Reviewed,
UniProtKB/Swiss-Prot O35493 (CLK4_MOUSE)
Last modified
November 3, 2009.
Version 75.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Dual specificity protein kinase CLK4 EC=2.7.12.1 Alternative name(s): CDC-like kinase 4 | ||
| Gene names |
| ||
| Organism | Mus musculus (Mouse) | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 481 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates serines, threonines and tyrosines. Required for the regulation of alternative splicing of MAPT/TAU. Ref.5 |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Subunit structure | Interacts with UBL5 By similarity. |
| Subcellular location | Nucleus By similarity. |
| Tissue specificity | Expressed in the hippocampus, the cerebellum and the olfactory bulb. Ref.6 |
| Post-translational modification | Autophosphorylates on all three types of residues. |
| Sequence similarities | Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily. Contains 1 protein kinase domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Nucleus |
| Coding sequence diversity | Alternative splicing |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase |
| PTM | Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | peptidyl-tyrosine phosphorylation Ref.1 Inferred from direct assay. Source: MGI protein amino acid autophosphorylation Ref.1Inferred from direct assay. Source: MGI |
| Cellular component | nucleus Ref.1 Inferred from direct assay. Source: MGI |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activityInferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Ref.1Inferred from direct assay. Source: MGI |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: O35493-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: O35493-2) The sequence of this isoform differs from the canonical sequence as follows: 1-180: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 481 | 481 | Dual specificity protein kinase CLK4 | PRO_0000085874 | |||||
Regions | |||||||||
| Domain | 159 – 475 | 317 | Protein kinase | ||||||
| Nucleotide binding | 165 – 173 | 9 | ATP By similarity | ||||||
Sites | |||||||||
| Active site | 286 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 189 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 136 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 138 | 1 | Phosphoserine By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 180 | 180 | Missing in isoform 2. | VSP_008205 | |||||
Experimental info | |||||||||
| Mutagenesis | 189 | 1 | K → R: Loss of function. Ref.6 | ||||||
| Sequence conflict | 58 | 1 | E → Q in AAB62179. Ref.4 | ||||||
| Sequence conflict | 141 | 1 | D → N in AAB62179. Ref.4 | ||||||
| Sequence conflict | 344 | 1 | R → K in BAC25420. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Characterization and comparison of four serine- and arginine-rich (SR) protein kinases." Nayler O., Stamm S., Ullrich A. Biochem. J. 326:693-700(1997) [PubMed: 9307018] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). |
| [2] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Head. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: Czech II. Tissue: Mammary tumor. |
| [4] | "Construction of a 3-Mb contig and partial transcript map of the central region of mouse chromosome 11." Watkins-Chow D.E., Douglas K.R., Buckwalter M.S., Probst F.J., Camper S.A. Genomics 45:147-157(1997) [PubMed: 9339371] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-219. |
| [5] | "Regulation of alternative splicing of human tau exon 10 by phosphorylation of splicing factors." Hartmann A.M., Rujescu D., Giannakouros T., Nikolakaki E., Goedert M., Mandelkow E.-M., Gao Q.S., Andreadis A., Stamm S. Mol. Cell. Neurosci. 18:80-90(2001) [PubMed: 11461155] [Abstract] Cited for: FUNCTION. |
| [6] | "Novel SR-rich-related protein Clasp specifically interacts with inactivated Clk4 and induces the exon EB inclusion of Clk." Katsu R., Onogi H., Wada K., Kawaguchi Y., Hagiwara M. J. Biol. Chem. 277:44220-44228(2002) [PubMed: 12169693] [Abstract] Cited for: TISSUE SPECIFICITY, MUTAGENESIS OF LYS-189. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AF033566 mRNA. Translation: AAB87510.1. AK013974 mRNA. Translation: BAC25420.1. BC002220 mRNA. Translation: AAH02220.1. BC012675 mRNA. Translation: AAH12675.1. U94846 mRNA. Translation: AAB62179.1. | |
| IPI | IPI00130955. IPI00341291. |
| RefSeq | NP_031740.1. |
| UniGene | Mm.239354 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1BI8 based on UniProtKB Q00534. |
| SMR | O35493. Positions 146-480. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | O35493. |
PTM databases | |
| PhosphoSite | O35493. |
Proteomic databases | |
| PRIDE | O35493. |
Genome annotation databases | |
| Ensembl | ENSMUST00000093132; ENSMUSP00000090820; ENSMUSG00000020385; Mus musculus. [Genome view] |
| GeneID | 12750. |
| KEGG | mmu:12750. |
| UCSC | uc007itr.1. mouse. |
Organism-specific databases | |
| CTD | 12750. |
| MGI | MGI:1098551. Clk4. |
Phylogenomic databases | |
| HOGENOM | O35493. |
| HOVERGEN | O35493. |
| OMA | PVKRITL. |
Enzyme and pathway databases | |
| BRENDA | 2.7.12.1. 244. |
Gene expression databases | |
| ArrayExpress | O35493. |
| Bgee | O35493. |
| CleanEx | MM_CLK4. |
| Genevestigator | O35493. |
| GermOnline | ENSMUSG00000020385. Mus musculus. |
Family and domain databases | |
| InterPro | IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view] |
| Pfam | PF00069. Pkinase. 1 hit. [Graphical view] |
| ProDom | PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00220. S_TKc. 1 hit. [Graphical view] |
| PROSITE | PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 282080. |
| SOURCE | Search... |
Entry information
| Entry name | CLK4_MOUSE | ||||||||
| Accession | Primary (citable) accession number: O35493 Secondary accession number(s): O35721, Q8CEU9, Q99LU6 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| Human and mouse protein kinases Human and mouse protein kinases: classification and index |
| SIMILARITY comments Index of protein domains and families |

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