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O35458 (VIAAT_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vesicular inhibitory amino acid transporter
Alternative name(s):
GABA and glycine transporter
Solute carrier family 32 member 1
Vesicular GABA transporter
Short name=rGVAT
Gene names
Name:Slc32a1
Synonyms:Vgat, Viaat
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length525 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the uptake of GABA and glycine into the synaptic vesicles.

Subcellular location

Cytoplasmic vesicle membrane; Multi-pass membrane protein.

Tissue specificity

Brain. Expressed at high levels within the neocortex, hippocampus, cerebellum, striatum, septal nuclei and the reticular nucleus of the thalamus. Also expressed in islets where it is more abundant in the peripheral/mantle region.

Sequence similarities

Belongs to the amino acid/polyamine transporter 2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 525525Vesicular inhibitory amino acid transporter
PRO_0000093823

Regions

Topological domain1 – 133133Cytoplasmic Potential
Transmembrane134 – 15421Helical; Potential
Topological domain155 – 20450Lumenal, vesicle Potential
Transmembrane205 – 22521Helical; Potential
Topological domain226 – 24217Cytoplasmic Potential
Transmembrane243 – 26321Helical; Potential
Topological domain264 – 2652Lumenal, vesicle Potential
Transmembrane266 – 28621Helical; Potential
Topological domain287 – 30519Cytoplasmic Potential
Transmembrane306 – 32621Helical; Potential
Topological domain327 – 34115Lumenal, vesicle Potential
Transmembrane342 – 36221Helical; Potential
Topological domain363 – 38321Cytoplasmic Potential
Transmembrane384 – 40421Helical; Potential
Topological domain405 – 43834Lumenal, vesicle Potential
Transmembrane439 – 45921Helical; Potential
Topological domain460 – 4612Cytoplasmic Potential
Transmembrane462 – 48221Helical; Potential
Topological domain483 – 4897Lumenal, vesicle Potential
Transmembrane490 – 51021Helical; Potential
Topological domain511 – 52515Cytoplasmic Potential

Amino acid modifications

Modified residue1861Nitrated tyrosine By similarity
Glycosylation3411N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
O35458 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 33C5E8D31B7BD510

FASTA52557,407
        10         20         30         40         50         60 
MATLLRSKLT NVATSVSNKS QAKVSGMFAR MGFQAATDEE AVGFAHCDDL DFEHRQGLQM 

        70         80         90        100        110        120 
DILKSEGEPC GDEGAEPPVE GDIHYQRGGA PLPPSGSKDQ AVGAGGEFGG HDKPKITAWE 

       130        140        150        160        170        180 
AGWNVTNAIQ GMFVLGLPYA ILHGGYLGLF LIIFAAVVCC YTGKILIACL YEENEDGEVV 

       190        200        210        220        230        240 
RVRDSYVAIA NACCAPRFPT LGGRVVNVAQ IIELVMTCIL YVVVSGNLMY NSFPGLPVSQ 

       250        260        270        280        290        300 
KSWSIIATAV LLPCAFLKNL KAVSKFSLLC TLAHFVINIL VIAYCLSRAR DWAWEKVKFY 

       310        320        330        340        350        360 
IDVKKFPISI GIIVFSYTSQ IFLPSLEGNM QQPSEFHCMM NWTHIAACVL KGLFALVAYL 

       370        380        390        400        410        420 
TWADETKEVI TDNLPGSIRA VVNIFLVAKA LLSYPLPFFA AVEVLEKSLF QEGSRAFFPA 

       430        440        450        460        470        480 
CYGGDGRLKS WGLTLRCALV VFTLLMAIYV PHFALLMGLT GSLTGAGLCF LLPSLFHLRL 

       490        500        510        520 
LWRKLLWHQV FFDVAIFVIG GICSVSGFVH SLEGLIEAYR TNAED 

« Hide

References

[1]"Identification and characterization of the vesicular GABA transporter."
McIntire S.L., Reimer R.J., Schuske K., Edwards R.H., Jorgensen E.M.
Nature 389:870-876(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
Strain: Sprague-Dawley.
[2]"Expression of the vesicular inhibitory amino acid transporter in pancreatic islet cells: distribution of the transporter within rat islets."
Chessler S.D., Simonson W.T., Sweet I.R., Hammerle L.P.
Diabetes 51:1763-1771(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF030253 mRNA. Translation: AAB82950.1.
RefSeqNP_113970.1. NM_031782.1.
XP_006235503.1. XM_006235441.1.
UniGeneRn.10846.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO35458. 1 interaction.
STRING10116.ENSRNOP00000020720.

Proteomic databases

PaxDbO35458.
PRIDEO35458.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000020720; ENSRNOP00000020720; ENSRNOG00000015393.
GeneID83612.
KEGGrno:83612.
UCSCRGD:621402. rat.

Organism-specific databases

CTD140679.
RGD621402. Slc32a1.

Phylogenomic databases

eggNOGNOG288356.
GeneTreeENSGT00490000043380.
HOGENOMHOG000220837.
HOVERGENHBG061364.
InParanoidO35458.
KOK15015.
OMAMYNSFPN.
OrthoDBEOG7SR4M2.
PhylomeDBO35458.
TreeFamTF312818.

Gene expression databases

GenevestigatorO35458.

Family and domain databases

InterProIPR013057. AA_transpt_TM.
[Graphical view]
PfamPF01490. Aa_trans. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio616151.
PROO35458.

Entry information

Entry nameVIAAT_RAT
AccessionPrimary (citable) accession number: O35458
Entry history
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families