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O35459

- ECH1_MOUSE

UniProt

O35459 - ECH1_MOUSE

Protein

Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial

Gene

Ech1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei173 – 1731Substrate; via amide nitrogenBy similarity
    Sitei196 – 1961Important for catalytic activityBy similarity

    GO - Molecular functioni

    1. isomerase activity Source: UniProtKB-KW

    GO - Biological processi

    1. fatty acid beta-oxidation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Enzyme and pathway databases

    UniPathwayiUPA00659.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial (EC:5.3.3.-)
    Gene namesi
    Name:Ech1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1858208. Ech1.

    Subcellular locationi

    Mitochondrion By similarity. Peroxisome By similarity

    GO - Cellular componenti

    1. mitochondrion Source: MGI
    2. peroxisome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion, Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3333MitochondrionSequence AnalysisAdd
    BLAST
    Chaini34 – 327294Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrialPRO_0000007418Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei147 – 1471N6-acetyllysine1 Publication
    Modified residuei230 – 2301N6-succinyllysine1 Publication
    Modified residuei316 – 3161N6-succinyllysine1 Publication
    Modified residuei326 – 3261N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiO35459.
    PaxDbiO35459.
    PRIDEiO35459.

    2D gel databases

    REPRODUCTION-2DPAGEO35459.

    PTM databases

    PhosphoSiteiO35459.

    Expressioni

    Gene expression databases

    ArrayExpressiO35459.
    BgeeiO35459.
    CleanExiMM_ECH1.
    GenevestigatoriO35459.

    Interactioni

    Subunit structurei

    Homohexamer.By similarity

    Protein-protein interaction databases

    IntActiO35459. 4 interactions.
    MINTiMINT-1859888.
    STRINGi10090.ENSMUSP00000066092.

    Structurei

    3D structure databases

    ProteinModelPortaliO35459.
    SMRiO35459. Positions 54-327.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi325 – 3273Microbody targeting signalSequence Analysis

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1024.
    GeneTreeiENSGT00720000108577.
    HOGENOMiHOG000027939.
    HOVERGENiHBG005556.
    InParanoidiO35459.
    KOiK12663.
    OMAiSWVKDVC.
    OrthoDBiEOG7JHM64.
    PhylomeDBiO35459.
    TreeFamiTF314317.

    Family and domain databases

    Gene3Di1.10.12.10. 1 hit.
    3.90.226.10. 1 hit.
    InterProiIPR029045. ClpP/crotonase-like_dom.
    IPR014748. Crontonase_C.
    IPR001753. Crotonase_core_superfam.
    IPR018376. Enoyl-CoA_hyd/isom_CS.
    [Graphical view]
    PfamiPF00378. ECH. 1 hit.
    [Graphical view]
    SUPFAMiSSF52096. SSF52096. 1 hit.
    PROSITEiPS00166. ENOYL_COA_HYDRATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O35459-1 [UniParc]FASTAAdd to Basket

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    MATAMTVSSK LRGLLMQQLR GTSQLYFNIS LRSLSSSAQE ASKRAPEEVS    50
    DHNYESIQVT SAQKHVLHVQ LNRPEKRNAM NRAFWRELVE CFQKISKDSD 100
    CRAVVVSGAG KMFTSGIDLM DMASELMQPS GDDAARIAWY LRDLISKYQK 150
    TFTVIEKCPK PVIAAIHGGC IGGGVDLVSA CDIRYCTQDA FFQIKEVDMG 200
    LAADVGTLQR LPKVIGNQSL VNELTFSARK MMADEALDSG LVSRVFQDKD 250
    AMLNAAFALA ADISSKSPVA VQGSKINLIY SRDHSVDESL DYMATWNMSM 300
    LQTQDIIKSV QAAMEKRDTK SITFSKL 327
    Length:327
    Mass (Da):36,118
    Last modified:January 1, 1998 - v1
    Checksum:iCC0E612C74FBEE2E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF030343 mRNA. Translation: AAB84224.1.
    BC068112 mRNA. Translation: AAH68112.1.
    BC087924 mRNA. Translation: AAH87924.1.
    CCDSiCCDS21057.1.
    RefSeqiNP_058052.1. NM_016772.1.
    UniGeneiMm.291776.

    Genome annotation databases

    EnsembliENSMUST00000066264; ENSMUSP00000066092; ENSMUSG00000053898.
    GeneIDi51798.
    KEGGimmu:51798.
    UCSCiuc009gab.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF030343 mRNA. Translation: AAB84224.1 .
    BC068112 mRNA. Translation: AAH68112.1 .
    BC087924 mRNA. Translation: AAH87924.1 .
    CCDSi CCDS21057.1.
    RefSeqi NP_058052.1. NM_016772.1.
    UniGenei Mm.291776.

    3D structure databases

    ProteinModelPortali O35459.
    SMRi O35459. Positions 54-327.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O35459. 4 interactions.
    MINTi MINT-1859888.
    STRINGi 10090.ENSMUSP00000066092.

    PTM databases

    PhosphoSitei O35459.

    2D gel databases

    REPRODUCTION-2DPAGE O35459.

    Proteomic databases

    MaxQBi O35459.
    PaxDbi O35459.
    PRIDEi O35459.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000066264 ; ENSMUSP00000066092 ; ENSMUSG00000053898 .
    GeneIDi 51798.
    KEGGi mmu:51798.
    UCSCi uc009gab.1. mouse.

    Organism-specific databases

    CTDi 1891.
    MGIi MGI:1858208. Ech1.

    Phylogenomic databases

    eggNOGi COG1024.
    GeneTreei ENSGT00720000108577.
    HOGENOMi HOG000027939.
    HOVERGENi HBG005556.
    InParanoidi O35459.
    KOi K12663.
    OMAi SWVKDVC.
    OrthoDBi EOG7JHM64.
    PhylomeDBi O35459.
    TreeFami TF314317.

    Enzyme and pathway databases

    UniPathwayi UPA00659 .

    Miscellaneous databases

    NextBioi 308044.
    PROi O35459.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O35459.
    Bgeei O35459.
    CleanExi MM_ECH1.
    Genevestigatori O35459.

    Family and domain databases

    Gene3Di 1.10.12.10. 1 hit.
    3.90.226.10. 1 hit.
    InterProi IPR029045. ClpP/crotonase-like_dom.
    IPR014748. Crontonase_C.
    IPR001753. Crotonase_core_superfam.
    IPR018376. Enoyl-CoA_hyd/isom_CS.
    [Graphical view ]
    Pfami PF00378. ECH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52096. SSF52096. 1 hit.
    PROSITEi PS00166. ENOYL_COA_HYDRATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterisation of mouse ECH1."
      Fitzpatrick D.R.
      Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain and Testis.
    3. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-230 AND LYS-316, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    4. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
      Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
      Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-147, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiECH1_MOUSE
    AccessioniPrimary (citable) accession number: O35459
    Secondary accession number(s): Q5M8P6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3