Reviewed, UniProtKB/Swiss-Prot O35491 (CLK2_MOUSE)

Names and origin

Protein names

Recommended name:
    Dual specificity protein kinase CLK2
    EC=2.7.12.1
Alternative name(s):
    CDC-like kinase 2

Gene names

Name:

Clk2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates serines, threonines and tyrosines.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Nucleus By similarity.
Post-translational modification	Autophosphorylates on all three types of residues.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	peptidyl-tyrosine phosphorylation Ref.1 Inferred from direct assay. Source: MGI protein amino acid autophosphorylation Ref.1 Inferred from direct assay. Source: MGI response to retinoic acid Inferred from direct assay. Source: MGI
Cellular component	nucleus Ref.1 Inferred from direct assay. Source: MGI
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Ref.1 Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Dual specificity protein kinase CLK2		PRO_0000085869
Regions
	Domain	163 – 479	317	Protein kinase
	Nucleotide binding	168 – 176	9	ATP By similarity
Sites
	Active site	289	1	Proton acceptor By similarity
	Binding site	192	1	ATP By similarity
Amino acid modifications
	Modified residue	51	1	Phosphotyrosine By similarity
	Modified residue	98	1	Phosphoserine By similarity
	Modified residue	141	1	Phosphoserine By similarity
	Modified residue	493	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O35491-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 092946BA171C9ADD	FASTA	499	59,955
	10 20 30 40 50 60 MPHPRRYHSS ERGSRGSYHE HYQSRKHKRR RSRSWSSSSD RTRRRRREDS YHVRSRSSYD 70 80 90 100 110 120 DHSSDRRLYD RRYCGSYRRN DYSRDRGEAY YDTDFRQSYE YHRENSSYRS QRSSRRKHRR 130 140 150 160 170 180 RRRRSRTFSR SSSHSSRRAK SVEDDAEGHL IYHVGDWLQE RYEIVSTLGE GTSGRVVQCV 190 200 210 220 230 240 DHRRGGTRVA LKIIKNVEKY KEAARLEINV LEKINEKDPD NKNLCVQMFD WFDYHGHMCI 250 260 270 280 290 300 SFELLGLSTF DFLKDNNYLP YPIHQVRHMA FQLCQAVKFL HDNKLTHTDL KPENILFVNS 310 320 330 340 350 360 DYELTYNLEK KRDERSVKST AVRVVDFGSA TFDHEHHSTI VSTRHYRAPE VILELGWSQP 370 380 390 400 410 420 CDVWSIGCII FEYYVGFTLF QTHDNREHLA MMERILGPVP SRMIRKTRKQ KYFYRGRLDW 430 440 450 460 470 480 DENTSAGRYV RENCKPLRRY LTSEAEDHHQ LFDLIENMLE YEPAKRLTLG EALQHPFFAC 490 LRTEPPNTKL WDSSRDISR

References

[1]	"Characterization and comparison of four serine- and arginine-rich (SR) protein kinases." Nayler O., Stamm S., Ullrich A. Biochem. J. 326:693-700(1997) [PubMed: 9307018] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF033564 mRNA. Translation: AAB87508.1.
IPI	IPI00130951.
UniGene	Mm.288098
3D structure databases
HSSP	HSSP built from PDB template 1HOW based on UniProtKB Q03656.
SMR	O35491. Positions 142-484.
ModBase	Search...
Protein-protein interaction databases
STRING	O35491.
PTM databases
PhosphoSite	O35491.
Proteomic databases
PRIDE	O35491.
Genome annotation databases
Ensembl	ENSMUST00000090927; ENSMUSP00000088445; ENSMUSG00000068917; Mus musculus. [Genome view] ENSMUST00000121931; ENSMUSP00000113861; ENSMUSG00000068917; Mus musculus. [Genome view]
KEGG	mmu:12748.
UCSC	uc008pxt.1. mouse.
Organism-specific databases
CTD	12748.
MGI	MGI:1098669. Clk2.
Phylogenomic databases
HOGENOM	O35491.
HOVERGEN	O35491.
Enzyme and pathway databases
BRENDA	2.7.12.1. 244.
Gene expression databases
ArrayExpress	O35491.
Bgee	O35491.
Genevestigator	O35491.
GermOnline	ENSMUSG00000068917. Mus musculus.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	282076.
SOURCE	Search...

Entry information

Entry name

CLK2_MOUSE

Accession

Primary (citable) accession number: O35491

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	October 13, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)