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Protein

Sorbin and SH3 domain-containing protein 2

Gene

Sorbs2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein that plays a role in the assembling of signaling complexes, being a link between ABL kinases and actin cytoskeleton. Can form complex with ABL1 and CBL, thus promoting ubiquitination and degradation of ABL1 or with AKT1 and PAK1, thus mediating AKT1-mediated activation of PAK1 (By similarity). May play a role in the regulation of pancreatic cell adhesion, possibly by acting on WASF1 phosphorylation, enhancing phosphorylation by ABL1, as well as dephosphorylation by PTPN12 (By similarity). Isoform 2 increases water and sodium absorption in the intestine and gall-bladder.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • actin filament organization Source: InterPro
  • cell adhesion Source: InterPro
  • cell growth involved in cardiac muscle cell development Source: BHF-UCL
  • cell migration Source: InterPro
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Sorbin and SH3 domain-containing protein 2
Alternative name(s):
Arg-binding protein 2
Short name:
ArgBP2
Arg/Abl-interacting protein 2
Neural ArgBP2
Short name:
nArgBP2
Sorbin
Gene namesi
Name:Sorbs2
Synonyms:Argbp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620061. Sorbs2.

Subcellular locationi

GO - Cellular componenti

  • apical plasma membrane Source: UniProtKB-SubCell
  • dendrite Source: RGD
  • focal adhesion Source: UniProtKB-SubCell
  • lamellipodium Source: UniProtKB-SubCell
  • neuronal cell body Source: RGD
  • perinuclear region of cytoplasm Source: UniProtKB-SubCell
  • postsynaptic density Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11961196Sorbin and SH3 domain-containing protein 2PRO_0000344479Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei27 – 271PhosphoserineCombined sources
Modified residuei28 – 281PhosphoserineBy similarity
Modified residuei40 – 401PhosphoserineCombined sources
Modified residuei130 – 1301PhosphoserineBy similarity
Modified residuei143 – 1431PhosphoserineCombined sources
Modified residuei254 – 2541PhosphoserineCombined sources
Modified residuei334 – 3341PhosphoserineBy similarity
Modified residuei340 – 3401PhosphoserineBy similarity
Modified residuei343 – 3431PhosphoserineCombined sources
Modified residuei354 – 3541PhosphoserineCombined sources
Modified residuei372 – 3721PhosphothreonineBy similarity
Modified residuei382 – 3821PhosphoserineBy similarity
Modified residuei387 – 3871PhosphothreonineBy similarity
Modified residuei392 – 3921PhosphoserineBy similarity
Modified residuei393 – 3931PhosphoserineBy similarity
Modified residuei394 – 3941PhosphoserineBy similarity
Modified residuei396 – 3961PhosphoserineBy similarity
Modified residuei397 – 3971PhosphoserineCombined sources
Modified residuei399 – 3991PhosphoserineBy similarity
Modified residuei478 – 4781PhosphoserineBy similarity
Modified residuei589 – 5891PhosphoserineCombined sources
Modified residuei592 – 5921PhosphoserineBy similarity
Modified residuei645 – 6451PhosphoserineCombined sources
Modified residuei648 – 6481PhosphoserineCombined sources
Modified residuei844 – 8441PhosphoserineCombined sources
Modified residuei938 – 9381PhosphoserineBy similarity
Modified residuei1113 – 11131PhosphoserineCombined sources
Modified residuei1119 – 11191PhosphoserineBy similarity
Isoform 2 (identifier: O35413-2)
Modified residuei148 – 1481Alanine amide

Post-translational modificationi

Ubiquitinated by CBL.By similarity

Keywords - PTMi

Amidation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO35413.
PRIDEiO35413.

PTM databases

iPTMnetiO35413.
PhosphoSiteiO35413.

Expressioni

Tissue specificityi

Expressed in brain; found in synapses in cerebellum.2 Publications

Interactioni

Subunit structurei

Interacts with ABL1/c-Abl, ABL2/v-Abl/Arg, ACTN, AKT1, CBL, PALLD and PAK1 (By similarity). Interacts with ABL, CBL, DNM1, DNM2, FLOT1, MLLT4/afadin, PTK2B/PYK2, SAPAP, SPTAN1, SYNJ1, SYNJ2, VCL/vinculin, and WASF. Interacts with PTPN12 and WASF1 via its SH3 domains; this interaction may mediate the partial PTPN12 and WASF1 translocation to focal adhesion sites.By similarity3 Publications

Protein-protein interaction databases

BioGridi250427. 5 interactions.
IntActiO35413. 2 interactions.
MINTiMINT-1216268.
STRINGi10116.ENSRNOP00000029182.

