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O35393

- EFNB3_MOUSE

UniProt

O35393 - EFNB3_MOUSE

Protein

Ephrin-B3

Gene

Efnb3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. May play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons.1 Publication

    GO - Molecular functioni

    1. ephrin receptor binding Source: RefGenome

    GO - Biological processi

    1. adult walking behavior Source: MGI
    2. axon choice point recognition Source: MGI
    3. axon guidance Source: MGI
    4. ephrin receptor signaling pathway Source: RefGenome

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Differentiation, Neurogenesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ephrin-B3
    Gene namesi
    Name:Efnb3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:109196. Efnb3.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: RefGenome

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Chaini28 – 340313Ephrin-B3PRO_0000008396Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi62 ↔ 104PROSITE-ProRule annotation
    Disulfide bondi92 ↔ 156PROSITE-ProRule annotation
    Glycosylationi210 – 2101N-linked (GlcNAc...)1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiO35393.
    PaxDbiO35393.
    PRIDEiO35393.

    PTM databases

    PhosphoSiteiO35393.

    Expressioni

    Tissue specificityi

    Expressed on lateral floor plate cells, specifically on commissural axon segments that have passed through the floor plate. Expressed in cells of the retinal ganglion cell layer during retinal axon guidance to the optic disk.

    Developmental stagei

    Expressed in the floor plate throughout the period of commissural axon pathfinding.

    Gene expression databases

    ArrayExpressiO35393.
    BgeeiO35393.
    CleanExiMM_EFNB3.
    GenevestigatoriO35393.

    Interactioni

    Subunit structurei

    Interacts with GRIP1 and GRIP2.By similarity

    Protein-protein interaction databases

    DIPiDIP-44833N.
    IntActiO35393. 3 interactions.
    MINTiMINT-1793420.
    STRINGi10090.ENSMUSP00000004036.

    Structurei

    Secondary structure

    1
    340
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi44 – 463
    Beta strandi57 – 626
    Beta strandi80 – 845
    Helixi87 – 904
    Beta strandi99 – 1035
    Beta strandi107 – 1093
    Beta strandi111 – 1199
    Beta strandi134 – 1396
    Turni145 – 1495
    Helixi155 – 1584
    Beta strandi162 – 1665

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3D12X-ray3.00B/E29-169[»]
    ProteinModelPortaliO35393.
    SMRiO35393. Positions 29-169.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO35393.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini28 – 227200ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini249 – 34092CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei228 – 24821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini28 – 167140Ephrin RBDPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi338 – 3403PDZ-bindingSequence Analysis

    Sequence similaritiesi

    Belongs to the ephrin family.PROSITE-ProRule annotation
    Contains 1 ephrin RBD (ephrin receptor-binding) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG268537.
    HOGENOMiHOG000220931.
    HOVERGENiHBG051448.
    InParanoidiO35393.
    KOiK05463.
    OMAiPGKENMP.
    OrthoDBiEOG7288S5.
    PhylomeDBiO35393.

    Family and domain databases

    Gene3Di2.60.40.420. 1 hit.
    InterProiIPR008972. Cupredoxin.
    IPR001799. Ephrin.
    IPR019765. Ephrin_CS.
    [Graphical view]
    PANTHERiPTHR11304. PTHR11304. 1 hit.
    PfamiPF00812. Ephrin. 1 hit.
    [Graphical view]
    PRINTSiPR01347. EPHRIN.
    ProDomiPD002533. Ephrin. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF49503. SSF49503. 1 hit.
    PROSITEiPS01299. EPHRIN_RBD_1. 1 hit.
    PS51551. EPHRIN_RBD_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O35393-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGAPHFGPGG VQVGALLLLG FAGLVSGLSL EPVYWNSANK RFQAEGGYVL    50
    YPQIGDRLDL LCPRARPPGP HSSPSYEFYK LYLVEGAQGR RCEAPPAPNL 100
    LLTCDRPDLD LRFTIKFQEY SPNLWGHEFR SHHDYYIIAT SDGTREGLES 150
    LQGGVCLTRG MKVLLRVGQS PRGGAVPRKP VSEMPMERDR GAAHSAEPGR 200
    DTIPGDPSSN ATSRGAEGPL PPPSMPAVAG AAGGMALLLL GVAGAGGAMC 250
    WRRRRAKPSE SRHPGPGSFG RGGSLGLGGG GGMGPREAEP GELGIALRGG 300
    GTADPPFCPH YEKVSGDYGH PVYIVQDGPP QSPPNIYYKV 340
    Length:340
    Mass (Da):35,885
    Last modified:January 1, 1998 - v1
    Checksum:i52F3D58FD209A6B8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF025288 mRNA. Translation: AAC53537.1.
    BC052001 mRNA. Translation: AAH52001.1.
    BC058617 mRNA. Translation: AAH58617.1.
    CCDSiCCDS24896.1.
    RefSeqiNP_031937.1. NM_007911.5.
    UniGeneiMm.249637.

    Genome annotation databases

    EnsembliENSMUST00000004036; ENSMUSP00000004036; ENSMUSG00000003934.
    GeneIDi13643.
    KEGGimmu:13643.
    UCSCiuc007jqi.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF025288 mRNA. Translation: AAC53537.1 .
    BC052001 mRNA. Translation: AAH52001.1 .
    BC058617 mRNA. Translation: AAH58617.1 .
    CCDSi CCDS24896.1.
    RefSeqi NP_031937.1. NM_007911.5.
    UniGenei Mm.249637.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3D12 X-ray 3.00 B/E 29-169 [» ]
    ProteinModelPortali O35393.
    SMRi O35393. Positions 29-169.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-44833N.
    IntActi O35393. 3 interactions.
    MINTi MINT-1793420.
    STRINGi 10090.ENSMUSP00000004036.

    PTM databases

    PhosphoSitei O35393.

    Proteomic databases

    MaxQBi O35393.
    PaxDbi O35393.
    PRIDEi O35393.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000004036 ; ENSMUSP00000004036 ; ENSMUSG00000003934 .
    GeneIDi 13643.
    KEGGi mmu:13643.
    UCSCi uc007jqi.2. mouse.

    Organism-specific databases

    CTDi 1949.
    MGIi MGI:109196. Efnb3.

    Phylogenomic databases

    eggNOGi NOG268537.
    HOGENOMi HOG000220931.
    HOVERGENi HBG051448.
    InParanoidi O35393.
    KOi K05463.
    OMAi PGKENMP.
    OrthoDBi EOG7288S5.
    PhylomeDBi O35393.

    Miscellaneous databases

    EvolutionaryTracei O35393.
    NextBioi 284350.
    PROi O35393.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O35393.
    Bgeei O35393.
    CleanExi MM_EFNB3.
    Genevestigatori O35393.

    Family and domain databases

    Gene3Di 2.60.40.420. 1 hit.
    InterProi IPR008972. Cupredoxin.
    IPR001799. Ephrin.
    IPR019765. Ephrin_CS.
    [Graphical view ]
    PANTHERi PTHR11304. PTHR11304. 1 hit.
    Pfami PF00812. Ephrin. 1 hit.
    [Graphical view ]
    PRINTSi PR01347. EPHRIN.
    ProDomi PD002533. Ephrin. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF49503. SSF49503. 1 hit.
    PROSITEi PS01299. EPHRIN_RBD_1. 1 hit.
    PS51551. EPHRIN_RBD_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Ephrin-B3, a ligand for the receptor EphB3, expressed at the midline of the developing neural tube."
      Bergemann A.D., Zhang L., Chiang M.-K., Brambilla R., Klein R., Flanagan J.G.
      Oncogene 16:471-480(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    3. "Complementary expression of transmembrane ephrins and their receptors in the mouse spinal cord: a possible role in constraining the orientation of longitudinally projecting axons."
      Imondi R., Wideman C., Kaprielian Z.
      Development 127:1397-1410(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
      Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
      Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-210.

    Entry informationi

    Entry nameiEFNB3_MOUSE
    AccessioniPrimary (citable) accession number: O35393
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3