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O35393 (EFNB3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ephrin-B3
Gene names
Name:Efnb3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. May play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons. Ref.3

Subunit structure

Interacts with GRIP1 and GRIP2 By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Expressed on lateral floor plate cells, specifically on commissural axon segments that have passed through the floor plate. Expressed in cells of the retinal ganglion cell layer during retinal axon guidance to the optic disk.

Developmental stage

Expressed in the floor plate throughout the period of commissural axon pathfinding.

Sequence similarities

Belongs to the ephrin family.

Contains 1 ephrin RBD (ephrin receptor-binding) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 340313Ephrin-B3
PRO_0000008396

Regions

Topological domain28 – 227200Extracellular Potential
Transmembrane228 – 24821Helical; Potential
Topological domain249 – 34092Cytoplasmic Potential
Domain28 – 167140Ephrin RBD
Motif338 – 3403PDZ-binding Potential

Amino acid modifications

Glycosylation2101N-linked (GlcNAc...) Ref.4
Disulfide bond62 ↔ 104 By similarity
Disulfide bond92 ↔ 156 By similarity

Secondary structure

....................... 340
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O35393 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 52F3D58FD209A6B8

FASTA34035,885
        10         20         30         40         50         60 
MGAPHFGPGG VQVGALLLLG FAGLVSGLSL EPVYWNSANK RFQAEGGYVL YPQIGDRLDL 

        70         80         90        100        110        120 
LCPRARPPGP HSSPSYEFYK LYLVEGAQGR RCEAPPAPNL LLTCDRPDLD LRFTIKFQEY 

       130        140        150        160        170        180 
SPNLWGHEFR SHHDYYIIAT SDGTREGLES LQGGVCLTRG MKVLLRVGQS PRGGAVPRKP 

       190        200        210        220        230        240 
VSEMPMERDR GAAHSAEPGR DTIPGDPSSN ATSRGAEGPL PPPSMPAVAG AAGGMALLLL 

       250        260        270        280        290        300 
GVAGAGGAMC WRRRRAKPSE SRHPGPGSFG RGGSLGLGGG GGMGPREAEP GELGIALRGG 

       310        320        330        340 
GTADPPFCPH YEKVSGDYGH PVYIVQDGPP QSPPNIYYKV 

« Hide

References

« Hide 'large scale' references
[1]"Ephrin-B3, a ligand for the receptor EphB3, expressed at the midline of the developing neural tube."
Bergemann A.D., Zhang L., Chiang M.-K., Brambilla R., Klein R., Flanagan J.G.
Oncogene 16:471-480(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[3]"Complementary expression of transmembrane ephrins and their receptors in the mouse spinal cord: a possible role in constraining the orientation of longitudinally projecting axons."
Imondi R., Wideman C., Kaprielian Z.
Development 127:1397-1410(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-210.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF025288 mRNA. Translation: AAC53537.1.
BC052001 mRNA. Translation: AAH52001.1.
BC058617 mRNA. Translation: AAH58617.1.
RefSeqNP_031937.1. NM_007911.5.
UniGeneMm.249637.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3D12X-ray3.00B/E29-169[»]
ProteinModelPortalO35393.
SMRO35393. Positions 29-169.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-44833N.
IntActO35393. 3 interactions.
MINTMINT-1793420.
STRING10090.ENSMUSP00000004036.

PTM databases

PhosphoSiteO35393.

Proteomic databases

PaxDbO35393.
PRIDEO35393.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000004036; ENSMUSP00000004036; ENSMUSG00000003934.
GeneID13643.
KEGGmmu:13643.
UCSCuc007jqi.2. mouse.

Organism-specific databases

CTD1949.
MGIMGI:109196. Efnb3.

Phylogenomic databases

eggNOGNOG268537.
HOGENOMHOG000220931.
HOVERGENHBG051448.
InParanoidO35393.
KOK05463.
OMAPGKENMP.
OrthoDBEOG7288S5.
PhylomeDBO35393.

Gene expression databases

ArrayExpressO35393.
BgeeO35393.
CleanExMM_EFNB3.
GenevestigatorO35393.

Family and domain databases

Gene3D2.60.40.420. 1 hit.
InterProIPR008972. Cupredoxin.
IPR001799. Ephrin.
IPR019765. Ephrin_CS.
[Graphical view]
PANTHERPTHR11304. PTHR11304. 1 hit.
PfamPF00812. Ephrin. 1 hit.
[Graphical view]
PRINTSPR01347. EPHRIN.
ProDomPD002533. Ephrin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF49503. SSF49503. 1 hit.
PROSITEPS01299. EPHRIN_RBD_1. 1 hit.
PS51551. EPHRIN_RBD_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO35393.
NextBio284350.
PROO35393.
SOURCESearch...

Entry information

Entry nameEFNB3_MOUSE
AccessionPrimary (citable) accession number: O35393
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot