Serine/threonine-protein phosphatase with EF-hands 2

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O35385 (PPE2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 122. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase with EF-hands 2
Short name=PPEF-2
EC=3.1.3.16

Gene names

Name:

Ppef2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	757 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May play a role in phototransduction. May dephosphorylate photoactivated rhodopsin. May function as a calcium sensing regulator of ionic currents, energy production or synaptic transmission.
Catalytic activity	A phosphoprotein + H₂O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Enzyme regulation	Activated by calcium By similarity.
Tissue specificity	Detected in retina, more specifically in photoreceptors.
Sequence similarities	Belongs to the PPP phosphatase family. Contains 3 EF-hand domains. Contains 1 IQ domain.

Ontologies

Keywords
Biological process	Sensory transduction Vision
Domain	Repeat
Ligand	Calcium Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	detection of stimulus involved in sensory perception Inferred from electronic annotation. Source: InterPro negative regulation of MAPK cascade Inferred from electronic annotation. Source: Compara negative regulation of peptidyl-threonine phosphorylation Inferred from electronic annotation. Source: Compara visual perception Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro manganese ion binding Inferred from electronic annotation. Source: InterPro protein serine/threonine phosphatase activity Inferred from sequence or structural similarity Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 757

757

Serine/threonine-protein phosphatase with EF-hands 2

PRO_0000058902

Regions

Domain

21 – 46

Domain

572 – 607

EF-hand 1

Domain

656 – 691

EF-hand 2

Domain

696 – 731

EF-hand 3

Calcium binding

669 – 680

1 Potential

Calcium binding

709 – 720

2 Potential

Region

128 – 544

417

Catalytic

Sites

Active site

241

Proton donor By similarity

Metal binding

179

Iron By similarity

Metal binding

181

Iron By similarity

Metal binding

208

Iron By similarity

Metal binding

208

Manganese By similarity

Metal binding

240

Manganese By similarity

Metal binding

292

Manganese By similarity

Metal binding

492

Manganese By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

O35385 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 821B3D1061AC00C8
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FASTA

757

86,645

        10         20         30         40         50         60 
MGSSSSTQHH FAFQNAEKAF KAAALIQRWY RRYMARLEMR RRCTWNIFQS IEYAGQQDQV 

        70         80         90        100        110        120 
KLHEFFSYLV DHFTPSSHHE RDFLNRMFTE ERFAQDVETE EGGDFESIEV PDSYTGPRLS 

       130        140        150        160        170        180 
FPLLPDHATA LVEAFRLRQQ LHARYVLNLL YETRKHLAQL PNINRVSTCY SEEVTVCGDL 

       190        200        210        220        230        240 
HGQLDDLIFI FYKNGLPSPE RAYVFNGDFV DRGKDSVEVL MVLFAFMLVY PKEFHLNRGN 

       250        260        270        280        290        300 
HEDHLVNLRY GFTKEVMHKY KIHGKKILRT LQDVFCWLPL ATLVDEKVLV LHGGVSDKTD 

       310        320        330        340        350        360 
LELLAKLDRH KIVSTMRCKT RKESENREEQ KRKDNQTSSG QKPTPWFLPQ SRSLPSSPFH 

       370        380        390        400        410        420 
LGSGFKAYKA GRSCSIPCGS PNSKELSRRG QVRRSVDLEL EQCRQQAGFL GIREKGESLP 

       430        440        450        460        470        480 
LAPDADCVAD GGGVLEPTPE EWKQVVDILW SDPAAQEGCK ANAVRGGGCY FGPDVTERLM 

       490        500        510        520        530        540 
EKYKLQLLIR SHECKPEGYE FCHNRKVLTI FSASNYYEVG SNRGAYVKLG PALTPHIVQY 

       550        560        570        580        590        600 
QANKATHRLT MRQRISRVEE SALRALRQKL FAHSSDLLVE FRKRDPDESG VITLSDWATA 

       610        620        630        640        650        660 
VESVLHLGLP WRMLRPQLVN SSADNVLEYR SWLDSLAKEQ LSRENIQSSL LEKLYRNRSN 

       670        680        690        700        710        720 
LETIFRIIDS DHSGFISLDE FRQTWKLFSS HMSIDITDDG ICDLARSIDF NKDGHIDINE 

       730        740        750 
FLEAFRLVEQ SCLEGHASAC LQSTDTAESG HSSPGPC

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a conserved family of protein serine/threonine phosphatases homologous to Drosophila retinal degeneration C." Sherman P.M., Sun H., Macke J.P., Williams J., Smallwood P.M., Nathans J. Proc. Natl. Acad. Sci. U.S.A. 94:11639-11644(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Eye.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Retina.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF023458 mRNA. Translation: AAB82798.1. BC027049 mRNA. Translation: AAH27049.1.
IPI	IPI00129962.
RefSeq	NP_035278.1. NM_011148.3.
UniGene	Mm.87155.
3D structure databases
ProteinModelPortal	O35385.
SMR	O35385. Positions 131-311, 439-542, 574-603, 609-729.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000031359.
PTM databases
PhosphoSite	O35385.
Proteomic databases
PaxDb	O35385.
PRIDE	O35385.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000031359; ENSMUSP00000031359; ENSMUSG00000029410.
GeneID	19023.
KEGG	mmu:19023.
UCSC	uc008yco.2. mouse.
Organism-specific databases
CTD	5470.
MGI	MGI:1342304. Ppef2.
Phylogenomic databases
eggNOG	COG0639.
GeneTree	ENSGT00530000063173.
HOGENOM	HOG000006820.
HOVERGEN	HBG008238.
InParanoid	O35385.
KO	K01090.
OMA	NMLEYKS.
OrthoDB	EOG4255SB.
Gene expression databases
Bgee	O35385.
Genevestigator	O35385.
GermOnline	ENSMUSG00000029410. Mus musculus.
Family and domain databases
Gene3D	1.10.238.10. 2 hits.
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. IPR000048. IQ_motif_EF-hand-BS. IPR004843. Metallo_PEstase_dom. IPR013235. PPP_dom. IPR012008. Ser/Thr-Pase_EF-hand_contain. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF13499. EF_hand_5. 1 hit. PF00149. Metallophos. 1 hit. PF08321. PPP5. 1 hit. [Graphical view]
PIRSF	PIRSF000912. PPEF. 1 hit.
PRINTS	PR00114. STPHPHTASE.
SMART	SM00054. EFh. 3 hits. SM00015. IQ. 1 hit. SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 3 hits. PS50222. EF_HAND_2. 3 hits. PS50096. IQ. 1 hit. PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	295456.
SOURCE	Search...

Entry information

Entry name

PPE2_MOUSE

Accession

Primary (citable) accession number: O35385

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 122 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents