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UniProtKB - O35426 (XBP1_MOUSE)

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Protein

X-box-binding protein 1

Gene

Xbp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a transcription factor during endoplasmic reticulum stress by regulating the unfolded protein response (UPR). Required for cardiac myogenesis and hepatogenesis during embryonic development and the development of secretory tissues such as exocrine pancreas and salivary gland (PubMed:10425189, PubMed:10652269, PubMed:16362047, PubMed:17612490). Involved in differentiation of B lymphocytes to plasma cells and production of immunoglobulins. Modulates the cellular response to ER stress in a PIK3R-dependent manner. Binds to the cis-acting X box present in the promoter regions of major histocompatibility complex class II genes (By similarity). Involved in VEGF-induced endothelial cell (EC) proliferation and retinal blood vessel formation during embryonic development but also for angiogenesis in adult tissues under ischemic conditions (PubMed:23529610). Functions also as a major regulator of the UPR in obesity-induced insulin resistance and type 2 diabetes for the management of obesity and diabetes prevention (PubMed:15486293).By similarity6 Publications
Isoform 1: plays a role in the unconventional cytoplasmic splicing processing of its own mRNA triggered by the endoplasmic reticulum (ER) transmembrane endoribonuclease ENR1: upon ER stress, the emerging XBP1 polypeptide chain, as part of a mRNA-ribosome-nascent chain (R-RNC) complex, cotranslationally recruits its own unprocessed mRNA through transient docking to the ER membrane and translational pausing, therefore facilitating efficient IRE1-mediated XBP1 mRNA isoform 2 production. In endothelial cells (EC), associated with KDR, promotes IRE1-mediated XBP1 mRNA isoform 2 production in a vascular endothelial growth factor (VEGF)-dependent manner, leading to EC proliferation and angiogenesis (By similarity). Functions as a negative feed-back regulator of the potent transcription factor XBP1 isoform 2 protein levels through proteasome-mediated degradation, thus preventing the constitutive activation of the ER stress response signaling pathway (PubMed:16332684). Inhibits the transactivation activity of XBP1 isoform 2 in myeloma cells (PubMed:12902539). Acts as a weak transcriptional factor. Together with HDAC3, contributes to the activation of NFE2L2-mediated HMOX1 transcription factor gene expression in a PI3K/mTORC2/Akt-dependent signaling pathway leading to EC survival under disturbed flow/oxidative stress. Binds to the ER stress response element (ERSE) upon ER stress. Binds to the consensus 5'-GATGACGTG[TG]N3[AT]T-3' sequence related to cAMP responsive element (CRE)-like sequences. Binds the Tax-responsive element (TRE) present in the long terminal repeat (LTR) of T-cell leukemia virus type 1 (HTLV-I) and to the TPA response elements (TRE). Associates preferentially to the HDAC3 gene promoter region in a static flow-dependent manner. Binds to the CDH5/VE-cadherin gene promoter region (By similarity).By similarity2 Publications
Isoform 2: functions as a stress-inducible potent transcriptional activator during endoplasmic reticulum (ER) stress by inducing unfolded protein response (UPR) target genes via binding to the UPR element (UPRE). Up-regulates target genes encoding ER chaperones and ER-associated degradation (ERAD) components to enhance the capacity of productive folding and degradation mechanism, respectively, in order to maintain the homeostasis of the ER under ER stress (PubMed:11850408, PubMed:14559994). Plays a role in the production of immunoglobulins and interleukin-6 in the presence of stimuli required for plasma cell differentiation, and promotes as well membrane phospholipid biosynthesis necessary for ER expansion (PubMed:12612580, PubMed:17213183). Contributes to the VEGF-induced endothelial cell (EC) growth and proliferation in a Akt/GSK-dependent and/or -independent signaling pathway, respectively, leading to beta-catenin nuclear translocation and E2F2 gene expression. Promotes umbilical vein EC apoptosis and atherosclerotisis development in a caspase-dependent signaling pathway, and contributes to VEGF-induced EC proliferation and angiogenesis in adult tissues under ischemic conditions. Involved in the regulation of endostatin-induced autophagy in EC through BECN1 transcriptional activation. Plays a role as an oncogene by promoting tumor progression: stimulates zinc finger protein SNAI1 transcription to induce epithelial-to-mesenchymal (EMT) transition, cell migration and invasion of breast cancer cells (By similarity). Involved in adipocyte differentiation by regulating lipogenic gene expression during lactation (PubMed:23623498, PubMed:25223794). Plays a role in the survival of both dopaminergic neurons of the substantia nigra pars compacta (SNpc), by maintaining protein homeostasis and of myeloma cells (PubMed:12902539, PubMed:24753614). Increases insulin sensitivity in the liver as a response to a high carbohydrate diet, resulting in improved glucose tolerance (PubMed:20348926). Improves also glucose homeostasis in an ER stress- and/or insulin-independent manner through both binding and proteasome-induced degradation of the transcription factor FOXO1, hence resulting in suppression of gluconeogenic genes expression and in a reduction of blood glucose levels (PubMed:21317886). Controls the induction of de novo fatty acid synthesis in hepatocytes by regulating the expression of a subset of lipogenic genes in an ER stress- and UPR-independent manner (PubMed:18556558). Binds to the 5'-CCACG-3' motif in the PPARG promoter (PubMed:25223794). Associates preferentially to the HDAC3 gene promoter region in a disturbed flow-dependent manner. Binds to the BECN1 gene promoter region. Binds to the CDH5/VE-cadherin gene promoter region. Binds to the ER stress response element (ERSE) upon ER stress (By similarity).By similarity11 Publications

GO - Molecular functioni

  • chromatin DNA binding Source: UniProtKB
  • core promoter binding Source: UniProtKB
  • enhancer sequence-specific DNA binding Source: UniProtKB
  • histone deacetylase binding Source: UniProtKB
  • phosphatidylinositol 3-kinase regulatory subunit binding Source: UniProtKB
  • protease binding Source: MGI
  • protein heterodimerization activity Source: UniProtKB
  • protein kinase binding Source: MGI
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: UniProtKB
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: MGI
  • RNA polymerase II transcription factor binding Source: MGI
  • sequence-specific DNA binding Source: ParkinsonsUK-UCL
  • transcription factor activity, sequence-specific DNA binding Source: ParkinsonsUK-UCL
  • transcription regulatory region DNA binding Source: UniProtKB
  • ubiquitin-like protein ligase binding Source: ParkinsonsUK-UCL
  • ubiquitin protein ligase binding Source: MGI
Complete GO annotation...

GO - Biological processi

  • adipose tissue development Source: UniProtKB
  • angiogenesis Source: UniProtKB
  • autophagy Source: UniProtKB-KW
  • cell growth Source: UniProtKB
  • cellular response to amino acid stimulus Source: UniProtKB
  • cellular response to fluid shear stress Source: UniProtKB
  • cellular response to fructose stimulus Source: UniProtKB
  • cellular response to glucose starvation Source: UniProtKB
  • cellular response to glucose stimulus Source: UniProtKB
  • cellular response to insulin stimulus Source: UniProtKB
  • cellular response to interleukin-4 Source: UniProtKB
  • cellular response to laminar fluid shear stress Source: UniProtKB
  • cellular response to lipopolysaccharide Source: UniProtKB
  • cellular response to nutrient Source: UniProtKB
  • cellular response to oxidative stress Source: UniProtKB
  • cellular response to peptide hormone stimulus Source: UniProtKB
  • cellular response to vascular endothelial growth factor stimulus Source: UniProtKB
  • cellular triglyceride homeostasis Source: UniProtKB
  • cholesterol homeostasis Source: UniProtKB
  • endoplasmic reticulum unfolded protein response Source: UniProtKB
  • endothelial cell proliferation Source: UniProtKB
  • epithelial cell maturation Source: MGI
  • epithelial cell maturation involved in salivary gland development Source: MGI
  • exocrine pancreas development Source: MGI
  • fatty acid homeostasis Source: UniProtKB
  • glucose homeostasis Source: MGI
  • intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: ParkinsonsUK-UCL
  • lipid metabolic process Source: UniProtKB-KW
  • liver development Source: UniProtKB
  • muscle organ development Source: UniProtKB-KW
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway Source: MGI
  • negative regulation of endoplasmic reticulum unfolded protein response Source: UniProtKB
  • negative regulation of ERK1 and ERK2 cascade Source: MGI
  • negative regulation of myotube differentiation Source: UniProtKB
  • negative regulation of pathway-restricted SMAD protein phosphorylation Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • negative regulation of transforming growth factor beta receptor signaling pathway Source: MGI
  • neuron development Source: UniProtKB
  • phosphatidylinositol 3-kinase signaling Source: UniProtKB
  • positive regulation of angiogenesis Source: MGI
  • positive regulation of autophagy Source: UniProtKB
  • positive regulation of B cell differentiation Source: UniProtKB
  • positive regulation of cell migration Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of endoplasmic reticulum unfolded protein response Source: MGI
  • positive regulation of endothelial cell apoptotic process Source: UniProtKB
  • positive regulation of fat cell differentiation Source: UniProtKB
  • positive regulation of hepatocyte proliferation Source: UniProtKB
  • positive regulation of histone methylation Source: UniProtKB
  • positive regulation of immunoglobulin production Source: UniProtKB
  • positive regulation of immunoglobulin secretion Source: UniProtKB
  • positive regulation of interleukin-6 secretion Source: UniProtKB
  • positive regulation of lactation Source: UniProtKB
  • positive regulation of MHC class II biosynthetic process Source: UniProtKB
  • positive regulation of plasma cell differentiation Source: UniProtKB
  • positive regulation of proteasomal protein catabolic process Source: MGI
  • positive regulation of protein acetylation Source: UniProtKB
  • positive regulation of protein phosphorylation Source: UniProtKB
  • positive regulation of T cell differentiation Source: UniProtKB
  • positive regulation of TOR signaling Source: UniProtKB
  • positive regulation of transcription factor import into nucleus Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response Source: UniProtKB
  • positive regulation of vascular wound healing Source: MGI
  • protein destabilization Source: UniProtKB
  • protein transport Source: UniProtKB-KW
  • regulation of autophagy Source: UniProtKB
  • regulation of protein stability Source: UniProtKB
  • regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • response to endoplasmic reticulum stress Source: UniProtKB
  • response to insulin-like growth factor stimulus Source: UniProtKB
  • sterol homeostasis Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
  • transcription from RNA polymerase II promoter Source: UniProtKB
  • ubiquitin-dependent protein catabolic process Source: UniProtKB
  • vascular endothelial growth factor receptor signaling pathway Source: UniProtKB
Complete GO annotation...

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processAngiogenesis, Apoptosis, Autophagy, Differentiation, Lipid biosynthesis, Lipid metabolism, Myogenesis, Protein transport, Stress response, Transcription, Transcription regulation, Transport, Unfolded protein response

Names & Taxonomyi

Protein namesi
Recommended name:
X-box-binding protein 1By similarityImported
Short name:
XBP-1By similarity
Alternative name(s):
Tax-responsive element-binding protein 51 Publication
Short name:
TREB-5Curated
Cleaved into the following 2 chains:
X-box-binding protein 1, cytoplasmic formBy similarity
X-box-binding protein 1, luminal formBy similarity
Gene namesi
Name:Xbp1By similarityImported
Synonyms:Treb51 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:98970. Xbp1.

Subcellular locationi

  • Endoplasmic reticulum By similarity

    • Note: Colocalizes with ERN1 and KDR in the endoplasmic reticulum in endothelial cells in a vascular endothelial growth factor (VEGF)-dependent manner (By similarity).By similarity
Isoform 1 :
  • Nucleus By similarity1 Publication
  • Cytoplasm By similarity1 Publication
  • Endoplasmic reticulum membrane By similarity; Single-pass type II membrane protein By similarity
  • Endoplasmic reticulum membrane By similarity; Peripheral membrane protein By similarity
  • Membrane By similarity; Peripheral membrane protein By similarity

    • Note: Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner. Localizes predominantly at the endoplasmic reticulum membrane as a membrane-spanning protein; whereas may be only marginally localized on the cytosolic side of the ER membrane as a peripheral membrane (By similarity). Shows no preferential localization to either the nucleus or the cytoplasm (PubMed:16332684).By similarity1 Publication
Isoform 2 :
X-box-binding protein 1, cytoplasmic form :
  • Cytoplasm By similarity
  • Nucleus By similarity

    • Note: Localizes in the cytoplasm and nucleus after HM13/SPP-mediated intramembranaire proteolytic cleavage of isoform 1 (By similarity).By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 180CytoplasmicBy similarityAdd BLAST180
Transmembranei181 – 198Helical; Signal-anchor for type II membrane proteinSequence analysisBy similarityAdd BLAST18
Topological domaini199 – 267LumenalBy similarityAdd BLAST69

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: UniProtKB
  • endoplasmic reticulum Source: UniProtKB
  • integral component of endoplasmic reticulum membrane Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice embryos die at 12.5-13.5 dpc and display less blood vessels (PubMed:23529610). Embryos display hypoplastic livers, cellular necrosis in the myocardium, hypoplasia of the heart and die in utero from severe anemia (PubMed:10425189, PubMed:10652269). Mice display severe abnormalities in the development and function of secretory cells, such as plasma B cells and pancreatic acinar cells (PubMed:16362047). Haploinsufficient mice fed a high-fat diet gain more weight, display enhanced ER stress in adipose tissue, reduced insulin receptor signaling and develop peripheral insulin resistance and type 2 diabetes (PubMed:15486293). Endothelial-specific knockout mice show delayed retinal vascular development and impaired postischemic angiogenesis (PubMed:23529610, PubMed:23184933). Dopaminergic neuron-specific knockout mice display ER dysfonction and accumulation of abnormal protein aggregates (PubMed:24753614). Liver-specific knockout mice leads to reduced lipogenic gene expression and diminished hepatic lipid synthesis (PubMed:18556558). Adipocyte-specific knockout female mice fed with a regular or high-fat diet, show no alteration in body weight, adipose tissue mass, blood glucose, serum insulin and lipid levels; however during lactation adipose tissue mass increases and milk production decreases but mammary gland structure and milk composition remains normal (PubMed:23623498). Intestinal epithelial cell-specific knockout mice born and developed normally but displayed small intestinal mucosal inflammation in association with increased ER stress, a diminution of Paneth and goblet cells with reduced secretory granules (PubMed:18775308).10 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi177 – 178PP → LH: Reduces degradation (isoform 1); when associated with 238-L-H-239. 1 Publication2
Mutagenesisi231K → R: Enhances stability and accumulates in the cytoplasm; when associated with R-252 (isoform 1). 2 Publications1
Mutagenesisi238 – 239PP → LH: Reduces degradation (isoform 1); when associated with 177-L-H-178. 1 Publication2
Mutagenesisi252K → R: Enhances stability and accumulates in the cytoplasm; when associated with R-231 (isoform 1). 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000765441 – 267X-box-binding protein 1Add BLAST267
ChainiPRO_00004318931 – 188X-box-binding protein 1, cytoplasmic formBy similarityAdd BLAST188
ChainiPRO_0000431894191 – 267X-box-binding protein 1, luminal formBy similarityAdd BLAST77

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei61PhosphoserineBy similarity1

Post-translational modificationi

Isoform 2 is acetylated by EP300; acetylation positively regulates the transcriptional activity of XBP1 isoform 2 (PubMed:20955178). Isoform 2 is deacetylated by SIRT1; deacetylation negatively regulates the transcriptional activity of XBP1 isoform 2 (PubMed:20955178).1 Publication1 Publication
Isoform 1 is ubiquitinated, leading to proteasomal degradation in response to ER stress (PubMed:11780124, PubMed:12902539, PubMed:16332684).By similarity3 Publications
X-box-binding protein 1, cytoplasmic form and luminal form are produced by intramembrane proteolytic cleavage of ER membrane-anchored isoform 1 triggered by HM13/SPP in a DERL1-RNF139-dependent and VCP/p97-independent manner. X-box-binding protein 1, luminal form is ubiquitinated leading to proteasomal degradation (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei185 – 186Cleavage; by HM13/SPPBy similarity2

Keywords - PTMi

Acetylation, Cleavage on pair of basic residues, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO35426.
PRIDEiO35426.

PTM databases

iPTMnetiO35426.
PhosphoSitePlusiO35426.

Expressioni

Tissue specificityi

Isoform 1 and isoform 2 are expressed at higher level in branch curves of vessel walls and in atherosclerotic plaques relative to healthy segments of the same aortas (at protein level) (PubMed:19416856). Expressed in skeletal muscles, plasma cells and pancreatic beta cells (PubMed:17612490). Isoform 1 and isoform 2 are expressed in gonadal adipose tissue. Isoform 1 is expressed in inguinal adipose tissue (PubMed:23623498).3 Publications

Developmental stagei

Expressed mainly in exocrine glands and bone precursors in the embryonic mouse (PubMed:7693055).1 Publication

Inductioni

Isoform 2 is up-regulated during adipocyte differentiation (PubMed:25223794). Isoform 2 is up-regulated upon refeeding after a fasting period in liver and in ob/ob mice (obese) (at protein level) (PubMed:20348926). Induced by chemical activators of the unfolded protein response (UPR) such as tunicamycin, DTT and thapsigargin (PubMed:17612490). Up-regulated after partial hepatectomy during the acute phase response (PubMed:10652269). Isoform 1 and isoform 2 are up-regulated by interleukin-4 in B cells in a STAT6-dependent manner (PubMed:12612580). Isoform 1 and isoform 2 are up-regulated during lactation and by the lactogenic hormone prolactin (PubMed:23623498). Isoform 2 is up-regulated by prolonged feeding of high-carbohydrate diets in hepatocytes in absence of ER-stress (PubMed:18556558). Isoform 2 is up-regulated by insulin-like growth factor and glucose starvation (PubMed:17612490). Isoform 2 is up-regulated during plasma-cell differentiation in response to endoplasmic reticulum (ER) stress, such as lipopolysaccharide (LPS) (PubMed:11780124, PubMed:11850408, PubMed:12612580).9 Publications

Gene expression databases

BgeeiENSMUSG00000020484.
CleanExiMM_XBP1.
ExpressionAtlasiO35426. baseline and differential.
GenevisibleiO35426. MM.

Interactioni

Subunit structurei

Isoform 1 interacts with HM13. Isoform 1 interacts with RNF139; the interaction induces ubiquitination and degradation of isoform 1. Isoform 1 interacts (via luminal domain) with DERL1; the interaction obviates the need for ectodomain shedding prior HM13/SPP-mediated XBP1 isoform 1 cleavage. Isoform 1 interacts with isoform 2; the interaction sequesters isoform 2 from the nucleus and enhances isoform 2 degradation in the cytoplasm. Isoform 1 interacts with HDAC3 and AKT1; the interactions occur in endothelial cell (EC) under disturbed flow. Isoform 1 interacts with the oncoprotein FOS. Isoform 2 interacts with ATF6; the interaction occurs in a ER stress-dependent manner and is required for DNA binding to the unfolded protein response element (UPRE). Isoform 2 interacts with PIK3R1; the interaction is direct and induces translocation of XBP1 isoform 2 into the nucleus and the unfolded protein response (UPR) XBP1-dependent target genes activation in a ER stress- and/or insulin-dependent but PI3K-independent manner (By similarity). Isoform 2 interacts with SIRT1 (PubMed:20955178). Isoform 2 interacts with PIK3R1 and PIK3R2; the interactions are direct and induce translocation of XBP1 isoform 2 into the nucleus and the unfolded protein response (UPR) XBP1-dependent target genes activation in a ER stress- and/or insulin-dependent but PI3K-independent manner (PubMed:20348926). Isoform 2 interacts with FOXO1; the interaction is direct and leads to FOXO1 ubiquitination and degradation via the proteasome pathway in hepatocytes (PubMed:21317886).By similarity3 Publications

GO - Molecular functioni

  • histone deacetylase binding Source: UniProtKB
  • phosphatidylinositol 3-kinase regulatory subunit binding Source: UniProtKB
  • protease binding Source: MGI
  • protein heterodimerization activity Source: UniProtKB
  • protein kinase binding Source: MGI
  • RNA polymerase II transcription factor binding Source: MGI
  • ubiquitin-like protein ligase binding Source: ParkinsonsUK-UCL
  • ubiquitin protein ligase binding Source: MGI
Complete GO annotation...

Protein-protein interaction databases

BioGridi204589. 2 interactors.
STRINGi10090.ENSMUSP00000054852.

Structurei

3D structure databases

ProteinModelPortaliO35426.
SMRiO35426.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 126bZIPPROSITE-ProRule annotationAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni65 – 87Basic motifPROSITE-ProRule annotationAdd BLAST23
Regioni69 – 85Nuclear localization signal (NLS); in isoforms 1 and isoform 2By similarityAdd BLAST17
Regioni91 – 126Leucine-zipperPROSITE-ProRule annotationAdd BLAST36
Regioni230 – 256Necessary for the translational pausing of its own mRNABy similarityAdd BLAST27

Domaini

Isoform 1 transmembrane signal-anchor domain is necessary for its own mRNA to be recruited to the endoplasmic reticulum (ER) which will undergo unconventional ERN1-dependent splicing in response to ER stress. Isoform 1 N-terminus and C-terminus regions are necessary for DNA-binding and weak transcriptional activity, respectively. Isoform 2 N-terminus and C-terminus regions are necessary for DNA-binding and strong transcriptional activity upon ER stress, respectively. Isoform 2 C-terminus region contains a nuclear exclusion signal (NES) at positions 182 through 204. Isoform 2 C-terminus region contains a degradation domain at positions 204 through 256 (By similarity). Isoform 1 and isoform 2 N-terminus domains are necessary for nuclear localization targeting. Isoform 1 C-terminus domain confers localization to the cytoplasm and is sufficient to impose rapid degradation (PubMed:16332684).By similarity1 Publication

Sequence similaritiesi

Belongs to the bZIP family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4005. Eukaryota.
ENOG410XSJI. LUCA.
GeneTreeiENSGT00390000017751.
HOGENOMiHOG000007671.
HOVERGENiHBG061457.
InParanoidiO35426.
KOiK09027.
OMAiNQELRCR.
OrthoDBiEOG091G0JHJ.
TreeFamiTF319837.

Family and domain databases

InterProiView protein in InterPro
IPR004827. bZIP.
PfamiView protein in Pfam
PF07716. bZIP_2. 1 hit.
SMARTiView protein in SMART
SM00338. BRLZ. 1 hit.
PROSITEiView protein in PROSITE
PS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O35426-1) [UniParc]FASTAAdd to basket
Also known as: Unprocessed XBP-1, XBP-1U1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVVVAAAPSA ATAAPKVLLL SGQPASGGRA LPLMVPGPRA AGSEASGTPQ 
        60         70         80         90        100
ARKRQRLTHL SPEEKALRRK LKNRVAAQTA RDRKKARMSE LEQQVVDLEE 
       110        120        130        140        150
ENHKLQLENQ LLREKTHGLV VENQELRTRL GMDTLDPDEV PEVEAKGSGV 
       160        170        180        190        200
RLVAGSAESA ALRLCAPLQQ VQAQLSPPQN IFPWTLTLLP LQILSLISFW 
       210        220        230        240        250
AFWTSWTLSC FSNVLPQSLL VWRNSQRSTQ KDLVPYQPPF LCQWGPHQPS 
       260 
WKPLMNSFVL TMYTPSL
Length:267
Mass (Da):29,619
Last modified:June 1, 2001 - v2
Checksum:i15009E684F6D426F
GO
Isoform 2 (identifier: O35426-2) [UniParc]FASTAAdd to basket
Also known as: Processed XBP-1, XBP-1S1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     162-267: LRLCAPLQQV...FVLTMYTPSL → GAGPVVTSPE...NELFPQLISV

Note: Potent transcriptional activator. Induced by unconventional ERN1-dependent splicing in response to endoplasmic reticulum stress. ENR1 cleaves a 26-bp fragment causing a frameshift of the mRNA transcript (PubMed:11780124, PubMed:11850408).2 Publications
Show »
Length:371
Mass (Da):39,888
Checksum:i6F67EC346DBF3AC8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27Missing in BAB13793 (PubMed:10907849).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012937162 – 267LRLCA…YTPSL → GAGPVVTSPEHLPMDSDTVA SSDSESDILLGILDKLDPVM FFKCPSPESASLEELPEVYP EGPSSLPASLSLSVGTSSAK LEAINELIRFDHVYTKPLVL EIPSETESQTNVVVKIEEAP LSSSEEDHPEFIVSVKKEPL EDDFIPELGISNLLSSSHCL RPPSCLLDAHSDCGYEGSPS PFSDMSSPLGTDHSWEDTFA NELFPQLISV in isoform 2. 1 PublicationAdd BLAST106

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB036745 Genomic DNA. Translation: BAB13793.1.
AF443192 mRNA. Translation: AAL60202.1.
AF027963 mRNA. Translation: AAB81862.2.
AL662876 Genomic DNA. Translation: CAI24935.1.
BC008153 mRNA. Translation: AAH08153.1.
BC029197 mRNA. Translation: AAH29197.1.
CCDSiCCDS24400.1. [O35426-1]
PIRiJC7300.
RefSeqiNP_001258659.1. NM_001271730.1. [O35426-2]
NP_038870.2. NM_013842.3. [O35426-1]
UniGeneiMm.469937.

Genome annotation databases

EnsembliENSMUST00000063084; ENSMUSP00000054852; ENSMUSG00000020484. [O35426-1]
GeneIDi22433.
KEGGimmu:22433.
UCSCiuc007hwm.2. mouse. [O35426-2]
uc007hwn.2. mouse. [O35426-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiXBP1_MOUSE
AccessioniPrimary (citable) accession number: O35426
Secondary accession number(s): Q8VHM0, Q922G5, Q9ESS3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: June 1, 2001
Last modified: May 10, 2017
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families