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O35454

- CLCN6_MOUSE

UniProt

O35454 - CLCN6_MOUSE

Protein

Chloride transport protein 6

Gene

Clcn6

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Chloride transport protein, initially identified as voltage-gated chloride channel. The presence of the conserved gating glutamate residues suggests that is functions as antiporter.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei157 – 1571ChlorideBy similarity
    Sitei200 – 2001Mediates proton transfer from the outer aqueous phase to the interior of the protein; involved in linking H(+) and Cl(-) transportBy similarity
    Sitei267 – 2671Mediates proton transfer from the protein to the inner aqueous phaseBy similarity
    Binding sitei490 – 4901Chloride; via amide nitrogenBy similarity
    Binding sitei577 – 5771ChlorideBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi631 – 6333ATPBy similarity
    Nucleotide bindingi850 – 8534ATPBy similarity

    GO - Molecular functioni

    1. antiporter activity Source: UniProtKB-KW
    2. ATP binding Source: UniProtKB-KW
    3. voltage-gated chloride channel activity Source: InterPro

    GO - Biological processi

    1. response to mechanical stimulus Source: Ensembl

    Keywords - Biological processi

    Antiport, Ion transport, Transport

    Keywords - Ligandi

    ATP-binding, Chloride, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_196640. Stimuli-sensing channels.

    Protein family/group databases

    TCDBi2.A.49.3.4. the chloride carrier/channel (clc) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chloride transport protein 6
    Alternative name(s):
    Chloride channel protein 6
    Short name:
    ClC-6
    Gene namesi
    Name:Clcn6
    Synonyms:Clc6
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:1347049. Clcn6.

    Subcellular locationi

    Endosome membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. endosome membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. lysosomal membrane Source: Ensembl

    Keywords - Cellular componenti

    Endosome, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Reduced pain sensitivity and moderate behavioral abnormalities, but have normal fertility and are generally not very different from wild-type.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 870870Chloride transport protein 6PRO_0000094450Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi410 – 4101N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi423 – 4231N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi433 – 4331N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    N-glycosylated on several asparagine residues.By similarity

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiO35454.
    PRIDEiO35454.

    PTM databases

    PhosphoSiteiO35454.

    Expressioni

    Tissue specificityi

    Detected in whole brain and in hippocampus neurons (at protein level). Detected in brain, trigeminus, dorsal root ganglion, spinal cord, eye, kidney, testis, skeletal muscle, thymus and pancreas. Isoform ClC-6c is expressed only in kidney.2 Publications

    Gene expression databases

    ArrayExpressiO35454.
    BgeeiO35454.
    CleanExiMM_CLCN6.
    GenevestigatoriO35454.

    Interactioni

    Protein-protein interaction databases

    IntActiO35454. 1 interaction.
    MINTiMINT-4090929.

    Structurei

    3D structure databases

    ProteinModelPortaliO35454.
    SMRiO35454. Positions 87-579, 780-862.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 8080CytoplasmicBy similarityAdd
    BLAST
    Topological domaini573 – 870298CytoplasmicBy similarityAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei159 – 1668HelicalBy similarity
    Intramembranei241 – 25313HelicalBy similarityAdd
    BLAST
    Intramembranei257 – 2659HelicalBy similarity
    Intramembranei520 – 53415HelicalBy similarityAdd
    BLAST
    Intramembranei535 – 5373Note=Loop between two helicesBy similarity
    Intramembranei538 – 54912HelicalBy similarityAdd
    BLAST
    Intramembranei550 – 5534Note=Loop between two helicesBy similarity

