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Protein

Prenylated Rab acceptor protein 1

Gene

Rabac1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

General Rab protein regulator required for vesicle formation from the Golgi complex. May control vesicle docking and fusion by mediating the action of Rab GTPases to the SNARE complexes. In addition it inhibits the removal of Rab GTPases from the membrane by GDI1.3 Publications

GO - Molecular functioni

  • proline-rich region binding Source: RGD
  • protein binding, bridging Source: RGD
  • protein C-terminus binding Source: RGD

GO - Biological processi

  • vesicle-mediated transport Source: RGD
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Prenylated Rab acceptor protein 1
Alternative name(s):
PRA1 family protein 1
Gene namesi
Name:Rabac1
Synonyms:Pra1, Praf1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi621002. Rabac1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7878CytoplasmicBy similarityAdd
BLAST
Transmembranei79 – 9416HelicalBy similarityAdd
BLAST
Transmembranei95 – 11218HelicalBy similarityAdd
BLAST
Topological domaini113 – 13119CytoplasmicBy similarityAdd
BLAST
Transmembranei132 – 14817HelicalBy similarityAdd
BLAST
Transmembranei149 – 16517HelicalBy similarityAdd
BLAST
Topological domaini166 – 18520CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi70 – 701N → T: Retained in endoplasmic reticulum, no interaction with RAB3A or VAMP2. 1 Publication
Mutagenesisi73 – 731Y → A: Retained in endoplasmic reticulum, Golgi and tubular structures, no interaction with RAB3A. 1 Publication
Mutagenesisi76 – 761S → A or V: Increased interaction with RAB3A or VAMP2, Golgi condensation. 1 Publication
Mutagenesisi77 – 771N → A: No effect. 1 Publication
Mutagenesisi78 – 781Y → A: Retained in endoplasmic reticulum, no interaction with RAB3A or VAMP2. 1 Publication
Mutagenesisi154 – 1541W → A: No effect. 1 Publication
Mutagenesisi161 – 1611V → A: Increased interaction with RAB3A or VAMP2, Golgi condensation. 1 Publication
Mutagenesisi166 – 1661H → A: Retained in endoplasmic reticulum, Golgi and tubular structures, no interaction with RAB3A. 1 Publication
Mutagenesisi176 – 1761D → A: Retained in endoplasmic reticulum. 1 Publication
Mutagenesisi178 – 1781E → A: Retained in endoplasmic reticulum. 1 Publication
Mutagenesisi179 – 1791E → A: Retained in endoplasmic reticulum. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 185185Prenylated Rab acceptor protein 1PRO_0000220880Add
BLAST

Proteomic databases

PaxDbiO35394.
PRIDEiO35394.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

GenevisibleiO35394. RN.

Interactioni

Subunit structurei

Homodimers (By similarity). Interacts specifically with both prenylated Rab proteins (including RAB3A and RAB1), and VAMP2 (synaptobrevin-2), in an exclusive way. Interacts with NDRG1 (By similarity). Interacts with free GDI1 in the absence of Rab proteins. Also interacts with PCLO.By similarity2 Publications

GO - Molecular functioni

  • proline-rich region binding Source: RGD
  • protein binding, bridging Source: RGD
  • protein C-terminus binding Source: RGD

Protein-protein interaction databases

IntActiO35394. 2 interactions.
MINTiMINT-4577486.
STRINGi10116.ENSRNOP00000027435.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni30 – 5425Required for interaction with prenylated RAB3A and VAMP2Add
BLAST
Regioni165 – 18521Required for interaction with GDI1Add
BLAST
Regioni175 – 18511HomodimerizationBy similarityAdd
BLAST
Regioni175 – 18511Required for interaction with prenylated RAB3A and VAMP2Add
BLAST

Sequence similaritiesi

Belongs to the PRA1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3142. Eukaryota.
COG5130. LUCA.
GeneTreeiENSGT00390000010549.
HOGENOMiHOG000230621.
HOVERGENiHBG053660.
InParanoidiO35394.
OMAiVVFIGWI.
OrthoDBiEOG789CCJ.
PhylomeDBiO35394.
TreeFamiTF324857.

Family and domain databases

InterProiIPR004895. Prenylated_rab_accept_PRA1.
[Graphical view]
PfamiPF03208. PRA1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35394-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAQKDQQKD AEVEGLSATT LLPKLIPSGA GREWLERRRA TIRPWGTFVD
60 70 80 90 100
QQRFSRPRNV GELCQRLVRN VEYYQSNYVF VFLGLILYCV VTSPMLLVAL
110 120 130 140 150
AVFFGACYIL YLRTLQSKLV LFGREVSPAH QYALAGGVSF PFFWLAGAGS
160 170 180
AVFWVLGATL VLIGSHAAFH QIEPADGEEL QMEPV
Length:185
Mass (Da):20,643
Last modified:January 1, 1998 - v1
Checksum:iDA6341AE66F5C2F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF025506 mRNA. Translation: AAB81721.1.
BC086387 mRNA. Translation: AAH86387.1.
RefSeqiNP_113962.1. NM_031774.1.
UniGeneiRn.25604.

Genome annotation databases

EnsembliENSRNOT00000027435; ENSRNOP00000027435; ENSRNOG00000020233.
GeneIDi83583.
KEGGirno:83583.
UCSCiRGD:621002. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF025506 mRNA. Translation: AAB81721.1.
BC086387 mRNA. Translation: AAH86387.1.
RefSeqiNP_113962.1. NM_031774.1.
UniGeneiRn.25604.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO35394. 2 interactions.
MINTiMINT-4577486.
STRINGi10116.ENSRNOP00000027435.

Proteomic databases

PaxDbiO35394.
PRIDEiO35394.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000027435; ENSRNOP00000027435; ENSRNOG00000020233.
GeneIDi83583.
KEGGirno:83583.
UCSCiRGD:621002. rat.

Organism-specific databases

CTDi10567.
RGDi621002. Rabac1.

Phylogenomic databases

eggNOGiKOG3142. Eukaryota.
COG5130. LUCA.
GeneTreeiENSGT00390000010549.
HOGENOMiHOG000230621.
HOVERGENiHBG053660.
InParanoidiO35394.
OMAiVVFIGWI.
OrthoDBiEOG789CCJ.
PhylomeDBiO35394.
TreeFamiTF324857.

Miscellaneous databases

PROiO35394.

Gene expression databases

GenevisibleiO35394. RN.

Family and domain databases

InterProiIPR004895. Prenylated_rab_accept_PRA1.
[Graphical view]
PfamiPF03208. PRA1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of a dual prenylated Rab and VAMP2 receptor."
    Martincic I., Peralta M.E., Ngsee J.K.
    J. Biol. Chem. 272:26991-26998(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.
  3. "PRA1 inhibits the extraction of membrane-bound rab GTPase by GDI1."
    Hutt D.M., da Silva L.F., Chang L.-H., Prosser D.C., Ngsee J.K.
    J. Biol. Chem. 275:18511-18519(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH GDI1.
  4. "Disruption of Golgi morphology and trafficking in cells expressing mutant prenylated rab acceptor-1."
    Gougeon P.-Y., Prosser D.C., Da-Silva L.F., Ngsee J.K.
    J. Biol. Chem. 277:36408-36414(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ASN-70; TYR-73; SER-76; ASN-77; TYR-78; TRP-154; VAL-161 AND HIS-166, FUNCTION, SUBCELLULAR LOCATION.
  5. Cited for: MUTAGENESIS OF ASP-176; GLU-178 AND GLU-179, SUBCELLULAR LOCATION.
  6. Cited for: INTERACTION WITH PCLO, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiPRAF1_RAT
AccessioniPrimary (citable) accession number: O35394
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: January 1, 1998
Last modified: June 8, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

In contrast to the mouse ortholog, it does not interact with the Ras-like GTPases RAC1 and RHOA.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.