Reviewed,
UniProtKB/Swiss-Prot O35469 (3BHS6_MOUSE)
Last modified
February 9, 2010.
Version 71.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 6 EC=1.1.1.- Alternative name(s): 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type VI Short name=3-beta-HSD VI Including the following 2 domains: 1- Recommended name: 3-beta-hydroxy-Delta(5)-steroid dehydrogenase EC=1.1.1.145 Alternative name(s): 3-beta-hydroxy-5-ene steroid dehydrogenase Progesterone reductase 2- Recommended name: Steroid Delta-isomerase EC=5.3.3.1 Alternative name(s): Delta-5-3-ketosteroid isomerase | ||
| Gene names |
| ||
| Organism | Mus musculus (Mouse) | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 373 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. May be involved in local production of progesterone. |
| Catalytic activity | A 3-beta-hydroxy-Delta(5)-steroid + NAD+ = a 3-oxo-Delta(5)-steroid + NADH. A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid. |
| Pathway | |
| Subcellular location | Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein. |
| Tissue specificity | Expressed in the skin and also is expressed in the testis. |
| Developmental stage | Earliest isoform to be expressed during embryogenesis in cells of embryonic origin at 7 and 9.5 dpc, and is the major isoform expressed in uterine tissue at the time of implantation (4.5 dpc) and continues to be expressed in uterine tissue at 6.5, 7.5 and 9.5 dpc. It is expressed in giant trophoblasts at 9.5 dpc and is expressed in the placenta through 15.5 dpc. |
| Sequence similarities | Belongs to the 3-beta-HSD family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Steroidogenesis |
| Cellular component | Endoplasmic reticulum Membrane Mitochondrion |
| Domain | Transmembrane |
| Ligand | NAD |
| Molecular function | Isomerase Oxidoreductase |
| Technical term | Multifunctional enzyme |
| Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic processInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membraneInferred from electronic annotation. Source: UniProtKB-SubCell mitochondrionInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | 3-beta-hydroxy-delta5-steroid dehydrogenase activity Inferred from electronic annotation. Source: EC bindingInferred from electronic annotation. Source: InterPro steroid delta-isomerase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed By similarity | ||||||
| Chain | 2 – 373 | 372 | 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 6 | PRO_0000087785 | |||||
Regions | |||||||||
| Transmembrane | 288 – 308 | 21 | Potential | ||||||
Sites | |||||||||
| Active site | 155 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 159 | 1 | NAD By similarity | ||||||
Sequences
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References
| [1] | "Isolation of a new mouse 3beta-hydroxysteroid dehydrogenase isoform, 3beta-HSD VI, expressed during early pregnancy." Abbaszade I.G., Arensburg J., Park C.-H.J., Kasa-Vubu J.Z., Orly J., Payne A.H. Endocrinology 138:1392-1399(1997) [PubMed: 9075693] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF031170 mRNA. Translation: AAB84299.1. |
| IPI | IPI00420666. |
| UniGene | Mm.14435 |
3D structure databases | |
| SMR | O35469. Positions 5-364. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | O35469. |
Proteomic databases | |
| PRIDE | O35469. |
Genome annotation databases | |
| Ensembl | ENSMUST00000029463; ENSMUSP00000029463; ENSMUSG00000027869; Mus musculus. [Genome view] |
| UCSC | uc008qqg.1. mouse. |
Organism-specific databases | |
| MGI | MGI:109598. Hsd3b6. |
Phylogenomic databases | |
| eggNOG | roNOG12122. |
| HOGENOM | HBG755066. |
| HOVERGEN | O35469. |
| InParanoid | O35469. |
| PhylomeDB | O35469. |
Enzyme and pathway databases | |
| BRENDA | 1.1.1.145. 244. 5.3.3.1. 244. |
Gene expression databases | |
| Bgee | O35469. |
| Genevestigator | O35469. |
| GermOnline | ENSMUSG00000027869. Mus musculus. |
Family and domain databases | |
| InterPro | IPR016040. NAD(P)-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| ProtoNet | Search... |
Other Resources | |
| SOURCE | Search... |
Entry information
| Entry name | 3BHS6_MOUSE | ||||||||
| Accession | Primary (citable) accession number: O35469 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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