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O35426 (XBP1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
X-box-binding protein 1

Short name=XBP-1
Alternative name(s):
Tax-responsive element-binding protein 5 homolog
Gene names
Name:Xbp1
Synonyms:Treb5
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length267 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N3[AT]T-3', and also to some TPA response elements (TRE) By similarity. Isoform 2 is involved in controlling the production of interleukin-6. Ref.6 Ref.7

Subcellular location

Nucleus.

Induction

Up-regulated in response to endoplasmic reticulum stress. Induced by interleukin-4 in plasma cell differentiation. Ref.2 Ref.7

Sequence similarities

Belongs to the bZIP family.

Contains 1 bZIP (basic-leucine zipper) domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   LigandDNA-binding
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to antibiotic

Inferred from electronic annotation. Source: Ensembl

endoplasmic reticulum unfolded protein response

Inferred from electronic annotation. Source: Ensembl

epithelial cell maturation

Inferred from mutant phenotype PubMed 16362047. Source: MGI

epithelial cell maturation involved in salivary gland development

Inferred from mutant phenotype PubMed 16362047. Source: MGI

exocrine pancreas development

Inferred from mutant phenotype PubMed 16362047. Source: MGI

glucose homeostasis

Inferred from direct assay PubMed 21317886. Source: MGI

positive regulation of endoplasmic reticulum unfolded protein response

Inferred from electronic annotation. Source: Ensembl

positive regulation of proteasomal protein catabolic process

Inferred from direct assay PubMed 21317886. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 23152784. Source: MGI

response to drug

Inferred from electronic annotation. Source: Ensembl

response to electrical stimulus

Inferred from electronic annotation. Source: Ensembl

serotonin secretion, neurotransmission

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionRNA polymerase II transcription factor binding

Inferred from physical interaction PubMed 21317886. Source: MGI

sequence-specific DNA binding

Inferred from electronic annotation. Source: Ensembl

sequence-specific DNA binding RNA polymerase II transcription factor activity

Inferred from direct assay PubMed 23152784. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O35426-1)

Also known as: XBP-1U;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O35426-2)

Also known as: XBP-1S;

The sequence of this isoform differs from the canonical sequence as follows:
     162-267: LRLCAPLQQV...FVLTMYTPSL → GAGPVVTSPE...NELFPQLISV
Note: Potent transcriptional activator. Induced by ERN1 in response to endoplasmic reticulum stress. ERN1 cleaves a 26-bp fragment causing a frameshift of the mRNA transcript.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 267267X-box-binding protein 1
PRO_0000076544

Regions

Domain63 – 12664bZIP
Region65 – 8723Basic motif By similarity
Region91 – 12636Leucine-zipper By similarity

Natural variations

Alternative sequence162 – 267106LRLCA…YTPSL → GAGPVVTSPEHLPMDSDTVA SSDSESDILLGILDKLDPVM FFKCPSPESASLEELPEVYP EGPSSLPASLSLSVGTSSAK LEAINELIRFDHVYTKPLVL EIPSETESQTNVVVKIEEAP LSSSEEDHPEFIVSVKKEPL EDDFIPELGISNLLSSSHCL RPPSCLLDAHSDCGYEGSPS PFSDMSSPLGTDHSWEDTFA NELFPQLISV in isoform 2.
VSP_012937

Experimental info

Sequence conflict271Missing in BAB13793. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (XBP-1U) [UniParc].

Last modified June 1, 2001. Version 2.
Checksum: 15009E684F6D426F

FASTA26729,619
        10         20         30         40         50         60 
MVVVAAAPSA ATAAPKVLLL SGQPASGGRA LPLMVPGPRA AGSEASGTPQ ARKRQRLTHL 

        70         80         90        100        110        120 
SPEEKALRRK LKNRVAAQTA RDRKKARMSE LEQQVVDLEE ENHKLQLENQ LLREKTHGLV 

       130        140        150        160        170        180 
VENQELRTRL GMDTLDPDEV PEVEAKGSGV RLVAGSAESA ALRLCAPLQQ VQAQLSPPQN 

       190        200        210        220        230        240 
IFPWTLTLLP LQILSLISFW AFWTSWTLSC FSNVLPQSLL VWRNSQRSTQ KDLVPYQPPF 

       250        260 
LCQWGPHQPS WKPLMNSFVL TMYTPSL 

« Hide

Isoform 2 (XBP-1S) [UniParc].

Checksum: 6F67EC346DBF3AC8
Show »

FASTA37139,888

References

« Hide 'large scale' references
[1]"Isolation and characterization of the gene encoding mouse tax-responsive element-binding protein (TREB) 5."
Masaki T., Noguchi H., Kobayashi M., Yoshida M., Takamatsu K.
DNA Res. 7:187-193(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA."
Calfon M., Zeng H., Urano F., Till J.H., Hubbard S.R., Harding H.P., Clark S.G., Ron D.
Nature 415:92-96(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), INDUCTION.
Strain: 129/SvEv.
[3]"Sequence analysis of murine XBP-1."
Lee C.M., Reddy E.P.
Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Mammary tumor.
[6]"XBP-1 regulates a subset of endoplasmic reticulum resident chaperone genes in the unfolded protein response."
Lee A.H., Iwakoshi N.N., Glimcher L.H.
Mol. Cell. Biol. 23:7448-7459(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Plasma cell differentiation and the unfolded protein response intersect at the transcription factor XBP-1."
Iwakoshi N.N., Lee A.-H., Vallabhajosyula P., Otipoby K.L., Rajewsky K., Glimcher L.H.
Nat. Immunol. 4:321-329(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB036745 Genomic DNA. Translation: BAB13793.1.
AF443192 mRNA. Translation: AAL60202.1.
AF027963 mRNA. Translation: AAB81862.2.
AL662876 Genomic DNA. Translation: CAI24935.1.
BC008153 mRNA. Translation: AAH08153.1.
BC029197 mRNA. Translation: AAH29197.1.
PIRJC7300.
RefSeqNP_001258659.1. NM_001271730.1.
NP_038870.2. NM_013842.3.
UniGeneMm.469937.

3D structure databases

ProteinModelPortalO35426.
SMRO35426. Positions 64-126.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204589. 1 interaction.

PTM databases

PhosphoSiteO35426.

Proteomic databases

PRIDEO35426.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000063084; ENSMUSP00000054852; ENSMUSG00000020484. [O35426-1]
GeneID22433.
KEGGmmu:22433.
UCSCuc007hwm.1. mouse. [O35426-1]

Organism-specific databases

CTD7494.
MGIMGI:98970. Xbp1.

Phylogenomic databases

eggNOGNOG285368.
GeneTreeENSGT00390000017751.
HOGENOMHOG000007671.
HOVERGENHBG061457.
KOK09027.
OMAWKPLMNS.
OrthoDBEOG74BJVQ.
TreeFamTF319837.

Gene expression databases

ArrayExpressO35426.
BgeeO35426.
CleanExMM_XBP1.
GenevestigatorO35426.

Family and domain databases

InterProIPR004827. bZIP.
[Graphical view]
PfamPF07716. bZIP_2. 1 hit.
[Graphical view]
SMARTSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSXBP1. mouse.
NextBio302881.
PROO35426.
SOURCESearch...

Entry information

Entry nameXBP1_MOUSE
AccessionPrimary (citable) accession number: O35426
Secondary accession number(s): Q8VHM0, Q922G5, Q9ESS3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot