnt signaling pathway Source: UniProtKB
    4. regulation of cell cycle Source: GOC
    5. Wnt signaling pathway Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division, Wnt signaling pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase 14 (EC:2.7.11.22)
    Alternative name(s):
    Cell division protein kinase 14
    Serine/threonine-protein kinase PFTAIRE-1
    Gene namesi
    Name:Cdk14
    Synonyms:Kiaa0834, Pftk1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:894318. Cdk14.

    Subcellular locationi

    Cell membrane By similarity; Peripheral membrane protein By similarity. Cytoplasm. Nucleus
    Note: Recruited to the cell membrane by CCNY.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. cytoplasmic cyclin-dependent protein kinase holoenzyme complex Source: UniProtKB
    3. cytosol Source: MGI
    4. nucleus Source: MGI
    5. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 469469Cyclin-dependent kinase 14PRO_0000086507Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei95 – 951PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiO35495.
    PaxDbiO35495.
    PRIDEiO35495.

    PTM databases

    PhosphoSiteiO35495.

    Expressioni

    Tissue specificityi

    In the adult, widely expressed at low levels except in brain, kidney and testis where expression is high. In the brain, detected in cortex, hippocampus, dentate gyrus, amygdala cortex, parasubiculum and cerebellum. In the embryo, expressed predominantly in the nervous system.1 Publication

    Developmental stagei

    In the testis, expressed at low levels in Sertoli cells of 7-day-old mice, barely detected at day 17, and detected at much higher levels in late pachytene/diplotene spermatocytes in the adult. In the nervous system, expressed at highest levels in the adult.2 Publications

    Gene expression databases

    ArrayExpressiO35495.
    BgeeiO35495.
    CleanExiMM_PFTK1.
    GenevestigatoriO35495.

    Interactioni

    Subunit structurei

    Interacts with CCNY; CCNY mediates its recruitment to the plasma membrane and promotes phosphorylation of LRP6. Interacts with CCDN3 and CDKN1A. Interacts with SEPT8. Interacts with 14-3-3 proteina YWHAB, YWHAE, YWHAH and YWHAQ By similarity.By similarity

    Protein-protein interaction databases

    BioGridi202129. 4 interactions.
    IntActiO35495. 1 interaction.
    MINTiMINT-7147773.
    STRINGi10090.ENSMUSP00000030763.

    Structurei

    3D structure databases

    ProteinModelPortaliO35495.
    SMRiO35495. Positions 98-459.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini135 – 419285Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00600000083998.
    HOGENOMiHOG000233024.
    HOVERGENiHBG014652.
    InParanoidiQ3TNN9.
    KOiK08821.
    OMAiKKLRQAW.
    OrthoDBiEOG7966H8.
    TreeFamiTF106508.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O35495-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MCDLIEPQPA EKIGKMKKLR RTLSESFSRI ALKKEDTTFD EICVTKMSTR    50
    NCQGTDSVIK HLDTIPEDKK VRVQRTQSTF DPFEKPANQV KRVHSENNAC 100
    INFKSSSAGK ESPKVRRHSS PSSPTSPKFG KADSYEKLEK LGEGSYATVY 150
    KGKSKVNGKL VALKVIRLQE EEGTPFTAIR EASLLKGLKH ANIVLLHDII 200
    HTKETLTLVF EYVHTDLCQY MDKHPGGLHP DNVKLFLFQL LRGLSYIHQR 250
    YILHRDLKPQ NLLISDTGEL KLADFGLARA KSVPSHTYSN EVVTLWYRPP 300
    DVLLGSTEYS TCLDMWGVGC IFVEMIQGVA AFPGMKDIQD QLERIFLVLG 350
    TPNEDTWPGV HSLPHFKPER FTVYSSKSLR QAWNKLSYVN HAEDLASKLL 400
    QCSPKNRLSA QAALSHEYFS DLPPRLWELT DMSSIFTVPN VRLQPEAGES 450
    MRAFGKNNSY GKSLSNSKH 469
    Length:469
    Mass (Da):52,996
    Last modified:March 2, 2010 - v2
    Checksum:i2042D0A66655A8B0
    GO
    Isoform 2 (identifier: O35495-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-46: Missing.

    Show »
    Length:423
    Mass (Da):47,684
    Checksum:i5895A066A7C17AAB
    GO
    Isoform 3 (identifier: O35495-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         349-469: LGTPNEDTWP...YGKSLSNSKH → SSAQSASQKNLFLISYLVT

    Note: No experimental confirmation available.

