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O35385

- PPE2_MOUSE

UniProt

O35385 - PPE2_MOUSE

Protein

Serine/threonine-protein phosphatase with EF-hands 2

Gene

Ppef2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    May play a role in phototransduction. May dephosphorylate photoactivated rhodopsin. May function as a calcium sensing regulator of ionic currents, energy production or synaptic transmission.

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Enzyme regulationi

    Activated by calcium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi179 – 1791Manganese 1By similarity
    Metal bindingi181 – 1811Manganese 1By similarity
    Metal bindingi208 – 2081Manganese 1By similarity
    Metal bindingi208 – 2081Manganese 2By similarity
    Metal bindingi240 – 2401Manganese 2By similarity
    Active sitei241 – 2411Proton donorBy similarity
    Metal bindingi292 – 2921Manganese 2By similarity
    Metal bindingi492 – 4921Manganese 2By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi669 – 680121PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi709 – 720122PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. iron ion binding Source: InterPro
    3. manganese ion binding Source: InterPro
    4. protein serine/threonine phosphatase activity Source: MGI

    GO - Biological processi

    1. dephosphorylation Source: GOC
    2. detection of stimulus involved in sensory perception Source: InterPro
    3. negative regulation of MAPK cascade Source: Ensembl
    4. negative regulation of peptidyl-threonine phosphorylation Source: Ensembl
    5. visual perception Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Sensory transduction, Vision

    Keywords - Ligandi

    Calcium, Manganese, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase with EF-hands 2 (EC:3.1.3.16)
    Short name:
    PPEF-2
    Gene namesi
    Name:Ppef2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1342304. Ppef2.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 757757Serine/threonine-protein phosphatase with EF-hands 2PRO_0000058902Add
    BLAST

    Proteomic databases

    PaxDbiO35385.
    PRIDEiO35385.

    PTM databases

    PhosphoSiteiO35385.

    Expressioni

    Tissue specificityi

    Detected in retina, more specifically in photoreceptors.

    Gene expression databases

    BgeeiO35385.
    GenevestigatoriO35385.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000031359.

    Structurei

    3D structure databases

    ProteinModelPortaliO35385.
    SMRiO35385. Positions 127-550, 556-728.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini21 – 4626IQPROSITE-ProRule annotationAdd
    BLAST
    Domaini572 – 60736EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini656 – 69136EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini696 – 73136EF-hand 3PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni128 – 544417CatalyticAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PPP phosphatase family.Curated
    Contains 3 EF-hand domains.PROSITE-ProRule annotation
    Contains 1 IQ domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0639.
    GeneTreeiENSGT00530000063173.
    HOGENOMiHOG000006820.
    HOVERGENiHBG008238.
    InParanoidiO35385.
    KOiK13807.
    OMAiNMLEYKS.
    OrthoDBiEOG7KH9J7.
    PhylomeDBiO35385.
    TreeFamiTF313342.

    Family and domain databases

    Gene3Di1.10.238.10. 2 hits.
    3.60.21.10. 2 hits.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000048. IQ_motif_EF-hand-BS.
    IPR029052. Metallo-depent_PP-like.
    IPR013235. PPP_dom.
    IPR012008. Ser/Thr-Pase_EF-hand_contain.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF13499. EF-hand_7. 1 hit.
    PF00149. Metallophos. 1 hit.
    PF08321. PPP5. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000912. PPEF. 1 hit.
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00054. EFh. 3 hits.
    SM00015. IQ. 1 hit.
    SM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 2 hits.
    PROSITEiPS00018. EF_HAND_1. 3 hits.
    PS50222. EF_HAND_2. 3 hits.
    PS50096. IQ. 1 hit.
    PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O35385-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSSSSTQHH FAFQNAEKAF KAAALIQRWY RRYMARLEMR RRCTWNIFQS    50
    IEYAGQQDQV KLHEFFSYLV DHFTPSSHHE RDFLNRMFTE ERFAQDVETE 100
    EGGDFESIEV PDSYTGPRLS FPLLPDHATA LVEAFRLRQQ LHARYVLNLL 150
    YETRKHLAQL PNINRVSTCY SEEVTVCGDL HGQLDDLIFI FYKNGLPSPE 200
    RAYVFNGDFV DRGKDSVEVL MVLFAFMLVY PKEFHLNRGN HEDHLVNLRY 250
    GFTKEVMHKY KIHGKKILRT LQDVFCWLPL ATLVDEKVLV LHGGVSDKTD 300
    LELLAKLDRH KIVSTMRCKT RKESENREEQ KRKDNQTSSG QKPTPWFLPQ 350
    SRSLPSSPFH LGSGFKAYKA GRSCSIPCGS PNSKELSRRG QVRRSVDLEL 400
    EQCRQQAGFL GIREKGESLP LAPDADCVAD GGGVLEPTPE EWKQVVDILW 450
    SDPAAQEGCK ANAVRGGGCY FGPDVTERLM EKYKLQLLIR SHECKPEGYE 500
    FCHNRKVLTI FSASNYYEVG SNRGAYVKLG PALTPHIVQY QANKATHRLT 550
    MRQRISRVEE SALRALRQKL FAHSSDLLVE FRKRDPDESG VITLSDWATA 600
    VESVLHLGLP WRMLRPQLVN SSADNVLEYR SWLDSLAKEQ LSRENIQSSL 650
    LEKLYRNRSN LETIFRIIDS DHSGFISLDE FRQTWKLFSS HMSIDITDDG 700
    ICDLARSIDF NKDGHIDINE FLEAFRLVEQ SCLEGHASAC LQSTDTAESG 750
    HSSPGPC 757
    Length:757
    Mass (Da):86,645
    Last modified:January 1, 1998 - v1
    Checksum:i821B3D1061AC00C8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF023458 mRNA. Translation: AAB82798.1.
    BC027049 mRNA. Translation: AAH27049.1.
    CCDSiCCDS39151.1.
    RefSeqiNP_035278.1. NM_011148.3.
    XP_006534880.1. XM_006534817.1.
    UniGeneiMm.87155.

    Genome annotation databases

    EnsembliENSMUST00000031359; ENSMUSP00000031359; ENSMUSG00000029410.
    GeneIDi19023.
    KEGGimmu:19023.
    UCSCiuc008yco.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF023458 mRNA. Translation: AAB82798.1 .
    BC027049 mRNA. Translation: AAH27049.1 .
    CCDSi CCDS39151.1.
    RefSeqi NP_035278.1. NM_011148.3.
    XP_006534880.1. XM_006534817.1.
    UniGenei Mm.87155.

    3D structure databases

    ProteinModelPortali O35385.
    SMRi O35385. Positions 127-550, 556-728.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000031359.

    PTM databases

    PhosphoSitei O35385.

    Proteomic databases

    PaxDbi O35385.
    PRIDEi O35385.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000031359 ; ENSMUSP00000031359 ; ENSMUSG00000029410 .
    GeneIDi 19023.
    KEGGi mmu:19023.
    UCSCi uc008yco.2. mouse.

    Organism-specific databases

    CTDi 5470.
    MGIi MGI:1342304. Ppef2.

    Phylogenomic databases

    eggNOGi COG0639.
    GeneTreei ENSGT00530000063173.
    HOGENOMi HOG000006820.
    HOVERGENi HBG008238.
    InParanoidi O35385.
    KOi K13807.
    OMAi NMLEYKS.
    OrthoDBi EOG7KH9J7.
    PhylomeDBi O35385.
    TreeFami TF313342.

    Miscellaneous databases

    NextBioi 295456.
    PROi O35385.
    SOURCEi Search...

    Gene expression databases

    Bgeei O35385.
    Genevestigatori O35385.

    Family and domain databases

    Gene3Di 1.10.238.10. 2 hits.
    3.60.21.10. 2 hits.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000048. IQ_motif_EF-hand-BS.
    IPR029052. Metallo-depent_PP-like.
    IPR013235. PPP_dom.
    IPR012008. Ser/Thr-Pase_EF-hand_contain.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF13499. EF-hand_7. 1 hit.
    PF00149. Metallophos. 1 hit.
    PF08321. PPP5. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000912. PPEF. 1 hit.
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00054. EFh. 3 hits.
    SM00015. IQ. 1 hit.
    SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 2 hits.
    PROSITEi PS00018. EF_HAND_1. 3 hits.
    PS50222. EF_HAND_2. 3 hits.
    PS50096. IQ. 1 hit.
    PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of a conserved family of protein serine/threonine phosphatases homologous to Drosophila retinal degeneration C."
      Sherman P.M., Sun H., Macke J.P., Williams J., Smallwood P.M., Nathans J.
      Proc. Natl. Acad. Sci. U.S.A. 94:11639-11644(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Eye.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Retina.

    Entry informationi

    Entry nameiPPE2_MOUSE
    AccessioniPrimary (citable) accession number: O35385
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 134 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3