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O35385

- PPE2_MOUSE

UniProt

O35385 - PPE2_MOUSE

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Protein

Serine/threonine-protein phosphatase with EF-hands 2

Gene
Ppef2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

May play a role in phototransduction. May dephosphorylate photoactivated rhodopsin. May function as a calcium sensing regulator of ionic currents, energy production or synaptic transmission.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 manganese ions per subunit By similarity.

Enzyme regulationi

Activated by calcium By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi179 – 1791Manganese 1 By similarity
Metal bindingi181 – 1811Manganese 1 By similarity
Metal bindingi208 – 2081Manganese 1 By similarity
Metal bindingi208 – 2081Manganese 2 By similarity
Metal bindingi240 – 2401Manganese 2 By similarity
Active sitei241 – 2411Proton donor By similarity
Metal bindingi292 – 2921Manganese 2 By similarity
Metal bindingi492 – 4921Manganese 2 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi669 – 680121 Reviewed predictionAdd
BLAST
Calcium bindingi709 – 720122 Reviewed predictionAdd
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. iron ion binding Source: InterPro
  3. manganese ion binding Source: InterPro
  4. protein serine/threonine phosphatase activity Source: MGI

GO - Biological processi

  1. dephosphorylation Source: GOC
  2. detection of stimulus involved in sensory perception Source: InterPro
  3. negative regulation of MAPK cascade Source: Ensembl
  4. negative regulation of peptidyl-threonine phosphorylation Source: Ensembl
  5. visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

Calcium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase with EF-hands 2 (EC:3.1.3.16)
Short name:
PPEF-2
Gene namesi
Name:Ppef2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:1342304. Ppef2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 757757Serine/threonine-protein phosphatase with EF-hands 2PRO_0000058902Add
BLAST

Proteomic databases

PaxDbiO35385.
PRIDEiO35385.

PTM databases

PhosphoSiteiO35385.

Expressioni

Tissue specificityi

Detected in retina, more specifically in photoreceptors.

Gene expression databases

BgeeiO35385.
GenevestigatoriO35385.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031359.

Structurei

3D structure databases

ProteinModelPortaliO35385.
SMRiO35385. Positions 127-550, 556-728.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 4626IQAdd
BLAST
Domaini572 – 60736EF-hand 1Add
BLAST
Domaini656 – 69136EF-hand 2Add
BLAST
Domaini696 – 73136EF-hand 3Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni128 – 544417CatalyticAdd
BLAST

Sequence similaritiesi

Belongs to the PPP phosphatase family.
Contains 3 EF-hand domains.
Contains 1 IQ domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00530000063173.
HOGENOMiHOG000006820.
HOVERGENiHBG008238.
InParanoidiO35385.
KOiK13807.
OMAiNMLEYKS.
OrthoDBiEOG7KH9J7.
PhylomeDBiO35385.
TreeFamiTF313342.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
3.60.21.10. 2 hits.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000048. IQ_motif_EF-hand-BS.
IPR029052. Metallo-depent_PP-like.
IPR013235. PPP_dom.
IPR012008. Ser/Thr-Pase_EF-hand_contain.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF13499. EF-hand_7. 1 hit.
PF00149. Metallophos. 1 hit.
PF08321. PPP5. 1 hit.
[Graphical view]
PIRSFiPIRSF000912. PPEF. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00054. EFh. 3 hits.
SM00015. IQ. 1 hit.
SM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 2 hits.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
PS50096. IQ. 1 hit.
PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35385-1 [UniParc]FASTAAdd to Basket

« Hide

MGSSSSTQHH FAFQNAEKAF KAAALIQRWY RRYMARLEMR RRCTWNIFQS    50
IEYAGQQDQV KLHEFFSYLV DHFTPSSHHE RDFLNRMFTE ERFAQDVETE 100
EGGDFESIEV PDSYTGPRLS FPLLPDHATA LVEAFRLRQQ LHARYVLNLL 150
YETRKHLAQL PNINRVSTCY SEEVTVCGDL HGQLDDLIFI FYKNGLPSPE 200
RAYVFNGDFV DRGKDSVEVL MVLFAFMLVY PKEFHLNRGN HEDHLVNLRY 250
GFTKEVMHKY KIHGKKILRT LQDVFCWLPL ATLVDEKVLV LHGGVSDKTD 300
LELLAKLDRH KIVSTMRCKT RKESENREEQ KRKDNQTSSG QKPTPWFLPQ 350
SRSLPSSPFH LGSGFKAYKA GRSCSIPCGS PNSKELSRRG QVRRSVDLEL 400
EQCRQQAGFL GIREKGESLP LAPDADCVAD GGGVLEPTPE EWKQVVDILW 450
SDPAAQEGCK ANAVRGGGCY FGPDVTERLM EKYKLQLLIR SHECKPEGYE 500
FCHNRKVLTI FSASNYYEVG SNRGAYVKLG PALTPHIVQY QANKATHRLT 550
MRQRISRVEE SALRALRQKL FAHSSDLLVE FRKRDPDESG VITLSDWATA 600
VESVLHLGLP WRMLRPQLVN SSADNVLEYR SWLDSLAKEQ LSRENIQSSL 650
LEKLYRNRSN LETIFRIIDS DHSGFISLDE FRQTWKLFSS HMSIDITDDG 700
ICDLARSIDF NKDGHIDINE FLEAFRLVEQ SCLEGHASAC LQSTDTAESG 750
HSSPGPC 757
Length:757
Mass (Da):86,645
Last modified:January 1, 1998 - v1
Checksum:i821B3D1061AC00C8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF023458 mRNA. Translation: AAB82798.1.
BC027049 mRNA. Translation: AAH27049.1.
CCDSiCCDS39151.1.
RefSeqiNP_035278.1. NM_011148.3.
XP_006534880.1. XM_006534817.1.
UniGeneiMm.87155.

Genome annotation databases

EnsembliENSMUST00000031359; ENSMUSP00000031359; ENSMUSG00000029410.
GeneIDi19023.
KEGGimmu:19023.
UCSCiuc008yco.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF023458 mRNA. Translation: AAB82798.1 .
BC027049 mRNA. Translation: AAH27049.1 .
CCDSi CCDS39151.1.
RefSeqi NP_035278.1. NM_011148.3.
XP_006534880.1. XM_006534817.1.
UniGenei Mm.87155.

3D structure databases

ProteinModelPortali O35385.
SMRi O35385. Positions 127-550, 556-728.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000031359.

PTM databases

PhosphoSitei O35385.

Proteomic databases

PaxDbi O35385.
PRIDEi O35385.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000031359 ; ENSMUSP00000031359 ; ENSMUSG00000029410 .
GeneIDi 19023.
KEGGi mmu:19023.
UCSCi uc008yco.2. mouse.

Organism-specific databases

CTDi 5470.
MGIi MGI:1342304. Ppef2.

Phylogenomic databases

eggNOGi COG0639.
GeneTreei ENSGT00530000063173.
HOGENOMi HOG000006820.
HOVERGENi HBG008238.
InParanoidi O35385.
KOi K13807.
OMAi NMLEYKS.
OrthoDBi EOG7KH9J7.
PhylomeDBi O35385.
TreeFami TF313342.

Miscellaneous databases

NextBioi 295456.
PROi O35385.
SOURCEi Search...

Gene expression databases

Bgeei O35385.
Genevestigatori O35385.

Family and domain databases

Gene3Di 1.10.238.10. 2 hits.
3.60.21.10. 2 hits.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000048. IQ_motif_EF-hand-BS.
IPR029052. Metallo-depent_PP-like.
IPR013235. PPP_dom.
IPR012008. Ser/Thr-Pase_EF-hand_contain.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF13499. EF-hand_7. 1 hit.
PF00149. Metallophos. 1 hit.
PF08321. PPP5. 1 hit.
[Graphical view ]
PIRSFi PIRSF000912. PPEF. 1 hit.
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00054. EFh. 3 hits.
SM00015. IQ. 1 hit.
SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 2 hits.
PROSITEi PS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
PS50096. IQ. 1 hit.
PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of a conserved family of protein serine/threonine phosphatases homologous to Drosophila retinal degeneration C."
    Sherman P.M., Sun H., Macke J.P., Williams J., Smallwood P.M., Nathans J.
    Proc. Natl. Acad. Sci. U.S.A. 94:11639-11644(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Eye.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Retina.

Entry informationi

Entry nameiPPE2_MOUSE
AccessioniPrimary (citable) accession number: O35385
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 1, 1998
Last modified: July 9, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi