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O35385 (PPE2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase with EF-hands 2

Short name=PPEF-2
EC=3.1.3.16
Gene names
Name:Ppef2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length757 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May play a role in phototransduction. May dephosphorylate photoactivated rhodopsin. May function as a calcium sensing regulator of ionic currents, energy production or synaptic transmission.

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 1 iron ion per subunit By similarity.

Binds 1 manganese ion per subunit By similarity.

Enzyme regulation

Activated by calcium By similarity.

Tissue specificity

Detected in retina, more specifically in photoreceptors.

Sequence similarities

Belongs to the PPP phosphatase family.

Contains 3 EF-hand domains.

Contains 1 IQ domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 757757Serine/threonine-protein phosphatase with EF-hands 2
PRO_0000058902

Regions

Domain21 – 4626IQ
Domain572 – 60736EF-hand 1
Domain656 – 69136EF-hand 2
Domain696 – 73136EF-hand 3
Calcium binding669 – 680121 Potential
Calcium binding709 – 720122 Potential
Region128 – 544417Catalytic

Sites

Active site2411Proton donor By similarity
Metal binding1791Iron By similarity
Metal binding1811Iron By similarity
Metal binding2081Iron By similarity
Metal binding2081Manganese By similarity
Metal binding2401Manganese By similarity
Metal binding2921Manganese By similarity
Metal binding4921Manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
O35385 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 821B3D1061AC00C8

FASTA75786,645
        10         20         30         40         50         60 
MGSSSSTQHH FAFQNAEKAF KAAALIQRWY RRYMARLEMR RRCTWNIFQS IEYAGQQDQV 

        70         80         90        100        110        120 
KLHEFFSYLV DHFTPSSHHE RDFLNRMFTE ERFAQDVETE EGGDFESIEV PDSYTGPRLS 

       130        140        150        160        170        180 
FPLLPDHATA LVEAFRLRQQ LHARYVLNLL YETRKHLAQL PNINRVSTCY SEEVTVCGDL 

       190        200        210        220        230        240 
HGQLDDLIFI FYKNGLPSPE RAYVFNGDFV DRGKDSVEVL MVLFAFMLVY PKEFHLNRGN 

       250        260        270        280        290        300 
HEDHLVNLRY GFTKEVMHKY KIHGKKILRT LQDVFCWLPL ATLVDEKVLV LHGGVSDKTD 

       310        320        330        340        350        360 
LELLAKLDRH KIVSTMRCKT RKESENREEQ KRKDNQTSSG QKPTPWFLPQ SRSLPSSPFH 

       370        380        390        400        410        420 
LGSGFKAYKA GRSCSIPCGS PNSKELSRRG QVRRSVDLEL EQCRQQAGFL GIREKGESLP 

       430        440        450        460        470        480 
LAPDADCVAD GGGVLEPTPE EWKQVVDILW SDPAAQEGCK ANAVRGGGCY FGPDVTERLM 

       490        500        510        520        530        540 
EKYKLQLLIR SHECKPEGYE FCHNRKVLTI FSASNYYEVG SNRGAYVKLG PALTPHIVQY 

       550        560        570        580        590        600 
QANKATHRLT MRQRISRVEE SALRALRQKL FAHSSDLLVE FRKRDPDESG VITLSDWATA 

       610        620        630        640        650        660 
VESVLHLGLP WRMLRPQLVN SSADNVLEYR SWLDSLAKEQ LSRENIQSSL LEKLYRNRSN 

       670        680        690        700        710        720 
LETIFRIIDS DHSGFISLDE FRQTWKLFSS HMSIDITDDG ICDLARSIDF NKDGHIDINE 

       730        740        750 
FLEAFRLVEQ SCLEGHASAC LQSTDTAESG HSSPGPC 

« Hide

References

« Hide 'large scale' references
[1]"Identification and characterization of a conserved family of protein serine/threonine phosphatases homologous to Drosophila retinal degeneration C."
Sherman P.M., Sun H., Macke J.P., Williams J., Smallwood P.M., Nathans J.
Proc. Natl. Acad. Sci. U.S.A. 94:11639-11644(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Eye.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Retina.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF023458 mRNA. Translation: AAB82798.1.
BC027049 mRNA. Translation: AAH27049.1.
RefSeqNP_035278.1. NM_011148.3.
XP_006534880.1. XM_006534817.1.
UniGeneMm.87155.

3D structure databases

ProteinModelPortalO35385.
SMRO35385. Positions 7-552, 576-728.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000031359.

PTM databases

PhosphoSiteO35385.

Proteomic databases

PaxDbO35385.
PRIDEO35385.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000031359; ENSMUSP00000031359; ENSMUSG00000029410.
GeneID19023.
KEGGmmu:19023.
UCSCuc008yco.2. mouse.

Organism-specific databases

CTD5470.
MGIMGI:1342304. Ppef2.

Phylogenomic databases

eggNOGCOG0639.
GeneTreeENSGT00530000063173.
HOGENOMHOG000006820.
HOVERGENHBG008238.
InParanoidO35385.
KOK13807.
OMANMLEYKS.
OrthoDBEOG7KH9J7.
PhylomeDBO35385.
TreeFamTF313342.

Gene expression databases

BgeeO35385.
GenevestigatorO35385.

Family and domain databases

Gene3D1.10.238.10. 2 hits.
InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000048. IQ_motif_EF-hand-BS.
IPR013235. PPP_dom.
IPR012008. Ser/Thr-Pase_EF-hand_contain.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF13499. EF-hand_7. 1 hit.
PF00149. Metallophos. 1 hit.
PF08321. PPP5. 1 hit.
[Graphical view]
PIRSFPIRSF000912. PPEF. 1 hit.
PRINTSPR00114. STPHPHTASE.
SMARTSM00054. EFh. 3 hits.
SM00015. IQ. 1 hit.
SM00156. PP2Ac. 1 hit.
[Graphical view]
PROSITEPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
PS50096. IQ. 1 hit.
PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio295456.
PROO35385.
SOURCESearch...

Entry information

Entry namePPE2_MOUSE
AccessionPrimary (citable) accession number: O35385
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot