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Protein

Vesicular inhibitory amino acid transporter

Gene

Slc32a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the uptake of GABA and glycine into the synaptic vesicles.1 Publication

GO - Molecular functioni

GO - Biological processi

  • aging Source: RGD
  • glycine transport Source: RGD
  • hippocampus development Source: RGD
  • neurotransmitter transport Source: RGD
Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-425393. Transport of inorganic cations/anions and amino acids/oligopeptides.
R-RNO-888590. GABA synthesis, release, reuptake and degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicular inhibitory amino acid transporter
Alternative name(s):
GABA and glycine transporter
Solute carrier family 32 member 1
Vesicular GABA transporter
Short name:
rGVAT
Gene namesi
Name:Slc32a1
Synonyms:Vgat, Viaat
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi621402. Slc32a1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 133CytoplasmicSequence analysisAdd BLAST133
Transmembranei134 – 154HelicalSequence analysisAdd BLAST21
Topological domaini155 – 204Lumenal, vesicleSequence analysisAdd BLAST50
Transmembranei205 – 225HelicalSequence analysisAdd BLAST21
Topological domaini226 – 242CytoplasmicSequence analysisAdd BLAST17
Transmembranei243 – 263HelicalSequence analysisAdd BLAST21
Topological domaini264 – 265Lumenal, vesicleSequence analysis2
Transmembranei266 – 286HelicalSequence analysisAdd BLAST21
Topological domaini287 – 305CytoplasmicSequence analysisAdd BLAST19
Transmembranei306 – 326HelicalSequence analysisAdd BLAST21
Topological domaini327 – 341Lumenal, vesicleSequence analysisAdd BLAST15
Transmembranei342 – 362HelicalSequence analysisAdd BLAST21
Topological domaini363 – 383CytoplasmicSequence analysisAdd BLAST21
Transmembranei384 – 404HelicalSequence analysisAdd BLAST21
Topological domaini405 – 438Lumenal, vesicleSequence analysisAdd BLAST34
Transmembranei439 – 459HelicalSequence analysisAdd BLAST21
Topological domaini460 – 461CytoplasmicSequence analysis2
Transmembranei462 – 482HelicalSequence analysisAdd BLAST21
Topological domaini483 – 489Lumenal, vesicleSequence analysis7
Transmembranei490 – 510HelicalSequence analysisAdd BLAST21
Topological domaini511 – 525CytoplasmicSequence analysisAdd BLAST15

GO - Cellular componenti

  • cell tip Source: Ensembl
  • cone cell pedicle Source: Ensembl
  • cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  • dendrite terminus Source: Ensembl
  • inhibitory synapse Source: BHF-UCL
  • integral component of membrane Source: RGD
  • intracellular organelle Source: RGD
  • presynaptic active zone Source: Ensembl
  • synapse Source: MGI
  • synaptic vesicle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000938231 – 525Vesicular inhibitory amino acid transporterAdd BLAST525

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei186Nitrated tyrosineBy similarity1
Glycosylationi341N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein, Nitration

Proteomic databases

PaxDbiO35458.
PRIDEiO35458.

PTM databases

SwissPalmiO35458.

Expressioni

Tissue specificityi

Brain. Expressed at high levels within the neocortex, hippocampus, cerebellum, striatum, septal nuclei and the reticular nucleus of the thalamus (PubMed:9349821). Also expressed in islets where it is more abundant in the peripheral/mantle region (PubMed:12031963).2 Publications

Gene expression databases

BgeeiENSRNOG00000015393.
GenevisibleiO35458. RN.

Interactioni

Protein-protein interaction databases

IntActiO35458. 1 interactor.
STRINGi10116.ENSRNOP00000020720.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4303. Eukaryota.
COG0814. LUCA.
GeneTreeiENSGT00490000043380.
HOGENOMiHOG000220837.
HOVERGENiHBG061364.
InParanoidiO35458.
KOiK15015.
OMAiANACCSP.
OrthoDBiEOG091G05MY.
PhylomeDBiO35458.
TreeFamiTF312818.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35458-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLLRSKLT NVATSVSNKS QAKVSGMFAR MGFQAATDEE AVGFAHCDDL
60 70 80 90 100
DFEHRQGLQM DILKSEGEPC GDEGAEPPVE GDIHYQRGGA PLPPSGSKDQ
110 120 130 140 150
AVGAGGEFGG HDKPKITAWE AGWNVTNAIQ GMFVLGLPYA ILHGGYLGLF
160 170 180 190 200
LIIFAAVVCC YTGKILIACL YEENEDGEVV RVRDSYVAIA NACCAPRFPT
210 220 230 240 250
LGGRVVNVAQ IIELVMTCIL YVVVSGNLMY NSFPGLPVSQ KSWSIIATAV
260 270 280 290 300
LLPCAFLKNL KAVSKFSLLC TLAHFVINIL VIAYCLSRAR DWAWEKVKFY
310 320 330 340 350
IDVKKFPISI GIIVFSYTSQ IFLPSLEGNM QQPSEFHCMM NWTHIAACVL
360 370 380 390 400
KGLFALVAYL TWADETKEVI TDNLPGSIRA VVNIFLVAKA LLSYPLPFFA
410 420 430 440 450
AVEVLEKSLF QEGSRAFFPA CYGGDGRLKS WGLTLRCALV VFTLLMAIYV
460 470 480 490 500
PHFALLMGLT GSLTGAGLCF LLPSLFHLRL LWRKLLWHQV FFDVAIFVIG
510 520
GICSVSGFVH SLEGLIEAYR TNAED
Length:525
Mass (Da):57,407
Last modified:January 1, 1998 - v1
Checksum:i33C5E8D31B7BD510
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030253 mRNA. Translation: AAB82950.1.
RefSeqiNP_113970.1. NM_031782.1.
XP_006235503.1. XM_006235441.3.
UniGeneiRn.10846.

Genome annotation databases

EnsembliENSRNOT00000020720; ENSRNOP00000020720; ENSRNOG00000015393.
GeneIDi83612.
KEGGirno:83612.
UCSCiRGD:621402. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030253 mRNA. Translation: AAB82950.1.
RefSeqiNP_113970.1. NM_031782.1.
XP_006235503.1. XM_006235441.3.
UniGeneiRn.10846.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO35458. 1 interactor.
STRINGi10116.ENSRNOP00000020720.

PTM databases

SwissPalmiO35458.

Proteomic databases

PaxDbiO35458.
PRIDEiO35458.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000020720; ENSRNOP00000020720; ENSRNOG00000015393.
GeneIDi83612.
KEGGirno:83612.
UCSCiRGD:621402. rat.

Organism-specific databases

CTDi140679.
RGDi621402. Slc32a1.

Phylogenomic databases

eggNOGiKOG4303. Eukaryota.
COG0814. LUCA.
GeneTreeiENSGT00490000043380.
HOGENOMiHOG000220837.
HOVERGENiHBG061364.
InParanoidiO35458.
KOiK15015.
OMAiANACCSP.
OrthoDBiEOG091G05MY.
PhylomeDBiO35458.
TreeFamiTF312818.

Enzyme and pathway databases

ReactomeiR-RNO-425393. Transport of inorganic cations/anions and amino acids/oligopeptides.
R-RNO-888590. GABA synthesis, release, reuptake and degradation.

Miscellaneous databases

PROiO35458.

Gene expression databases

BgeeiENSRNOG00000015393.
GenevisibleiO35458. RN.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVIAAT_RAT
AccessioniPrimary (citable) accession number: O35458
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.