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Protein

Chloride transport protein 6

Gene

Clcn6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Chloride transport protein, initially identified as voltage-gated chloride channel. The presence of the conserved gating glutamate residues suggests that is functions as antiporter.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei157ChlorideBy similarity1
Sitei200Mediates proton transfer from the outer aqueous phase to the interior of the protein; involved in linking H(+) and Cl(-) transportBy similarity1
Sitei267Mediates proton transfer from the protein to the inner aqueous phaseBy similarity1
Binding sitei490Chloride; via amide nitrogenBy similarity1
Binding sitei577ChlorideBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi631 – 633ATPBy similarity3
Nucleotide bindingi850 – 853ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Chloride, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Protein family/group databases

TCDBi2.A.49.3.4. the chloride carrier/channel (clc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride transport protein 6
Alternative name(s):
Chloride channel protein 6
Short name:
ClC-6
Gene namesi
Name:Clcn6
Synonyms:Clc6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1347049. Clcn6.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 80CytoplasmicBy similarityAdd BLAST80
Transmembranei81 – 113HelicalBy similarityAdd BLAST33
Transmembranei128 – 150HelicalBy similarityAdd BLAST23
Intramembranei159 – 166HelicalBy similarity8
Transmembranei176 – 194HelicalBy similarityAdd BLAST19
Transmembranei200 – 217HelicalBy similarityAdd BLAST18
Intramembranei241 – 253HelicalBy similarityAdd BLAST13
Intramembranei257 – 265HelicalBy similarity9
Transmembranei277 – 294HelicalBy similarityAdd BLAST18
Transmembranei335 – 364HelicalBy similarityAdd BLAST30
Transmembranei371 – 392HelicalBy similarityAdd BLAST22
Transmembranei463 – 482HelicalBy similarityAdd BLAST20
Transmembranei488 – 512HelicalBy similarityAdd BLAST25
Intramembranei520 – 534HelicalBy similarityAdd BLAST15
Intramembranei535 – 537Note=Loop between two helicesBy similarity3
Intramembranei538 – 549HelicalBy similarityAdd BLAST12
Intramembranei550 – 553Note=Loop between two helicesBy similarity4
Transmembranei554 – 572HelicalBy similarityAdd BLAST19
Topological domaini573 – 870CytoplasmicBy similarityAdd BLAST298

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

Pathology & Biotechi

Disruption phenotypei

Reduced pain sensitivity and moderate behavioral abnormalities, but have normal fertility and are generally not very different from wild-type.1 Publication

Chemistry databases

ChEMBLiCHEMBL2176794.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000944501 – 870Chloride transport protein 6Add BLAST870

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi410N-linked (GlcNAc...)Sequence analysis1
Glycosylationi423N-linked (GlcNAc...)Sequence analysis1
Glycosylationi433N-linked (GlcNAc...)Sequence analysis1
Modified residuei774PhosphoserineCombined sources1

Post-translational modificationi

N-glycosylated on several asparagine residues.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO35454.
PRIDEiO35454.

PTM databases

iPTMnetiO35454.
PhosphoSitePlusiO35454.
SwissPalmiO35454.

Expressioni

Tissue specificityi

Detected in whole brain and in hippocampus neurons (at protein level). Detected in brain, trigeminus, dorsal root ganglion, spinal cord, eye, kidney, testis, skeletal muscle, thymus and pancreas. Isoform ClC-6c is expressed only in kidney.2 Publications

Gene expression databases

BgeeiENSMUSG00000029016.
CleanExiMM_CLCN6.
ExpressionAtlasiO35454. baseline and differential.
GenevisibleiO35454. MM.

Interactioni

Protein-protein interaction databases

IntActiO35454. 1 interactor.
MINTiMINT-4090929.
STRINGi10090.ENSMUSP00000030879.

Structurei

3D structure databases

ProteinModelPortaliO35454.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini606 – 663CBS 1PROSITE-ProRule annotationAdd BLAST58
Domaini808 – 869CBS 2PROSITE-ProRule annotationAdd BLAST62

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi156 – 160Selectivity filter part_1By similarity5
Motifi198 – 202Selectivity filter part_2By similarity5
Motifi488 – 492Selectivity filter part_3By similarity5

Sequence similaritiesi

Contains 2 CBS domains.PROSITE-ProRule annotation

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0474. Eukaryota.
COG0038. LUCA.
GeneTreeiENSGT00760000119109.
HOGENOMiHOG000231081.
HOVERGENiHBG050985.
InParanoidiO35454.
KOiK05015.

Family and domain databases

Gene3Di1.10.3080.10. 2 hits.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002248. Cl_channel-6.
[Graphical view]
PfamiPF00571. CBS. 2 hits.
PF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01117. CLCHANNEL6.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
SUPFAMiSSF81340. SSF81340. 2 hits.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform ClC-6a (identifier: O35454-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGCRGSVCC CCRWCCCCGE RESRTPEELT ILGETQEEED EILPRKDYES
60 70 80 90 100
LDYDRCINDP YLEVLETMDN KKGRRYEAVK WMVVFAIGVC TGLVGLFVDF
110 120 130 140 150
SVRLFTQLKF GVVQTSVEEC SQKGCLALSL LELLGFNLTF VFLASLLVLI
160 170 180 190 200
EPVAAGSGIP EIKCYLNGVK VPGIVRLRTL LCKVFGVLFS VSGGLFVGKE
210 220 230 240 250
GPMIHSGAVV GAGLPQFQSI SLRKIQFNFP YFRSDRDKRD FVSAGAAAGV
260 270 280 290 300
AAAFGAPIGG TLFSLEEGSS FWNQGLTWKV LFCSMSATFT LNFFRSGIQF
310 320 330 340 350
GSWGSFQLPG LLNFGEFKCS DSDKKCHLWT AMDLGFFVVM GVIGGLLGAT
360 370 380 390 400
FNCLNKRLAK YRMRNVHPKP KLVRVLESLL VSLVTTVVVF VASMVLGECR
410 420 430 440 450
QMSSTSQTGN GSFQLQVTSE DVNSTIKAFF CPNDTYNDMA TLFFNSQESA
460 470 480 490 500
ILQLFHQDGT FSPVTLALFF ILYFLLACWT FGTSVPSGLF VPSLLCGAAF
510 520 530 540 550
GRLVANVLKS YIGLGHLYSG TFALIGAAAF LGGVVRMTIS LTVILIESTN
560 570 580 590 600
EITYGLPIMV TLMVAKWTGD LFNKGIYDVH IGLRGVPLLE WETDVEMDKL
610 620 630 640 650
RASDIMEPNL TYVYPHTRIQ SLVSILRTTV HHAFPVVTEN RGNEKEFMKG
660 670 680 690 700
NQLISNNIKF KKSSILTRAG EQRKRGQSMK SYPSSELRNV CDEHVASEEP
710 720 730 740 750
AEKEDLLQQM LERRYTPYPN LYPDQSPSED WTMEERFRPL TFHGLVLRSQ
760 770 780 790 800
LVTLLVRGVC YSESQSSASQ PRLSYAEMAE DYPRYPDIHD LDLTLLNPRM
810 820 830 840 850
IVDVTPYMNP SPFTVSPNTH VSQVFNLFRT MGLRHLPVVN AVGEIVGIIT
860 870
RHNLTNEFLQ ARLRQHYQTL
Length:870
Mass (Da):96,980
Last modified:January 1, 1998 - v1
Checksum:i872C090069188749
GO
Isoform ClC-6c (identifier: O35454-2)
Sequence is not available
Length:
Mass (Da):

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030106
, AF030101, AF030102, AF030103, AF030104, AF030105 Genomic DNA. Translation: AAC17702.1.
CCDSiCCDS18928.1. [O35454-1]
RefSeqiNP_036059.1. NM_011929.3. [O35454-1]
UniGeneiMm.89987.

Genome annotation databases

EnsembliENSMUST00000030879; ENSMUSP00000030879; ENSMUSG00000029016. [O35454-1]
GeneIDi26372.
KEGGimmu:26372.
UCSCiuc008vtr.1. mouse. [O35454-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030106
, AF030101, AF030102, AF030103, AF030104, AF030105 Genomic DNA. Translation: AAC17702.1.
CCDSiCCDS18928.1. [O35454-1]
RefSeqiNP_036059.1. NM_011929.3. [O35454-1]
UniGeneiMm.89987.

3D structure databases

ProteinModelPortaliO35454.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO35454. 1 interactor.
MINTiMINT-4090929.
STRINGi10090.ENSMUSP00000030879.

Chemistry databases

ChEMBLiCHEMBL2176794.

Protein family/group databases

TCDBi2.A.49.3.4. the chloride carrier/channel (clc) family.

PTM databases

iPTMnetiO35454.
PhosphoSitePlusiO35454.
SwissPalmiO35454.

Proteomic databases

PaxDbiO35454.
PRIDEiO35454.

Protocols and materials databases

DNASUi26372.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030879; ENSMUSP00000030879; ENSMUSG00000029016. [O35454-1]
GeneIDi26372.
KEGGimmu:26372.
UCSCiuc008vtr.1. mouse. [O35454-1]

Organism-specific databases

CTDi1185.
MGIiMGI:1347049. Clcn6.

Phylogenomic databases

eggNOGiKOG0474. Eukaryota.
COG0038. LUCA.
GeneTreeiENSGT00760000119109.
HOGENOMiHOG000231081.
HOVERGENiHBG050985.
InParanoidiO35454.
KOiK05015.

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Miscellaneous databases

PROiO35454.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029016.
CleanExiMM_CLCN6.
ExpressionAtlasiO35454. baseline and differential.
GenevisibleiO35454. MM.

Family and domain databases

Gene3Di1.10.3080.10. 2 hits.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002248. Cl_channel-6.
[Graphical view]
PfamiPF00571. CBS. 2 hits.
PF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01117. CLCHANNEL6.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
SUPFAMiSSF81340. SSF81340. 2 hits.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLCN6_MOUSE
AccessioniPrimary (citable) accession number: O35454
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The presence of conserved gating glutamate residues is typical for family members that function as antiporters (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.