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Protein

Phospholipase D3

Gene

Pld3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probably involved in APP processing.By similarity

Catalytic activityi

A phosphatidylcholine + H2O = choline + a phosphatidate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei199 – 1991PROSITE-ProRule annotation
Active sitei201 – 2011PROSITE-ProRule annotation
Active sitei206 – 2061PROSITE-ProRule annotation

GO - Molecular functioni

  • N-acylphosphatidylethanolamine-specific phospholipase D activity Source: UniProtKB-EC
  • phospholipase D activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase D3 (EC:3.1.4.4)
Short name:
PLD 3
Alternative name(s):
Choline phosphatase 3
Phosphatidylcholine-hydrolyzing phospholipase D3
Schwannoma-associated protein 9
Short name:
SAM-9
Gene namesi
Name:Pld3
Synonyms:Sam9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1333782. Pld3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3838CytoplasmicSequence analysisAdd
BLAST
Transmembranei39 – 5921Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini60 – 488429LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 488488Phospholipase D3PRO_0000280328Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi132 – 1321N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Glycosylated.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiO35405.
MaxQBiO35405.
PaxDbiO35405.
PRIDEiO35405.

PTM databases

iPTMnetiO35405.
PhosphoSiteiO35405.

Expressioni

Tissue specificityi

Expressed at higher level in brain than in non-nervous tissue. Probably highly expressed in neurons. Expressed in mature neurons of the forebrain and appears to be turned on at late stages of neurogenesis. Expressed during late neuronal development in the forebrain.1 Publication

Gene expression databases

BgeeiO35405.
CleanExiMM_PLD3.
ExpressionAtlasiO35405. baseline and differential.
GenevisibleiO35405. MM.

Interactioni

Subunit structurei

Interacts with APP.By similarity

Protein-protein interaction databases

BioGridi202241. 1 interaction.
IntActiO35405. 1 interaction.
MINTiMINT-4104719.
STRINGi10090.ENSMUSP00000112942.

Structurei

3D structure databases

ProteinModelPortaliO35405.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini194 – 22128PLD phosphodiesterase 1PROSITE-ProRule annotationAdd
BLAST
Domaini409 – 43527PLD phosphodiesterase 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the phospholipase D family.Curated
Contains 2 PLD phosphodiesterase domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3603. Eukaryota.
ENOG410XQZ4. LUCA.
GeneTreeiENSGT00390000009798.
HOGENOMiHOG000293407.
HOVERGENiHBG052880.
InParanoidiO35405.
KOiK16860.
OMAiPRFLSTY.
OrthoDBiEOG70KGP9.
PhylomeDBiO35405.
TreeFamiTF313378.

Family and domain databases

InterProiIPR032803. PLDc_3.
IPR001736. PLipase_D/transphosphatidylase.
[Graphical view]
PfamiPF00614. PLDc. 1 hit.
PF13918. PLDc_3. 1 hit.
[Graphical view]
SMARTiSM00155. PLDc. 2 hits.
[Graphical view]
PROSITEiPS50035. PLD. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35405-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPKLMYQEL KVPVEEPAGE LPLNEIEAWK AAEKKARWVL LVLILAVVGF
60 70 80 90 100
GALMTQLFLW EYGDLHLFGP NQRPAPCYDP CEAVLVESIP EGLEFPNATT
110 120 130 140 150
SNPSTSQAWL GLLAGAHSSL DIASFYWTLT NNDTHTQEPS AQQGEEVLQQ
160 170 180 190 200
LQALAPRGVK VRIAVSKPNG PLADLQSLLQ SGAQVRMVDM QKLTHGVLHT
210 220 230 240 250
KFWVVDQTHF YLGSANMDWR SLTQVKELGV VMYNCSCLAR DLTKIFEAYW
260 270 280 290 300
FLGQAGSSIP STWPRSFDTR YNQETPMEIC LNGTPALAYL ASAPPPLCPS
310 320 330 340 350
GRTPDLKALL NVVDSARSFI YIAVMNYLPT MEFSHPRRFW PAIDDGLRRA
360 370 380 390 400
AYERGVKVRL LISCWGHSDP SMRSFLLSLA ALHDNHTHSD IQVKLFVVPT
410 420 430 440 450
DESQARIPYA RVNHNKYMVT ERASYIGTSN WSGSYFTETA GTSLLVTQNG
460 470 480
HGGLRSQLEA VFLRDWESPY SHDLDTSANS VGNACRLL
Length:488
Mass (Da):54,389
Last modified:January 1, 1998 - v1
Checksum:iC7F0184EB09D7573
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti18 – 181A → G in BAE32053 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF026124 mRNA. Translation: AAC73069.1.
AK153508 mRNA. Translation: BAE32053.1.
BC076586 mRNA. Translation: AAH76586.1.
CCDSiCCDS21025.1.
RefSeqiNP_001304284.1. NM_001317355.1.
NP_035246.1. NM_011116.2.
XP_006539705.1. XM_006539642.2.
XP_006539706.1. XM_006539643.2.
UniGeneiMm.6483.

Genome annotation databases

EnsembliENSMUST00000117095; ENSMUSP00000113820; ENSMUSG00000003363.
ENSMUST00000117611; ENSMUSP00000112942; ENSMUSG00000003363.
GeneIDi18807.
KEGGimmu:18807.
UCSCiuc009fwm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF026124 mRNA. Translation: AAC73069.1.
AK153508 mRNA. Translation: BAE32053.1.
BC076586 mRNA. Translation: AAH76586.1.
CCDSiCCDS21025.1.
RefSeqiNP_001304284.1. NM_001317355.1.
NP_035246.1. NM_011116.2.
XP_006539705.1. XM_006539642.2.
XP_006539706.1. XM_006539643.2.
UniGeneiMm.6483.

3D structure databases

ProteinModelPortaliO35405.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202241. 1 interaction.
IntActiO35405. 1 interaction.
MINTiMINT-4104719.
STRINGi10090.ENSMUSP00000112942.

PTM databases

iPTMnetiO35405.
PhosphoSiteiO35405.

Proteomic databases

EPDiO35405.
MaxQBiO35405.
PaxDbiO35405.
PRIDEiO35405.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000117095; ENSMUSP00000113820; ENSMUSG00000003363.
ENSMUST00000117611; ENSMUSP00000112942; ENSMUSG00000003363.
GeneIDi18807.
KEGGimmu:18807.
UCSCiuc009fwm.1. mouse.

Organism-specific databases

CTDi23646.
MGIiMGI:1333782. Pld3.

Phylogenomic databases

eggNOGiKOG3603. Eukaryota.
ENOG410XQZ4. LUCA.
GeneTreeiENSGT00390000009798.
HOGENOMiHOG000293407.
HOVERGENiHBG052880.
InParanoidiO35405.
KOiK16860.
OMAiPRFLSTY.
OrthoDBiEOG70KGP9.
PhylomeDBiO35405.
TreeFamiTF313378.

Miscellaneous databases

ChiTaRSiPld3. mouse.
NextBioi295120.
PROiO35405.
SOURCEiSearch...

Gene expression databases

BgeeiO35405.
CleanExiMM_PLD3.
ExpressionAtlasiO35405. baseline and differential.
GenevisibleiO35405. MM.

Family and domain databases

InterProiIPR032803. PLDc_3.
IPR001736. PLipase_D/transphosphatidylase.
[Graphical view]
PfamiPF00614. PLDc. 1 hit.
PF13918. PLDc_3. 1 hit.
[Graphical view]
SMARTiSM00155. PLDc. 2 hits.
[Graphical view]
PROSITEiPS50035. PLD. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of a novel murine phospholipase D homolog coincides with late neuronal development in the forebrain."
    Pedersen K.M., Finsen B., Celis J.E., Jensen N.A.
    J. Biol. Chem. 273:31494-31504(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: C57BL/6 X DBA/2.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Bone marrow.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Eye.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Spleen and Testis.

Entry informationi

Entry nameiPLD3_MOUSE
AccessioniPrimary (citable) accession number: O35405
Secondary accession number(s): Q3U5M5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: January 1, 1998
Last modified: March 16, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.