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Protein

Dihydroorotate dehydrogenase (quinone), mitochondrial

Gene

Dhodh

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.

Catalytic activityi

(S)-dihydroorotate + a quinone = orotate + a quinol.

Cofactori

FMNBy similarityNote: Binds 1 FMN per subunit.By similarity

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes orotate from (S)-dihydroorotate (quinone route).
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydroorotate dehydrogenase (quinone), mitochondrial (Dhodh)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes orotate from (S)-dihydroorotate (quinone route), the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei99 – 991SubstrateBy similarity
Binding sitei119 – 1191FMNBy similarity
Binding sitei180 – 1801FMNBy similarity
Binding sitei211 – 2111FMNBy similarity
Active sitei214 – 2141NucleophileBy similarity
Binding sitei254 – 2541FMNBy similarity
Binding sitei282 – 2821FMN; via carbonyl oxygenBy similarity
Binding sitei305 – 3051FMN; via amide nitrogenBy similarity
Binding sitei334 – 3341FMN; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi95 – 995FMNBy similarity
Nucleotide bindingi355 – 3562FMNBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

Flavoprotein, FMN

Enzyme and pathway databases

ReactomeiR-MMU-500753. Pyrimidine biosynthesis.
UniPathwayiUPA00070; UER00946.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydroorotate dehydrogenase (quinone), mitochondrial (EC:1.3.5.2)
Short name:
DHOdehase
Alternative name(s):
Dihydroorotate oxidase
Gene namesi
Name:Dhodh
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1928378. Dhodh.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1010Mitochondrial matrixSequence analysis
Transmembranei11 – 3020HelicalSequence analysisAdd
BLAST
Topological domaini31 – 395365Mitochondrial intermembraneSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2991.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 395395Dihydroorotate dehydrogenase (quinone), mitochondrialPRO_0000029885Add
BLAST
Transit peptidei1 – 1010Mitochondrion; not cleavedBy similarity

Post-translational modificationi

The uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration.By similarity

Proteomic databases

EPDiO35435.
MaxQBiO35435.
PaxDbiO35435.
PeptideAtlasiO35435.
PRIDEiO35435.

PTM databases

PhosphoSiteiO35435.

Expressioni

Gene expression databases

BgeeiENSMUSG00000031730.
CleanExiMM_DHODH.
ExpressionAtlasiO35435. baseline and differential.
GenevisibleiO35435. MM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi208162. 1 interaction.
IntActiO35435. 1 interaction.
STRINGi10090.ENSMUSP00000070303.

Chemistry

BindingDBiO35435.

Structurei

3D structure databases

ProteinModelPortaliO35435.
SMRiO35435. Positions 35-395.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni144 – 1485Substrate bindingBy similarity
Regioni211 – 2166Substrate bindingBy similarity
Regioni283 – 2842Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1436. Eukaryota.
COG0167. LUCA.
GeneTreeiENSGT00500000044924.
HOGENOMiHOG000225103.
HOVERGENiHBG006898.
InParanoidiO35435.
KOiK00254.
OMAiERIKMGA.
OrthoDBiEOG091G07JK.
PhylomeDBiO35435.
TreeFamiTF105973.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00225. DHO_dh_type2. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR005720. Dihydroorotate_DH.
IPR005719. Dihydroorotate_DH_2.
IPR001295. Dihydroorotate_DH_CS.
[Graphical view]
PfamiPF01180. DHO_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01036. pyrD_sub2. 1 hit.
PROSITEiPS00911. DHODEHASE_1. 1 hit.
PS00912. DHODEHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35435-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAWRQLRKRA LDAAIILGGG GLLFTSYLTA TGDDHFYAEY LMPALQRLLD
60 70 80 90 100
PESAHRLAVR VISLGLLPRA TFQDSNMLEV RVLGHKFRNP VGIAAGFDKH
110 120 130 140 150
GEAVDGLYKL GFGFVEVGSV TPQPQEGNPR PRVFRLPEDQ AVINRYGFNS
160 170 180 190 200
HGLSAVEHRL RARQQKQTQL TTDGLPLGIN LGKNKTSVDA AADYVEGVRI
210 220 230 240 250
LGPLADYLVV NVSSPNTAGL RSLQGKTELR RLLSKVLQER DALKGPQKPA
260 270 280 290 300
VLVKIAPDLT AQDKEDIASV ARELGIDGLI ITNTTVSRPV GLQGALRSET
310 320 330 340 350
GGLSGKPLRD LSTQTIREMY ALTQGTIPII GVGGVSSGQD ALEKIQAGAS
360 370 380 390
LVQLYTALTF LGPPVVARVK RELEALLKER GFNTVTDAIG VDHRR
Length:395
Mass (Da):42,700
Last modified:October 1, 2000 - v2
Checksum:i84F2D93D0646E39D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF029667 mRNA. Translation: AAB82948.2.
BC019542 mRNA. Translation: AAH19542.1.
BC027829 mRNA. Translation: AAH27829.1.
BC045206 mRNA. Translation: AAH45206.1.
CCDSiCCDS40471.1.
RefSeqiNP_064430.1. NM_020046.3.
UniGeneiMm.23894.

Genome annotation databases

EnsembliENSMUST00000069058; ENSMUSP00000070303; ENSMUSG00000031730.
ENSMUST00000123605; ENSMUSP00000115934; ENSMUSG00000031730.
GeneIDi56749.
KEGGimmu:56749.
UCSCiuc009nip.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF029667 mRNA. Translation: AAB82948.2.
BC019542 mRNA. Translation: AAH19542.1.
BC027829 mRNA. Translation: AAH27829.1.
BC045206 mRNA. Translation: AAH45206.1.
CCDSiCCDS40471.1.
RefSeqiNP_064430.1. NM_020046.3.
UniGeneiMm.23894.

3D structure databases

ProteinModelPortaliO35435.
SMRiO35435. Positions 35-395.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208162. 1 interaction.
IntActiO35435. 1 interaction.
STRINGi10090.ENSMUSP00000070303.

Chemistry

BindingDBiO35435.
ChEMBLiCHEMBL2991.

PTM databases

PhosphoSiteiO35435.

Proteomic databases

EPDiO35435.
MaxQBiO35435.
PaxDbiO35435.
PeptideAtlasiO35435.
PRIDEiO35435.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000069058; ENSMUSP00000070303; ENSMUSG00000031730.
ENSMUST00000123605; ENSMUSP00000115934; ENSMUSG00000031730.
GeneIDi56749.
KEGGimmu:56749.
UCSCiuc009nip.1. mouse.

Organism-specific databases

CTDi1723.
MGIiMGI:1928378. Dhodh.

Phylogenomic databases

eggNOGiKOG1436. Eukaryota.
COG0167. LUCA.
GeneTreeiENSGT00500000044924.
HOGENOMiHOG000225103.
HOVERGENiHBG006898.
InParanoidiO35435.
KOiK00254.
OMAiERIKMGA.
OrthoDBiEOG091G07JK.
PhylomeDBiO35435.
TreeFamiTF105973.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00946.
ReactomeiR-MMU-500753. Pyrimidine biosynthesis.

Miscellaneous databases

PROiO35435.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031730.
CleanExiMM_DHODH.
ExpressionAtlasiO35435. baseline and differential.
GenevisibleiO35435. MM.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00225. DHO_dh_type2. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR005720. Dihydroorotate_DH.
IPR005719. Dihydroorotate_DH_2.
IPR001295. Dihydroorotate_DH_CS.
[Graphical view]
PfamiPF01180. DHO_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01036. pyrD_sub2. 1 hit.
PROSITEiPS00911. DHODEHASE_1. 1 hit.
PS00912. DHODEHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPYRD_MOUSE
AccessioniPrimary (citable) accession number: O35435
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.