Reviewed, UniProtKB/Swiss-Prot O35397 (CASP6_RAT)

Names and origin

Protein names

Recommended name:
    Caspase-6
      Short name=CASP-6
    EC=3.4.22.59
Alternative name(s):
    Apoptotic protease Mch-2
Cleaved into the following 2 chains:
    1- Recommended name:
            Caspase-6 subunit p18
    2- Recommended name:
            Caspase-6 subunit p11

Gene names

Name:	Casp6
Synonyms:	Mch2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death By similarity.
Catalytic activity	Strict requirement for Asp at position P1 and has a preferred cleavage sequence of Val-Glu-His-Asp-|-.
Enzyme regulation	Activation is suppressed by phosphorylation at Ser-240 By similarity.
Subunit structure	Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 11 kDa (p11) subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits By similarity.
Sequence similarities	Belongs to the peptidase C14A family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm
Molecular function	Hydrolase Protease Thiol protease
PTM	Phosphoprotein Zymogen
Gene Ontology (GO)
Biological process	acute inflammatory response to non-antigenic stimulus Inferred from direct assay. Source: RGD apoptosis Traceable author statement. Source: RGD lens development in camera-type eye Inferred from expression pattern. Source: RGD positive regulation of neuron apoptosis Inferred from mutant phenotype. Source: RGD positive regulation of retinal cell programmed cell death Inferred from mutant phenotype. Source: RGD proteolysis Inferred from electronic annotation. Source: InterPro response to cytokine stimulus Inferred from direct assay. Source: RGD response to estradiol stimulus Inferred from expression pattern. Source: RGD response to gamma radiation Inferred from expression pattern. Source: RGD response to glucose stimulus Inferred from mutant phenotype. Source: RGD response to heat Inferred from expression pattern. Source: RGD response to hydrogen peroxide Inferred from direct assay. Source: RGD response to iron ion Inferred from mutant phenotype. Source: RGD response to organic nitrogen Inferred from expression pattern. Source: RGD response to testosterone stimulus Inferred from expression pattern. Source: RGD
Cellular component	cytosol Inferred from direct assay. Source: RGD nucleus Inferred from direct assay. Source: RGD
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 5	5	By similarity		PRO_0000233177
	Chain	6 – 162	157	Caspase-6 subunit p18 By similarity		PRO_0000233178
	Propeptide	163 – 177	15	By similarity		PRO_0000233179
	Chain	178 – 277	100	Caspase-6 subunit p11 By similarity		PRO_0000233180
Sites
	Active site	104	1	By similarity
	Active site	146	1	By similarity
Amino acid modifications
	Modified residue	240	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O35397-1 [UniParc]. Last modified November 1, 1998. Version 2. Checksum: 69D999E729B3BDE2	FASTA	277	31,556
	10 20 30 40 50 60 MTETDGFYRS REVLDPAEQY KMDHKRRGTA LIFNHERFFW HLALPERRGT NADRDNPTRR 70 80 90 100 110 120 FSELGFEVKC FNDLRAEELL LKIHEVSTSS HVDADCFLCV FLSHGEGNHI YAYDAKIEIQ 130 140 150 160 170 180 TLTGLFKGDK CQSLVGKPKI FIIQACRGSQ HDVPLVPLDV VDHQTDKLDD NVTQVDAASV 190 200 210 220 230 240 YTLPAGADFL MCYSVAEGYY SHRETVNGSW YIQDLSEMLA RHGSSLEFTE LLTLVNRKVS 250 260 270 QRRVDFCKDP GAIGKKQVPC FASMLTKKLH FCPKPSK

References

[1]	"Identification of gene family of caspases in rat kidney and altered expression in ischemia-reperfusion injury." Kaushal G.P., Singh A.B., Shah S.V. Am. J. Physiol. 274:F587-F595(1998) [PubMed: 9530276] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Kidney cortex.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF025670 mRNA. Translation: AAC25433.1.
IPI	IPI00464786.
UniGene	Rn.88160
3D structure databases
HSSP	HSSP built from PDB template 1NME based on UniProtKB P42574.
ModBase	Search...
Protein-protein interaction databases
STRING	O35397.
Protein family/group databases
MEROPS	C14.005.
PTM databases
PhosphoSite	O35397.
Genome annotation databases
Ensembl	ENSRNOT00000013049; ENSRNOP00000013049; ENSRNOG00000009508; Rattus norvegicus. [Genome view]
Organism-specific databases
RGD	70967. Casp6.
Phylogenomic databases
HOVERGEN	O35397.
Enzyme and pathway databases
BRENDA	3.4.22.59. 248.
Gene expression databases
ArrayExpress	O35397.
Genevestigator	O35397.
GermOnline	ENSRNOG00000009508. Rattus norvegicus.
Family and domain databases
InterPro	IPR015469. Caspase_6. IPR011600. Pept_C14_cat. IPR001309. Pept_C14_ICE_p20. IPR016129. Pept_C14_ICE_p20_AS. IPR002138. Pept_C14_p10. IPR002398. Pept_C14_p45. IPR015917. Pept_C14_p45_core. [Graphical view]
PANTHER	PTHR10454:SF29. Casp6. 1 hit. PTHR10454. Pept_C14_p45. 1 hit.
Pfam	PF00656. Peptidase_C14. 1 hit. [Graphical view]
PRINTS	PR00376. IL1BCENZYME.
SMART	SM00115. CASc. 1 hit. [Graphical view]
PROSITE	PS01122. CASPASE_CYS. 1 hit. PS01121. CASPASE_HIS. 1 hit. PS50207. CASPASE_P10. 1 hit. PS50208. CASPASE_P20. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CASP6_RAT

Accession

Primary (citable) accession number: O35397

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	November 1, 1998
Last modified:	October 13, 2009
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)