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Protein

Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial

Gene

Ech1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA.By similarity

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei173Substrate; via amide nitrogenBy similarity1
Sitei196Important for catalytic activityBy similarity1
Sitei204Important for catalytic activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial (EC:5.3.3.-)
Gene namesi
Name:Ech1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1858208. Ech1.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • membrane Source: MGI
  • mitochondrion Source: MGI
  • peroxisome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 33MitochondrionSequence analysisAdd BLAST33
ChainiPRO_000000741834 – 327Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrialAdd BLAST294

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei147N6-acetyllysineCombined sources1
Modified residuei230N6-succinyllysineCombined sources1
Modified residuei267PhosphoserineBy similarity1
Modified residuei316N6-succinyllysineCombined sources1
Modified residuei326N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO35459.
MaxQBiO35459.
PaxDbiO35459.
PeptideAtlasiO35459.
PRIDEiO35459.

2D gel databases

REPRODUCTION-2DPAGEO35459.

PTM databases

iPTMnetiO35459.
PhosphoSitePlusiO35459.
SwissPalmiO35459.

Expressioni

Gene expression databases

BgeeiENSMUSG00000053898.
CleanExiMM_ECH1.
ExpressionAtlasiO35459. baseline and differential.
GenevisibleiO35459. MM.

Interactioni

Subunit structurei

Homohexamer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi206181. 1 interactor.
IntActiO35459. 4 interactors.
MINTiMINT-1859888.
STRINGi10090.ENSMUSP00000066092.

Structurei

3D structure databases

ProteinModelPortaliO35459.
SMRiO35459.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni115 – 119Substrate bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi325 – 327Microbody targeting signalSequence analysis3

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1681. Eukaryota.
ENOG410XTHX. LUCA.
GeneTreeiENSGT00760000119100.
HOGENOMiHOG000027939.
HOVERGENiHBG005556.
InParanoidiO35459.
KOiK12663.
OMAiSYMRDHR.
OrthoDBiEOG091G0H26.
PhylomeDBiO35459.
TreeFamiTF314317.

Family and domain databases

Gene3Di1.10.12.10. 1 hit.
3.90.226.10. 1 hit.
InterProiIPR029045. ClpP/crotonase-like_dom.
IPR014748. Crontonase_C.
IPR001753. Crotonase_core_superfam.
IPR018376. Enoyl-CoA_hyd/isom_CS.
[Graphical view]
PfamiPF00378. ECH_1. 1 hit.
[Graphical view]
SUPFAMiSSF52096. SSF52096. 1 hit.
PROSITEiPS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35459-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATAMTVSSK LRGLLMQQLR GTSQLYFNIS LRSLSSSAQE ASKRAPEEVS
60 70 80 90 100
DHNYESIQVT SAQKHVLHVQ LNRPEKRNAM NRAFWRELVE CFQKISKDSD
110 120 130 140 150
CRAVVVSGAG KMFTSGIDLM DMASELMQPS GDDAARIAWY LRDLISKYQK
160 170 180 190 200
TFTVIEKCPK PVIAAIHGGC IGGGVDLVSA CDIRYCTQDA FFQIKEVDMG
210 220 230 240 250
LAADVGTLQR LPKVIGNQSL VNELTFSARK MMADEALDSG LVSRVFQDKD
260 270 280 290 300
AMLNAAFALA ADISSKSPVA VQGSKINLIY SRDHSVDESL DYMATWNMSM
310 320
LQTQDIIKSV QAAMEKRDTK SITFSKL
Length:327
Mass (Da):36,118
Last modified:January 1, 1998 - v1
Checksum:iCC0E612C74FBEE2E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030343 mRNA. Translation: AAB84224.1.
BC068112 mRNA. Translation: AAH68112.1.
BC087924 mRNA. Translation: AAH87924.1.
CCDSiCCDS21057.1.
RefSeqiNP_058052.1. NM_016772.1.
UniGeneiMm.291776.

Genome annotation databases

EnsembliENSMUST00000066264; ENSMUSP00000066092; ENSMUSG00000053898.
GeneIDi51798.
KEGGimmu:51798.
UCSCiuc009gab.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030343 mRNA. Translation: AAB84224.1.
BC068112 mRNA. Translation: AAH68112.1.
BC087924 mRNA. Translation: AAH87924.1.
CCDSiCCDS21057.1.
RefSeqiNP_058052.1. NM_016772.1.
UniGeneiMm.291776.

3D structure databases

ProteinModelPortaliO35459.
SMRiO35459.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206181. 1 interactor.
IntActiO35459. 4 interactors.
MINTiMINT-1859888.
STRINGi10090.ENSMUSP00000066092.

PTM databases

iPTMnetiO35459.
PhosphoSitePlusiO35459.
SwissPalmiO35459.

2D gel databases

REPRODUCTION-2DPAGEO35459.

Proteomic databases

EPDiO35459.
MaxQBiO35459.
PaxDbiO35459.
PeptideAtlasiO35459.
PRIDEiO35459.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000066264; ENSMUSP00000066092; ENSMUSG00000053898.
GeneIDi51798.
KEGGimmu:51798.
UCSCiuc009gab.1. mouse.

Organism-specific databases

CTDi1891.
MGIiMGI:1858208. Ech1.

Phylogenomic databases

eggNOGiKOG1681. Eukaryota.
ENOG410XTHX. LUCA.
GeneTreeiENSGT00760000119100.
HOGENOMiHOG000027939.
HOVERGENiHBG005556.
InParanoidiO35459.
KOiK12663.
OMAiSYMRDHR.
OrthoDBiEOG091G0H26.
PhylomeDBiO35459.
TreeFamiTF314317.

Enzyme and pathway databases

UniPathwayiUPA00659.

Miscellaneous databases

PROiO35459.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000053898.
CleanExiMM_ECH1.
ExpressionAtlasiO35459. baseline and differential.
GenevisibleiO35459. MM.

Family and domain databases

Gene3Di1.10.12.10. 1 hit.
3.90.226.10. 1 hit.
InterProiIPR029045. ClpP/crotonase-like_dom.
IPR014748. Crontonase_C.
IPR001753. Crotonase_core_superfam.
IPR018376. Enoyl-CoA_hyd/isom_CS.
[Graphical view]
PfamiPF00378. ECH_1. 1 hit.
[Graphical view]
SUPFAMiSSF52096. SSF52096. 1 hit.
PROSITEiPS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiECH1_MOUSE
AccessioniPrimary (citable) accession number: O35459
Secondary accession number(s): Q5M8P6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.