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Protein

Very long-chain acyl-CoA synthetase

Gene

Slc27a2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acyl-CoA synthetase probably involved in bile acid metabolism. Proposed to activate C27 precursors of bile acids to their CoA thioesters derivatives before side chain cleavage via peroxisomal beta-oxidation occurs. In vitro, activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol. Does not utilize C24 bile acids as substrates. In vitro, also activates long- and branched-chain fatty acids and may have additional roles in fatty acid metabolism (By similarity). May be involved in translocation of long-chain fatty acids (LFCA) across membranes.By similarity1 Publication

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.
ATP + a very-long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi222 – 233AMPSequence analysisAdd BLAST12

GO - Molecular functioni

GO - Biological processi

  • bile acid biosynthetic process Source: MGI
  • fatty acid alpha-oxidation Source: MGI
  • fatty acid beta-oxidation Source: MGI
  • fatty acid transport Source: MGI
  • long-chain fatty acid import Source: MGI
  • long-chain fatty acid metabolic process Source: MGI
  • methyl-branched fatty acid metabolic process Source: MGI
  • very long-chain fatty acid catabolic process Source: MGI
  • very long-chain fatty acid metabolic process Source: MGI

Keywordsi

Molecular functionLigase
Biological processFatty acid metabolism, Lipid metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
R-MMU-193775 Synthesis of bile acids and bile salts via 24-hydroxycholesterol
R-MMU-389599 Alpha-oxidation of phytanate
R-MMU-6798695 Neutrophil degranulation
R-MMU-9033241 Peroxisomal protein import

Chemistry databases

SwissLipidsiSLP:000000430

Names & Taxonomyi

Protein namesi
Recommended name:
Very long-chain acyl-CoA synthetase (EC:6.2.1.-)
Short name:
VLACS
Short name:
VLCS
Alternative name(s):
Fatty acid transport protein 2
Short name:
FATP-2
Fatty-acid-coenzyme A ligase, very long-chain 1
Long-chain-fatty-acid--CoA ligase (EC:6.2.1.3)
Solute carrier family 27 member 2
THCA-CoA ligase
Very long-chain-fatty-acid-CoA ligase
Gene namesi
Name:Slc27a2
Synonyms:Acsvl1, Facvl1, Fatp2, Vlacs, Vlcs
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1347099 Slc27a2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 4LumenalBy similarity4
Transmembranei5 – 27HelicalSequence analysisAdd BLAST23
Topological domaini28 – 106CytoplasmicSequence analysisAdd BLAST79
Transmembranei107 – 127HelicalSequence analysisAdd BLAST21
Topological domaini128 – 267LumenalSequence analysisAdd BLAST140
Transmembranei268 – 288HelicalSequence analysisAdd BLAST21
Topological domaini289 – 620CytoplasmicBy similarityAdd BLAST332

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001932051 – 620Very long-chain acyl-CoA synthetaseAdd BLAST620

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei291N6-acetyllysineCombined sources1
Modified residuei577PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiO35488
PaxDbiO35488
PeptideAtlasiO35488
PRIDEiO35488

PTM databases

iPTMnetiO35488
PhosphoSitePlusiO35488
SwissPalmiO35488

Expressioni

Tissue specificityi

Strong expression in liver and kidney, low expression in brain and testis, no expression in skeletal muscle and spleen. Shows uniform distribution in liver acinus.1 Publication

Gene expression databases

BgeeiENSMUSG00000027359
ExpressionAtlasiO35488 baseline and differential
GenevisibleiO35488 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

IntActiO35488, 4 interactors
MINTiO35488
STRINGi10090.ENSMUSP00000057595

Structurei

3D structure databases

ProteinModelPortaliO35488
SMRiO35488
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1179 Eukaryota
ENOG410XQ8T LUCA
GeneTreeiENSGT00550000074420
HOGENOMiHOG000044189
HOVERGENiHBG005642
InParanoidiO35488
KOiK08746
OMAiVGLHGCI
OrthoDBiEOG091G0B76
TreeFamiTF313430

