O35469 (3BHS6_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 89.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 6 EC=1.1.1.- Alternative name(s): 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type VI Short name=3-beta-HSD VI | ||
| Gene names |
| ||
| Organism | Mus musculus (Mouse) | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus |
Protein attributes
| Sequence length | 373 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. May be involved in local production of progesterone. |
| Catalytic activity | A 3-beta-hydroxy-Delta(5)-steroid + NAD+ = a 3-oxo-Delta(5)-steroid + NADH. A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid. |
| Pathway | |
| Subcellular location | Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein. |
| Tissue specificity | Expressed in the skin and also is expressed in the testis. |
| Developmental stage | Earliest isoform to be expressed during embryogenesis in cells of embryonic origin at 7 and 9.5 dpc, and is the major isoform expressed in uterine tissue at the time of implantation (4.5 dpc) and continues to be expressed in uterine tissue at 6.5, 7.5 and 9.5 dpc. It is expressed in giant trophoblasts at 9.5 dpc and is expressed in the placenta through 15.5 dpc. |
| Sequence similarities | Belongs to the 3-beta-HSD family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Steroidogenesis |
| Cellular component | Endoplasmic reticulum Membrane Mitochondrion |
| Domain | Transmembrane Transmembrane helix |
| Ligand | NAD |
| Molecular function | Isomerase Oxidoreductase |
| Technical term | Complete proteome Multifunctional enzyme Reference proteome |
| Gene Ontology (GO) | |
| Biological process | steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membraneInferred from electronic annotation. Source: UniProtKB-KW mitochondrial membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | 3-beta-hydroxy-delta5-steroid dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide bindingInferred from electronic annotation. Source: InterPro steroid delta-isomerase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed By similarity | ||||||
| Chain | 2 – 373 | 372 | 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 6 | PRO_0000087785 | |||||
Regions | |||||||||
| Transmembrane | 288 – 308 | 21 | Helical; Potential | ||||||
Sites | |||||||||
| Active site | 155 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 159 | 1 | NAD By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 189 | 1 | Y → F in AAB84299. Ref.1 | ||||||
| Sequence conflict | 366 | 1 | E → A in AAB84299. Ref.1 | ||||||
| Sequence conflict | 371 | 1 | K → T in AAB84299. Ref.1 | ||||||
Sequences
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References
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF031170 mRNA. Translation: AAB84299.1. AK142267 mRNA. Translation: BAE25002.1. AL606755 Genomic DNA. Translation: CAM13693.1. CH466620 Genomic DNA. Translation: EDL38971.1. |
| IPI | IPI00420666. |
| RefSeq | NP_038849.2. NM_013821.3. |
| UniGene | Mm.14435. |
3D structure databases | |
| ProteinModelPortal | O35469. |
| SMR | O35469. Positions 3-360. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | O35469. |
Proteomic databases | |
| PRIDE | O35469. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSMUST00000029463; ENSMUSP00000029463; ENSMUSG00000027869. ENSMUST00000170847; ENSMUSP00000129911; ENSMUSG00000027869. |
| GeneID | 15497. |
| KEGG | mmu:15497. |
| NMPDR | fig|10090.3.peg.8657. |
Organism-specific databases | |
| CTD | 15497. |
| MGI | MGI:109598. Hsd3b6. |
Phylogenomic databases | |
| eggNOG | roNOG12122. |
| HOGENOM | HBG755066. |
| HOVERGEN | HBG000014. |
| InParanoid | O35469. |
| OrthoDB | EOG4K3KWG. |
| PhylomeDB | O35469. |
Enzyme and pathway databases | |
| BRENDA | 1.1.1.145. 244. 5.3.3.1. 244. |
Gene expression databases | |
| Bgee | O35469. |
| Genevestigator | O35469. |
| GermOnline | ENSMUSG00000027869. Mus musculus. |
Family and domain databases | |
| InterPro | IPR002225. 3Beta_OHSteriod_DH/Estase. IPR016040. NAD(P)-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| KO | K00070. |
| Pfam | PF01073. 3Beta_HSD. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 288386. |
| SOURCE | Search... |
Entry information
| Entry name | 3BHS6_MOUSE | ||||||||
| Accession | Primary (citable) accession number: O35469 Secondary accession number(s): Q3UQN7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with