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Protein

Transcription factor 21

Gene

Tcf21

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in epithelial-mesenchymal interactions in kidney and lung morphogenesis that include epithelial differentiation and branching morphogenesis. May be involved in the organogenesis of the spleen and heart and in cardiac and coronary artery development. May function in the development and sex differentiation of gonad via transcriptional regulation of AD4BP/SF-1.1 Publication

GO - Molecular functioni

  • androgen receptor binding Source: BHF-UCL
  • bHLH transcription factor binding Source: MGI
  • E-box binding Source: BHF-UCL
  • histone deacetylase binding Source: BHF-UCL
  • protein dimerization activity Source: UniProtKB
  • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: UniProtKB
  • transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: UniProtKB

GO - Biological processi

  • branching involved in ureteric bud morphogenesis Source: MGI
  • branchiomeric skeletal muscle development Source: UniProtKB
  • bronchiole development Source: MGI
  • diaphragm development Source: UniProtKB
  • embryonic digestive tract morphogenesis Source: UniProtKB
  • epithelial cell differentiation Source: MGI
  • gland development Source: UniProtKB
  • glomerulus development Source: MGI
  • kidney development Source: MGI
  • lung alveolus development Source: MGI
  • lung morphogenesis Source: MGI
  • lung vasculature development Source: MGI
  • metanephric glomerular capillary formation Source: UniProtKB
  • metanephric mesenchymal cell differentiation Source: UniProtKB
  • morphogenesis of a branching structure Source: MGI
  • negative regulation of androgen receptor signaling pathway Source: BHF-UCL
  • negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • organ morphogenesis Source: MGI
  • palate development Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • regulation of histone deacetylation Source: BHF-UCL
  • reproductive structure development Source: MGI
  • respiratory system development Source: MGI
  • Sertoli cell differentiation Source: UniProtKB
  • sex determination Source: MGI
  • sex differentiation Source: MGI
  • spleen development Source: UniProtKB
  • transcription from RNA polymerase II promoter Source: GOC
  • ureteric bud development Source: MGI
  • vasculature development Source: MGI
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor 21
Short name:
TCF-21
Alternative name(s):
Capsulin
Epicardin
Podocyte-expressed 1
Short name:
Pod-1
Gene namesi
Name:Tcf21
Synonyms:Pod1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1202715. Tcf21.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 179179Transcription factor 21PRO_0000127465Add
BLAST

Proteomic databases

PaxDbiO35437.
PRIDEiO35437.

PTM databases

iPTMnetiO35437.
PhosphoSiteiO35437.

Expressioni

Tissue specificityi

Expressed at high levels in lung, kidney, gut, heart, ovary and podocytes (visceral glomerular epithelial cells). Also found in spleen, large intestine, uterus, bladder and testis.

Developmental stagei

Expressed during embryogenesis specifically in mesodermally-derived cells that surround the epithelium of the developing gastrointestinal, genitourinary, respiratory systems and in spiral septum of the heart and in epicardial precursor cells fated to form the coronary arteries. Expression pattern is sex- and stage-dependent during gonadogenesis. At 13.5 dpc expressed at higher levels in testis than ovary. In 3-month old adults expression drastically decreased in testis while it increased in ovary showing an opposite sex-dependent pattern in adults compared with fetuses. Expression in other organs was similar between the adults and the fetal stage.

Gene expression databases

BgeeiO35437.
CleanExiMM_TCF21.
GenevisibleiO35437. MM.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with TCF3 and binds the E box (5'-CANNTG-3').

GO - Molecular functioni

  • androgen receptor binding Source: BHF-UCL
  • bHLH transcription factor binding Source: MGI
  • histone deacetylase binding Source: BHF-UCL
  • protein dimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi204005. 4 interactions.
IntActiO35437. 1 interaction.
MINTiMINT-218623.
STRINGi10090.ENSMUSP00000053178.

Structurei

3D structure databases

ProteinModelPortaliO35437.
SMRiO35437. Positions 80-135.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini79 – 13153bHLHPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4029. Eukaryota.
ENOG411227D. LUCA.
GeneTreeiENSGT00710000106684.
HOGENOMiHOG000261670.
HOVERGENiHBG105298.
InParanoidiO35437.
OMAiNESTEEG.
OrthoDBiEOG7J181B.
PhylomeDBiO35437.
TreeFamiTF350742.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35437-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTGSLSDVE DLQEVEMLDC DSLKVDSNKE FGTSNESTEE GSNCENGSPQ
60 70 80 90 100
KGRGGLGKRR KAPTKKSPLS GVSQEGKQVQ RNAANARERA RMRVLSKAFS
110 120 130 140 150
RLKTTLPWVP PDTKLSKLDT LRLASSYIAH LRQILANDKY ENGYIHPVNL
160 170
TWPFMVAGKP ENDLKEVVTA NRLCGTTAS
Length:179
Mass (Da):19,681
Last modified:January 1, 1998 - v1
Checksum:i7BA3892818DB355F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF029753 mRNA. Translation: AAB84256.1.
AF047418 mRNA. Translation: AAC62531.1.
AF036945 mRNA. Translation: AAC23537.1.
AF035717 mRNA. Translation: AAC62513.1.
AB009453 mRNA. Translation: BAA23883.1.
AK011575 mRNA. Translation: BAB27709.1.
AK135423 mRNA. Translation: BAE22527.1.
AK157289 mRNA. Translation: BAE34032.1.
BC053525 mRNA. Translation: AAH53525.1.
CCDSiCCDS23732.1.
RefSeqiNP_035675.1. NM_011545.1.
UniGeneiMm.16497.

Genome annotation databases

EnsembliENSMUST00000049930; ENSMUSP00000053178; ENSMUSG00000045680.
GeneIDi21412.
KEGGimmu:21412.
UCSCiuc007eps.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF029753 mRNA. Translation: AAB84256.1.
AF047418 mRNA. Translation: AAC62531.1.
AF036945 mRNA. Translation: AAC23537.1.
AF035717 mRNA. Translation: AAC62513.1.
AB009453 mRNA. Translation: BAA23883.1.
AK011575 mRNA. Translation: BAB27709.1.
AK135423 mRNA. Translation: BAE22527.1.
AK157289 mRNA. Translation: BAE34032.1.
BC053525 mRNA. Translation: AAH53525.1.
CCDSiCCDS23732.1.
RefSeqiNP_035675.1. NM_011545.1.
UniGeneiMm.16497.

3D structure databases

ProteinModelPortaliO35437.
SMRiO35437. Positions 80-135.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204005. 4 interactions.
IntActiO35437. 1 interaction.
MINTiMINT-218623.
STRINGi10090.ENSMUSP00000053178.

PTM databases

iPTMnetiO35437.
PhosphoSiteiO35437.

Proteomic databases

PaxDbiO35437.
PRIDEiO35437.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049930; ENSMUSP00000053178; ENSMUSG00000045680.
GeneIDi21412.
KEGGimmu:21412.
UCSCiuc007eps.2. mouse.

Organism-specific databases

CTDi6943.
MGIiMGI:1202715. Tcf21.

Phylogenomic databases

eggNOGiKOG4029. Eukaryota.
ENOG411227D. LUCA.
GeneTreeiENSGT00710000106684.
HOGENOMiHOG000261670.
HOVERGENiHBG105298.
InParanoidiO35437.
OMAiNESTEEG.
OrthoDBiEOG7J181B.
PhylomeDBiO35437.
TreeFamiTF350742.

Miscellaneous databases

PROiO35437.
SOURCEiSearch...

Gene expression databases

BgeeiO35437.
CleanExiMM_TCF21.
GenevisibleiO35437. MM.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of capsulin, a basic helix-loop-helix factor expressed in progenitor cells of the pericardium and the coronary arteries."
    Hidai H., Bardales R., Goodwin R., Quertermous T., Quertermous E.E.
    Mech. Dev. 73:33-43(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Embryo.
  2. "Epicardin: a novel basic helix-loop-helix transcription factor gene expressed in epicardium, branchial arch myoblasts, and mesenchyme of developing lung, gut, kidney, and gonads."
    Robb L., Mifsud L., Hartley L., Biben C., Copeland N.G., Gilbert D.J., Jenkins N.A., Harvey R.P.
    Dev. Dyn. 213:105-113(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
    Tissue: Embryonic heart, Fibroblast and Uterus.
  3. "Capsulin: a novel bHLH transcription factor expressed in epicardial progenitors and mesenchyme of visceral organs."
    Lu J.-R., Richardson J.A., Olson E.N.
    Mech. Dev. 73:23-32(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Embryo.
  4. "The basic-helix-loop-helix protein pod1 is critically important for kidney and lung organogenesis."
    Quaggin S.E., Schwartz L., Cui S., Igarashi P., Deimling J., Post M., Rossant J.
    Development 126:5771-5783(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 129/Sv.
  5. "Pod-1/Capsulin shows a sex- and stage-dependent expression pattern in the mouse gonad development and represses expression of Ad4BP/SF-1."
    Tamura M., Kanno Y., Chuma S., Saito T., Nakatsuji N.
    Mech. Dev. 102:135-144(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  6. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Embryo, Muellerian duct and Spleen.
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon.
  8. "The basic helix-loop-helix transcription factor capsulin controls spleen organogenesis."
    Lu J.-R., Chang P., Richardson J.A., Gan L., Weiler H., Olson E.N.
    Proc. Natl. Acad. Sci. U.S.A. 97:9525-9530(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiTCF21_MOUSE
AccessioniPrimary (citable) accession number: O35437
Secondary accession number(s): Q3U023
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: January 1, 1998
Last modified: June 8, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.