    Show »
    Length:367
    Mass (Da):41,378
    Checksum:i16A34E81C94C526B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti219 – 2191Q → R in BAE38049. (PubMed:16141072)Curated
    Sequence conflicti222 – 2232DK → EQ in AAB87504. (PubMed:9547506)Curated
    Sequence conflicti375 – 3751S → N in AAB70455. (PubMed:9202329)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 4646Missing in isoform 2. 2 PublicationsVSP_038763Add
    BLAST
    Alternative sequencei349 – 469121LGTPN…SNSKH → SSAQSASQKNLFLISYLVT in isoform 3. 1 PublicationVSP_038764Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF033655 mRNA. Translation: AAB87504.1.
    U62391 mRNA. Translation: AAB70455.1.
    AK165149 mRNA. Translation: BAE38049.1.
    AK051283 mRNA. Translation: BAE43346.1.
    AK129226 mRNA. Translation: BAC98036.1.
    CCDSiCCDS19073.1. [O35495-1]
    RefSeqiNP_035204.2. NM_011074.2. [O35495-1]
    UniGeneiMm.389061.
    Mm.404502.

    Genome annotation databases

    EnsembliENSMUST00000030763; ENSMUSP00000030763; ENSMUSG00000028926. [O35495-1]
    ENSMUST00000115450; ENSMUSP00000111110; ENSMUSG00000028926. [O35495-2]
    ENSMUST00000115451; ENSMUSP00000111111; ENSMUSG00000028926. [O35495-2]
    GeneIDi18647.
    KEGGimmu:18647.
    UCSCiuc008wih.2. mouse. [O35495-1]
    uc008wik.2. mouse. [O35495-3]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF033655 mRNA. Translation: AAB87504.1 .
    U62391 mRNA. Translation: AAB70455.1 .
    AK165149 mRNA. Translation: BAE38049.1 .
    AK051283 mRNA. Translation: BAE43346.1 .
    AK129226 mRNA. Translation: BAC98036.1 .
    CCDSi CCDS19073.1. [O35495-1 ]
    RefSeqi NP_035204.2. NM_011074.2. [O35495-1 ]
    UniGenei Mm.389061.
    Mm.404502.

    3D structure databases

    ProteinModelPortali O35495.
    SMRi O35495. Positions 98-459.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202129. 4 interactions.
    IntActi O35495. 1 interaction.
    MINTi MINT-7147773.
    STRINGi 10090.ENSMUSP00000030763.

    PTM databases

    PhosphoSitei O35495.

    Proteomic databases

    MaxQBi O35495.
    PaxDbi O35495.
    PRIDEi O35495.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000030763 ; ENSMUSP00000030763 ; ENSMUSG00000028926 . [O35495-1 ]
    ENSMUST00000115450 ; ENSMUSP00000111110 ; ENSMUSG00000028926 . [O35495-2 ]
    ENSMUST00000115451 ; ENSMUSP00000111111 ; ENSMUSG00000028926 . [O35495-2 ]
    GeneIDi 18647.
    KEGGi mmu:18647.
    UCSCi uc008wih.2. mouse. [O35495-1 ]
    uc008wik.2. mouse. [O35495-3 ]

    Organism-specific databases

    CTDi 5218.
    MGIi MGI:894318. Cdk14.
    Rougei Search...

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00600000083998.
    HOGENOMi HOG000233024.
    HOVERGENi HBG014652.
    InParanoidi Q3TNN9.
    KOi K08821.
    OMAi KKLRQAW.
    OrthoDBi EOG7966H8.
    TreeFami TF106508.

    Miscellaneous databases

    NextBioi 294648.
    PROi O35495.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O35495.
    Bgeei O35495.
    CleanExi MM_PFTK1.
    Genevestigatori O35495.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The identification and characterization of expression of Pftaire-1, a novel Cdk family member, suggest its function in the mouse testis and nervous system."
      Besset V., Rhee K., Wolgemuth D.J.
      Mol. Reprod. Dev. 50:18-29(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Testis.
    2. "A novel cdc2-related protein kinase expressed in the nervous system."
      Lazzaro M.A., Albert P.R., Julien J.-P.
      J. Neurochem. 69:348-364(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
      Strain: NIH Swiss.
      Tissue: Brain.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
      Strain: C57BL/6J.
      Tissue: Olfactory bulb.
    4. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
      DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 17-469 (ISOFORM 1).
      Tissue: Embryonic tail.

    Entry informationi

    Entry nameiCDK14_MOUSE
    AccessioniPrimary (citable) accession number: O35495
    Secondary accession number(s): O35848
    , Q3TNN9, Q3V319, Q6ZQ37
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 30, 2002
    Last sequence update: March 2, 2010
    Last modified: October 1, 2014
    This is version 115 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3