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Protein

Caspase-6

Gene

Casp6

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death (By similarity).By similarity

Catalytic activityi

Strict requirement for Asp at position P1 and has a preferred cleavage sequence of Val-Glu-His-Asp-|-.

Enzyme regulationi

Activation is suppressed by phosphorylation at Ser-240.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei104 – 1041By similarity
Active sitei146 – 1461By similarity

GO - Molecular functioni

GO - Biological processi

  • acute inflammatory response to non-antigenic stimulus Source: RGD
  • apoptotic process Source: RGD
  • lens development in camera-type eye Source: RGD
  • positive regulation of apoptotic process Source: RGD
  • positive regulation of neuron apoptotic process Source: RGD
  • positive regulation of retinal cell programmed cell death Source: RGD
  • response to cytokine Source: RGD
  • response to estradiol Source: RGD
  • response to gamma radiation Source: RGD
  • response to glucose Source: RGD
  • response to heat Source: RGD
  • response to hydrogen peroxide Source: RGD
  • response to iron ion Source: RGD
  • response to metal ion Source: RGD
  • response to organic substance Source: RGD
  • response to organonitrogen compound Source: RGD
  • response to testosterone Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Apoptosis

Protein family/group databases

MEROPSiC14.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Caspase-6 (EC:3.4.22.59)
Short name:
CASP-6
Alternative name(s):
Apoptotic protease Mch-2
Cleaved into the following 2 chains:
Gene namesi
Name:Casp6
Synonyms:Mch2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi70967. Casp6.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • cytosol Source: RGD
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 55By similarityPRO_0000233177
Chaini6 – 162157Caspase-6 subunit p18By similarityPRO_0000233178Add
BLAST
Propeptidei163 – 17715By similarityPRO_0000233179Add
BLAST
Chaini178 – 277100Caspase-6 subunit p11By similarityPRO_0000233180Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei62 – 621PhosphoserineBy similarity
Modified residuei240 – 2401Phosphoserine; by NUAK1By similarity

Post-translational modificationi

Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.By similarity

Keywords - PTMi

Phosphoprotein, Zymogen

Proteomic databases

PaxDbiO35397.
PRIDEiO35397.

PTM databases

PhosphoSiteiO35397.

Interactioni

Subunit structurei

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013049.

Structurei

3D structure databases

ProteinModelPortaliO35397.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C14A family.Curated

Phylogenomic databases

eggNOGiKOG3573. Eukaryota.
ENOG410ZQIE. LUCA.
HOGENOMiHOG000231878.
HOVERGENiHBG050802.
InParanoidiO35397.
PhylomeDBiO35397.

Family and domain databases

Gene3Di3.40.50.1460. 1 hit.
InterProiIPR029030. Caspase-like_dom.
IPR033139. Caspase_cys_AS.
IPR016129. Caspase_his_AS.
IPR002138. Pept_C14_p10.
IPR001309. Pept_C14_p20.
IPR015917. Pept_C14A.
[Graphical view]
PRINTSiPR00376. IL1BCENZYME.
SMARTiSM00115. CASc. 1 hit.
[Graphical view]
SUPFAMiSSF52129. SSF52129. 1 hit.
PROSITEiPS01122. CASPASE_CYS. 1 hit.
PS01121. CASPASE_HIS. 1 hit.
PS50207. CASPASE_P10. 1 hit.
PS50208. CASPASE_P20. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35397-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTETDGFYRS REVLDPAEQY KMDHKRRGTA LIFNHERFFW HLALPERRGT
60 70 80 90 100
NADRDNPTRR FSELGFEVKC FNDLRAEELL LKIHEVSTSS HVDADCFLCV
110 120 130 140 150
FLSHGEGNHI YAYDAKIEIQ TLTGLFKGDK CQSLVGKPKI FIIQACRGSQ
160 170 180 190 200
HDVPLVPLDV VDHQTDKLDD NVTQVDAASV YTLPAGADFL MCYSVAEGYY
210 220 230 240 250
SHRETVNGSW YIQDLSEMLA RHGSSLEFTE LLTLVNRKVS QRRVDFCKDP
260 270
GAIGKKQVPC FASMLTKKLH FCPKPSK
Length:277
Mass (Da):31,556
Last modified:November 1, 1998 - v2
Checksum:i69D999E729B3BDE2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF025670 mRNA. Translation: AAC25433.1.
UniGeneiRn.88160.

Genome annotation databases

UCSCiRGD:70967. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF025670 mRNA. Translation: AAC25433.1.
UniGeneiRn.88160.

3D structure databases

ProteinModelPortaliO35397.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013049.

Protein family/group databases

MEROPSiC14.005.

PTM databases

PhosphoSiteiO35397.

Proteomic databases

PaxDbiO35397.
PRIDEiO35397.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:70967. rat.

Organism-specific databases

RGDi70967. Casp6.

Phylogenomic databases

eggNOGiKOG3573. Eukaryota.
ENOG410ZQIE. LUCA.
HOGENOMiHOG000231878.
HOVERGENiHBG050802.
InParanoidiO35397.
PhylomeDBiO35397.

Miscellaneous databases

PROiO35397.

Family and domain databases

Gene3Di3.40.50.1460. 1 hit.
InterProiIPR029030. Caspase-like_dom.
IPR033139. Caspase_cys_AS.
IPR016129. Caspase_his_AS.
IPR002138. Pept_C14_p10.
IPR001309. Pept_C14_p20.
IPR015917. Pept_C14A.
[Graphical view]
PRINTSiPR00376. IL1BCENZYME.
SMARTiSM00115. CASc. 1 hit.
[Graphical view]
SUPFAMiSSF52129. SSF52129. 1 hit.
PROSITEiPS01122. CASPASE_CYS. 1 hit.
PS01121. CASPASE_HIS. 1 hit.
PS50207. CASPASE_P10. 1 hit.
PS50208. CASPASE_P20. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCASP6_RAT
AccessioniPrimary (citable) accession number: O35397
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: November 1, 1998
Last modified: July 6, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.