O35459 (ECH1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
EC=5.3.3.-

Gene names

Name:

Ech1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA By similarity.
Pathway	Lipid metabolism; fatty acid beta-oxidation.
Subunit structure	Homohexamer By similarity.
Subcellular location	Mitochondrion By similarity. Peroxisome By similarity.
Sequence similarities	Belongs to the enoyl-CoA hydratase/isomerase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Mitochondrion Peroxisome
Domain	Transit peptide
Molecular function	Isomerase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fatty acid beta-oxidation Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	mitochondrion Inferred from direct assay PubMed 14651853PubMed 18614015. Source: MGI peroxisome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	isomerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 33	33	Mitochondrion Potential
	Chain	34 – 327	294	Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial		PRO_0000007418
Regions
	Motif	325 – 327	3	Microbody targeting signal Potential
Sites
	Binding site	173	1	Substrate; via amide nitrogen By similarity
	Site	196	1	Important for catalytic activity By similarity
Amino acid modifications
	Modified residue	94	1	N6-acetyllysine By similarity
	Modified residue	326	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O35459 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: CC0E612C74FBEE2E	FASTA	327	36,118
	10 20 30 40 50 60 MATAMTVSSK LRGLLMQQLR GTSQLYFNIS LRSLSSSAQE ASKRAPEEVS DHNYESIQVT 70 80 90 100 110 120 SAQKHVLHVQ LNRPEKRNAM NRAFWRELVE CFQKISKDSD CRAVVVSGAG KMFTSGIDLM 130 140 150 160 170 180 DMASELMQPS GDDAARIAWY LRDLISKYQK TFTVIEKCPK PVIAAIHGGC IGGGVDLVSA 190 200 210 220 230 240 CDIRYCTQDA FFQIKEVDMG LAADVGTLQR LPKVIGNQSL VNELTFSARK MMADEALDSG 250 260 270 280 290 300 LVSRVFQDKD AMLNAAFALA ADISSKSPVA VQGSKINLIY SRDHSVDESL DYMATWNMSM 310 320 LQTQDIIKSV QAAMEKRDTK SITFSKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterisation of mouse ECH1." Fitzpatrick D.R. Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain and Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF030343 mRNA. Translation: AAB84224.1. BC068112 mRNA. Translation: AAH68112.1. BC087924 mRNA. Translation: AAH87924.1.
IPI	IPI00130804.
RefSeq	NP_058052.1. NM_016772.1.
UniGene	Mm.291776.
3D structure databases
ProteinModelPortal	O35459.
SMR	O35459. Positions 54-327.
ModBase	Search...
Protein-protein interaction databases
IntAct	O35459. 1 interaction.
STRING	10090.ENSMUSP00000066092.
PTM databases
PhosphoSite	O35459.
2D gel databases
REPRODUCTION-2DPAGE	O35459.
Proteomic databases
PaxDb	O35459.
PRIDE	O35459.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000066264; ENSMUSP00000066092; ENSMUSG00000053898.
GeneID	51798.
KEGG	mmu:51798.
UCSC	uc009gab.1. mouse.
Organism-specific databases
CTD	1891.
MGI	MGI:1858208. Ech1.
Phylogenomic databases
eggNOG	COG1024.
GeneTree	ENSGT00700000104254.
HOGENOM	HOG000027939.
HOVERGEN	HBG005556.
InParanoid	O35459.
KO	K12663.
OMA	EIDMGMA.
Enzyme and pathway databases
UniPathway	UPA00659.
Gene expression databases
ArrayExpress	O35459.
Bgee	O35459.
CleanEx	MM_ECH1.
Genevestigator	O35459.
GermOnline	ENSMUSG00000053898. Mus musculus.
Family and domain databases
Gene3D	1.10.12.10. 1 hit.
InterPro	IPR014748. Crontonase_C. IPR001753. Crotonase_core_superfam. IPR018376. Enoyl-CoA_hyd/isom_CS. [Graphical view]
Pfam	PF00378. ECH. 1 hit. [Graphical view]
PROSITE	PS00166. ENOYL_COA_HYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	308044.
SOURCE	Search...

Entry information

Entry name

ECH1_MOUSE

Accession

Primary (citable) accession number: O35459
Secondary accession number(s): Q5M8P6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	April 3, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program