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Protein

Amyloid beta A4 precursor protein-binding family A member 1

Gene

Apba1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Putative function in synaptic vesicle exocytosis by binding to Munc18-1, an essential component of the synaptic vesicle exocytotic machinery. May modulate processing of the beta-amyloid precursor protein (APP) and hence formation of beta-AAP.

GO - Molecular functioni

  • beta-amyloid binding Source: RGD
  • PDZ domain binding Source: RGD
  • phosphatidylinositol-4,5-bisphosphate binding Source: RGD
  • protein complex binding Source: RGD

GO - Biological processi

  • protein complex assembly Source: InterPro
  • protein transport Source: UniProtKB-KW
  • synaptic vesicle exocytosis Source: RGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-6794361. Interactions of neurexins and neuroligins at synapses.

Names & Taxonomyi

Protein namesi
Recommended name:
Amyloid beta A4 precursor protein-binding family A member 1
Alternative name(s):
Adapter protein X11alpha
Neuron-specific X11 protein
Neuronal Munc18-1-interacting protein 1
Short name:
Mint-1
Gene namesi
Name:Apba1
Synonyms:Mint1, X11
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620844. Apba1.

Subcellular locationi

Isoform 2 :

GO - Cellular componenti

  • dendritic spine Source: CACAO
  • Golgi apparatus Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB-SubCell
  • perinuclear region of cytoplasm Source: UniProtKB-SubCell
  • plasma membrane Source: RGD
  • protein complex Source: RGD
  • synaptic vesicle Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi633Y → E: Enhanced APP binding and beta-amyloid production. 1 Publication1
Mutagenesisi633Y → F: No change in APP binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000646151 – 839Amyloid beta A4 precursor protein-binding family A member 1Add BLAST839

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei79PhosphoserineBy similarity1
Modified residuei243PhosphoserineCombined sources1
Modified residuei247PhosphoserineCombined sources1
Modified residuei249PhosphoserineCombined sources1
Modified residuei264PhosphoserineCombined sources1
Modified residuei281PhosphoserineBy similarity1
Modified residuei286PhosphoserineCombined sources1
Modified residuei306PhosphothreonineBy similarity1
Modified residuei314PhosphoserineCombined sources1
Modified residuei369PhosphoserineBy similarity1
Modified residuei372PhosphothreonineBy similarity1
Modified residuei403PhosphoserineBy similarity1
Modified residuei405PhosphoserineBy similarity1
Modified residuei410PhosphoserineBy similarity1
Modified residuei570PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO35430.
PeptideAtlasiO35430.
PRIDEiO35430.

PTM databases

iPTMnetiO35430.
PhosphoSitePlusiO35430.

Expressioni

Tissue specificityi

Brain. Detected in the cerebellum, hippocampus, olfactory system, piriform and entorhinal cortex, supraoptic nucleus of the hypothalamus, substantia nigra, and other mesencephalic areas.

Gene expression databases

BgeeiENSRNOG00000014928.

Interactioni

Subunit structurei

Part of a multimeric complex containing Munc18-1 and syntaxin-1. Also part of the brain-specific heterotrimeric complex LIN-10/X11-alpha, LIN-2/CASK, and LIN7. Binds to the cytoplasmic domain of amyloid protein (APP) (By similarity). Interacts (via PDZ 1 and 2 domains) with FSPB (By similarity). Isoform 2 interacts (via its truncated PID domain) with active, GTP-bound RAB6A and RAB6B (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Stx1aP328513EBI-704760,EBI-539720
Stxbp1P617655EBI-704760,EBI-1029097

GO - Molecular functioni

  • PDZ domain binding Source: RGD
  • protein complex binding Source: RGD

Protein-protein interaction databases

BioGridi249774. 7 interactors.
IntActiO35430. 3 interactors.
MINTiMINT-102360.
STRINGi10116.ENSRNOP00000020123.

Chemistry databases

BindingDBiO35430.

Structurei

Secondary structure

1839
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi460 – 477Combined sources18
Helixi481 – 493Combined sources13
Beta strandi517 – 532Combined sources16
Turni533 – 535Combined sources3
Beta strandi538 – 543Combined sources6
Helixi544 – 546Combined sources3
Beta strandi547 – 553Combined sources7
Beta strandi556 – 563Combined sources8
Beta strandi587 – 595Combined sources9
Helixi599 – 614Combined sources16
Helixi615 – 617Combined sources3
Helixi625 – 627Combined sources3
Helixi630 – 639Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4DBBX-ray1.90A453-643[»]
ProteinModelPortaliO35430.
SMRiO35430.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini459 – 645PIDPROSITE-ProRule annotationAdd BLAST187
Domaini658 – 744PDZ 1PROSITE-ProRule annotationAdd BLAST87
Domaini749 – 824PDZ 2PROSITE-ProRule annotationAdd BLAST76

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni227 – 315Munc-18-1 bindingAdd BLAST89
Regioni375 – 438LIN-2/CASK bindingAdd BLAST64
Regioni628 – 643Autoinhibitory helix linkerAdd BLAST16

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi40 – 65Pro-richAdd BLAST26
Compositional biasi67 – 71Poly-Glu5
Compositional biasi295 – 331Pro-richAdd BLAST37
Compositional biasi370 – 454Pro-richAdd BLAST85

Domaini

Composed of an N-terminal domain that binds Munc18-1 and LIN-2/CASK, a middle phosphotyrosine-binding domain (PID/PTB) that mediates binding with the cytoplasmic domain of the beta-amyloid precursor protein, and two C-terminal PDZ domains thought to attach proteins to the plasma membrane.By similarity
The autoinhibitory helix linker occludes the APP binding site.
The PID domain, truncated by 11 amino acids, as observed in isoform 2, but not full-length, mediates the interaction with RAB6A.By similarity

Sequence similaritiesi

Contains 2 PDZ (DHR) domains.PROSITE-ProRule annotation
Contains 1 PID domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3605. Eukaryota.
ENOG410XSBD. LUCA.
HOGENOMiHOG000033981.
HOVERGENiHBG050523.
InParanoidiO35430.
KOiK04531.
PhylomeDBiO35430.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
2.30.42.10. 2 hits.
InterProiIPR030530. Apba1.
IPR001478. PDZ.
IPR011993. PH_dom-like.
IPR006020. PTB/PI_dom.
[Graphical view]
PANTHERiPTHR12345:SF14. PTHR12345:SF14. 1 hit.
PfamiPF00595. PDZ. 2 hits.
PF00640. PID. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 2 hits.
SM00462. PTB. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50106. PDZ. 2 hits.
PS01179. PID. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O35430-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNHLEGSAEV EVADEAPGGE VNESVEADLE HPEVEEEQQP SPPPPAGHAP
60 70 80 90 100
EDHRAHPAPP PPPPPQEEEE ERGECLARSA STESGFHNHT DTAEGDVLAA
110 120 130 140 150
ARDGYEAERA QDADDESAYA VQYRPEAEEY TEQAEAEHAE AAQRRALPNH
160 170 180 190 200
LHFHSLEHEE AMNAAYSGYV YTHRLFHRAE DEPYAEPYAD YGGLQEHVYE
210 220 230 240 250
EIGDAPELEA RDGLRLYERE RDEAAAYRQE ALGARLHHYD ERSDGESDSP
260 270 280 290 300
EKEAEFAPYP RMDSYEQEED IDQIVAEVKQ SMSSQSLDKA AEDMPEAEQD
310 320 330 340 350
LERAPTPGGG HPDSPGLPAP AGQQQRVVGT PGGSEVGQRY SKEKRDAISL
360 370 380 390 400
AIKDIKEAIE EVKTRTIRSP YTPDEPKEPI WVMRQDISPT RDCDDQRPVD
410 420 430 440 450
GDSPSPGSSS PLGAESSITP LHPGDPTEAS TNKESRKSLA SFPTYVEVPG
460 470 480 490 500
PCDPEDLIDG IIFAANYLGS TQLLSDKTPS KNVRMMQAQE AVSRIKTAQK
510 520 530 540 550
LAKSRKKAPE GESQPMTEVD LFISTQRIKV LNADTQEPMM DHPLRTISYI
560 570 580 590 600
ADIGNIVVLM ARRRMPRSNS QENVEASHPS QDAKRQYKMI CHVFESEDAQ
610 620 630 640 650
LIAQSIGQAF SVAYQEFLRA NGINPEDLSQ KEYSDLLNTQ DMYNDDLIHF
660 670 680 690 700
SKSENCKDVF IEKQKGEILG VVIVESGWGS ILPTVIIANM MHGGPAEKSG
710 720 730 740 750
KLNIGDQIMS INGTSLVGLP LSTCQSIIKG LKNQSRVKLN IVRCPPVTTV
760 770 780 790 800
LIRRPDLRYQ LGFSVQNGII CSLMRGGIAE RGGVRVGHRI IEINGQSVVA
810 820 830
TPHEKIVHIL SNAVGEIHMK TMPAAMYRLL TAQEQPVYI
Length:839
Mass (Da):92,654
Last modified:January 1, 1998 - v1
Checksum:i57F6EEA458376CAD
GO
Isoform 2 (identifier: O35430-2) [UniParc]FASTAAdd to basket
Also known as: Mint1_826

The sequence of this isoform differs from the canonical sequence as follows:
     497-507: Missing.

Note: This isoform interacts with RAB6 GTPases.By similarity
Show »
Length:828
Mass (Da):91,413
Checksum:iFE72D6DE098CFC27
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_053520497 – 507Missing in isoform 2. CuratedAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF029105 mRNA. Translation: AAC05303.1.
RefSeqiNP_113967.1. NM_031779.2. [O35430-1]
UniGeneiRn.54819.

Genome annotation databases

GeneIDi83589.
KEGGirno:83589.
UCSCiRGD:620844. rat. [O35430-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF029105 mRNA. Translation: AAC05303.1.
RefSeqiNP_113967.1. NM_031779.2. [O35430-1]
UniGeneiRn.54819.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4DBBX-ray1.90A453-643[»]
ProteinModelPortaliO35430.
SMRiO35430.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249774. 7 interactors.
IntActiO35430. 3 interactors.
MINTiMINT-102360.
STRINGi10116.ENSRNOP00000020123.

Chemistry databases

BindingDBiO35430.

PTM databases

iPTMnetiO35430.
PhosphoSitePlusiO35430.

Proteomic databases

PaxDbiO35430.
PeptideAtlasiO35430.
PRIDEiO35430.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi83589.
KEGGirno:83589.
UCSCiRGD:620844. rat. [O35430-1]

Organism-specific databases

CTDi320.
RGDi620844. Apba1.

Phylogenomic databases

eggNOGiKOG3605. Eukaryota.
ENOG410XSBD. LUCA.
HOGENOMiHOG000033981.
HOVERGENiHBG050523.
InParanoidiO35430.
KOiK04531.
PhylomeDBiO35430.

Enzyme and pathway databases

ReactomeiR-RNO-6794361. Interactions of neurexins and neuroligins at synapses.

Miscellaneous databases

PROiO35430.

Gene expression databases

BgeeiENSRNOG00000014928.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
2.30.42.10. 2 hits.
InterProiIPR030530. Apba1.
IPR001478. PDZ.
IPR011993. PH_dom-like.
IPR006020. PTB/PI_dom.
[Graphical view]
PANTHERiPTHR12345:SF14. PTHR12345:SF14. 1 hit.
PfamiPF00595. PDZ. 2 hits.
PF00640. PID. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 2 hits.
SM00462. PTB. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50106. PDZ. 2 hits.
PS01179. PID. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAPBA1_RAT
AccessioniPrimary (citable) accession number: O35430
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.