Family and domain databases

InterProiView protein in InterPro
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
IPR030305 FATP2
PANTHERiPTHR43107:SF13 PTHR43107:SF13, 1 hit
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

O35488-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPVLYTGLA GLLLLPLLLT CCCPYLLQDV RYFLRLANMA RRVRSYRQRR
60 70 80 90 100
PVRTILRAFL EQARKTPHKP FLLFRDETLT YAQVDRRSNQ VARALHDQLG
110 120 130 140 150
LRQGDCVALF MGNEPAYVWI WLGLLKLGCP MACLNYNIRA KSLLHCFQCC
160 170 180 190 200
GAKVLLASPD LQEAVEEVLP TLKKDAVSVF YVSRTSNTNG VDTILDKVDG
210 220 230 240 250
VSAEPTPESW RSEVTFTTPA VYIYTSGTTG LPKAATINHH RLWYGTGLAM
260 270 280 290 300
SSGITAQDVI YTTMPLYHSA ALMIGLHGCI VVGATLALRS KFSASQFWDD
310 320 330 340 350
CRKYNVTVIQ YIGELLRYLC NTPQKPNDRD HKVKKALGNG LRGDVWREFI
360 370 380 390 400
KRFGDIHVYE FYASTEGNIG FVNYPRKIGA VGRANYLQRK VARYELIKYD
410 420 430 440 450
VEKDEPVRDA NGYCIKVPKG EVGLLVCKIT QLTPFIGYAG GKTQTEKKKL
460 470 480 490 500
RDVFKKGDIY FNSGDLLMID RENFVYFHDR VGDTFRWKGE NVATTEVADI
510 520 530 540 550
VGLVDFVEEV NVYGVPVPGH EGRIGMASLK IKENYEFNGK KLFQHIAEYL
560 570 580 590 600
PSYARPRFLR IQDTIEITGT FKHRKVTLME EGFNPTVIKD TLYFMDDAEK
610 620
TFVPMTENIY NAIIDKTLKL
Length:620
Mass (Da):70,423
Last modified:July 27, 2011 - v2
Checksum:i62994BDB1D828B37
GO

Sequence cautioni

The sequence AAC40186 differs from that shown. Reason: Frameshift at positions 253, 263, 267, 281, 289, 293, 299, 301 and 307.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35R → Q in AAB87982 (PubMed:11980911).Curated1
Sequence conflicti168V → A in CAA11687 (PubMed:9559670).Curated1
Sequence conflicti168V → A in AAB87982 (PubMed:11980911).Curated1
Sequence conflicti234 – 235AA → SG in AAC40186 (PubMed:9671728).Curated2
Sequence conflicti243W → R in AAC40186 (PubMed:9671728).Curated1
Sequence conflicti247G → S in AAC40186 (PubMed:9671728).Curated1
Sequence conflicti257Q → K in AAC40186 (PubMed:9671728).Curated1
Sequence conflicti271A → T in AAC40186 (PubMed:9671728).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ223958 mRNA Translation: CAA11687.1
AF072757 mRNA Translation: AAC40186.1 Sequence problems.
AF033031 mRNA Translation: AAB87982.1
AL831764, AL844555 Genomic DNA Translation: CAM17405.1
AL844555, AL831764 Genomic DNA Translation: CAM18704.1
BC013442 mRNA Translation: AAH13442.1
BC022170 mRNA Translation: AAH22170.1
BC024735 mRNA Translation: AAH24735.1
CCDSiCCDS16683.1
RefSeqiNP_036108.2, NM_011978.2
UniGeneiMm.290044

Genome annotation databases

EnsembliENSMUST00000061491; ENSMUSP00000057595; ENSMUSG00000027359
GeneIDi26458
KEGGimmu:26458
UCSCiuc008mdq.1 mouse

Similar proteinsi

Entry informationi

Entry nameiS27A2_MOUSE
AccessioniPrimary (citable) accession number: O35488
Secondary accession number(s): O70550, O88560, Q91WV6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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