Reviewed, UniProtKB/Swiss-Prot O35469 (3BHS6_MOUSE)

Names and origin

Protein names

Recommended name:
    3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 6
    EC=1.1.1.-
Alternative name(s):
    3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type VI
      Short name=3-beta-HSD VI
Including the following 2 domains:
    1- Recommended name:
            3-beta-hydroxy-Delta(5)-steroid dehydrogenase
              EC=1.1.1.145
        Alternative name(s):
            3-beta-hydroxy-5-ene steroid dehydrogenase
            Progesterone reductase
    2- Recommended name:
            Steroid Delta-isomerase
              EC=5.3.3.1
        Alternative name(s):
            Delta-5-3-ketosteroid isomerase

Gene names

Name:

Hsd3b6

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. May be involved in local production of progesterone.
Catalytic activity	A 3-beta-hydroxy-Delta(5)-steroid + NAD+ = a 3-oxo-Delta(5)-steroid + NADH. A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.
Pathway	Lipid metabolism; steroid biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein.
Tissue specificity	Expressed in the skin and also is expressed in the testis.
Developmental stage	Earliest isoform to be expressed during embryogenesis in cells of embryonic origin at 7 and 9.5 dpc, and is the major isoform expressed in uterine tissue at the time of implantation (4.5 dpc) and continues to be expressed in uterine tissue at 6.5, 7.5 and 9.5 dpc. It is expressed in giant trophoblasts at 9.5 dpc and is expressed in the placenta through 15.5 dpc.
Sequence similarities	Belongs to the 3-beta-HSD family.

Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Endoplasmic reticulum Membrane Mitochondrion
Domain	Transmembrane
Ligand	NAD
Molecular function	Isomerase Oxidoreductase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-beta-hydroxy-delta5-steroid dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro steroid delta-isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 373	372	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 6		PRO_0000087785
Regions
	Transmembrane	288 – 308	21	Potential
Sites
	Active site	155	1	Proton acceptor By similarity
	Binding site	159	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	O35469-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: A9718D6A5832C52D	FASTA	373	41,876
	10 20 30 40 50 60 MPGWSCLVTG AGGFLGQRIV QLLMQEKDLE EIRVLDKFFR PETREQFFNL DTNIKVTVLE 70 80 90 100 110 120 GDILDTQYLR KACQGISVVI HTAAVIDVTG VIPRQTILDV NLKGTQNLLE ACIQASVPAF 130 140 150 160 170 180 IFSSSVDVAG PNSYKEIILN GNEEEHHESI WSDPYPYSKK MAEKAVLAAN GSMLKIGGTL 190 200 210 220 230 240 HTCALRPMFI YGERSPFISN TIITALKNKN ILGCTGKFST ANPVYVGNVA WAHILAARGL 250 260 270 280 290 300 RDPKKSPNIQ GEFYYISDDT PHQSYDDLNY TLSKEWGFCP DSSWSLPVPL LYWLAFMLET 310 320 330 340 350 360 VSFLLSPIYR FIPPFNRHLV TLTGSTFTFS YKKAQRDLGY EPLVSWEEAK QKTSEWIGTL 370 VEQHRATLDT TSQ

References

[1]	"Isolation of a new mouse 3beta-hydroxysteroid dehydrogenase isoform, 3beta-HSD VI, expressed during early pregnancy." Abbaszade I.G., Arensburg J., Park C.-H.J., Kasa-Vubu J.Z., Orly J., Payne A.H. Endocrinology 138:1392-1399(1997) [PubMed: 9075693] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF031170 mRNA. Translation: AAB84299.1.
IPI	IPI00420666.
UniGene	Mm.14435
3D structure databases
SMR	O35469. Positions 5-364.
ModBase	Search...
Protein-protein interaction databases
STRING	O35469.
Proteomic databases
PRIDE	O35469.
Genome annotation databases
Ensembl	ENSMUST00000029463; ENSMUSP00000029463; ENSMUSG00000027869; Mus musculus. [Genome view]
UCSC	uc008qqg.1. mouse.
Organism-specific databases
MGI	MGI:109598. Hsd3b6.
Phylogenomic databases
eggNOG	roNOG12122.
HOGENOM	HBG755066.
HOVERGEN	O35469.
InParanoid	O35469.
PhylomeDB	O35469.
Enzyme and pathway databases
BRENDA	1.1.1.145. 244. 5.3.3.1. 244.
Gene expression databases
Bgee	O35469.
Genevestigator	O35469.
GermOnline	ENSMUSG00000027869. Mus musculus.
Family and domain databases
InterPro	IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

3BHS6_MOUSE

Accession

Primary (citable) accession number: O35469

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 71 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)