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Protein

Prenylated Rab acceptor protein 1

Gene

Rabac1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

General Rab protein regulator required for vesicle formation from the Golgi complex. May control vesicle docking and fusion by mediating the action of Rab GTPases to the SNARE complexes. In addition it inhibits the removal of Rab GTPases from the membrane by GDI1.3 Publications

GO - Molecular functioni

  • proline-rich region binding Source: RGD
  • protein binding, bridging Source: RGD
  • protein C-terminus binding Source: RGD

GO - Biological processi

  • vesicle-mediated transport Source: RGD
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Prenylated Rab acceptor protein 1
Alternative name(s):
PRA1 family protein 1
Gene namesi
Name:Rabac1
Synonyms:Pra1, Praf1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi621002. Rabac1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 78CytoplasmicBy similarityAdd BLAST78
Transmembranei79 – 94HelicalBy similarityAdd BLAST16
Transmembranei95 – 112HelicalBy similarityAdd BLAST18
Topological domaini113 – 131CytoplasmicBy similarityAdd BLAST19
Transmembranei132 – 148HelicalBy similarityAdd BLAST17
Transmembranei149 – 165HelicalBy similarityAdd BLAST17
Topological domaini166 – 185CytoplasmicBy similarityAdd BLAST20

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi70N → T: Retained in endoplasmic reticulum, no interaction with RAB3A or VAMP2. 1 Publication1
Mutagenesisi73Y → A: Retained in endoplasmic reticulum, Golgi and tubular structures, no interaction with RAB3A. 1 Publication1
Mutagenesisi76S → A or V: Increased interaction with RAB3A or VAMP2, Golgi condensation. 1 Publication1
Mutagenesisi77N → A: No effect. 1 Publication1
Mutagenesisi78Y → A: Retained in endoplasmic reticulum, no interaction with RAB3A or VAMP2. 1 Publication1
Mutagenesisi154W → A: No effect. 1 Publication1
Mutagenesisi161V → A: Increased interaction with RAB3A or VAMP2, Golgi condensation. 1 Publication1
Mutagenesisi166H → A: Retained in endoplasmic reticulum, Golgi and tubular structures, no interaction with RAB3A. 1 Publication1
Mutagenesisi176D → A: Retained in endoplasmic reticulum. 1 Publication1
Mutagenesisi178E → A: Retained in endoplasmic reticulum. 1 Publication1
Mutagenesisi179E → A: Retained in endoplasmic reticulum. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002208801 – 185Prenylated Rab acceptor protein 1Add BLAST185

Proteomic databases

PaxDbiO35394.
PRIDEiO35394.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSRNOG00000020233.
GenevisibleiO35394. RN.

Interactioni

Subunit structurei

Homodimers (By similarity). Interacts specifically with both prenylated Rab proteins (including RAB3A and RAB1), and VAMP2 (synaptobrevin-2), in an exclusive way. Interacts with NDRG1 (By similarity). Interacts with free GDI1 in the absence of Rab proteins. Also interacts with PCLO.By similarity2 Publications

GO - Molecular functioni

  • proline-rich region binding Source: RGD
  • protein binding, bridging Source: RGD
  • protein C-terminus binding Source: RGD

Protein-protein interaction databases

IntActiO35394. 2 interactors.
MINTiMINT-4577486.
STRINGi10116.ENSRNOP00000027435.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni30 – 54Required for interaction with prenylated RAB3A and VAMP2Add BLAST25
Regioni165 – 185Required for interaction with GDI11 PublicationAdd BLAST21
Regioni175 – 185HomodimerizationBy similarityAdd BLAST11
Regioni175 – 185Required for interaction with prenylated RAB3A and VAMP2Add BLAST11

Sequence similaritiesi

Belongs to the PRA1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3142. Eukaryota.
COG5130. LUCA.
GeneTreeiENSGT00390000010549.
HOGENOMiHOG000230621.
HOVERGENiHBG053660.
InParanoidiO35394.
KOiK20359.
OMAiVVFIGWI.
OrthoDBiEOG091G0M2S.
PhylomeDBiO35394.
TreeFamiTF324857.

Family and domain databases

InterProiIPR004895. Prenylated_rab_accept_PRA1.
[Graphical view]
PfamiPF03208. PRA1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35394-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAQKDQQKD AEVEGLSATT LLPKLIPSGA GREWLERRRA TIRPWGTFVD
60 70 80 90 100
QQRFSRPRNV GELCQRLVRN VEYYQSNYVF VFLGLILYCV VTSPMLLVAL
110 120 130 140 150
AVFFGACYIL YLRTLQSKLV LFGREVSPAH QYALAGGVSF PFFWLAGAGS
160 170 180
AVFWVLGATL VLIGSHAAFH QIEPADGEEL QMEPV
Length:185
Mass (Da):20,643
Last modified:January 1, 1998 - v1
Checksum:iDA6341AE66F5C2F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF025506 mRNA. Translation: AAB81721.1.
BC086387 mRNA. Translation: AAH86387.1.
RefSeqiNP_113962.1. NM_031774.1.
UniGeneiRn.25604.

Genome annotation databases

EnsembliENSRNOT00000027435; ENSRNOP00000027435; ENSRNOG00000020233.
GeneIDi83583.
KEGGirno:83583.
UCSCiRGD:621002. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF025506 mRNA. Translation: AAB81721.1.
BC086387 mRNA. Translation: AAH86387.1.
RefSeqiNP_113962.1. NM_031774.1.
UniGeneiRn.25604.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO35394. 2 interactors.
MINTiMINT-4577486.
STRINGi10116.ENSRNOP00000027435.

Proteomic databases

PaxDbiO35394.
PRIDEiO35394.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000027435; ENSRNOP00000027435; ENSRNOG00000020233.
GeneIDi83583.
KEGGirno:83583.
UCSCiRGD:621002. rat.

Organism-specific databases

CTDi10567.
RGDi621002. Rabac1.

Phylogenomic databases

eggNOGiKOG3142. Eukaryota.
COG5130. LUCA.
GeneTreeiENSGT00390000010549.
HOGENOMiHOG000230621.
HOVERGENiHBG053660.
InParanoidiO35394.
KOiK20359.
OMAiVVFIGWI.
OrthoDBiEOG091G0M2S.
PhylomeDBiO35394.
TreeFamiTF324857.

Miscellaneous databases

PROiO35394.

Gene expression databases

BgeeiENSRNOG00000020233.
GenevisibleiO35394. RN.

Family and domain databases

InterProiIPR004895. Prenylated_rab_accept_PRA1.
[Graphical view]
PfamiPF03208. PRA1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRAF1_RAT
AccessioniPrimary (citable) accession number: O35394
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

In contrast to the mouse ortholog, it does not interact with the Ras-like GTPases RAC1 and RHOA.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.