O35426 (XBP1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
X-box-binding protein 1
Short name=XBP-1

Alternative name(s):
Tax-responsive element-binding protein 5 homolog

Gene names

Name:	Xbp1
Synonyms:	Treb5

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N3[AT]T-3', and also to some TPA response elements (TRE) By similarity. Isoform 2 is involved in controlling the production of interleukin-6. Ref.6 Ref.7
Subcellular location	Nucleus.
Induction	Up-regulated in response to endoplasmic reticulum stress. Induced by interleukin-4 in plasma cell differentiation. Ref.2 Ref.7
Sequence similarities	Belongs to the bZIP family. Contains 1 bZIP (basic-leucine zipper) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to antibiotic Inferred from electronic annotation. Source: Ensembl endoplasmic reticulum unfolded protein response Inferred from electronic annotation. Source: Ensembl epithelial cell maturation Inferred from mutant phenotype PubMed 16362047. Source: MGI epithelial cell maturation involved in salivary gland development Inferred from mutant phenotype PubMed 16362047. Source: MGI exocrine pancreas development Inferred from mutant phenotype PubMed 16362047. Source: MGI glucose homeostasis Inferred from direct assay PubMed 21317886. Source: MGI positive regulation of endoplasmic reticulum unfolded protein response Inferred from electronic annotation. Source: Ensembl positive regulation of proteasomal protein catabolic process Inferred from direct assay PubMed 21317886. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 23152784. Source: MGI response to drug Inferred from electronic annotation. Source: Ensembl response to electrical stimulus Inferred from electronic annotation. Source: Ensembl serotonin secretion, neurotransmission Inferred from electronic annotation. Source: Ensembl
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA polymerase II transcription factor binding Inferred from physical interaction PubMed 21317886. Source: MGI sequence-specific DNA binding Inferred from electronic annotation. Source: Ensembl sequence-specific DNA binding RNA polymerase II transcription factor activity Inferred from direct assay PubMed 23152784. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O35426-1)

Also known as: XBP-1U;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O35426-2)

Also known as: XBP-1S;

The sequence of this isoform differs from the canonical sequence as follows:
     162-267: LRLCAPLQQV...FVLTMYTPSL → GAGPVVTSPE...NELFPQLISV

Note: Potent transcriptional activator. Induced by ERN1 in response to endoplasmic reticulum stress. ERN1 cleaves a 26-bp fragment causing a frameshift of the mRNA transcript.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	X-box-binding protein 1		PRO_0000076544
Regions
	Domain	63 – 126	64	bZIP
	Region	65 – 87	23	Basic motif By similarity
	Region	91 – 126	36	Leucine-zipper By similarity
Natural variations
	Alternative sequence	162 – 267	106	LRLCA…YTPSL → GAGPVVTSPEHLPMDSDTVA SSDSESDILLGILDKLDPVM FFKCPSPESASLEELPEVYP EGPSSLPASLSLSVGTSSAK LEAINELIRFDHVYTKPLVL EIPSETESQTNVVVKIEEAP LSSSEEDHPEFIVSVKKEPL EDDFIPELGISNLLSSSHCL RPPSCLLDAHSDCGYEGSPS PFSDMSSPLGTDHSWEDTFA NELFPQLISV in isoform 2.		VSP_012937
Experimental info
	Sequence conflict	27	1	Missing in BAB13793. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (XBP-1U) [UniParc]. Last modified June 1, 2001. Version 2. Checksum: 15009E684F6D426F	FASTA	267	29,619
	10 20 30 40 50 60 MVVVAAAPSA ATAAPKVLLL SGQPASGGRA LPLMVPGPRA AGSEASGTPQ ARKRQRLTHL 70 80 90 100 110 120 SPEEKALRRK LKNRVAAQTA RDRKKARMSE LEQQVVDLEE ENHKLQLENQ LLREKTHGLV 130 140 150 160 170 180 VENQELRTRL GMDTLDPDEV PEVEAKGSGV RLVAGSAESA ALRLCAPLQQ VQAQLSPPQN 190 200 210 220 230 240 IFPWTLTLLP LQILSLISFW AFWTSWTLSC FSNVLPQSLL VWRNSQRSTQ KDLVPYQPPF 250 260 LCQWGPHQPS WKPLMNSFVL TMYTPSL
	Isoform 2 (XBP-1S) [UniParc]. Checksum: 6F67EC346DBF3AC8	FASTA	371	39,888
	10 20 30 40 50 60 MVVVAAAPSA ATAAPKVLLL SGQPASGGRA LPLMVPGPRA AGSEASGTPQ ARKRQRLTHL 70 80 90 100 110 120 SPEEKALRRK LKNRVAAQTA RDRKKARMSE LEQQVVDLEE ENHKLQLENQ LLREKTHGLV 130 140 150 160 170 180 VENQELRTRL GMDTLDPDEV PEVEAKGSGV RLVAGSAESA AGAGPVVTSP EHLPMDSDTV 190 200 210 220 230 240 ASSDSESDIL LGILDKLDPV MFFKCPSPES ASLEELPEVY PEGPSSLPAS LSLSVGTSSA 250 260 270 280 290 300 KLEAINELIR FDHVYTKPLV LEIPSETESQ TNVVVKIEEA PLSSSEEDHP EFIVSVKKEP 310 320 330 340 350 360 LEDDFIPELG ISNLLSSSHC LRPPSCLLDA HSDCGYEGSP SPFSDMSSPL GTDHSWEDTF 370 ANELFPQLIS V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of the gene encoding mouse tax-responsive element-binding protein (TREB) 5." Masaki T., Noguchi H., Kobayashi M., Yoshida M., Takamatsu K. DNA Res. 7:187-193(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA." Calfon M., Zeng H., Urano F., Till J.H., Hubbard S.R., Harding H.P., Clark S.G., Ron D. Nature 415:92-96(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), INDUCTION. Strain: 129/SvEv.
[3]	"Sequence analysis of murine XBP-1." Lee C.M., Reddy E.P. Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Mammary tumor.
[6]	"XBP-1 regulates a subset of endoplasmic reticulum resident chaperone genes in the unfolded protein response." Lee A.H., Iwakoshi N.N., Glimcher L.H. Mol. Cell. Biol. 23:7448-7459(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Plasma cell differentiation and the unfolded protein response intersect at the transcription factor XBP-1." Iwakoshi N.N., Lee A.-H., Vallabhajosyula P., Otipoby K.L., Rajewsky K., Glimcher L.H. Nat. Immunol. 4:321-329(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB036745 Genomic DNA. Translation: BAB13793.1. AF443192 mRNA. Translation: AAL60202.1. AF027963 mRNA. Translation: AAB81862.2. AL662876 Genomic DNA. Translation: CAI24935.1. BC008153 mRNA. Translation: AAH08153.1. BC029197 mRNA. Translation: AAH29197.1.
PIR	JC7300.
RefSeq	NP_001258659.1. NM_001271730.1. NP_038870.2. NM_013842.3.
UniGene	Mm.469937.
3D structure databases
ProteinModelPortal	O35426.
SMR	O35426. Positions 64-126.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	204589. 1 interaction.
PTM databases
PhosphoSite	O35426.
Proteomic databases
PRIDE	O35426.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000063084; ENSMUSP00000054852; ENSMUSG00000020484. [O35426-1]
GeneID	22433.
KEGG	mmu:22433.
UCSC	uc007hwm.1. mouse. [O35426-1]
Organism-specific databases
CTD	7494.
MGI	MGI:98970. Xbp1.
Phylogenomic databases
eggNOG	NOG285368.
GeneTree	ENSGT00390000017751.
HOGENOM	HOG000007671.
HOVERGEN	HBG061457.
KO	K09027.
OMA	WKPLMNS.
OrthoDB	EOG74BJVQ.
TreeFam	TF319837.
Gene expression databases
ArrayExpress	O35426.
Bgee	O35426.
CleanEx	MM_XBP1.
Genevestigator	O35426.
Family and domain databases
InterPro	IPR004827. bZIP. [Graphical view]
Pfam	PF07716. bZIP_2. 1 hit. [Graphical view]
SMART	SM00338. BRLZ. 1 hit. [Graphical view]
PROSITE	PS50217. BZIP. 1 hit. PS00036. BZIP_BASIC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	XBP1. mouse.
NextBio	302881.
PRO	O35426.
SOURCE	Search...

Entry information

Entry name

XBP1_MOUSE

Accession

Primary (citable) accession number: O35426
Secondary accession number(s): Q8VHM0, Q922G5, Q9ESS3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	June 1, 2001
Last modified:	April 16, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program