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O35459 (ECH1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial

EC=5.3.3.-
Gene names
Name:Ech1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length327 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA By similarity.

Pathway

Lipid metabolism; fatty acid beta-oxidation.

Subunit structure

Homohexamer By similarity.

Subcellular location

Mitochondrion By similarity. Peroxisome By similarity.

Sequence similarities

Belongs to the enoyl-CoA hydratase/isomerase family.

Ontologies

Keywords
   Biological processFatty acid metabolism
Lipid metabolism
   Cellular componentMitochondrion
Peroxisome
   DomainTransit peptide
   Molecular functionIsomerase
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processfatty acid beta-oxidation

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentmitochondrion

Inferred from direct assay PubMed 14651853PubMed 18614015. Source: MGI

peroxisome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionisomerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3333Mitochondrion Potential
Chain34 – 327294Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
PRO_0000007418

Regions

Motif325 – 3273Microbody targeting signal Potential

Sites

Binding site1731Substrate; via amide nitrogen By similarity
Site1961Important for catalytic activity By similarity

Amino acid modifications

Modified residue1471N6-acetyllysine Ref.4
Modified residue2301N6-succinyllysine Ref.3
Modified residue3161N6-succinyllysine Ref.3
Modified residue3261N6-acetyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
O35459 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: CC0E612C74FBEE2E

FASTA32736,118
        10         20         30         40         50         60 
MATAMTVSSK LRGLLMQQLR GTSQLYFNIS LRSLSSSAQE ASKRAPEEVS DHNYESIQVT 

        70         80         90        100        110        120 
SAQKHVLHVQ LNRPEKRNAM NRAFWRELVE CFQKISKDSD CRAVVVSGAG KMFTSGIDLM 

       130        140        150        160        170        180 
DMASELMQPS GDDAARIAWY LRDLISKYQK TFTVIEKCPK PVIAAIHGGC IGGGVDLVSA 

       190        200        210        220        230        240 
CDIRYCTQDA FFQIKEVDMG LAADVGTLQR LPKVIGNQSL VNELTFSARK MMADEALDSG 

       250        260        270        280        290        300 
LVSRVFQDKD AMLNAAFALA ADISSKSPVA VQGSKINLIY SRDHSVDESL DYMATWNMSM 

       310        320 
LQTQDIIKSV QAAMEKRDTK SITFSKL 

« Hide

References

« Hide 'large scale' references
[1]"Identification and characterisation of mouse ECH1."
Fitzpatrick D.R.
Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain and Testis.
[3]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-230 AND LYS-316, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[4]"Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-147, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF030343 mRNA. Translation: AAB84224.1.
BC068112 mRNA. Translation: AAH68112.1.
BC087924 mRNA. Translation: AAH87924.1.
RefSeqNP_058052.1. NM_016772.1.
UniGeneMm.291776.

3D structure databases

ProteinModelPortalO35459.
SMRO35459. Positions 54-327.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO35459. 4 interactions.
MINTMINT-1859888.
STRING10090.ENSMUSP00000066092.

PTM databases

PhosphoSiteO35459.

2D gel databases

REPRODUCTION-2DPAGEO35459.

Proteomic databases

PaxDbO35459.
PRIDEO35459.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000066264; ENSMUSP00000066092; ENSMUSG00000053898.
GeneID51798.
KEGGmmu:51798.
UCSCuc009gab.1. mouse.

Organism-specific databases

CTD1891.
MGIMGI:1858208. Ech1.

Phylogenomic databases

eggNOGCOG1024.
GeneTreeENSGT00720000108577.
HOGENOMHOG000027939.
HOVERGENHBG005556.
InParanoidO35459.
KOK12663.
OMAEIDMGMA.
OrthoDBEOG7JHM64.
PhylomeDBO35459.
TreeFamTF314317.

Enzyme and pathway databases

UniPathwayUPA00659.

Gene expression databases

ArrayExpressO35459.
BgeeO35459.
CleanExMM_ECH1.
GenevestigatorO35459.

Family and domain databases

Gene3D1.10.12.10. 1 hit.
InterProIPR014748. Crontonase_C.
IPR001753. Crotonase_core_superfam.
IPR018376. Enoyl-CoA_hyd/isom_CS.
[Graphical view]
PfamPF00378. ECH. 1 hit.
[Graphical view]
PROSITEPS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio308044.
PROO35459.
SOURCESearch...

Entry information

Entry nameECH1_MOUSE
AccessionPrimary (citable) accession number: O35459
Secondary accession number(s): Q5M8P6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot