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Protein

Angiopoietin-2

Gene

Angpt2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi429CalciumBy similarity1
Metal bindingi431CalciumBy similarity1
Metal bindingi433Calcium; via carbonyl oxygenBy similarity1
Metal bindingi435Calcium; via carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

  • angiogenesis Source: RGD
  • animal organ regeneration Source: RGD
  • cellular response to growth factor stimulus Source: RGD
  • germ cell development Source: RGD
  • glomerulus vasculature development Source: UniProtKB
  • maternal process involved in female pregnancy Source: RGD
  • negative regulation of angiogenesis Source: Ensembl
  • negative regulation of blood vessel endothelial cell migration Source: Ensembl
  • negative regulation of cell-substrate adhesion Source: Ensembl
  • negative regulation of positive chemotaxis Source: Ensembl
  • positive regulation of angiogenesis Source: RGD
  • response to activity Source: RGD
  • response to glucose Source: RGD
  • response to hypoxia Source: RGD
  • response to mechanical stimulus Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to radiation Source: RGD
  • Tie signaling pathway Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Angiogenesis, Differentiation

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-210993. Tie2 Signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Angiopoietin-2
Short name:
ANG-2
Gene namesi
Name:Angpt2
Synonyms:Agpt2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 16

Organism-specific databases

RGDi621861. Angpt2.

Subcellular locationi

GO - Cellular componenti

  • cell projection Source: RGD
  • extracellular space Source: RGD
  • nucleus Source: RGD
  • plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000009906419 – 496Angiopoietin-2Add BLAST478

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi89N-linked (GlcNAc...)Sequence analysis1
Glycosylationi119N-linked (GlcNAc...)Sequence analysis1
Glycosylationi133N-linked (GlcNAc...)Sequence analysis1
Glycosylationi151N-linked (GlcNAc...)Sequence analysis1
Glycosylationi240N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi284 ↔ 313PROSITE-ProRule annotation
Glycosylationi304N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi433 ↔ 435PROSITE-ProRule annotation
Disulfide bondi437 ↔ 450PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO35462.
PRIDEiO35462.

PTM databases

iPTMnetiO35462.
PhosphoSitePlusiO35462.

Expressioni

Gene expression databases

BgeeiENSRNOG00000016696.
GenevisibleiO35462. RN.

Interactioni

Subunit structurei

Interacts with TEK/TIE2, competing for the same binding site as ANGPT1.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022774.

Structurei

3D structure databases

ProteinModelPortaliO35462.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini280 – 496Fibrinogen C-terminalPROSITE-ProRule annotationAdd BLAST217

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili159 – 256Sequence analysisAdd BLAST98

Domaini

The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor.By similarity

Sequence similaritiesi

Contains 1 fibrinogen C-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Signal

Phylogenomic databases

eggNOGiKOG2579. Eukaryota.
ENOG410ZYS4. LUCA.
GeneTreeiENSGT00760000118809.
HOGENOMiHOG000037128.
HOVERGENiHBG001644.
InParanoidiO35462.
KOiK05466.
OMAiTWKEYKV.
OrthoDBiEOG091G03M1.
PhylomeDBiO35462.
TreeFamiTF336658.

Family and domain databases

CDDicd00087. FReD. 1 hit.
Gene3Di3.90.215.10. 1 hit.
4.10.530.10. 1 hit.
InterProiIPR028844. Ang-2.
IPR014716. Fibrinogen_a/b/g_C_1.
IPR014715. Fibrinogen_a/b/g_C_2.
IPR002181. Fibrinogen_a/b/g_C_dom.
IPR020837. Fibrinogen_CS.
[Graphical view]
PANTHERiPTHR19143:SF199. PTHR19143:SF199. 1 hit.
PfamiPF00147. Fibrinogen_C. 1 hit.
[Graphical view]
SMARTiSM00186. FBG. 1 hit.
[Graphical view]
SUPFAMiSSF56496. SSF56496. 1 hit.
PROSITEiPS00514. FIBRINOGEN_C_1. 1 hit.
PS51406. FIBRINOGEN_C_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35462-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWQIVFLTFG CDLVLASAYN NFRKSVDSTG RRQYQVQNGP CSYTFLLPET
60 70 80 90 100
DSCRSSSSPY MSNAVQRDAP LDYDDSVQRL QVLENILENN TQWLMKLENY
110 120 130 140 150
IQDNMKKEMV EIQQNVVQNQ TAVMIEIGTS LLNQTAAQTR KLTDVEAQVL
160 170 180 190 200
NQTTRLELQL LQHSISTNKL EKQILDQTSE INKLQDKNSF LEKKVLDMED
210 220 230 240 250
KHSVQLQSMK EQKDQLQVLV SKQSSVIDEL EKKLVTATVN NSVLQKQQHD
260 270 280 290 300
LMETVNSLLT MMSSPDYKSS VAVPKEEKTT FRDCAEIFKS GLTTSGIYTL
310 320 330 340 350
TFPNSTEEVK AYCDMDMGGG GWTVIQHRED GSVDFQRTWK EYKEGFGSPL
360 370 380 390 400
GEYWLGNEFV SQLTSGHRYV LKIQLKDWEG SEAHSLYEHF YLSGEESNYR
410 420 430 440 450
IHLTGLTGTA GKISSISQPG SDFSTKDSDN DKCICKCSQM LTGGWWFDAC
460 470 480 490
GPSNLNGQYY PQKQNTNKFN GIKWYYWKGS GYSLKATTMM IRPADF
Length:496
Mass (Da):56,518
Last modified:March 24, 2009 - v2
Checksum:iC6F3532A89564C06
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473970 Genomic DNA. Translation: EDM08957.1.
BC161931 mRNA. Translation: AAI61931.1.
AF030378 mRNA. Translation: AAC78247.1.
AF311728 mRNA. Translation: AAG34114.1.
RefSeqiNP_604449.1. NM_134454.1.
UniGeneiRn.138360.

Genome annotation databases

EnsembliENSRNOT00000022774; ENSRNOP00000022774; ENSRNOG00000016696.
GeneIDi89805.
KEGGirno:89805.
UCSCiRGD:621861. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473970 Genomic DNA. Translation: EDM08957.1.
BC161931 mRNA. Translation: AAI61931.1.
AF030378 mRNA. Translation: AAC78247.1.
AF311728 mRNA. Translation: AAG34114.1.
RefSeqiNP_604449.1. NM_134454.1.
UniGeneiRn.138360.

3D structure databases

ProteinModelPortaliO35462.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022774.

PTM databases

iPTMnetiO35462.
PhosphoSitePlusiO35462.

Proteomic databases

PaxDbiO35462.
PRIDEiO35462.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022774; ENSRNOP00000022774; ENSRNOG00000016696.
GeneIDi89805.
KEGGirno:89805.
UCSCiRGD:621861. rat.

Organism-specific databases

CTDi285.
RGDi621861. Angpt2.

Phylogenomic databases

eggNOGiKOG2579. Eukaryota.
ENOG410ZYS4. LUCA.
GeneTreeiENSGT00760000118809.
HOGENOMiHOG000037128.
HOVERGENiHBG001644.
InParanoidiO35462.
KOiK05466.
OMAiTWKEYKV.
OrthoDBiEOG091G03M1.
PhylomeDBiO35462.
TreeFamiTF336658.

Enzyme and pathway databases

ReactomeiR-RNO-210993. Tie2 Signaling.

Miscellaneous databases

PROiO35462.

Gene expression databases

BgeeiENSRNOG00000016696.
GenevisibleiO35462. RN.

Family and domain databases

CDDicd00087. FReD. 1 hit.
Gene3Di3.90.215.10. 1 hit.
4.10.530.10. 1 hit.
InterProiIPR028844. Ang-2.
IPR014716. Fibrinogen_a/b/g_C_1.
IPR014715. Fibrinogen_a/b/g_C_2.
IPR002181. Fibrinogen_a/b/g_C_dom.
IPR020837. Fibrinogen_CS.
[Graphical view]
PANTHERiPTHR19143:SF199. PTHR19143:SF199. 1 hit.
PfamiPF00147. Fibrinogen_C. 1 hit.
[Graphical view]
SMARTiSM00186. FBG. 1 hit.
[Graphical view]
SUPFAMiSSF56496. SSF56496. 1 hit.
PROSITEiPS00514. FIBRINOGEN_C_1. 1 hit.
PS51406. FIBRINOGEN_C_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiANGP2_RAT
AccessioniPrimary (citable) accession number: O35462
Secondary accession number(s): B1WBY0, Q548N5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: March 24, 2009
Last modified: November 30, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.