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Protein

Amyloid beta A4 precursor protein-binding family A member 2

Gene

Apba2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Putative function in synaptic vesicle exocytosis by binding to STXBP1, an essential component of the synaptic vesicle exocytotic machinery. May modulate processing of the beta-amyloid precursor protein (APP) and hence formation of beta-APP.

GO - Molecular functioni

  • beta-amyloid binding Source: RGD
  • phosphatidylinositol-4,5-bisphosphate binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Amyloid beta A4 precursor protein-binding family A member 2
Alternative name(s):
Adapter protein X11beta
Neuron-specific X11L protein
Neuronal Munc18-1-interacting protein 2
Short name:
Mint-2
Gene namesi
Name:Apba2
Synonyms:Mint2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi620845. Apba2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: GOC
  • plasma membrane Source: RGD
  • synaptic vesicle Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 750750Amyloid beta A4 precursor protein-binding family A member 2PRO_0000064619Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei11 – 111PhosphoserineBy similarity
Modified residuei209 – 2091PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO35431.
PRIDEiO35431.

PTM databases

iPTMnetiO35431.
PhosphoSiteiO35431.

Expressioni

Tissue specificityi

Brain.

Gene expression databases

GenevisibleiO35431. RN.

Interactioni

Subunit structurei

Part of a multimeric complex containing STXBP1 and syntaxin-1. Binds to the cytoplasmic domain of amyloid protein beta, and to the nuclear factor NF-kappa-B/p65 via its PDZ domain. Interacts with the N-terminal domain of APBA2BP (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
GraspQ8R4T54EBI-2028211,EBI-7361884
Stxbp1P617653EBI-2028211,EBI-1029097

Protein-protein interaction databases

BioGridi249775. 6 interactions.
IntActiO35431. 3 interactions.
MINTiMINT-245233.
STRINGi10116.ENSRNOP00000022049.

Structurei

Secondary structure

1
750
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi365 – 3695Combined sources
Beta strandi371 – 38515Combined sources
Helixi391 – 41727Combined sources
Beta strandi426 – 4349Combined sources
Beta strandi436 – 4438Combined sources
Turni444 – 4463Combined sources
Beta strandi449 – 4546Combined sources
Helixi455 – 4573Combined sources
Beta strandi458 – 4636Combined sources
Beta strandi465 – 47410Combined sources
Beta strandi498 – 5069Combined sources
Helixi510 – 53122Combined sources
Turni536 – 5383Combined sources
Helixi541 – 56020Combined sources
Beta strandi568 – 5725Combined sources
Helixi606 – 6094Combined sources
Beta strandi619 – 6224Combined sources
Helixi635 – 6384Combined sources
Turni639 – 6413Combined sources
Beta strandi648 – 6525Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3SUZX-ray2.70A365-750[»]
3SV1X-ray3.30A/B/C365-552[»]
ProteinModelPortaliO35431.
SMRiO35431. Positions 365-534, 568-750.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini369 – 556188PIDPROSITE-ProRule annotationAdd
BLAST
Domaini569 – 65587PDZ 1PROSITE-ProRule annotationAdd
BLAST
Domaini660 – 73576PDZ 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni185 – 27187STXBP1-bindingAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi92 – 954Poly-Glu

Domaini

Composed of an N-terminal domain that binds STXBP1, a middle phosphotyrosine-binding domain (PID/PTB) that mediates binding with the cytoplasmic domain of the beta-amyloid precursor protein, and two C-terminal PDZ domains thought to attach proteins to the plasma membrane.

Sequence similaritiesi

Contains 2 PDZ (DHR) domains.PROSITE-ProRule annotation
Contains 1 PID domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3605. Eukaryota.
ENOG410XSBD. LUCA.
GeneTreeiENSGT00390000013578.
HOGENOMiHOG000033981.
HOVERGENiHBG050523.
InParanoidiO35431.
KOiK20055.
OMAiRHEGRPK.
OrthoDBiEOG78PV8H.
PhylomeDBiO35431.
TreeFamiTF315245.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
2.30.42.10. 2 hits.
InterProiIPR030529. Apba2.
IPR001478. PDZ.
IPR011993. PH_dom-like.
IPR006020. PTB/PI_dom.
[Graphical view]
PANTHERiPTHR12345:SF12. PTHR12345:SF12. 1 hit.
PfamiPF00595. PDZ. 2 hits.
PF00640. PID. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 2 hits.
SM00462. PTB. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50106. PDZ. 2 hits.
PS01179. PID. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35431-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHRKRQSTA SSMLDHRARP GPIPHDQEPE NEDTELPLES YVPTGLELGT
60 70 80 90 100
LRPDSPTPEE QECHNHSPDG DSSSDYVNNT SEEEDYDEGL PEEEEGVTYY
110 120 130 140 150
IRYCPEDDSY LEGMDCNGEE YLAHGAHPVD TDECQEAVED WTDSVGPHTH
160 170 180 190 200
SHGAENSQEY PDSHLPIPED DPTVLEVHDQ EEDGHYCPSK ESYQDYYPPE
210 220 230 240 250
TNGNTGGASP YRMRRGDGDL EEQEEDIDQI VAEIKMSLSM TSITSASEAS
260 270 280 290 300
PEHMPELDPG DSTEACSPSD TGRGPSRQEA RPKSLNLPPE VKHSGDPQRG
310 320 330 340 350
LKTKTRTPEE RPKWPQEQVC NGLEQPRKQQ RSDLNGPTDN NNIPETKKVA
360 370 380 390 400
SFPSFVAVPG PCEPEDLIDG IIFAANYLGS TQLLSERNPS KNIRMMQAQE
410 420 430 440 450
AVSRVKRMQK AAKIKKKANS EGDAQTLTEV DLFISTQRIK VLNADTQETM
460 470 480 490 500
MDHALRTISY IADIGNIVVL MARRRMPRSA SQDCIETTPG AQEGKKQYKM
510 520 530 540 550
ICHVFESEDA QLIAQSIGQA FSVAYQEFLR ANGINPEDLS QKEYSDIINT
560 570 580 590 600
QEMYNDDLIH FSNSENCKEL QLEKHKGEIL GVVVVESGWG SILPTVILAN
610 620 630 640 650
MMNGGPAARS GKLSIGDQIM SINGTSLVGL PLATCQGIIK GLKNQTQVKL
660 670 680 690 700
NIVSCPPVTT VLIKRPDLKY QLGFSVQNGI ICSLMRGGIA ERGGVRVGHR
710 720 730 740 750
IIEINGQSVV ATAHEKIVQA LSNSVGEIHM KTMPAAMFRL LTGQETPLYI
Length:750
Mass (Da):82,850
Last modified:January 1, 1998 - v1
Checksum:iC96842E9657169B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF029107 mRNA. Translation: AAC05305.1.
RefSeqiNP_113968.1. NM_031780.1.
UniGeneiRn.62687.

Genome annotation databases

EnsembliENSRNOT00000022049; ENSRNOP00000022049; ENSRNOG00000016358.
GeneIDi83610.
KEGGirno:83610.
UCSCiRGD:620845. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF029107 mRNA. Translation: AAC05305.1.
RefSeqiNP_113968.1. NM_031780.1.
UniGeneiRn.62687.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3SUZX-ray2.70A365-750[»]
3SV1X-ray3.30A/B/C365-552[»]
ProteinModelPortaliO35431.
SMRiO35431. Positions 365-534, 568-750.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249775. 6 interactions.
IntActiO35431. 3 interactions.
MINTiMINT-245233.
STRINGi10116.ENSRNOP00000022049.

PTM databases

iPTMnetiO35431.
PhosphoSiteiO35431.

Proteomic databases

PaxDbiO35431.
PRIDEiO35431.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022049; ENSRNOP00000022049; ENSRNOG00000016358.
GeneIDi83610.
KEGGirno:83610.
UCSCiRGD:620845. rat.

Organism-specific databases

CTDi321.
RGDi620845. Apba2.

Phylogenomic databases

eggNOGiKOG3605. Eukaryota.
ENOG410XSBD. LUCA.
GeneTreeiENSGT00390000013578.
HOGENOMiHOG000033981.
HOVERGENiHBG050523.
InParanoidiO35431.
KOiK20055.
OMAiRHEGRPK.
OrthoDBiEOG78PV8H.
PhylomeDBiO35431.
TreeFamiTF315245.

Miscellaneous databases

PROiO35431.

Gene expression databases

GenevisibleiO35431. RN.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
2.30.42.10. 2 hits.
InterProiIPR030529. Apba2.
IPR001478. PDZ.
IPR011993. PH_dom-like.
IPR006020. PTB/PI_dom.
[Graphical view]
PANTHERiPTHR12345:SF12. PTHR12345:SF12. 1 hit.
PfamiPF00595. PDZ. 2 hits.
PF00640. PID. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 2 hits.
SM00462. PTB. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50106. PDZ. 2 hits.
PS01179. PID. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Mints, Munc18-interacting proteins in synaptic vesicle exocytosis."
    Okamoto M., Suedhof T.C.
    J. Biol. Chem. 272:31459-31464(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiAPBA2_RAT
AccessioniPrimary (citable) accession number: O35431
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: July 6, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.