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O35457 (CCRL2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
C-C chemokine receptor-like 2
Alternative name(s):
Chemokine receptor CCR11
G-protein coupled beta chemokine receptor
Lipopolysaccharide-inducible C-C chemokine receptor
Short name=L-CCR
Gene names
Name:Ccrl2
Synonyms:Ccr11, Lccr
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length360 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for CCL19 and chemerin/RARRES2. Does not appear to be a signaling receptor, but may have a role in modulating chemokine-triggered immune responses by capturing and internalizing CCL19 or by presenting RARRES2 ligand to CMKLR1, a functional signaling receptor. Plays a critical role for the development of Th2 responses By similarity. Ref.7 Ref.10 Ref.11

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Expressed in macrophages, astrocytes, in glial cells. Constitutively expressed by mast cells. Detected in bronchial epithelium in OVA-induced airway inflammation. Up-regulated during dendritic cell (DC) maturation. Ref.1 Ref.2 Ref.8 Ref.9 Ref.11

Induction

By bacterial lipopolysaccharide in astrocytes. Ref.2

Domain

Lacks the conserved DRYLAIV motif in the second intracellular loop that is required for signaling of functional chemokine receptors By similarity.

Disruption phenotype

No visible phenotype. Deficient-mice have normal numbers of mast cells in all tissues analyzed. Wild-type and deficient mice develop marked local inflammation when sensitized with a high dose of DNP-specific IgE, however deficient mice have significantly reduced IgE-dependent passive cutaneous anaphylaxis (PCA) reactions when lower sensitizing dose is used. Deficient-mice show normal recruitment of circulating DC into the lung, but a defective trafficking of antigen-loaded lung DC to mediastinal lymph nodes. This defect was associated to a reduction in lymph node cellularity and reduced priming of T-helper cell 2 response. Ref.10 Ref.11

Miscellaneous

It was initially reported that CCRL2 responds functionally to CCL2, CCL5, CCL7, and CCL8 via intracellular calcium mobilization and transwell chemotaxis although no evidence for a direct ligand-receptor interaction was provided in this report (Ref.8). These results are now controversial and other studies failed to confirm CCRL2 recognition and transwell chemotaxis of these chemokines or a series of other CC- and CXC-chemokines using CCRL2-transfected cells (Ref.10).

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 360360C-C chemokine receptor-like 2
PRO_0000236800

Regions

Topological domain1 – 4242Extracellular Potential
Transmembrane43 – 6321Helical; Name=1; Potential
Topological domain64 – 7310Cytoplasmic Potential
Transmembrane74 – 9421Helical; Name=2; Potential
Topological domain95 – 10915Extracellular Potential
Transmembrane110 – 13021Helical; Name=3; Potential
Topological domain131 – 14111Cytoplasmic Potential
Transmembrane142 – 16221Helical; Name=4; Potential
Topological domain163 – 20240Extracellular Potential
Transmembrane203 – 22321Helical; Name=5; Potential
Topological domain224 – 24320Cytoplasmic Potential
Transmembrane244 – 26421Helical; Name=6; Potential
Topological domain265 – 28521Extracellular Potential
Transmembrane286 – 30722Helical; Name=7; Potential
Topological domain308 – 36053Cytoplasmic Potential

Amino acid modifications

Glycosylation31N-linked (GlcNAc...) Potential
Glycosylation1051N-linked (GlcNAc...) Potential
Disulfide bond108 ↔ 185 By similarity

Experimental info

Sequence conflict371P → S in BAE26775. Ref.4
Sequence conflict391Q → R in CAC79612. Ref.2
Sequence conflict1571I → V in AAB86479. Ref.3
Sequence conflict2151F → L in CAC79612. Ref.2
Sequence conflict2921V → A in CAC79612. Ref.2
Sequence conflict3341Y → S in AAB86479. Ref.3
Sequence conflict3411E → K in AAB86479. Ref.3
Sequence conflict3591I → K in CAJ18456. Ref.5

Sequences

Sequence LengthMass (Da)Tools
O35457 [UniParc].

Last modified May 30, 2006. Version 2.
Checksum: E0DBF990A8595E3B

FASTA36040,745
        10         20         30         40         50         60 
MDNYTVAPDD EYDVLILDDY LDNSGPDQVP APEFLSPQQV LQFCCAVFAV GLLDNVLAVF 

        70         80         90        100        110        120 
ILVKYKGLKN LGNIYFLNLA LSNLCFLLPL PFWAHTAAHG ESPGNGTCKV LVGLHSSGLY 

       130        140        150        160        170        180 
SEVFSNILLL VQGYRVFSQG RLASIFTTVS CGIVACILAW AMATALSLPE SVFYEPRMER 

       190        200        210        220        230        240 
QKHKCAFGKP HFLPIEAPLW KYVLTSKMII LVLAFPLLVF IICCRQLRRR QSFRERQYDL 

       250        260        270        280        290        300 
HKPALVITGV FLLMWAPYNT VLFLSAFQEH LSLQDEKSSY HLDASVQVTQ LVATTHCCVN 

       310        320        330        340        350        360 
PLLYLLLDRK AFMRYLRSLF PRCNDIPYQS SGGYQQAPPR EGHGRPIELY SNLHQRQDII 

« Hide

References

« Hide 'large scale' references
[1]"A novel lipopolysaccharide inducible C-C chemokine receptor related gene in murine macrophages."
Shimada T., Matsumoto M., Tatsumi Y., Kanamaru A., Akira S.
FEBS Lett. 425:490-494(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]"LPS-induced expression of a novel chemokine receptor (L-CCR) in mouse glial cells in vitro and in vivo."
Zuurman M.W., Heeroma J., Brouwer N., Boddeke H.W., Biber K.P.H.
Glia 41:327-336(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, TISSUE SPECIFICITY.
Strain: CD-1.
[3]"RANTES and eotaxin stimulate chemotaxis, chemokine/cytokine synthesis, and receptor modulation in murine astroctyes."
Luo Y., Berman M.A., Fischer F.R., Abromson-Leeman S.R., Kuziel W.A., Gerard C., Dorf M.E.
Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: 129/Sv and C57BL/10.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Placenta.
[5]"Cloning of mouse full open reading frames in Gateway(R) system entry vector (pDONR201)."
Ebert L., Muenstermann E., Schatten R., Henze S., Bohn E., Mollenhauer J., Wiemann S., Schick M., Korn B.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Mammary gland.
[7]"Expression of L-CCR in HEK 293 cells reveals functional responses to CCL2, CCL5, CCL7, and CCL8."
Biber K., Zuurman M.W., Homan H., Boddeke H.W.G.M.
J. Leukoc. Biol. 74:243-251(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Induction of glial L-CCR mRNA expression in spinal cord and brain in experimental autoimmune encephalomyelitis."
Brouwer N., Zuurman M.W., Wei T., Ransohoff R.M., Boddeke H.W., Biber K.
Glia 46:84-94(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[9]"Localization and enhanced mRNA expression of the orphan chemokine receptor L-CCR in the lung in a murine model of ovalbumin-induced airway inflammation."
Oostendorp J., Hylkema M.N., Luinge M., Geerlings M., Meurs H., Timens W., Zaagsma J., Postma D.S., Boddeke H.W., Biber K.
J. Histochem. Cytochem. 52:401-410(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[10]"Mast cell-expressed orphan receptor CCRL2 binds chemerin and is required for optimal induction of IgE-mediated passive cutaneous anaphylaxis."
Zabel B.A., Nakae S., Zuniga L., Kim J.Y., Ohyama T., Alt C., Pan J., Suto H., Soler D., Allen S.J., Handel T.M., Song C.H., Galli S.J., Butcher E.C.
J. Exp. Med. 205:2207-2220(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: LIGAND-BINDING, DISRUPTION PHENOTYPE, FUNCTION, LACK OF RESPOND TO CCL2; CCL5; CCL7 AND CCL8.
[11]"Nonredundant role of CCRL2 in lung dendritic cell trafficking."
Otero K., Vecchi A., Hirsch E., Kearley J., Vermi W., Del Prete A., Gonzalvo-Feo S., Garlanda C., Azzolino O., Salogni L., Lloyd C.M., Facchetti F., Mantovani A., Sozzani S.
Blood 116:2942-2949(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB009384 mRNA. Translation: BAA25879.1.
AJ318863 mRNA. Translation: CAC79612.1.
AF316576 mRNA. Translation: AAG30950.1.
AF030185 mRNA. Translation: AAB86479.1.
AK007808 mRNA. Translation: BAB25273.1.
AK145946 mRNA. Translation: BAE26775.1.
AK151856 mRNA. Translation: BAE30745.1.
CT010248 mRNA. Translation: CAJ18456.1.
BC038631 mRNA. Translation: AAH38631.1.
RefSeqNP_059494.2. NM_017466.4.
XP_006512248.1. XM_006512185.1.
XP_006512249.1. XM_006512186.1.
UniGeneMm.7336.

3D structure databases

ProteinModelPortalO35457.
SMRO35457. Positions 47-320.
ModBaseSearch...
MobiDBSearch...

Chemistry

GuidetoPHARMACOLOGY78.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteO35457.

Proteomic databases

PRIDEO35457.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000111888; ENSMUSP00000107519; ENSMUSG00000043953.
GeneID54199.
KEGGmmu:54199.
UCSCuc009rvk.1. mouse.

Organism-specific databases

CTD9034.
MGIMGI:1920904. Ccrl2.

Phylogenomic databases

eggNOGNOG149863.
GeneTreeENSGT00700000104407.
HOGENOMHOG000234122.
HOVERGENHBG106917.
InParanoidO35457.
KOK08373.
OMAFLLMWAP.
OrthoDBEOG77HDFH.
PhylomeDBO35457.
TreeFamTF330966.

Gene expression databases

BgeeO35457.
CleanExMM_CCRL2.
GenevestigatorO35457.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000355. Chemokine_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERPTHR24227. PTHR24227. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00657. CCCHEMOKINER.
PR00237. GPCRRHODOPSN.
PROSITEPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio311054.
PROO35457.
SOURCESearch...

Entry information

Entry nameCCRL2_MOUSE
AccessionPrimary (citable) accession number: O35457
Secondary accession number(s): O70171 expand/collapse secondary AC list , Q3UKM6, Q4FK04, Q91YD7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: May 30, 2006
Last modified: April 16, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries