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O35458

- VIAAT_RAT

UniProt

O35458 - VIAAT_RAT

Protein

Vesicular inhibitory amino acid transporter

Gene

Slc32a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Involved in the uptake of GABA and glycine into the synaptic vesicles.

    GO - Molecular functioni

    1. gamma-aminobutyric acid:proton symporter activity Source: Ensembl
    2. glycine transmembrane transporter activity Source: RGD

    GO - Biological processi

    1. aging Source: RGD
    2. glycine transport Source: RGD
    3. neurotransmitter transport Source: RGD

    Keywords - Biological processi

    Neurotransmitter transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vesicular inhibitory amino acid transporter
    Alternative name(s):
    GABA and glycine transporter
    Solute carrier family 32 member 1
    Vesicular GABA transporter
    Short name:
    rGVAT
    Gene namesi
    Name:Slc32a1
    Synonyms:Vgat, Viaat
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 3

    Organism-specific databases

    RGDi621402. Slc32a1.

    Subcellular locationi

    GO - Cellular componenti

    1. cell tip Source: Ensembl
    2. cone cell pedicle Source: Ensembl
    3. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
    4. dendrite terminus Source: Ensembl
    5. inhibitory synapse Source: BHF-UCL
    6. integral component of membrane Source: RGD
    7. intracellular organelle Source: RGD
    8. synapse Source: MGI
    9. synaptic vesicle Source: RGD

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 525525Vesicular inhibitory amino acid transporterPRO_0000093823Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei186 – 1861Nitrated tyrosineBy similarity
    Glycosylationi341 – 3411N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein, Nitration

    Proteomic databases

    PaxDbiO35458.
    PRIDEiO35458.

    Expressioni

    Tissue specificityi

    Brain. Expressed at high levels within the neocortex, hippocampus, cerebellum, striatum, septal nuclei and the reticular nucleus of the thalamus. Also expressed in islets where it is more abundant in the peripheral/mantle region.

    Gene expression databases

    GenevestigatoriO35458.

    Interactioni

    Protein-protein interaction databases

    IntActiO35458. 1 interaction.
    STRINGi10116.ENSRNOP00000020720.

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 133133CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini155 – 20450Lumenal, vesicleSequence AnalysisAdd
    BLAST
    Topological domaini226 – 24217CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini264 – 2652Lumenal, vesicleSequence Analysis
    Topological domaini287 – 30519CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini327 – 34115Lumenal, vesicleSequence AnalysisAdd
    BLAST
    Topological domaini363 – 38321CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini405 – 43834Lumenal, vesicleSequence AnalysisAdd
    BLAST
    Topological domaini460 – 4612CytoplasmicSequence Analysis
    Topological domaini483 – 4897Lumenal, vesicleSequence Analysis
    Topological domaini511 – 52515CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei134 – 15421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei205 – 22521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei243 – 26321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei266 – 28621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei306 – 32621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei342 – 36221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei384 – 40421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei439 – 45921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei462 – 48221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei490 – 51021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG288356.
    GeneTreeiENSGT00490000043380.
    HOGENOMiHOG000220837.
    HOVERGENiHBG061364.
    InParanoidiO35458.
    KOiK15015.
    OMAiMYNSFPN.
    OrthoDBiEOG7SR4M2.
    PhylomeDBiO35458.
    TreeFamiTF312818.

    Family and domain databases

    InterProiIPR013057. AA_transpt_TM.
    [Graphical view]
    PfamiPF01490. Aa_trans. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O35458-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATLLRSKLT NVATSVSNKS QAKVSGMFAR MGFQAATDEE AVGFAHCDDL    50
    DFEHRQGLQM DILKSEGEPC GDEGAEPPVE GDIHYQRGGA PLPPSGSKDQ 100
    AVGAGGEFGG HDKPKITAWE AGWNVTNAIQ GMFVLGLPYA ILHGGYLGLF 150
    LIIFAAVVCC YTGKILIACL YEENEDGEVV RVRDSYVAIA NACCAPRFPT 200
    LGGRVVNVAQ IIELVMTCIL YVVVSGNLMY NSFPGLPVSQ KSWSIIATAV 250
    LLPCAFLKNL KAVSKFSLLC TLAHFVINIL VIAYCLSRAR DWAWEKVKFY 300
    IDVKKFPISI GIIVFSYTSQ IFLPSLEGNM QQPSEFHCMM NWTHIAACVL 350
    KGLFALVAYL TWADETKEVI TDNLPGSIRA VVNIFLVAKA LLSYPLPFFA 400
    AVEVLEKSLF QEGSRAFFPA CYGGDGRLKS WGLTLRCALV VFTLLMAIYV 450
    PHFALLMGLT GSLTGAGLCF LLPSLFHLRL LWRKLLWHQV FFDVAIFVIG 500
    GICSVSGFVH SLEGLIEAYR TNAED 525
    Length:525
    Mass (Da):57,407
    Last modified:January 1, 1998 - v1
    Checksum:i33C5E8D31B7BD510
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF030253 mRNA. Translation: AAB82950.1.
    RefSeqiNP_113970.1. NM_031782.1.
    XP_006235503.1. XM_006235441.1.
    UniGeneiRn.10846.

    Genome annotation databases

    EnsembliENSRNOT00000020720; ENSRNOP00000020720; ENSRNOG00000015393.
    GeneIDi83612.
    KEGGirno:83612.
    UCSCiRGD:621402. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF030253 mRNA. Translation: AAB82950.1 .
    RefSeqi NP_113970.1. NM_031782.1.
    XP_006235503.1. XM_006235441.1.
    UniGenei Rn.10846.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O35458. 1 interaction.
    STRINGi 10116.ENSRNOP00000020720.

    Proteomic databases

    PaxDbi O35458.
    PRIDEi O35458.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000020720 ; ENSRNOP00000020720 ; ENSRNOG00000015393 .
    GeneIDi 83612.
    KEGGi rno:83612.
    UCSCi RGD:621402. rat.

    Organism-specific databases

    CTDi 140679.
    RGDi 621402. Slc32a1.

    Phylogenomic databases

    eggNOGi NOG288356.
    GeneTreei ENSGT00490000043380.
    HOGENOMi HOG000220837.
    HOVERGENi HBG061364.
    InParanoidi O35458.
    KOi K15015.
    OMAi MYNSFPN.
    OrthoDBi EOG7SR4M2.
    PhylomeDBi O35458.
    TreeFami TF312818.

    Miscellaneous databases

    NextBioi 616151.
    PROi O35458.

    Gene expression databases

    Genevestigatori O35458.

    Family and domain databases

    InterProi IPR013057. AA_transpt_TM.
    [Graphical view ]
    Pfami PF01490. Aa_trans. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of the vesicular GABA transporter."
      McIntire S.L., Reimer R.J., Schuske K., Edwards R.H., Jorgensen E.M.
      Nature 389:870-876(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
      Strain: Sprague-Dawley.
    2. "Expression of the vesicular inhibitory amino acid transporter in pancreatic islet cells: distribution of the transporter within rat islets."
      Chessler S.D., Simonson W.T., Sweet I.R., Hammerle L.P.
      Diabetes 51:1763-1771(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Tissue: Brain.

    Entry informationi

    Entry nameiVIAAT_RAT
    AccessioniPrimary (citable) accession number: O35458
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 28, 2003
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3