ID   3BHS6_MOUSE             Reviewed;         373 AA.
AC   O35469;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   16-JUN-2009, entry version 64.
DE   RecName: Full=3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 6;
DE   AltName: Full=3-beta-HSD VI;
DE   Includes:
DE     RecName: Full=3-beta-hydroxy-Delta(5)-steroid dehydrogenase;
DE              EC=1.1.1.145;
DE     AltName: Full=3-beta-hydroxy-5-ene steroid dehydrogenase;
DE     AltName: Full=Progesterone reductase;
DE   Includes:
DE     RecName: Full=Steroid Delta-isomerase;
DE              EC=5.3.3.1;
DE     AltName: Full=Delta-5-3-ketosteroid isomerase;
GN   Name=Hsd3b6;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=C57BL/6J;
RX   MEDLINE=97230115; PubMed=9075693; DOI=10.1210/en.138.4.1392;
RA   Abbaszade I.G., Arensburg J., Park C.-H.J., Kasa-Vubu J.Z., Orly J.,
RA   Payne A.H.;
RT   "Isolation of a new mouse 3beta-hydroxysteroid dehydrogenase isoform,
RT   3beta-HSD VI, expressed during early pregnancy.";
RL   Endocrinology 138:1392-1399(1997).
CC   -!- FUNCTION: 3-beta-HSD is a bifunctional enzyme, that catalyzes the
CC       oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and
CC       the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic
CC       system plays a crucial role in the biosynthesis of all classes of
CC       hormonal steroids. May be involved in local production of
CC       progesterone.
CC   -!- CATALYTIC ACTIVITY: A 3-beta-hydroxy-Delta(5)-steroid + NAD(+) = a
CC       3-oxo-Delta(5)-steroid + NADH.
CC   -!- CATALYTIC ACTIVITY: A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-
CC       steroid.
CC   -!- PATHWAY: Lipid metabolism; steroid biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass
CC       membrane protein. Mitochondrion membrane; Single-pass membrane
CC       protein.
CC   -!- TISSUE SPECIFICITY: Expressed in the skin and also is expressed in
CC       the testis.
CC   -!- DEVELOPMENTAL STAGE: Earliest isoform to be expressed during
CC       embryogenesis in cells of embryonic origin at 7 and 9.5 dpc, and
CC       is the major isoform expressed in uterine tissue at the time of
CC       implantation (4.5 dpc) and continues to be expressed in uterine
CC       tissue at 6.5, 7.5 and 9.5 dpc. It is expressed in giant
CC       trophoblasts at 9.5 dpc and is expressed in the placenta through
CC       15.5 dpc.
CC   -!- SIMILARITY: Belongs to the 3-beta-HSD family.
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DR   EMBL; AF031170; AAB84299.1; -; mRNA.
DR   IPI; IPI00420666; -.
DR   UniGene; Mm.14435; -.
DR   Ensembl; ENSMUSG00000027869; Mus musculus.
DR   MGI; MGI:109598; Hsd3b6.
DR   HOGENOM; O35469; -.
DR   HOVERGEN; O35469; -.
DR   BRENDA; 1.1.1.145; 244.
DR   BRENDA; 5.3.3.1; 244.
DR   Bgee; O35469; -.
DR   GermOnline; ENSMUSG00000027869; Mus musculus.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0003854; F:3-beta-hydroxy-delta5-steroid dehydrogenase...; IEA:EC.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0004769; F:steroid delta-isomerase activity; IEA:EC.
DR   GO; GO:0006700; P:C21-steroid hormone biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR002225; 3Beta_OHSteriod_DH/Estase.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF01073; 3Beta_HSD; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Isomerase; Membrane; Mitochondrion;
KW   Multifunctional enzyme; NAD; Oxidoreductase; Steroidogenesis;
KW   Transmembrane.
FT   INIT_MET      1      1       Removed (By similarity).
FT   CHAIN         2    373       3 beta-hydroxysteroid dehydrogenase/Delta
FT                                5-->4-isomerase type 6.
FT                                /FTId=PRO_0000087785.
FT   TRANSMEM    288    308       Potential.
FT   ACT_SITE    155    155       Proton acceptor (By similarity).
FT   BINDING     159    159       NAD (By similarity).
SQ   SEQUENCE   373 AA;  41876 MW;  A9718D6A5832C52D CRC64;
     MPGWSCLVTG AGGFLGQRIV QLLMQEKDLE EIRVLDKFFR PETREQFFNL DTNIKVTVLE
     GDILDTQYLR KACQGISVVI HTAAVIDVTG VIPRQTILDV NLKGTQNLLE ACIQASVPAF
     IFSSSVDVAG PNSYKEIILN GNEEEHHESI WSDPYPYSKK MAEKAVLAAN GSMLKIGGTL
     HTCALRPMFI YGERSPFISN TIITALKNKN ILGCTGKFST ANPVYVGNVA WAHILAARGL
     RDPKKSPNIQ GEFYYISDDT PHQSYDDLNY TLSKEWGFCP DSSWSLPVPL LYWLAFMLET
     VSFLLSPIYR FIPPFNRHLV TLTGSTFTFS YKKAQRDLGY EPLVSWEEAK QKTSEWIGTL
     VEQHRATLDT TSQ
//