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O35453

- HEPS_MOUSE

UniProt

O35453 - HEPS_MOUSE

Protein

Serine protease hepsin

Gene

Hpn

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    May mediate the activating cleavage of HGF and MST1/HGFL. Plays a role in the proteolytic processing of ACE2 By similarity. Plays an essential role in cell growth and maintenance of cell morphology.By similarity

    Catalytic activityi

    Cleavage after basic amino-acid residues, with Arg strongly preferred to Lys.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei222 – 2221Charge relay systemBy similarity
    Active sitei276 – 2761Charge relay systemBy similarity
    Active sitei372 – 3721Charge relay systemBy similarity

    GO - Molecular functioni

    1. calcium-activated potassium channel activity Source: UniProtKB
    2. serine-type endopeptidase activity Source: InterPro
    3. serine-type exopeptidase activity Source: InterPro
    4. serine-type peptidase activity Source: UniProtKB

    GO - Biological processi

    1. basement membrane disassembly Source: UniProtKB
    2. cholesterol homeostasis Source: MGI
    3. cochlea morphogenesis Source: UniProtKB
    4. detection of mechanical stimulus involved in sensory perception of sound Source: UniProtKB
    5. epithelium development Source: UniProtKB
    6. negative regulation of alkaline phosphatase activity Source: MGI
    7. negative regulation of apoptotic process Source: UniProtKB
    8. negative regulation of epithelial cell proliferation Source: UniProtKB
    9. negative regulation of epithelial to mesenchymal transition Source: UniProtKB
    10. pilomotor reflex Source: UniProtKB
    11. positive regulation by host of viral transcription Source: UniProtKB
    12. positive regulation of cell growth Source: UniProtKB
    13. positive regulation of gene expression Source: UniProtKB
    14. positive regulation of hepatocyte proliferation Source: UniProtKB
    15. positive regulation of plasminogen activation Source: UniProtKB
    16. positive regulation of thyroid hormone generation Source: UniProtKB
    17. potassium ion transmembrane transport Source: UniProtKB
    18. proteolysis Source: UniProtKB
    19. regulation of cell shape Source: UniProtKB
    20. response to thyroid hormone Source: UniProtKB
    21. sensory perception of sound Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS01.224.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine protease hepsin (EC:3.4.21.106)
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Hpn
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1196620. Hpn.

    Subcellular locationi

    GO - Cellular componenti

    1. cell-cell junction Source: UniProtKB
    2. cell surface Source: UniProtKB
    3. cytoplasm Source: UniProtKB
    4. endoplasmic reticulum membrane Source: UniProtKB
    5. integral component of plasma membrane Source: UniProtKB
    6. neuronal cell body Source: UniProtKB
    7. nuclear membrane Source: Ensembl
    8. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 181181Serine protease hepsin non-catalytic chainSequence AnalysisPRO_0000027843Add
    BLAST
    Chaini182 – 436255Serine protease hepsin catalytic chainSequence AnalysisPRO_0000027844Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi96 ↔ 159PROSITE-ProRule annotation
    Disulfide bondi109 ↔ 169PROSITE-ProRule annotation
    Glycosylationi131 – 1311N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi138 ↔ 157PROSITE-ProRule annotation
    Disulfide bondi172 ↔ 296Interchain (between non-catalytic and catalytic chains)PROSITE-ProRule annotation
    Disulfide bondi207 ↔ 223PROSITE-ProRule annotation
    Disulfide bondi310 ↔ 378PROSITE-ProRule annotation
    Disulfide bondi341 ↔ 357PROSITE-ProRule annotation
    Disulfide bondi368 ↔ 400PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiO35453.
    PRIDEiO35453.

    PTM databases

    PhosphoSiteiO35453.

    Expressioni

    Gene expression databases

    ArrayExpressiO35453.
    BgeeiO35453.
    CleanExiMM_HPN.
    GenevestigatoriO35453.

    Structurei

    3D structure databases

    ProteinModelPortaliO35453.
    SMRiO35453. Positions 68-435.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3838CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini60 – 436377ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei39 – 5921Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini73 – 17098SRCRAdd
    BLAST
    Domaini182 – 424243Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation
    Contains 1 SRCR domain.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5640.
    GeneTreeiENSGT00730000110471.
    HOGENOMiHOG000251822.
    HOVERGENiHBG013304.
    KOiK08665.
    TreeFamiTF351678.

    Family and domain databases

    Gene3Di3.10.250.10. 1 hit.
    InterProiIPR015352. Hepsin-SRCR_dom.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR017448. SRCR-like_dom.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF09272. Hepsin-SRCR. 1 hit.
    PF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    ProDomiPD021735. Hepsin-SRCR. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00202. SR. 1 hit.
    SM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    SSF56487. SSF56487. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O35453-1) [UniParc]FASTAAdd to Basket

    Also known as: 1a

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAKEDEEPGA HRGGSTCSRP QPGKGGRTAA CCSRPKVAAL IVGTLLFLTG    50
    IGAASWAIVT ILLQSDQEPL YQVQLSPGDS RLAVFDKTEG TWRLLCSSRS 100
    NARVAGLGCE EMGFLRALAH SELDVRTAGA NGTSGFFCVD EGGLPLAQRL 150
    LDVISVCDCP RGRFLTATCQ DCGRRKLPVD RIVGGQDSSL GRWPWQVSLR 200
    YDGTHLCGGS LLSGDWVLTA AHCFPERNRV LSRWRVFAGA VARTSPHAVQ 250
    LGVQAVIYHG GYLPFRDPTI DENSNDIALV HLSSSLPLTE YIQPVCLPAA 300
    GQALVDGKVC TVTGWGNTQF YGQQAMVLQE ARVPIISNEV CNSPDFYGNQ 350
    IKPKMFCAGY PEGGIDACQG DSGGPFVCED SISGTSRWRL CGIVSWGTGC 400
    ALARKPGVYT KVTDFREWIF KAIKTHSEAS GMVTQP 436

    Note: Minor isoform.

    Length:436
    Mass (Da):46,821
    Last modified:July 27, 2011 - v3
    Checksum:iC0C71FFFF4D016F0
    GO
    Isoform 2 (identifier: O35453-2) [UniParc]FASTAAdd to Basket

    Also known as: 2a

    The sequence of this isoform differs from the canonical sequence as follows:
         25-44: Missing.

    Note: Major isoform.

    Show »
    Length:416
    Mass (Da):44,908
    Checksum:i3689A7277E186CF6
    GO

    Sequence cautioni

    The sequence BAB22289.2 differs from that shown. Reason: Frameshift at positions 155, 191 and 233.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti85 – 851F → L in AAB84221. (PubMed:9395459)Curated
    Sequence conflicti204 – 2041T → Y in BAB22289. (PubMed:16141072)Curated
    Sequence conflicti214 – 2141G → R in BAB22289. (PubMed:16141072)Curated
    Sequence conflicti228 – 2292NR → ET in BAB22289. (PubMed:16141072)Curated
    Sequence conflicti264 – 2641P → L in BAB22289. (PubMed:16141072)Curated
    Sequence conflicti281 – 2811H → N in BAB22289. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei25 – 4420Missing in isoform 2. 2 PublicationsVSP_007232Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF030065 mRNA. Translation: AAB84221.1.
    AK002694 mRNA. Translation: BAB22289.2. Frameshift.
    CH466593 Genomic DNA. Translation: EDL23952.1.
    BC138809 mRNA. Translation: AAI38810.1.
    BC145413 mRNA. Translation: AAI45414.1.
    CCDSiCCDS52188.1. [O35453-1]
    RefSeqiNP_001103722.1. NM_001110252.2. [O35453-1]
    NP_001263198.1. NM_001276269.1.
    NP_032307.2. NM_008281.4.
    XP_006539624.1. XM_006539561.1. [O35453-1]
    UniGeneiMm.19182.

    Genome annotation databases

    EnsembliENSMUST00000108102; ENSMUSP00000103737; ENSMUSG00000001249. [O35453-1]
    GeneIDi15451.
    KEGGimmu:15451.
    UCSCiuc009gid.2. mouse. [O35453-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF030065 mRNA. Translation: AAB84221.1 .
    AK002694 mRNA. Translation: BAB22289.2 . Frameshift.
    CH466593 Genomic DNA. Translation: EDL23952.1 .
    BC138809 mRNA. Translation: AAI38810.1 .
    BC145413 mRNA. Translation: AAI45414.1 .
    CCDSi CCDS52188.1. [O35453-1 ]
    RefSeqi NP_001103722.1. NM_001110252.2. [O35453-1 ]
    NP_001263198.1. NM_001276269.1.
    NP_032307.2. NM_008281.4.
    XP_006539624.1. XM_006539561.1. [O35453-1 ]
    UniGenei Mm.19182.

    3D structure databases

    ProteinModelPortali O35453.
    SMRi O35453. Positions 68-435.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S01.224.

    PTM databases

    PhosphoSitei O35453.

    Proteomic databases

    PaxDbi O35453.
    PRIDEi O35453.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000108102 ; ENSMUSP00000103737 ; ENSMUSG00000001249 . [O35453-1 ]
    GeneIDi 15451.
    KEGGi mmu:15451.
    UCSCi uc009gid.2. mouse. [O35453-1 ]

    Organism-specific databases

    CTDi 3249.
    MGIi MGI:1196620. Hpn.

    Phylogenomic databases

    eggNOGi COG5640.
    GeneTreei ENSGT00730000110471.
    HOGENOMi HOG000251822.
    HOVERGENi HBG013304.
    KOi K08665.
    TreeFami TF351678.

    Miscellaneous databases

    NextBioi 288256.
    PROi O35453.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O35453.
    Bgeei O35453.
    CleanExi MM_HPN.
    Genevestigatori O35453.

    Family and domain databases

    Gene3Di 3.10.250.10. 1 hit.
    InterProi IPR015352. Hepsin-SRCR_dom.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR017448. SRCR-like_dom.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF09272. Hepsin-SRCR. 1 hit.
    PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    ProDomi PD021735. Hepsin-SRCR. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00202. SR. 1 hit.
    SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    SSF56487. SSF56487. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and cloning of the membrane-associated serine protease, hepsin, from mouse preimplantation embryos."
      Vu T.-K.H., Liu R.W., Haaksma C., Tomasek J.J., Howard E.W.
      J. Biol. Chem. 272:31315-31320(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Tissue: Liver.
    2. "Complete nucleotide sequence, origin of isoform and functional characterization of the mouse hepsin gene."
      Kawamura S., Kurachi S., Deyashiki Y., Kurachi K.
      Eur. J. Biochem. 262:755-764(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Kidney.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Lung.

    Entry informationi

    Entry nameiHEPS_MOUSE
    AccessioniPrimary (citable) accession number: O35453
    Secondary accession number(s): B2RSC4, Q9CW97
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 123 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3