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Protein

Serine--pyruvate aminotransferase, mitochondrial

Gene

Agxt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dual metabolic roles of gluconeogenesis (in the mitochondria) and glyoxylate detoxification (in the peroxisomes).By similarity

Catalytic activityi

L-serine + pyruvate = 3-hydroxypyruvate + L-alanine.
L-alanine + glyoxylate = pyruvate + glycine.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei382SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-MMU-389661. Glyoxylate metabolism and glycine degradation.
SABIO-RKO35423.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine--pyruvate aminotransferase, mitochondrial (EC:2.6.1.51)
Short name:
SPT
Alternative name(s):
Alanine--glyoxylate aminotransferase (EC:2.6.1.44)
Short name:
AGT
Gene namesi
Name:Agxt
Synonyms:Agxt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1329033. Agxt.

Subcellular locationi

  • Mitochondrion matrix By similarity
  • Peroxisome By similarity

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: HGNC
  • peroxisomal matrix Source: MGI
  • peroxisome Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 23MitochondrionAdd BLAST23
ChainiPRO_000000128825 – 414Serine--pyruvate aminotransferase, mitochondrialAdd BLAST390

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei231N6-(pyridoxal phosphate)lysineBy similarity1
Modified residuei247N6-acetyllysine; alternateCombined sources1
Modified residuei247N6-succinyllysine; alternateCombined sources1
Modified residuei256N6-acetyllysineCombined sources1
Modified residuei330N6-acetyllysine; alternateCombined sources1
Modified residuei330N6-succinyllysine; alternateCombined sources1
Modified residuei334N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO35423.
PeptideAtlasiO35423.
PRIDEiO35423.

PTM databases

iPTMnetiO35423.
PhosphoSitePlusiO35423.
SwissPalmiO35423.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026272.
CleanExiMM_AGXT.
GenevisibleiO35423. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiO35423. 2 interactors.
MINTiMINT-1859331.
STRINGi10090.ENSMUSP00000027491.

Structurei

Secondary structure

1414
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi34 – 37Combined sources4
Beta strandi49 – 51Combined sources3
Helixi57 – 62Combined sources6
Helixi73 – 90Combined sources18
Beta strandi95 – 101Combined sources7
Turni104 – 106Combined sources3
Helixi107 – 115Combined sources9
Beta strandi121 – 128Combined sources8
Helixi129 – 140Combined sources12
Beta strandi144 – 149Combined sources6
Helixi158 – 168Combined sources11
Beta strandi171 – 179Combined sources9
Turni180 – 183Combined sources4
Helixi191 – 197Combined sources7
Beta strandi201 – 205Combined sources5
Turni207 – 212Combined sources6
Turni217 – 221Combined sources5
Beta strandi224 – 231Combined sources8
Beta strandi240 – 244Combined sources5
Helixi246 – 253Combined sources8
Helixi266 – 272Combined sources7
Beta strandi276 – 278Combined sources3
Helixi288 – 304Combined sources17
Helixi306 – 326Combined sources21
Beta strandi331 – 335Combined sources5
Helixi336 – 338Combined sources3
Beta strandi341 – 347Combined sources7
Helixi354 – 365Combined sources12
Helixi374 – 376Combined sources3
Turni377 – 379Combined sources3
Beta strandi380 – 384Combined sources5
Helixi387 – 389Combined sources3
Helixi392 – 408Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KGWX-ray1.65A/B23-414[»]
3KGXX-ray1.80A/B23-414[»]
ProteinModelPortaliO35423.
SMRiO35423.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO35423.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi412 – 414Microbody targeting signalBy similarity3

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2862. Eukaryota.
COG0075. LUCA.
GeneTreeiENSGT00390000006648.
HOGENOMiHOG000171815.
HOVERGENiHBG006907.
InParanoidiO35423.
KOiK00830.
OMAiAYSCSQK.
OrthoDBiEOG091G0EMK.
TreeFamiTF313234.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000192. Aminotrans_V_dom.
IPR020578. Aminotrans_V_PyrdxlP_BS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR024169. SP_NH2Trfase/AEP_transaminase.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF000524. SPT. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Mitochondrial (identifier: O35423-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFRMLAKASV TLGSRAAGWV RTMGSYQLLV PPPEALSKPL SVPTRLLLGP
60 70 80 90 100
GPSNLAPRVL AAGSLRMIGH MQKEMLQIME EIKQGIQYVF QTRNPLTLVV
110 120 130 140 150
SGSGHCAMET ALFNLLEPGD SFLTGTNGIW GMRAAEIADR IGARVHQMIK
160 170 180 190 200
KPGEHYTLQE VEEGLAQHKP VLLFLVHGES STGVVQPLDG FGELCHRYQC
210 220 230 240 250
LLLVDSVASL GGVPIYMDQQ GIDIMYSSSQ KVLNAPPGIS LISFNDKAKY
260 270 280 290 300
KVYSRKTKPV SFYTDITYLA KLWGCEGETR VIHHTTPVTS LYCLRESLAL
310 320 330 340 350
IAEQGLENCW RRHREATAHL HKHLQEMGLK FFVKDPEIRL PTITTVTVPA
360 370 380 390 400
GYNWRDIVSY VLDHFSIEIS GGLGPTEERV LRIGLLGYNA TTENVDRVAE
410
ALREALQHCP KNKL
Length:414
Mass (Da):45,912
Last modified:April 3, 2013 - v3
Checksum:iA2AB28AC48A292E3
GO
Isoform Peroxisomal (identifier: O35423-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: Missing.

Show »
Length:392
Mass (Da):43,520
Checksum:iD7452F1A576BCA17
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti304Q → R in AAB82001 (PubMed:11225057).Curated1
Sequence conflicti348 – 349VP → A in AAB82001 (PubMed:11225057).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0186441 – 22Missing in isoform Peroxisomal. 1 PublicationAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF027730 mRNA. Translation: AAB82001.2.
AC110247 Genomic DNA. No translation available.
CH466520 Genomic DNA. Translation: EDL39972.1.
BC025799 mRNA. Translation: AAH25799.1.
CCDSiCCDS15184.1. [O35423-1]
RefSeqiNP_057911.2. NM_016702.3. [O35423-1]
UniGeneiMm.7457.

Genome annotation databases

EnsembliENSMUST00000027491; ENSMUSP00000027491; ENSMUSG00000026272. [O35423-1]
GeneIDi11611.
KEGGimmu:11611.
UCSCiuc007cdi.2. mouse. [O35423-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF027730 mRNA. Translation: AAB82001.2.
AC110247 Genomic DNA. No translation available.
CH466520 Genomic DNA. Translation: EDL39972.1.
BC025799 mRNA. Translation: AAH25799.1.
CCDSiCCDS15184.1. [O35423-1]
RefSeqiNP_057911.2. NM_016702.3. [O35423-1]
UniGeneiMm.7457.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KGWX-ray1.65A/B23-414[»]
3KGXX-ray1.80A/B23-414[»]
ProteinModelPortaliO35423.
SMRiO35423.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO35423. 2 interactors.
MINTiMINT-1859331.
STRINGi10090.ENSMUSP00000027491.

PTM databases

iPTMnetiO35423.
PhosphoSitePlusiO35423.
SwissPalmiO35423.

Proteomic databases

PaxDbiO35423.
PeptideAtlasiO35423.
PRIDEiO35423.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027491; ENSMUSP00000027491; ENSMUSG00000026272. [O35423-1]
GeneIDi11611.
KEGGimmu:11611.
UCSCiuc007cdi.2. mouse. [O35423-1]

Organism-specific databases

CTDi189.
MGIiMGI:1329033. Agxt.

Phylogenomic databases

eggNOGiKOG2862. Eukaryota.
COG0075. LUCA.
GeneTreeiENSGT00390000006648.
HOGENOMiHOG000171815.
HOVERGENiHBG006907.
InParanoidiO35423.
KOiK00830.
OMAiAYSCSQK.
OrthoDBiEOG091G0EMK.
TreeFamiTF313234.

Enzyme and pathway databases

ReactomeiR-MMU-389661. Glyoxylate metabolism and glycine degradation.
SABIO-RKO35423.

Miscellaneous databases

ChiTaRSiAgxt. mouse.
EvolutionaryTraceiO35423.
PROiO35423.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026272.
CleanExiMM_AGXT.
GenevisibleiO35423. MM.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000192. Aminotrans_V_dom.
IPR020578. Aminotrans_V_PyrdxlP_BS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR024169. SP_NH2Trfase/AEP_transaminase.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF000524. SPT. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPYA_MOUSE
AccessioniPrimary (citable) accession number: O35423
Secondary accession number(s): Q8R128
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: April 3, 2013
Last modified: November 2, 2016
This is version 134 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.