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Protein

Vesicular inhibitory amino acid transporter

Gene

Slc32a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the uptake of GABA and glycine into the synaptic vesicles.

GO - Molecular functioni

GO - Biological processi

  • aging Source: RGD
  • amino acid transmembrane transport Source: GO_Central
  • glycine transport Source: RGD
  • hippocampus development Source: RGD
  • neurotransmitter transport Source: RGD
Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Transport

Enzyme and pathway databases

ReactomeiREACT_289838. GABA synthesis, release, reuptake and degradation.
REACT_335804. Transport of inorganic cations/anions and amino acids/oligopeptides.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicular inhibitory amino acid transporter
Alternative name(s):
GABA and glycine transporter
Solute carrier family 32 member 1
Vesicular GABA transporter
Short name:
rGVAT
Gene namesi
Name:Slc32a1
Synonyms:Vgat, Viaat
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi621402. Slc32a1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 133133CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei134 – 15421HelicalSequence AnalysisAdd
BLAST
Topological domaini155 – 20450Lumenal, vesicleSequence AnalysisAdd
BLAST
Transmembranei205 – 22521HelicalSequence AnalysisAdd
BLAST
Topological domaini226 – 24217CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei243 – 26321HelicalSequence AnalysisAdd
BLAST
Topological domaini264 – 2652Lumenal, vesicleSequence Analysis
Transmembranei266 – 28621HelicalSequence AnalysisAdd
BLAST
Topological domaini287 – 30519CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei306 – 32621HelicalSequence AnalysisAdd
BLAST
Topological domaini327 – 34115Lumenal, vesicleSequence AnalysisAdd
BLAST
Transmembranei342 – 36221HelicalSequence AnalysisAdd
BLAST
Topological domaini363 – 38321CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei384 – 40421HelicalSequence AnalysisAdd
BLAST
Topological domaini405 – 43834Lumenal, vesicleSequence AnalysisAdd
BLAST
Transmembranei439 – 45921HelicalSequence AnalysisAdd
BLAST
Topological domaini460 – 4612CytoplasmicSequence Analysis
Transmembranei462 – 48221HelicalSequence AnalysisAdd
BLAST
Topological domaini483 – 4897Lumenal, vesicleSequence Analysis
Transmembranei490 – 51021HelicalSequence AnalysisAdd
BLAST
Topological domaini511 – 52515CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • cell tip Source: Ensembl
  • cone cell pedicle Source: Ensembl
  • cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  • dendrite terminus Source: Ensembl
  • inhibitory synapse Source: BHF-UCL
  • integral component of membrane Source: RGD
  • intracellular organelle Source: RGD
  • presynaptic active zone Source: Ensembl
  • synapse Source: MGI
  • synaptic vesicle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 525525Vesicular inhibitory amino acid transporterPRO_0000093823Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei186 – 1861Nitrated tyrosineBy similarity
Glycosylationi341 – 3411N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein, Nitration

Proteomic databases

PaxDbiO35458.
PRIDEiO35458.

Expressioni

Tissue specificityi

Brain. Expressed at high levels within the neocortex, hippocampus, cerebellum, striatum, septal nuclei and the reticular nucleus of the thalamus. Also expressed in islets where it is more abundant in the peripheral/mantle region.

Gene expression databases

GenevisibleiO35458. RN.

Interactioni

Protein-protein interaction databases

IntActiO35458. 1 interaction.
STRINGi10116.ENSRNOP00000020720.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG288356.
GeneTreeiENSGT00490000043380.
HOGENOMiHOG000220837.
HOVERGENiHBG061364.
InParanoidiO35458.
KOiK15015.
OMAiKTEGEPC.
OrthoDBiEOG7SR4M2.
PhylomeDBiO35458.
TreeFamiTF312818.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35458-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLLRSKLT NVATSVSNKS QAKVSGMFAR MGFQAATDEE AVGFAHCDDL
60 70 80 90 100
DFEHRQGLQM DILKSEGEPC GDEGAEPPVE GDIHYQRGGA PLPPSGSKDQ
110 120 130 140 150
AVGAGGEFGG HDKPKITAWE AGWNVTNAIQ GMFVLGLPYA ILHGGYLGLF
160 170 180 190 200
LIIFAAVVCC YTGKILIACL YEENEDGEVV RVRDSYVAIA NACCAPRFPT
210 220 230 240 250
LGGRVVNVAQ IIELVMTCIL YVVVSGNLMY NSFPGLPVSQ KSWSIIATAV
260 270 280 290 300
LLPCAFLKNL KAVSKFSLLC TLAHFVINIL VIAYCLSRAR DWAWEKVKFY
310 320 330 340 350
IDVKKFPISI GIIVFSYTSQ IFLPSLEGNM QQPSEFHCMM NWTHIAACVL
360 370 380 390 400
KGLFALVAYL TWADETKEVI TDNLPGSIRA VVNIFLVAKA LLSYPLPFFA
410 420 430 440 450
AVEVLEKSLF QEGSRAFFPA CYGGDGRLKS WGLTLRCALV VFTLLMAIYV
460 470 480 490 500
PHFALLMGLT GSLTGAGLCF LLPSLFHLRL LWRKLLWHQV FFDVAIFVIG
510 520
GICSVSGFVH SLEGLIEAYR TNAED
Length:525
Mass (Da):57,407
Last modified:January 1, 1998 - v1
Checksum:i33C5E8D31B7BD510
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030253 mRNA. Translation: AAB82950.1.
RefSeqiNP_113970.1. NM_031782.1.
XP_006235503.1. XM_006235441.2.
UniGeneiRn.10846.

Genome annotation databases

EnsembliENSRNOT00000020720; ENSRNOP00000020720; ENSRNOG00000015393.
GeneIDi83612.
KEGGirno:83612.
UCSCiRGD:621402. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030253 mRNA. Translation: AAB82950.1.
RefSeqiNP_113970.1. NM_031782.1.
XP_006235503.1. XM_006235441.2.
UniGeneiRn.10846.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO35458. 1 interaction.
STRINGi10116.ENSRNOP00000020720.

Proteomic databases

PaxDbiO35458.
PRIDEiO35458.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000020720; ENSRNOP00000020720; ENSRNOG00000015393.
GeneIDi83612.
KEGGirno:83612.
UCSCiRGD:621402. rat.

Organism-specific databases

CTDi140679.
RGDi621402. Slc32a1.

Phylogenomic databases

eggNOGiNOG288356.
GeneTreeiENSGT00490000043380.
HOGENOMiHOG000220837.
HOVERGENiHBG061364.
InParanoidiO35458.
KOiK15015.
OMAiKTEGEPC.
OrthoDBiEOG7SR4M2.
PhylomeDBiO35458.
TreeFamiTF312818.

Enzyme and pathway databases

ReactomeiREACT_289838. GABA synthesis, release, reuptake and degradation.
REACT_335804. Transport of inorganic cations/anions and amino acids/oligopeptides.

Miscellaneous databases

NextBioi616151.
PROiO35458.

Gene expression databases

GenevisibleiO35458. RN.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification and characterization of the vesicular GABA transporter."
    McIntire S.L., Reimer R.J., Schuske K., Edwards R.H., Jorgensen E.M.
    Nature 389:870-876(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
    Strain: Sprague-Dawley.
  2. "Expression of the vesicular inhibitory amino acid transporter in pancreatic islet cells: distribution of the transporter within rat islets."
    Chessler S.D., Simonson W.T., Sweet I.R., Hammerle L.P.
    Diabetes 51:1763-1771(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Tissue: Brain.

Entry informationi

Entry nameiVIAAT_RAT
AccessioniPrimary (citable) accession number: O35458
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: January 1, 1998
Last modified: July 22, 2015
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.