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Protein

Bcl-2-related ovarian killer protein

Gene

Bok

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Induces apoptosis in a manner dependent on BAX and BAK.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Bcl-2-related ovarian killer protein
Alternative name(s):
Apoptosis activator Mtd
Protein matador
Gene namesi
Name:Bok
Synonyms:Mtd
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1858494. Bok.

Subcellular locationi

  • Endoplasmic reticulum membrane By similarity; Single-pass membrane protein By similarity
  • Golgi apparatus membrane By similarity; Single-pass membrane protein By similarity

  • Note: The transmembrane domain is necessary and sufficient for Golgi and ER targeting.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei190 – 21021HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi71 – 711L → Q: No change in apoptosis induction; when associated with Q-74 and Q-78. 1 Publication
Mutagenesisi74 – 741L → Q: No change in apoptosis induction; when associated with Q-71 and Q-78. 1 Publication
Mutagenesisi78 – 781L → Q: No change in apoptosis induction; when associated with Q-71 and Q-74. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 213213Bcl-2-related ovarian killer proteinPRO_0000143087Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei7 – 71PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO35425.
PRIDEiO35425.

PTM databases

iPTMnetiO35425.
PhosphoSiteiO35425.

Expressioni

Tissue specificityi

Expressed in brain, liver, lymphoid tissues and in embryonic intestinal epithelium.1 Publication

Developmental stagei

At E15.0, expressed in brain, liver, thymus, lung, intestinal epithelium and follicles of the whiskers.1 Publication

Gene expression databases

BgeeiO35425.
CleanExiMM_BOK.
ExpressionAtlasiO35425. baseline and differential.
GenevisibleiO35425. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000027499.

Structurei

3D structure databases

ProteinModelPortaliO35425.
SMRiO35425. Positions 45-176.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi32 – 4413BH4Add
BLAST
Motifi67 – 8317BH3Add
BLAST
Motifi113 – 13220BH1Add
BLAST
Motifi165 – 17915BH2Add
BLAST

Sequence similaritiesi

Belongs to the Bcl-2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4728. Eukaryota.
ENOG41123S0. LUCA.
HOGENOMiHOG000290675.
HOVERGENiHBG050709.
InParanoidiO35425.
KOiK02561.
OMAiCRDYIHS.
OrthoDBiEOG7FZ00G.
PhylomeDBiO35425.
TreeFamiTF315834.

Family and domain databases

InterProiIPR002475. Bcl2-like.
IPR026298. Blc2_fam.
IPR026309. BOK.
[Graphical view]
PANTHERiPTHR11256. PTHR11256. 1 hit.
PTHR11256:SF48. PTHR11256:SF48. 1 hit.
PfamiPF00452. Bcl-2. 1 hit.
[Graphical view]
PRINTSiPR01862. BCL2FAMILY.
PROSITEiPS50062. BCL2_FAMILY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35425-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVLRRSSVF AAEIMDAFDR SPTDKELVAQ AKALGREYVH ARLLRAGLSW
60 70 80 90 100
SAPERASPAP GGRLAEVCTV LLRLGDELEQ IRPSVYRNVA RQLHIPLQSE
110 120 130 140 150
PVVTDAFLAV AGHIFSAGIT WGKVVSLYSV AAGLAVDCVR QAQPAMVHAL
160 170 180 190 200
VDCLGEFVRK TLATWLRRRG GWTDVLKCVV STDPGFRSHW LVATLCSFGR
210
FLKAAFFLLL PER
Length:213
Mass (Da):23,456
Last modified:January 1, 1998 - v1
Checksum:iF8755C45CB05D626
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF027707 mRNA. Translation: AAC53582.1.
BC030069 mRNA. Translation: AAH30069.1.
CCDSiCCDS15193.1.
RefSeqiNP_058058.1. NM_016778.3.
UniGeneiMm.3295.

Genome annotation databases

EnsembliENSMUST00000027499; ENSMUSP00000027499; ENSMUSG00000026278.
ENSMUST00000201863; ENSMUSP00000144347; ENSMUSG00000026278.
GeneIDi51800.
KEGGimmu:51800.
UCSCiuc007cee.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF027707 mRNA. Translation: AAC53582.1.
BC030069 mRNA. Translation: AAH30069.1.
CCDSiCCDS15193.1.
RefSeqiNP_058058.1. NM_016778.3.
UniGeneiMm.3295.

3D structure databases

ProteinModelPortaliO35425.
SMRiO35425. Positions 45-176.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000027499.

PTM databases

iPTMnetiO35425.
PhosphoSiteiO35425.

Proteomic databases

PaxDbiO35425.
PRIDEiO35425.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027499; ENSMUSP00000027499; ENSMUSG00000026278.
ENSMUST00000201863; ENSMUSP00000144347; ENSMUSG00000026278.
GeneIDi51800.
KEGGimmu:51800.
UCSCiuc007cee.1. mouse.

Organism-specific databases

CTDi666.
MGIiMGI:1858494. Bok.

Phylogenomic databases

eggNOGiKOG4728. Eukaryota.
ENOG41123S0. LUCA.
HOGENOMiHOG000290675.
HOVERGENiHBG050709.
InParanoidiO35425.
KOiK02561.
OMAiCRDYIHS.
OrthoDBiEOG7FZ00G.
PhylomeDBiO35425.
TreeFamiTF315834.

Miscellaneous databases

PROiO35425.
SOURCEiSearch...

Gene expression databases

BgeeiO35425.
CleanExiMM_BOK.
ExpressionAtlasiO35425. baseline and differential.
GenevisibleiO35425. MM.

Family and domain databases

InterProiIPR002475. Bcl2-like.
IPR026298. Blc2_fam.
IPR026309. BOK.
[Graphical view]
PANTHERiPTHR11256. PTHR11256. 1 hit.
PTHR11256:SF48. PTHR11256:SF48. 1 hit.
PfamiPF00452. Bcl-2. 1 hit.
[Graphical view]
PRINTSiPR01862. BCL2FAMILY.
PROSITEiPS50062. BCL2_FAMILY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mtd, a novel Bcl-2 family member activates apoptosis in the absence of heterodimerization with Bcl-2 and Bcl-XL."
    Inohara N., Ekhterae D., Garcia I., Carrio R., Merino J., Merry A., Chen S., Nunez G.
    J. Biol. Chem. 273:8705-8710(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, MUTAGENESIS OF LEU-71; LEU-74 AND LEU-78.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Lung.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiBOK_MOUSE
AccessioniPrimary (citable) accession number: O35425
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: January 1, 1998
Last modified: June 8, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

'Matador' means killer in Spanish.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.