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O35454

- CLCN6_MOUSE

UniProt

O35454 - CLCN6_MOUSE

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Protein
Chloride transport protein 6
Gene
Clcn6, Clc6
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Chloride transport protein, initially identified as voltage-gated chloride channel. The presence of the conserved gating glutamate residues suggests that is functions as antiporter.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei157 – 1571Chloride By similarity
Sitei200 – 2001Mediates proton transfer from the outer aqueous phase to the interior of the protein; involved in linking H(+) and Cl(-) transport By similarity
Sitei267 – 2671Mediates proton transfer from the protein to the inner aqueous phase By similarity
Binding sitei490 – 4901Chloride; via amide nitrogen By similarity
Binding sitei577 – 5771Chloride By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi631 – 6333ATP By similarity
Nucleotide bindingi850 – 8534ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. antiporter activity Source: UniProtKB-KW
  3. voltage-gated chloride channel activity Source: InterPro

GO - Biological processi

  1. response to mechanical stimulus Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Chloride, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_196640. Stimuli-sensing channels.

Protein family/group databases

TCDBi2.A.49.3.4. the chloride carrier/channel (clc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride transport protein 6
Alternative name(s):
Chloride channel protein 6
Short name:
ClC-6
Gene namesi
Name:Clcn6
Synonyms:Clc6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:1347049. Clcn6.

Subcellular locationi

Endosome membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 8080Cytoplasmic By similarity
Add
BLAST
Transmembranei81 – 11333Helical; By similarity
Add
BLAST
Transmembranei128 – 15023Helical; By similarity
Add
BLAST
Intramembranei159 – 1668Helical; By similarity
Transmembranei176 – 19419Helical; By similarity
Add
BLAST
Transmembranei200 – 21718Helical; By similarity
Add
BLAST
Intramembranei241 – 25313Helical; By similarity
Add
BLAST
Intramembranei257 – 2659Helical; By similarity
Transmembranei277 – 29418Helical; By similarity
Add
BLAST
Transmembranei335 – 36430Helical; By similarity
Add
BLAST
Transmembranei371 – 39222Helical; By similarity
Add
BLAST
Transmembranei463 – 48220Helical; By similarity
Add
BLAST
Transmembranei488 – 51225Helical; By similarity
Add
BLAST
Intramembranei520 – 53415Helical; By similarity
Add
BLAST
Intramembranei535 – 5373Note=Loop between two helices; By similarity
Intramembranei538 – 54912Helical; By similarity
Add
BLAST
Intramembranei550 – 5534Note=Loop between two helices; By similarity
Transmembranei554 – 57219Helical; By similarity
Add
BLAST
Topological domaini573 – 870298Cytoplasmic By similarity
Add
BLAST

GO - Cellular componenti

  1. endosome membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. lysosomal membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

Pathology & Biotechi

Disruption phenotypei

Reduced pain sensitivity and moderate behavioral abnormalities, but have normal fertility and are generally not very different from wild-type.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 870870Chloride transport protein 6
PRO_0000094450Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi410 – 4101N-linked (GlcNAc...) Reviewed prediction
Glycosylationi423 – 4231N-linked (GlcNAc...) Reviewed prediction
Glycosylationi433 – 4331N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

N-glycosylated on several asparagine residues By similarity.

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO35454.
PRIDEiO35454.

PTM databases

PhosphoSiteiO35454.

Expressioni

Tissue specificityi

Detected in whole brain and in hippocampus neurons (at protein level). Detected in brain, trigeminus, dorsal root ganglion, spinal cord, eye, kidney, testis, skeletal muscle, thymus and pancreas. Isoform ClC-6c is expressed only in kidney.2 Publications

Gene expression databases

ArrayExpressiO35454.
BgeeiO35454.
CleanExiMM_CLCN6.
GenevestigatoriO35454.

Interactioni

Protein-protein interaction databases

IntActiO35454. 1 interaction.
MINTiMINT-4090929.

Structurei

3D structure databases

ProteinModelPortaliO35454.
SMRiO35454. Positions 87-579, 780-862.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini606 – 66358CBS 1
Add
BLAST
Domaini808 – 86962CBS 2
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi156 – 1605Selectivity filter part_1 By similarity
Motifi198 – 2025Selectivity filter part_2 By similarity
Motifi488 – 4925Selectivity filter part_3 By similarity

Sequence similaritiesi

Contains 2 CBS domains.

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0038.
HOGENOMiHOG000231081.
HOVERGENiHBG050985.
KOiK05015.

Family and domain databases

Gene3Di1.10.3080.10. 2 hits.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002248. Cl_channel-6.
[Graphical view]
PfamiPF00571. CBS. 1 hit.
PF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01117. CLCHANNEL6.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
SUPFAMiSSF81340. SSF81340. 2 hits.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform ClC-6a (identifier: O35454-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAGCRGSVCC CCRWCCCCGE RESRTPEELT ILGETQEEED EILPRKDYES    50
LDYDRCINDP YLEVLETMDN KKGRRYEAVK WMVVFAIGVC TGLVGLFVDF 100
SVRLFTQLKF GVVQTSVEEC SQKGCLALSL LELLGFNLTF VFLASLLVLI 150
EPVAAGSGIP EIKCYLNGVK VPGIVRLRTL LCKVFGVLFS VSGGLFVGKE 200
GPMIHSGAVV GAGLPQFQSI SLRKIQFNFP YFRSDRDKRD FVSAGAAAGV 250
AAAFGAPIGG TLFSLEEGSS FWNQGLTWKV LFCSMSATFT LNFFRSGIQF 300
GSWGSFQLPG LLNFGEFKCS DSDKKCHLWT AMDLGFFVVM GVIGGLLGAT 350
FNCLNKRLAK YRMRNVHPKP KLVRVLESLL VSLVTTVVVF VASMVLGECR 400
QMSSTSQTGN GSFQLQVTSE DVNSTIKAFF CPNDTYNDMA TLFFNSQESA 450
ILQLFHQDGT FSPVTLALFF ILYFLLACWT FGTSVPSGLF VPSLLCGAAF 500
GRLVANVLKS YIGLGHLYSG TFALIGAAAF LGGVVRMTIS LTVILIESTN 550
EITYGLPIMV TLMVAKWTGD LFNKGIYDVH IGLRGVPLLE WETDVEMDKL 600
RASDIMEPNL TYVYPHTRIQ SLVSILRTTV HHAFPVVTEN RGNEKEFMKG 650
NQLISNNIKF KKSSILTRAG EQRKRGQSMK SYPSSELRNV CDEHVASEEP 700
AEKEDLLQQM LERRYTPYPN LYPDQSPSED WTMEERFRPL TFHGLVLRSQ 750
LVTLLVRGVC YSESQSSASQ PRLSYAEMAE DYPRYPDIHD LDLTLLNPRM 800
IVDVTPYMNP SPFTVSPNTH VSQVFNLFRT MGLRHLPVVN AVGEIVGIIT 850
RHNLTNEFLQ ARLRQHYQTL 870
Length:870
Mass (Da):96,980
Last modified:January 1, 1998 - v1
Checksum:i872C090069188749
GO
Isoform ClC-6c (identifier: O35454-2)

Sequence is not available
Length:
Mass (Da):

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF030106
, AF030101, AF030102, AF030103, AF030104, AF030105 Genomic DNA. Translation: AAC17702.1.
CCDSiCCDS18928.1. [O35454-1]
RefSeqiNP_036059.1. NM_011929.2. [O35454-1]
UniGeneiMm.89987.

Genome annotation databases

EnsembliENSMUST00000030879; ENSMUSP00000030879; ENSMUSG00000029016. [O35454-1]
GeneIDi26372.
KEGGimmu:26372.
UCSCiuc008vtr.1. mouse. [O35454-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF030106
, AF030101 , AF030102 , AF030103 , AF030104 , AF030105 Genomic DNA. Translation: AAC17702.1 .
CCDSi CCDS18928.1. [O35454-1 ]
RefSeqi NP_036059.1. NM_011929.2. [O35454-1 ]
UniGenei Mm.89987.

3D structure databases

ProteinModelPortali O35454.
SMRi O35454. Positions 87-579, 780-862.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O35454. 1 interaction.
MINTi MINT-4090929.

Chemistry

ChEMBLi CHEMBL2176794.

Protein family/group databases

TCDBi 2.A.49.3.4. the chloride carrier/channel (clc) family.

PTM databases

PhosphoSitei O35454.

Proteomic databases

PaxDbi O35454.
PRIDEi O35454.

Protocols and materials databases

DNASUi 26372.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000030879 ; ENSMUSP00000030879 ; ENSMUSG00000029016 . [O35454-1 ]
GeneIDi 26372.
KEGGi mmu:26372.
UCSCi uc008vtr.1. mouse. [O35454-1 ]

Organism-specific databases

CTDi 1185.
MGIi MGI:1347049. Clcn6.

Phylogenomic databases

eggNOGi COG0038.
HOGENOMi HOG000231081.
HOVERGENi HBG050985.
KOi K05015.

Enzyme and pathway databases

Reactomei REACT_196640. Stimuli-sensing channels.

Miscellaneous databases

NextBioi 304269.
PROi O35454.
SOURCEi Search...

Gene expression databases

ArrayExpressi O35454.
Bgeei O35454.
CleanExi MM_CLCN6.
Genevestigatori O35454.

Family and domain databases

Gene3Di 1.10.3080.10. 2 hits.
InterProi IPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002248. Cl_channel-6.
[Graphical view ]
Pfami PF00571. CBS. 1 hit.
PF00654. Voltage_CLC. 1 hit.
[Graphical view ]
PRINTSi PR00762. CLCHANNEL.
PR01117. CLCHANNEL6.
SMARTi SM00116. CBS. 2 hits.
[Graphical view ]
SUPFAMi SSF81340. SSF81340. 2 hits.
PROSITEi PS51371. CBS. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Kornak U., Boesl M.R., Jentsch T.J.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Alternative splicing of ClC-6 (a member of the ClC chloride-channel family) transcripts generates three truncated isoforms one of which, ClC-6c, is kidney-specific."
    Eggermont J., Buyse G., Voets T., Tytgat J., De Smedt H., Droogmans G., Nilius B.
    Biochem. J. 325:269-276(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
    Tissue: Chronic myeloid leukemia cell.
  3. Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.

Entry informationi

Entry nameiCLCN6_MOUSE
AccessioniPrimary (citable) accession number: O35454
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: September 3, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The presence of conserved gating glutamate residues is typical for family members that function as antiporters By similarity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi