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Protein

Dual specificity protein kinase CLK4

Gene

Clk4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates SRSF1 and SRSF3. Required for the regulation of alternative splicing of MAPT/TAU. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

TG003 inhibits its kinase activity and affects the regulation of alternative splicing mediated by phosphorylation of SR proteins.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei189ATPPROSITE-ProRule annotation1
Active sitei286Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi165 – 173ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine/tyrosine kinase activity Source: UniProtKB-EC
  • protein serine/threonine kinase activity Source: UniProtKB
  • protein tyrosine kinase activity Source: MGI

GO - Biological processi

  • peptidyl-tyrosine phosphorylation Source: MGI
  • protein autophosphorylation Source: MGI
  • regulation of RNA splicing Source: MGI

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.12.1 3474

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein kinase CLK4 (EC:2.7.12.1)
Alternative name(s):
CDC-like kinase 4
Gene namesi
Name:Clk4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1098551 Clk4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi189K → R: Loss of function. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL1075283
GuidetoPHARMACOLOGYi1993

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000858741 – 481Dual specificity protein kinase CLK4Add BLAST481

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei136PhosphoserineBy similarity1
Modified residuei138PhosphoserineCombined sources1

Post-translational modificationi

Autophosphorylates on all three types of residues.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO35493
PaxDbiO35493
PeptideAtlasiO35493
PRIDEiO35493

PTM databases

iPTMnetiO35493
PhosphoSitePlusiO35493

Expressioni

Tissue specificityi

Expressed in the hippocampus, the cerebellum and the olfactory bulb.1 Publication

Gene expression databases

BgeeiENSMUSG00000020385
CleanExiMM_CLK4
ExpressionAtlasiO35493 baseline and differential
GenevisibleiO35493 MM

Interactioni

Subunit structurei

Interacts with UBL5.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000090820

Chemistry databases

BindingDBiO35493

Structurei

3D structure databases

ProteinModelPortaliO35493
SMRiO35493
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini159 – 475Protein kinasePROSITE-ProRule annotationAdd BLAST317

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0671 Eukaryota
ENOG410XQF2 LUCA
GeneTreeiENSGT00580000081366
HOGENOMiHOG000203417
HOVERGENiHBG107720
InParanoidiO35493
KOiK08823
OMAiHGMDGIH
OrthoDBiEOG091G033A
PhylomeDBiO35493
TreeFamiTF101041

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O35493-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRHSKRTHCP DWDSRESWGH ESYSGSHKRK RRSHSSTQEN RHCKPHHQFK
60 70 80 90 100
DSDCHYLEAR CLNERDYRDR RYIDEYRNDY CEGYVPRHYH RDVESTYRIH
110 120 130 140 150
CSKSSVRSRR SSPKRKRNRP CASHQSHSKS HRRKRSRSIE DDEEGHLICQ
160 170 180 190 200
SGDVLRARYE IVDTLGEGAF GKVVECIDHG MDGLHVAVKI VKNVGRYREA
210 220 230 240 250
ARSEIQVLEH LNSTDPNSVF RCVQMLEWFD HHGHVCIVFE LLGLSTYDFI
260 270 280 290 300
KENSFLPFQI DHIRQMAYQI CQSINFLHHN KLTHTDLKPE NILFVKSDYV
310 320 330 340 350
VKYNSKMKRD ERTLKNTDIK VVDFGSATYD DEHHSTLVST RHYRAPEVIL
360 370 380 390 400
ALGWSQPCDV WSIGCILIEY YLGFTVFQTH DSKEHLAMME RILGPIPAHM
410 420 430 440 450
IQKTRKRKYF HHNQLDWDEH SSAGRYVRRR CKPLKEFMLC HDEEHEKLFD
460 470 480
LVRRMLEYDP ARRITLDEAL QHPFFDLLKR K
Length:481
Mass (Da):57,345
Last modified:January 1, 1998 - v1
Checksum:iF2C56965900C12AA
GO
Isoform 2 (identifier: O35493-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-180: Missing.

Show »
Length:301
Mass (Da):35,768
Checksum:i7F1757E2EF28C6C1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti58E → Q in AAB62179 (PubMed:9339371).Curated1
Sequence conflicti141D → N in AAB62179 (PubMed:9339371).Curated1
Sequence conflicti344R → K in BAC25420 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0082051 – 180Missing in isoform 2. 2 PublicationsAdd BLAST180

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033566 mRNA Translation: AAB87510.1
AK013974 mRNA Translation: BAC25420.1
BC002220 mRNA Translation: AAH02220.1
BC012675 mRNA Translation: AAH12675.1
U94846 mRNA Translation: AAB62179.1
CCDSiCCDS24651.1 [O35493-1]
CCDS78942.1 [O35493-2]
RefSeqiNP_001291673.1, NM_001304744.1 [O35493-2]
NP_031740.1, NM_007714.6 [O35493-1]
XP_006532177.1, XM_006532114.3 [O35493-2]
XP_006532178.1, XM_006532115.3 [O35493-2]
XP_006532179.1, XM_006532116.2 [O35493-2]
XP_006532181.1, XM_006532118.2 [O35493-2]
XP_017169733.1, XM_017314244.1 [O35493-2]
UniGeneiMm.239354

Genome annotation databases

EnsembliENSMUST00000093132; ENSMUSP00000090820; ENSMUSG00000020385 [O35493-1]
ENSMUST00000109111; ENSMUSP00000104739; ENSMUSG00000020385 [O35493-2]
ENSMUST00000109113; ENSMUSP00000104741; ENSMUSG00000020385 [O35493-2]
GeneIDi12750
KEGGimmu:12750
UCSCiuc007itr.2 mouse [O35493-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCLK4_MOUSE
AccessioniPrimary (citable) accession number: O35493
Secondary accession number(s): O35721, Q8CEU9, Q99LU6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: March 28, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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