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Protein

HCLS1-associated protein X-1

Gene

Hax1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Recruits the Arp2/3 complex to the cell cortex and regulates reorganization of the cortical actin cytoskeleton via its interaction with KCNC3 and the Arp2/3 complex. Slows down the rate of inactivation of KCNC3 channels. Promotes GNA13-mediated cell migration. Involved in the clathrin-mediated endocytosis pathway. May be involved in internalization of ABC transporters such as ABCB11. May inhibit CASP9 and CASP3. Promotes cell survival. May regulate intracellular calcium pools.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
HCLS1-associated protein X-1
Alternative name(s):
HS1-associating protein X-1
Short name:
HAX-1
HS1-binding protein 1
Short name:
HSP1BP-1
Gene namesi
Name:Hax1
Synonyms:Hs1bp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1346319. Hax1.

Subcellular locationi

GO - Cellular componenti

  • actin cytoskeleton Source: BHF-UCL
  • cell cortex Source: UniProtKB-SubCell
  • cytoplasm Source: BHF-UCL
  • cytoplasmic, membrane-bounded vesicle Source: UniProtKB-SubCell
  • cytoplasmic mRNA processing body Source: UniProtKB-SubCell
  • endoplasmic reticulum Source: BHF-UCL
  • lamellipodium Source: BHF-UCL
  • mitochondrial intermembrane space Source: MGI
  • mitochondrial outer membrane Source: MGI
  • mitochondrion Source: MGI
  • nuclear membrane Source: UniProtKB-SubCell
  • plasma membrane Source: UniProtKB-SubCell
  • sarcoplasmic reticulum Source: CACAO
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane, Mitochondrion, Nucleus, Sarcoplasmic reticulum

Pathology & Biotechi

Disruption phenotypei

Mice lacking Hax1 fail to survive longer than 14 weeks, due to a loss of motor coordination and activity, leading to failure to eat and drink. They display extensive apoptosis of neurons in the striatum and cerebellum, and a loss of lymphocytes in spleen, bone marrow and thymus.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000839072 – 280HCLS1-associated protein X-1Add BLAST279

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei190PhosphoserineBy similarity1
Modified residuei193PhosphoserineBy similarity1

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei128 – 129Cleavage; by caspase-3By similarity2

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO35387.
MaxQBiO35387.
PaxDbiO35387.
PRIDEiO35387.

PTM databases

iPTMnetiO35387.
PhosphoSitePlusiO35387.

Expressioni

Tissue specificityi

Ubiquitous, with highest levels in kidney and liver (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000027944.
CleanExiMM_HAX1.
ExpressionAtlasiO35387. baseline and differential.
GenevisibleiO35387. MM.

Interactioni

Subunit structurei

Interacts with ABCB1, ABCB4 and ABCB11 (By similarity). Directly associates with HCLS1/HS1, through binding to its N-terminal region (By similarity). Interacts with CTTN (PubMed:10760273). Interacts with PKD2(PubMed:10760273). Interacts with GNA13. Interacts with CASP9. Interacts with ITGB6. Interacts with PLN and ATP2A2; these interactions are inhibited by calcium. Interacts with GRB7. Interacts (via C-terminus) with XIAP/BIRC4 (via BIR 2 domain and BIR 3 domain) and this interaction blocks ubiquitination of XIAP/BIRC4. Interacts with TPC2. Interacts with KCNC3 (PubMed:26997484). Interacts with XPO1 (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Arrb1Q8BWG86EBI-642449,EBI-641778
PKD2Q135633EBI-642449,EBI-7813714From a different organism.
Stat3P4222711EBI-642449,EBI-602878

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204783. 15 interactors.
DIPiDIP-49445N.
IntActiO35387. 19 interactors.
MINTiMINT-1592061.
STRINGi10090.ENSMUSP00000078661.

Structurei

3D structure databases

ProteinModelPortaliO35387.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 45Required for localization in mitochondriaBy similarityAdd BLAST44
Regioni115 – 280Involved in HCLS1 bindingBy similarityAdd BLAST166
Regioni176 – 207Involved in CASP9 bindingBy similarityAdd BLAST32
Regioni177 – 248Involved in GNA13 bindingBy similarityAdd BLAST72
Regioni184 – 280Required for localization in sarcoplasmic reticulumBy similarityAdd BLAST97
Regioni185 – 280Involved in PKD2 bindingAdd BLAST96
Regioni204 – 246Involved in ATP2A2 bindingBy similarityAdd BLAST43
Regioni204 – 226Involved in PLN bindingBy similarityAdd BLAST23
Regioni271 – 280Required for ITGB6 bindingBy similarity10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi30 – 44Asp/Glu-rich (highly acidic)Add BLAST15

Sequence similaritiesi

Belongs to the HAX1 family.Curated

Phylogenomic databases

eggNOGiENOG410IJQ0. Eukaryota.
ENOG411211U. LUCA.
GeneTreeiENSGT00390000018324.
HOGENOMiHOG000090247.
HOVERGENiHBG002991.
InParanoidiO35387.
KOiK16220.
OMAiPKPAPDW.
OrthoDBiEOG091G0MK9.
PhylomeDBiO35387.
TreeFamiTF328619.

Family and domain databases

InterProiIPR017248. HAX-1.
[Graphical view]
PIRSFiPIRSF037634. HS1-associating_X-1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35387-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVFDLFRGF FGFPGPRSHR DPFFGGMTRD DDDDDDDDDE AEEDRGAWGR
60 70 80 90 100
ESYAFDGSQP PEEFGFSFSP RGGMRFHGNF GFDDLVRDFN SIFSEMGAWT
110 120 130 140 150
LPSHSPELPG PESETPGERL REGQTLRDSM LKYPDSHQPR IFEGVLESHA
160 170 180 190 200
KPESPKPAPD WGSQGPFHRL DDTWPVSPHS RAKEDKDLDS QVSQEGLGPL
210 220 230 240 250
LQPQPKSYFK SISVTKITKP DGTVEERRTV VDSEGRRETT VTHQEAHDSS
260 270 280
RSDPDSQRSS ALDDPFSILD LLLGRWFRSR
Length:280
Mass (Da):31,654
Last modified:January 1, 1998 - v1
Checksum:i3123979BA10B45D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF023482 mRNA. Translation: AAB81081.1.
AK002256 mRNA. Translation: BAB21969.1.
AK010633 mRNA. Translation: BAB27077.1.
AK088746 mRNA. Translation: BAC40544.1.
BC006688 mRNA. Translation: AAH06688.1.
BC098225 mRNA. Translation: AAH98225.1.
CCDSiCCDS17519.1.
RefSeqiNP_035956.1. NM_011826.4.
UniGeneiMm.256035.
Mm.416677.

Genome annotation databases

EnsembliENSMUST00000079724; ENSMUSP00000078661; ENSMUSG00000027944.
GeneIDi23897.
KEGGimmu:23897.
UCSCiuc008qaj.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF023482 mRNA. Translation: AAB81081.1.
AK002256 mRNA. Translation: BAB21969.1.
AK010633 mRNA. Translation: BAB27077.1.
AK088746 mRNA. Translation: BAC40544.1.
BC006688 mRNA. Translation: AAH06688.1.
BC098225 mRNA. Translation: AAH98225.1.
CCDSiCCDS17519.1.
RefSeqiNP_035956.1. NM_011826.4.
UniGeneiMm.256035.
Mm.416677.

3D structure databases

ProteinModelPortaliO35387.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204783. 15 interactors.
DIPiDIP-49445N.
IntActiO35387. 19 interactors.
MINTiMINT-1592061.
STRINGi10090.ENSMUSP00000078661.

PTM databases

iPTMnetiO35387.
PhosphoSitePlusiO35387.

Proteomic databases

EPDiO35387.
MaxQBiO35387.
PaxDbiO35387.
PRIDEiO35387.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079724; ENSMUSP00000078661; ENSMUSG00000027944.
GeneIDi23897.
KEGGimmu:23897.
UCSCiuc008qaj.2. mouse.

Organism-specific databases

CTDi10456.
MGIiMGI:1346319. Hax1.

Phylogenomic databases

eggNOGiENOG410IJQ0. Eukaryota.
ENOG411211U. LUCA.
GeneTreeiENSGT00390000018324.
HOGENOMiHOG000090247.
HOVERGENiHBG002991.
InParanoidiO35387.
KOiK16220.
OMAiPKPAPDW.
OrthoDBiEOG091G0MK9.
PhylomeDBiO35387.
TreeFamiTF328619.

Miscellaneous databases

PROiO35387.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027944.
CleanExiMM_HAX1.
ExpressionAtlasiO35387. baseline and differential.
GenevisibleiO35387. MM.

Family and domain databases

InterProiIPR017248. HAX-1.
[Graphical view]
PIRSFiPIRSF037634. HS1-associating_X-1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHAX1_MOUSE
AccessioniPrimary (citable) accession number: O35387
Secondary accession number(s): Q542F8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.