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei81 – 11333HelicalBy similarityAdd
    BLAST
    Transmembranei128 – 15023HelicalBy similarityAdd
    BLAST
    Transmembranei176 – 19419HelicalBy similarityAdd
    BLAST
    Transmembranei200 – 21718HelicalBy similarityAdd
    BLAST
    Transmembranei277 – 29418HelicalBy similarityAdd
    BLAST
    Transmembranei335 – 36430HelicalBy similarityAdd
    BLAST
    Transmembranei371 – 39222HelicalBy similarityAdd
    BLAST
    Transmembranei463 – 48220HelicalBy similarityAdd
    BLAST
    Transmembranei488 – 51225HelicalBy similarityAdd
    BLAST
    Transmembranei554 – 57219HelicalBy similarityAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini606 – 66358CBS 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini808 – 86962CBS 2PROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi156 – 1605Selectivity filter part_1By similarity
    Motifi198 – 2025Selectivity filter part_2By similarity
    Motifi488 – 4925Selectivity filter part_3By similarity

    Sequence similaritiesi

    Contains 2 CBS domains.PROSITE-ProRule annotation

    Keywords - Domaini

    CBS domain, Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0038.
    HOGENOMiHOG000231081.
    HOVERGENiHBG050985.
    KOiK05015.

    Family and domain databases

    Gene3Di1.10.3080.10. 2 hits.
    InterProiIPR000644. CBS_dom.
    IPR014743. Cl-channel_core.
    IPR001807. Cl-channel_volt-gated.
    IPR002248. Cl_channel-6.
    [Graphical view]
    PfamiPF00571. CBS. 1 hit.
    PF00654. Voltage_CLC. 1 hit.
    [Graphical view]
    PRINTSiPR00762. CLCHANNEL.
    PR01117. CLCHANNEL6.
    SMARTiSM00116. CBS. 2 hits.
    [Graphical view]
    SUPFAMiSSF81340. SSF81340. 2 hits.
    PROSITEiPS51371. CBS. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform ClC-6a (identifier: O35454-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAGCRGSVCC CCRWCCCCGE RESRTPEELT ILGETQEEED EILPRKDYES    50
    LDYDRCINDP YLEVLETMDN KKGRRYEAVK WMVVFAIGVC TGLVGLFVDF 100
    SVRLFTQLKF GVVQTSVEEC SQKGCLALSL LELLGFNLTF VFLASLLVLI 150
    EPVAAGSGIP EIKCYLNGVK VPGIVRLRTL LCKVFGVLFS VSGGLFVGKE 200
    GPMIHSGAVV GAGLPQFQSI SLRKIQFNFP YFRSDRDKRD FVSAGAAAGV 250
    AAAFGAPIGG TLFSLEEGSS FWNQGLTWKV LFCSMSATFT LNFFRSGIQF 300
    GSWGSFQLPG LLNFGEFKCS DSDKKCHLWT AMDLGFFVVM GVIGGLLGAT 350
    FNCLNKRLAK YRMRNVHPKP KLVRVLESLL VSLVTTVVVF VASMVLGECR 400
    QMSSTSQTGN GSFQLQVTSE DVNSTIKAFF CPNDTYNDMA TLFFNSQESA 450
    ILQLFHQDGT FSPVTLALFF ILYFLLACWT FGTSVPSGLF VPSLLCGAAF 500
    GRLVANVLKS YIGLGHLYSG TFALIGAAAF LGGVVRMTIS LTVILIESTN 550
    EITYGLPIMV TLMVAKWTGD LFNKGIYDVH IGLRGVPLLE WETDVEMDKL 600
    RASDIMEPNL TYVYPHTRIQ SLVSILRTTV HHAFPVVTEN RGNEKEFMKG 650
    NQLISNNIKF KKSSILTRAG EQRKRGQSMK SYPSSELRNV CDEHVASEEP 700
    AEKEDLLQQM LERRYTPYPN LYPDQSPSED WTMEERFRPL TFHGLVLRSQ 750
    LVTLLVRGVC YSESQSSASQ PRLSYAEMAE DYPRYPDIHD LDLTLLNPRM 800
    IVDVTPYMNP SPFTVSPNTH VSQVFNLFRT MGLRHLPVVN AVGEIVGIIT 850
    RHNLTNEFLQ ARLRQHYQTL 870
    Length:870
    Mass (Da):96,980
    Last modified:January 1, 1998 - v1
    Checksum:i872C090069188749
    GO
    Isoform ClC-6c (identifier: O35454-2)

    Sequence is not available
    Length:
    Mass (Da):

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF030106
    , AF030101, AF030102, AF030103, AF030104, AF030105 Genomic DNA. Translation: AAC17702.1.
    CCDSiCCDS18928.1. [O35454-1]
    RefSeqiNP_036059.1. NM_011929.2. [O35454-1]
    UniGeneiMm.89987.

    Genome annotation databases

    EnsembliENSMUST00000030879; ENSMUSP00000030879; ENSMUSG00000029016. [O35454-1]
    GeneIDi26372.
    KEGGimmu:26372.
    UCSCiuc008vtr.1. mouse. [O35454-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF030106
    , AF030101 , AF030102 , AF030103 , AF030104 , AF030105 Genomic DNA. Translation: AAC17702.1 .
    CCDSi CCDS18928.1. [O35454-1 ]
    RefSeqi NP_036059.1. NM_011929.2. [O35454-1 ]
    UniGenei Mm.89987.

    3D structure databases

    ProteinModelPortali O35454.
    SMRi O35454. Positions 87-579, 780-862.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O35454. 1 interaction.
    MINTi MINT-4090929.

    Chemistry

    ChEMBLi CHEMBL2176794.

    Protein family/group databases

    TCDBi 2.A.49.3.4. the chloride carrier/channel (clc) family.

    PTM databases

    PhosphoSitei O35454.

    Proteomic databases

    PaxDbi O35454.
    PRIDEi O35454.

    Protocols and materials databases

    DNASUi 26372.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000030879 ; ENSMUSP00000030879 ; ENSMUSG00000029016 . [O35454-1 ]
    GeneIDi 26372.
    KEGGi mmu:26372.
    UCSCi uc008vtr.1. mouse. [O35454-1 ]

    Organism-specific databases

    CTDi 1185.
    MGIi MGI:1347049. Clcn6.

    Phylogenomic databases

    eggNOGi COG0038.
    HOGENOMi HOG000231081.
    HOVERGENi HBG050985.
    KOi K05015.

    Enzyme and pathway databases

    Reactomei REACT_196640. Stimuli-sensing channels.

    Miscellaneous databases

    NextBioi 304269.
    PROi O35454.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O35454.
    Bgeei O35454.
    CleanExi MM_CLCN6.
    Genevestigatori O35454.

    Family and domain databases

    Gene3Di 1.10.3080.10. 2 hits.
    InterProi IPR000644. CBS_dom.
    IPR014743. Cl-channel_core.
    IPR001807. Cl-channel_volt-gated.
    IPR002248. Cl_channel-6.
    [Graphical view ]
    Pfami PF00571. CBS. 1 hit.
    PF00654. Voltage_CLC. 1 hit.
    [Graphical view ]
    PRINTSi PR00762. CLCHANNEL.
    PR01117. CLCHANNEL6.
    SMARTi SM00116. CBS. 2 hits.
    [Graphical view ]
    SUPFAMi SSF81340. SSF81340. 2 hits.
    PROSITEi PS51371. CBS. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Kornak U., Boesl M.R., Jentsch T.J.
      Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Alternative splicing of ClC-6 (a member of the ClC chloride-channel family) transcripts generates three truncated isoforms one of which, ClC-6c, is kidney-specific."
      Eggermont J., Buyse G., Voets T., Tytgat J., De Smedt H., Droogmans G., Nilius B.
      Biochem. J. 325:269-276(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
      Tissue: Chronic myeloid leukemia cell.
    3. Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiCLCN6_MOUSE
    AccessioniPrimary (citable) accession number: O35454
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The presence of conserved gating glutamate residues is typical for family members that function as antiporters By similarity.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3