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O35454 (CLCN6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chloride transport protein 6
Alternative name(s):
Chloride channel protein 6
Short name=ClC-6
Gene names
Name:Clcn6
Synonyms:Clc6
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length870 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Chloride transport protein, initially identified as voltage-gated chloride channel. The presence of the conserved gating glutamate residues suggests that is functions as antiporter. Ref.3

Subcellular location

Endosome membrane; Multi-pass membrane protein Ref.3.

Tissue specificity

Detected in whole brain and in hippocampus neurons (at protein level). Detected in brain, trigeminus, dorsal root ganglion, spinal cord, eye, kidney, testis, skeletal muscle, thymus and pancreas. Isoform ClC-6c is expressed only in kidney. Ref.2 Ref.3

Post-translational modification

N-glycosylated on several asparagine residues By similarity.

Disruption phenotype

Reduced pain sensitivity and moderate behavioral abnormalities, but have normal fertility and are generally not very different from wild-type. Ref.3

Miscellaneous

The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The presence of conserved gating glutamate residues is typical for family members that function as antiporters By similarity.

Sequence similarities

Belongs to the chloride channel (TC 2.A.49) family. ClC-6/CLCN6 subfamily. [View classification]

Contains 2 CBS domains.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Select]
Isoform ClC-6a (identifier: O35454-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform ClC-6c (identifier: O35454-2)

The sequence of this isoform is not available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 870870Chloride transport protein 6
PRO_0000094450

Regions

Topological domain1 – 8080Cytoplasmic By similarity
Transmembrane81 – 11333Helical; By similarity
Transmembrane128 – 15023Helical; By similarity
Intramembrane159 – 1668Helical; By similarity
Transmembrane176 – 19419Helical; By similarity
Transmembrane200 – 21718Helical; By similarity
Intramembrane241 – 25313Helical; By similarity
Intramembrane257 – 2659Helical; By similarity
Transmembrane277 – 29418Helical; By similarity
Transmembrane335 – 36430Helical; By similarity
Transmembrane371 – 39222Helical; By similarity
Transmembrane463 – 48220Helical; By similarity
Transmembrane488 – 51225Helical; By similarity
Intramembrane520 – 53415Helical; By similarity
Intramembrane535 – 5373Note=Loop between two helices; By similarity
Intramembrane538 – 54912Helical; By similarity
Intramembrane550 – 5534Note=Loop between two helices; By similarity
Transmembrane554 – 57219Helical; By similarity
Topological domain573 – 870298Cytoplasmic By similarity
Domain606 – 66358CBS 1
Domain808 – 86962CBS 2
Nucleotide binding631 – 6333ATP By similarity
Nucleotide binding850 – 8534ATP By similarity
Motif156 – 1605Selectivity filter part_1 By similarity
Motif198 – 2025Selectivity filter part_2 By similarity
Motif488 – 4925Selectivity filter part_3 By similarity

Sites

Binding site1571Chloride By similarity
Binding site4901Chloride; via amide nitrogen By similarity
Binding site5771Chloride By similarity
Site2001Mediates proton transfer from the outer aqueous phase to the interior of the protein; involved in linking H(+) and Cl(-) transport By similarity
Site2671Mediates proton transfer from the protein to the inner aqueous phase By similarity

Amino acid modifications

Glycosylation4101N-linked (GlcNAc...) Potential
Glycosylation4231N-linked (GlcNAc...) Potential
Glycosylation4331N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Isoform ClC-6a [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 872C090069188749

FASTA87096,980
        10         20         30         40         50         60 
MAGCRGSVCC CCRWCCCCGE RESRTPEELT ILGETQEEED EILPRKDYES LDYDRCINDP 

        70         80         90        100        110        120 
YLEVLETMDN KKGRRYEAVK WMVVFAIGVC TGLVGLFVDF SVRLFTQLKF GVVQTSVEEC 

       130        140        150        160        170        180 
SQKGCLALSL LELLGFNLTF VFLASLLVLI EPVAAGSGIP EIKCYLNGVK VPGIVRLRTL 

       190        200        210        220        230        240 
LCKVFGVLFS VSGGLFVGKE GPMIHSGAVV GAGLPQFQSI SLRKIQFNFP YFRSDRDKRD 

       250        260        270        280        290        300 
FVSAGAAAGV AAAFGAPIGG TLFSLEEGSS FWNQGLTWKV LFCSMSATFT LNFFRSGIQF 

       310        320        330        340        350        360 
GSWGSFQLPG LLNFGEFKCS DSDKKCHLWT AMDLGFFVVM GVIGGLLGAT FNCLNKRLAK 

       370        380        390        400        410        420 
YRMRNVHPKP KLVRVLESLL VSLVTTVVVF VASMVLGECR QMSSTSQTGN GSFQLQVTSE 

       430        440        450        460        470        480 
DVNSTIKAFF CPNDTYNDMA TLFFNSQESA ILQLFHQDGT FSPVTLALFF ILYFLLACWT 

       490        500        510        520        530        540 
FGTSVPSGLF VPSLLCGAAF GRLVANVLKS YIGLGHLYSG TFALIGAAAF LGGVVRMTIS 

       550        560        570        580        590        600 
LTVILIESTN EITYGLPIMV TLMVAKWTGD LFNKGIYDVH IGLRGVPLLE WETDVEMDKL 

       610        620        630        640        650        660 
RASDIMEPNL TYVYPHTRIQ SLVSILRTTV HHAFPVVTEN RGNEKEFMKG NQLISNNIKF 

       670        680        690        700        710        720 
KKSSILTRAG EQRKRGQSMK SYPSSELRNV CDEHVASEEP AEKEDLLQQM LERRYTPYPN 

       730        740        750        760        770        780 
LYPDQSPSED WTMEERFRPL TFHGLVLRSQ LVTLLVRGVC YSESQSSASQ PRLSYAEMAE 

       790        800        810        820        830        840 
DYPRYPDIHD LDLTLLNPRM IVDVTPYMNP SPFTVSPNTH VSQVFNLFRT MGLRHLPVVN 

       850        860        870 
AVGEIVGIIT RHNLTNEFLQ ARLRQHYQTL 

« Hide

Isoform ClC-6c (Sequence not available).

References

[1]Kornak U., Boesl M.R., Jentsch T.J.
Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Alternative splicing of ClC-6 (a member of the ClC chloride-channel family) transcripts generates three truncated isoforms one of which, ClC-6c, is kidney-specific."
Eggermont J., Buyse G., Voets T., Tytgat J., De Smedt H., Droogmans G., Nilius B.
Biochem. J. 325:269-276(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
Tissue: Chronic myeloid leukemia cell.
[3]"Lysosomal storage disease upon disruption of the neuronal chloride transport protein ClC-6."
Poet M., Kornak U., Schweizer M., Zdebik A.A., Scheel O., Hoelter S., Wurst W., Schmitt A., Fuhrmann J.C., Planells-Cases R., Mole S.E., Huebner C.A., Jentsch T.J.
Proc. Natl. Acad. Sci. U.S.A. 103:13854-13859(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF030106 expand/collapse EMBL AC list , AF030101, AF030102, AF030103, AF030104, AF030105 Genomic DNA. Translation: AAC17702.1.
RefSeqNP_036059.1. NM_011929.2.
UniGeneMm.89987.

3D structure databases

ProteinModelPortalO35454.
SMRO35454. Positions 78-583, 608-639, 780-862.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO35454. 1 interaction.
MINTMINT-4090929.

Chemistry

ChEMBLCHEMBL2176794.

Protein family/group databases

TCDB2.A.49.3.4. the chloride carrier/channel (clc) family.

PTM databases

PhosphoSiteO35454.

Proteomic databases

PaxDbO35454.
PRIDEO35454.

Protocols and materials databases

DNASU26372.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000030879; ENSMUSP00000030879; ENSMUSG00000029016. [O35454-1]
GeneID26372.
KEGGmmu:26372.
UCSCuc008vtr.1. mouse. [O35454-1]

Organism-specific databases

CTD1185.
MGIMGI:1347049. Clcn6.

Phylogenomic databases

eggNOGCOG0038.
HOGENOMHOG000231081.
HOVERGENHBG050985.
KOK05015.

Gene expression databases

ArrayExpressO35454.
BgeeO35454.
CleanExMM_CLCN6.
GenevestigatorO35454.

Family and domain databases

Gene3D1.10.3080.10. 2 hits.
InterProIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002248. Cl_channel-6.
[Graphical view]
PfamPF00571. CBS. 1 hit.
PF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSPR00762. CLCHANNEL.
PR01117. CLCHANNEL6.
SMARTSM00116. CBS. 2 hits.
[Graphical view]
SUPFAMSSF81340. SSF81340. 2 hits.
PROSITEPS51371. CBS. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio304269.
PROO35454.
SOURCESearch...

Entry information

Entry nameCLCN6_MOUSE
AccessionPrimary (citable) accession number: O35454
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot