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O35459

- ECH1_MOUSE

UniProt

O35459 - ECH1_MOUSE

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Protein
Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
Gene
Ech1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei173 – 1731Substrate; via amide nitrogen By similarity
Sitei196 – 1961Important for catalytic activity By similarity

GO - Molecular functioni

  1. isomerase activity Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. fatty acid beta-oxidation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial (EC:5.3.3.-)
Gene namesi
Name:Ech1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1858208. Ech1.

Subcellular locationi

Mitochondrion By similarity. Peroxisome By similarity

GO - Cellular componenti

  1. mitochondrion Source: MGI
  2. peroxisome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3333Mitochondrion Reviewed prediction
Add
BLAST
Chaini34 – 327294Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
PRO_0000007418Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei147 – 1471N6-acetyllysine1 Publication
Modified residuei230 – 2301N6-succinyllysine1 Publication
Modified residuei316 – 3161N6-succinyllysine1 Publication
Modified residuei326 – 3261N6-acetyllysine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiO35459.
PaxDbiO35459.
PRIDEiO35459.

2D gel databases

REPRODUCTION-2DPAGEiO35459.

PTM databases

PhosphoSiteiO35459.

Expressioni

Gene expression databases

ArrayExpressiO35459.
BgeeiO35459.
CleanExiMM_ECH1.
GenevestigatoriO35459.

Interactioni

Subunit structurei

Homohexamer By similarity.

Protein-protein interaction databases

IntActiO35459. 4 interactions.
MINTiMINT-1859888.
STRINGi10090.ENSMUSP00000066092.

Structurei

3D structure databases

ProteinModelPortaliO35459.
SMRiO35459. Positions 54-327.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi325 – 3273Microbody targeting signal Reviewed prediction

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1024.
GeneTreeiENSGT00720000108577.
HOGENOMiHOG000027939.
HOVERGENiHBG005556.
InParanoidiO35459.
KOiK12663.
OMAiSWVKDVC.
OrthoDBiEOG7JHM64.
PhylomeDBiO35459.
TreeFamiTF314317.

Family and domain databases

Gene3Di1.10.12.10. 1 hit.
3.90.226.10. 1 hit.
InterProiIPR029045. ClpP/crotonase-like_dom.
IPR014748. Crontonase_C.
IPR001753. Crotonase_core_superfam.
IPR018376. Enoyl-CoA_hyd/isom_CS.
[Graphical view]
PfamiPF00378. ECH. 1 hit.
[Graphical view]
SUPFAMiSSF52096. SSF52096. 1 hit.
PROSITEiPS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35459-1 [UniParc]FASTAAdd to Basket

« Hide

MATAMTVSSK LRGLLMQQLR GTSQLYFNIS LRSLSSSAQE ASKRAPEEVS    50
DHNYESIQVT SAQKHVLHVQ LNRPEKRNAM NRAFWRELVE CFQKISKDSD 100
CRAVVVSGAG KMFTSGIDLM DMASELMQPS GDDAARIAWY LRDLISKYQK 150
TFTVIEKCPK PVIAAIHGGC IGGGVDLVSA CDIRYCTQDA FFQIKEVDMG 200
LAADVGTLQR LPKVIGNQSL VNELTFSARK MMADEALDSG LVSRVFQDKD 250
AMLNAAFALA ADISSKSPVA VQGSKINLIY SRDHSVDESL DYMATWNMSM 300
LQTQDIIKSV QAAMEKRDTK SITFSKL 327
Length:327
Mass (Da):36,118
Last modified:January 1, 1998 - v1
Checksum:iCC0E612C74FBEE2E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF030343 mRNA. Translation: AAB84224.1.
BC068112 mRNA. Translation: AAH68112.1.
BC087924 mRNA. Translation: AAH87924.1.
CCDSiCCDS21057.1.
RefSeqiNP_058052.1. NM_016772.1.
UniGeneiMm.291776.

Genome annotation databases

EnsembliENSMUST00000066264; ENSMUSP00000066092; ENSMUSG00000053898.
GeneIDi51798.
KEGGimmu:51798.
UCSCiuc009gab.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF030343 mRNA. Translation: AAB84224.1 .
BC068112 mRNA. Translation: AAH68112.1 .
BC087924 mRNA. Translation: AAH87924.1 .
CCDSi CCDS21057.1.
RefSeqi NP_058052.1. NM_016772.1.
UniGenei Mm.291776.

3D structure databases

ProteinModelPortali O35459.
SMRi O35459. Positions 54-327.
ModBasei Search...

Protein-protein interaction databases

IntActi O35459. 4 interactions.
MINTi MINT-1859888.
STRINGi 10090.ENSMUSP00000066092.

PTM databases

PhosphoSitei O35459.

2D gel databases

REPRODUCTION-2DPAGEi O35459.

Proteomic databases

MaxQBi O35459.
PaxDbi O35459.
PRIDEi O35459.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000066264 ; ENSMUSP00000066092 ; ENSMUSG00000053898 .
GeneIDi 51798.
KEGGi mmu:51798.
UCSCi uc009gab.1. mouse.

Organism-specific databases

CTDi 1891.
MGIi MGI:1858208. Ech1.

Phylogenomic databases

eggNOGi COG1024.
GeneTreei ENSGT00720000108577.
HOGENOMi HOG000027939.
HOVERGENi HBG005556.
InParanoidi O35459.
KOi K12663.
OMAi SWVKDVC.
OrthoDBi EOG7JHM64.
PhylomeDBi O35459.
TreeFami TF314317.

Enzyme and pathway databases

UniPathwayi UPA00659 .

Miscellaneous databases

NextBioi 308044.
PROi O35459.
SOURCEi Search...

Gene expression databases

ArrayExpressi O35459.
Bgeei O35459.
CleanExi MM_ECH1.
Genevestigatori O35459.

Family and domain databases

Gene3Di 1.10.12.10. 1 hit.
3.90.226.10. 1 hit.
InterProi IPR029045. ClpP/crotonase-like_dom.
IPR014748. Crontonase_C.
IPR001753. Crotonase_core_superfam.
IPR018376. Enoyl-CoA_hyd/isom_CS.
[Graphical view ]
Pfami PF00378. ECH. 1 hit.
[Graphical view ]
SUPFAMi SSF52096. SSF52096. 1 hit.
PROSITEi PS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterisation of mouse ECH1."
    Fitzpatrick D.R.
    Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain and Testis.
  3. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-230 AND LYS-316, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  4. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-147, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiECH1_MOUSE
AccessioniPrimary (citable) accession number: O35459
Secondary accession number(s): Q5M8P6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: July 9, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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