Structurei

3D structure databases

ProteinModelPortaliO35413.
SMRiO35413. Positions 963-1019, 1037-1095, 1139-1193.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini166 – 22762SoHoPROSITE-ProRule annotationAdd
BLAST
Domaini959 – 101860SH3 1PROSITE-ProRule annotationAdd
BLAST
Domaini1034 – 109562SH3 2PROSITE-ProRule annotationAdd
BLAST
Domaini1137 – 119660SH3 3PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi325 – 425101Ser-richAdd
BLAST
Compositional biasi735 – 74612His-richAdd
BLAST
Compositional biasi1027 – 10304Poly-Pro

Domaini

The first 2 SH3 domains are required for WASF1-binding. All 3 SH3 domains can bind independently to PTPN12.By similarity

Sequence similaritiesi

Contains 3 SH3 domains.PROSITE-ProRule annotation
Contains 1 SoHo domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiENOG410IPNR. Eukaryota.
ENOG410XNVJ. LUCA.
HOGENOMiHOG000294090.
HOVERGENiHBG108509.
InParanoidiO35413.
PhylomeDBiO35413.

Family and domain databases

InterProiIPR028516. ArgBP2.
IPR001452. SH3_domain.
IPR003127. SoHo_dom.
IPR007087. Znf_C2H2.
[Graphical view]
PANTHERiPTHR10663:SF207. PTHR10663:SF207. 1 hit.
PfamiPF00018. SH3_1. 2 hits.
PF14604. SH3_9. 1 hit.
PF02208. Sorb. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 3 hits.
SM00459. Sorb. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 3 hits.
PROSITEiPS50002. SH3. 3 hits.
PS50831. SOHO. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O35413-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNTDSGGCAR KRAAMSVTLT SVKRVQSSPN LLAAGRESHS PDSAWRSYNG
60 70 80 90 100
RNPETLNGDA TYSSLAAKGF RSVRPNLQDK KSPTQSHITI NGNSGGAVSP
110 120 130 140 150
VSYYQRPFSP SAYSLPASLN SSIIMPHGRS LDSAETYSQH AQSLDGTMGS
160 170 180 190 200
SIPLYRSSEE EKRVTVIKAP HYPGIGPVDE SGIPTAIRTT VDRPKDWYKT
210 220 230 240 250
MFKQIHMVHK PDEDTDMYNT PYTYNAGLYN SPYSAQSHPA AKTQTYRPLS
260 270 280 290 300
KSHSDNGTDA FKEATSPVPP PHVPPRPRDQ SSTEKHDWDP PDRKVDTRKF
310 320 330 340 350
RSEPRSIFEY EPGKSSILQH ERPVSVYQSS IDRSLERPSS SASMAGDFRK
360 370 380 390 400
RRKSEPAVGP PRGLGDHSSS RTSPGRADLP GSSSTFTTSF ISSSPSSPSR
410 420 430 440 450
AQGGDDSKMC PPLCSYSGLN GSPSSELECC GAYRRHLDVP QDSQRAITFK
460 470 480 490 500
NGWQMARQNA EIWSSTEEAV SPKIKSRSCD DLLNDDCGSF PDPKTKSESM
510 520 530 540 550
GSLLCDEGSK ESDPMTWTSP YIPEVCGNSR SRLKHRSAHN APGFLKMYKK
560 570 580 590 600
MHRINRKDLM NSEVICSVKS RILQYEKEQQ HRGLLHGWSQ SSTEEVPRDV
610 620 630 640 650
VPTRISEFEK LIQKSKSMPN LGDEMLSPVT LEPPQNGLCP KRRFSIESLL
660 670 680 690 700
EEETQVRHPS QGQRSCKSNT LVPIHIEVTS DEQPRTHMEF SDSDQDGVVS
710 720 730 740 750
DHSDNVHVER SSFCSESDFD HFSFTSSESF YGSSHHHHHH HHHHGHFISS
760 770 780 790 800
CKGRCPASYT RFTTMLKHER AKHENIDRPR RQDMDPGLSK LAFLVSPVPF
810 820 830 840 850
RRKKVLTPQK QTEQAKCKAS VVEALDSALK DICDQIKAEK RRGSLPDNSI
860 870 880 890 900
LHRLISELLP QIPKRNSSLN ALKRSPMHQP FHPLPQDGAI HCPLYQNDCG
910 920 930 940 950
RMPHSASFPD VDTTSSYHAQ DYGSVLSLQD HESPRSYSST LTDLGRSVSR
960 970 980 990 1000
ERRGTPEKEV KLPAKAVYDF KAQTSKELSF KKGDTVYILR KIDQNWYEGE
1010 1020 1030 1040 1050
HHGRVGIFPI SYVEKLTPPE KAQPARPPPP VQPGEIGEAI AKYNFNADTN
1060 1070 1080 1090 1100
VELSLRKGDR IILLKRVDQN WYEGKIPGTN RQGIFPVSYV EVVKRNTKGS
1110 1120 1130 1140 1150
EDYPDPPLPH SYSSDRIYSL SSNKPQRPVF SHENIQGGGE PFQALYNYTP
1160 1170 1180 1190
RNEDELELRE SDVVDVMEKC DDGWFVGTSR RTKFFGTFPG NYVKRL
Length:1,196
Mass (Da):134,057
Last modified:July 22, 2008 - v2
Checksum:i122E5B7B0534B855
GO
Isoform 2 (identifier: O35413-2) [UniParc]FASTAAdd to basket

Also known as: Sorbin

The sequence of this isoform differs from the canonical sequence as follows:
     1-181: Missing.
     182-185: GIPT → MISQ
     326-334: VYQSSIDRS → KPQAGRRKV
     335-1196: Missing.

Show »
Length:153
Mass (Da):17,709
Checksum:i0B0CE07EC7173125
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti265 – 2651T → P in AAB81527 (PubMed:10521485).Curated
Sequence conflicti354 – 3541S → V in AAK81861 (PubMed:11786189).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 181181Missing in isoform 2. 1 PublicationVSP_034812Add
BLAST
Alternative sequencei182 – 1854GIPT → MISQ in isoform 2. 1 PublicationVSP_034813
Alternative sequencei326 – 3349VYQSSIDRS → KPQAGRRKV in isoform 2. 1 PublicationVSP_034814
Alternative sequencei335 – 1196862Missing in isoform 2. 1 PublicationVSP_034815Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF026505 mRNA. Translation: AAB81527.1.
AF396458 mRNA. Translation: AAK81861.1.
PIRiT14108.
RefSeqiNP_446222.1. NM_053770.1.
UniGeneiRn.24612.

Genome annotation databases

GeneIDi114901.
KEGGirno:114901.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF026505 mRNA. Translation: AAB81527.1.
AF396458 mRNA. Translation: AAK81861.1.
PIRiT14108.
RefSeqiNP_446222.1. NM_053770.1.
UniGeneiRn.24612.

3D structure databases

ProteinModelPortaliO35413.
SMRiO35413. Positions 963-1019, 1037-1095, 1139-1193.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250427. 5 interactions.
IntActiO35413. 2 interactions.
MINTiMINT-1216268.
STRINGi10116.ENSRNOP00000029182.

PTM databases

iPTMnetiO35413.
PhosphoSiteiO35413.

Proteomic databases

PaxDbiO35413.
PRIDEiO35413.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi114901.
KEGGirno:114901.

Organism-specific databases

CTDi8470.
RGDi620061. Sorbs2.

Phylogenomic databases

eggNOGiENOG410IPNR. Eukaryota.
ENOG410XNVJ. LUCA.
HOGENOMiHOG000294090.
HOVERGENiHBG108509.
InParanoidiO35413.
PhylomeDBiO35413.

Miscellaneous databases

NextBioi618931.
PROiO35413.

Family and domain databases

InterProiIPR028516. ArgBP2.
IPR001452. SH3_domain.
IPR003127. SoHo_dom.
IPR007087. Znf_C2H2.
[Graphical view]
PANTHERiPTHR10663:SF207. PTHR10663:SF207. 1 hit.
PfamiPF00018. SH3_1. 2 hits.
PF14604. SH3_9. 1 hit.
PF02208. Sorb. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 3 hits.
SM00459. Sorb. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 3 hits.
PROSITEiPS50002. SH3. 3 hits.
PS50831. SOHO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "nArgBP2, a novel neural member of ponsin/ArgBP2/vinexin family that interacts with synapse-associated protein 90/postsynaptic density-95-associated protein (SAPAP)."
    Kawabe H., Hata Y., Takeuchi M., Ide N., Mizoguchi A., Takai Y.
    J. Biol. Chem. 274:30914-30918(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH SAPAP; VCL AND MLLT4, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION.
    Tissue: Brain.
  2. "Coding region of the sorbin gene in different species."
    Wahbi K., Magaud J.-P., Pansu D., Descroix-Vagne M.
    Peptides 22:2045-2053(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 182-354 (ISOFORM 2), AMIDATION AT ALA-148 (ISOFORM 2).
    Strain: Wistar.
  3. "Recruitment of Pyk2 and Cbl to lipid rafts mediates signals important for actin reorganization in growing neurites."
    Haglund K., Ivankovic-Dikic I., Shimokawa N., Kruh G.D., Dikic I.
    J. Cell Sci. 117:2557-2568(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FLOT1; PTK2B AND CBL, SUBCELLULAR LOCATION.
  4. "Involvement of palladin and alpha-actinin in targeting of the Abl/Arg kinase adaptor ArgBP2 to the actin cytoskeleton."
    Roenty M., Taivainen A., Moza M., Kruh G.D., Ehler E., Carpen O.
    Exp. Cell Res. 310:88-98(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. "The Abl/Arg substrate ArgBP2/nArgBP2 coordinates the function of multiple regulatory mechanisms converging on the actin cytoskeleton."
    Cestra G., Toomre D., Chang S., De Camilli P.
    Proc. Natl. Acad. Sci. U.S.A. 102:1731-1736(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH SYNJ1; SYNJ2; DNM1; DNM2; VCL; WASF; CBL; ABL; SPTAN1 AND PTK2B.
  6. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-27; SER-40; SER-143; SER-254; SER-343; SER-354; SER-397; SER-589; SER-645; SER-648; SER-844 AND SER-1113, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSRBS2_RAT
AccessioniPrimary (citable) accession number: O35413
Secondary accession number(s): Q923T8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: July 22, 2008
Last modified: May 11, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.