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Protein

E3 ubiquitin-protein ligase RNF5

Gene

Rnf5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-150 'leading to its proteasomal degradation; the ubiquitination occurrs in mitochondria after viral transfection and regulates antiviral responses (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri27 – 6842RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • cellular protein catabolic process Source: MGI
  • ERAD pathway Source: MGI
  • ER-associated misfolded protein catabolic process Source: UniProtKB
  • negative regulation of autophagy Source: MGI
  • protein destabilization Source: MGI
  • protein K48-linked ubiquitination Source: MGI
  • protein K63-linked ubiquitination Source: UniProtKB
  • protein ubiquitination Source: MGI
  • regulation of autophagosome assembly Source: MGI
  • response to bacterium Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF5 (EC:6.3.2.-)
Alternative name(s):
RING finger protein 5
Gene namesi
Name:Rnf5
Synonyms:Ng2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1860076. Rnf5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei118 – 13821HelicalSequence analysisAdd
BLAST
Transmembranei160 – 18021HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 180179E3 ubiquitin-protein ligase RNF5PRO_0000240394Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei84 – 841PhosphoserineBy similarity
Modified residuei94 – 941PhosphothreonineBy similarity
Modified residuei107 – 1071PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiO35445.
PaxDbiO35445.
PeptideAtlasiO35445.
PRIDEiO35445.

PTM databases

iPTMnetiO35445.
PhosphoSiteiO35445.

Expressioni

Gene expression databases

BgeeiO35445.
CleanExiMM_RNF5.
GenevisibleiO35445. MM.

Interactioni

Subunit structurei

Interacts with PXN (By similarity). Interacts with JKAMP. Interacts with TMEM173; the interaction of endogenous proteins is dependent on viral infection (By similarity).By similarity

Protein-protein interaction databases

BioGridi207597. 1 interaction.
MINTiMINT-1341225.
STRINGi10090.ENSMUSP00000015622.

Structurei

3D structure databases

ProteinModelPortaliO35445.
SMRiO35445. Positions 23-71.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri27 – 6842RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG0823. Eukaryota.
ENOG4111IHV. LUCA.
GeneTreeiENSGT00390000014107.
HOGENOMiHOG000238304.
HOVERGENiHBG054495.
InParanoidiO35445.
KOiK10666.
OMAiFQSFGDT.
OrthoDBiEOG7F7WBT.
PhylomeDBiO35445.
TreeFamiTF317334.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35445-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAEEEDGG PEGPNRERGG ASATFECNIC LETAREAVVS VCGHLYCWPC
60 70 80 90 100
LHQWLETRPD RQECPVCKAG ISREKVVPLY GRGSQKPQDP RLKTPPRPQG
110 120 130 140 150
QRPAPESRGG FQPFGDAGGF HFSFGVGAFP FGFFTTVFNA HEPFRRGAGV
160 170 180
DLGQGHPASS WQDSLFLFLA IFFFFWLLSI
Length:180
Mass (Da):19,837
Last modified:January 1, 1998 - v1
Checksum:i3C00A30201F590CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030001 Genomic DNA. Translation: AAB82008.1.
BC016449 mRNA. Translation: AAH16449.1.
CCDSiCCDS28650.1.
PIRiT09063.
RefSeqiNP_062276.1. NM_019403.3.
UniGeneiMm.274542.

Genome annotation databases

EnsembliENSMUST00000015622; ENSMUSP00000015622; ENSMUSG00000015478.
GeneIDi54197.
KEGGimmu:54197.
UCSCiuc008ccy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030001 Genomic DNA. Translation: AAB82008.1.
BC016449 mRNA. Translation: AAH16449.1.
CCDSiCCDS28650.1.
PIRiT09063.
RefSeqiNP_062276.1. NM_019403.3.
UniGeneiMm.274542.

3D structure databases

ProteinModelPortaliO35445.
SMRiO35445. Positions 23-71.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207597. 1 interaction.
MINTiMINT-1341225.
STRINGi10090.ENSMUSP00000015622.

PTM databases

iPTMnetiO35445.
PhosphoSiteiO35445.

Proteomic databases

MaxQBiO35445.
PaxDbiO35445.
PeptideAtlasiO35445.
PRIDEiO35445.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000015622; ENSMUSP00000015622; ENSMUSG00000015478.
GeneIDi54197.
KEGGimmu:54197.
UCSCiuc008ccy.1. mouse.

Organism-specific databases

CTDi6048.
MGIiMGI:1860076. Rnf5.

Phylogenomic databases

eggNOGiKOG0823. Eukaryota.
ENOG4111IHV. LUCA.
GeneTreeiENSGT00390000014107.
HOGENOMiHOG000238304.
HOVERGENiHBG054495.
InParanoidiO35445.
KOiK10666.
OMAiFQSFGDT.
OrthoDBiEOG7F7WBT.
PhylomeDBiO35445.
TreeFamiTF317334.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiO35445.
SOURCEiSearch...

Gene expression databases

BgeeiO35445.
CleanExiMM_RNF5.
GenevisibleiO35445. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse."
    Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D., Hood L.
    Genome Res. 13:2621-2636(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 129.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Salivary gland.
  3. "RNF5, a RING finger protein that regulates cell motility by targeting paxillin ubiquitination and altered localization."
    Didier C., Broday L., Bhoumik A., Israeli S., Takahashi S., Nakayama K., Thomas S.M., Turner C.E., Henderson S., Sabe H., Ronai Z.
    Mol. Cell. Biol. 23:5331-5345(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  4. "JAMP, a Jun N-terminal kinase 1 (JNK1)-associated membrane protein, regulates duration of JNK activity."
    Kadoya T., Khurana A., Tcherpakov M., Bromberg K.D., Didier C., Broday L., Asahara T., Bhoumik A., Ronai Z.
    Mol. Cell. Biol. 25:8619-8630(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH JKAMP.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiRNF5_MOUSE
AccessioniPrimary (citable) accession number: O35445
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: January 1, 1998
Last modified: July 6, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.