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Protein

Serine/threonine-protein phosphatase with EF-hands 2

Gene

Ppef2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May play a role in phototransduction. May dephosphorylate photoactivated rhodopsin. May function as a calcium sensing regulator of ionic currents, energy production or synaptic transmission.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Enzyme regulationi

Activated by calcium.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi179 – 1791Manganese 1By similarity
Metal bindingi181 – 1811Manganese 1By similarity
Metal bindingi208 – 2081Manganese 1By similarity
Metal bindingi208 – 2081Manganese 2By similarity
Metal bindingi240 – 2401Manganese 2By similarity
Active sitei241 – 2411Proton donorBy similarity
Metal bindingi292 – 2921Manganese 2By similarity
Metal bindingi492 – 4921Manganese 2By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi669 – 680121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi709 – 720122PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. Hsp70 protein binding Source: MGI
  3. Hsp90 protein binding Source: MGI
  4. iron ion binding Source: InterPro
  5. manganese ion binding Source: InterPro
  6. mitogen-activated protein kinase kinase kinase binding Source: MGI
  7. protein serine/threonine phosphatase activity Source: MGI

GO - Biological processi

  1. dephosphorylation Source: GOC
  2. detection of stimulus involved in sensory perception Source: InterPro
  3. negative regulation of MAPK cascade Source: MGI
  4. negative regulation of peptidyl-threonine phosphorylation Source: MGI
  5. visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

Calcium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase with EF-hands 2 (EC:3.1.3.16)
Short name:
PPEF-2
Gene namesi
Name:Ppef2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:1342304. Ppef2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 757757Serine/threonine-protein phosphatase with EF-hands 2PRO_0000058902Add
BLAST

Proteomic databases

PaxDbiO35385.
PRIDEiO35385.

PTM databases

PhosphoSiteiO35385.

Expressioni

Tissue specificityi

Detected in retina, more specifically in photoreceptors.

Gene expression databases

BgeeiO35385.
GenevestigatoriO35385.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031359.

Structurei

3D structure databases

ProteinModelPortaliO35385.
SMRiO35385. Positions 127-550, 577-603, 609-728.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 4626IQPROSITE-ProRule annotationAdd
BLAST
Domaini572 – 60736EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini656 – 69136EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini696 – 73136EF-hand 3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni128 – 544417CatalyticAdd
BLAST

Sequence similaritiesi

Belongs to the PPP phosphatase family.Curated
Contains 3 EF-hand domains.PROSITE-ProRule annotation
Contains 1 IQ domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00530000063173.
HOGENOMiHOG000006820.
HOVERGENiHBG008238.
InParanoidiO35385.
KOiK13807.
OMAiKLHNFFS.
OrthoDBiEOG7KH9J7.
PhylomeDBiO35385.
TreeFamiTF313342.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
3.60.21.10. 2 hits.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000048. IQ_motif_EF-hand-BS.
IPR029052. Metallo-depent_PP-like.
IPR013235. PPP_dom.
IPR012008. Ser/Thr-Pase_EF-hand_contain.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF13499. EF-hand_7. 1 hit.
PF00149. Metallophos. 1 hit.
PF08321. PPP5. 1 hit.
[Graphical view]
PIRSFiPIRSF000912. PPEF. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00054. EFh. 3 hits.
SM00015. IQ. 1 hit.
SM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 2 hits.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
PS50096. IQ. 1 hit.
PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35385-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGSSSSTQHH FAFQNAEKAF KAAALIQRWY RRYMARLEMR RRCTWNIFQS
60 70 80 90 100
IEYAGQQDQV KLHEFFSYLV DHFTPSSHHE RDFLNRMFTE ERFAQDVETE
110 120 130 140 150
EGGDFESIEV PDSYTGPRLS FPLLPDHATA LVEAFRLRQQ LHARYVLNLL
160 170 180 190 200
YETRKHLAQL PNINRVSTCY SEEVTVCGDL HGQLDDLIFI FYKNGLPSPE
210 220 230 240 250
RAYVFNGDFV DRGKDSVEVL MVLFAFMLVY PKEFHLNRGN HEDHLVNLRY
260 270 280 290 300
GFTKEVMHKY KIHGKKILRT LQDVFCWLPL ATLVDEKVLV LHGGVSDKTD
310 320 330 340 350
LELLAKLDRH KIVSTMRCKT RKESENREEQ KRKDNQTSSG QKPTPWFLPQ
360 370 380 390 400
SRSLPSSPFH LGSGFKAYKA GRSCSIPCGS PNSKELSRRG QVRRSVDLEL
410 420 430 440 450
EQCRQQAGFL GIREKGESLP LAPDADCVAD GGGVLEPTPE EWKQVVDILW
460 470 480 490 500
SDPAAQEGCK ANAVRGGGCY FGPDVTERLM EKYKLQLLIR SHECKPEGYE
510 520 530 540 550
FCHNRKVLTI FSASNYYEVG SNRGAYVKLG PALTPHIVQY QANKATHRLT
560 570 580 590 600
MRQRISRVEE SALRALRQKL FAHSSDLLVE FRKRDPDESG VITLSDWATA
610 620 630 640 650
VESVLHLGLP WRMLRPQLVN SSADNVLEYR SWLDSLAKEQ LSRENIQSSL
660 670 680 690 700
LEKLYRNRSN LETIFRIIDS DHSGFISLDE FRQTWKLFSS HMSIDITDDG
710 720 730 740 750
ICDLARSIDF NKDGHIDINE FLEAFRLVEQ SCLEGHASAC LQSTDTAESG

HSSPGPC
Length:757
Mass (Da):86,645
Last modified:January 1, 1998 - v1
Checksum:i821B3D1061AC00C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF023458 mRNA. Translation: AAB82798.1.
BC027049 mRNA. Translation: AAH27049.1.
CCDSiCCDS39151.1.
RefSeqiNP_035278.1. NM_011148.3.
XP_006534880.1. XM_006534817.1.
UniGeneiMm.87155.

Genome annotation databases

EnsembliENSMUST00000031359; ENSMUSP00000031359; ENSMUSG00000029410.
GeneIDi19023.
KEGGimmu:19023.
UCSCiuc008yco.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF023458 mRNA. Translation: AAB82798.1.
BC027049 mRNA. Translation: AAH27049.1.
CCDSiCCDS39151.1.
RefSeqiNP_035278.1. NM_011148.3.
XP_006534880.1. XM_006534817.1.
UniGeneiMm.87155.

3D structure databases

ProteinModelPortaliO35385.
SMRiO35385. Positions 127-550, 577-603, 609-728.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031359.

PTM databases

PhosphoSiteiO35385.

Proteomic databases

PaxDbiO35385.
PRIDEiO35385.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031359; ENSMUSP00000031359; ENSMUSG00000029410.
GeneIDi19023.
KEGGimmu:19023.
UCSCiuc008yco.2. mouse.

Organism-specific databases

CTDi5470.
MGIiMGI:1342304. Ppef2.

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00530000063173.
HOGENOMiHOG000006820.
HOVERGENiHBG008238.
InParanoidiO35385.
KOiK13807.
OMAiKLHNFFS.
OrthoDBiEOG7KH9J7.
PhylomeDBiO35385.
TreeFamiTF313342.

Miscellaneous databases

NextBioi295456.
PROiO35385.
SOURCEiSearch...

Gene expression databases

BgeeiO35385.
GenevestigatoriO35385.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
3.60.21.10. 2 hits.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000048. IQ_motif_EF-hand-BS.
IPR029052. Metallo-depent_PP-like.
IPR013235. PPP_dom.
IPR012008. Ser/Thr-Pase_EF-hand_contain.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF13499. EF-hand_7. 1 hit.
PF00149. Metallophos. 1 hit.
PF08321. PPP5. 1 hit.
[Graphical view]
PIRSFiPIRSF000912. PPEF. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00054. EFh. 3 hits.
SM00015. IQ. 1 hit.
SM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 2 hits.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
PS50096. IQ. 1 hit.
PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of a conserved family of protein serine/threonine phosphatases homologous to Drosophila retinal degeneration C."
    Sherman P.M., Sun H., Macke J.P., Williams J., Smallwood P.M., Nathans J.
    Proc. Natl. Acad. Sci. U.S.A. 94:11639-11644(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Eye.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Retina.

Entry informationi

Entry nameiPPE2_MOUSE
AccessioniPrimary (citable) accession number: O35385
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 1, 1998
Last modified: February 4, 2015
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.