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Protein

Neuropilin-2

Gene

Nrp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

High affinity receptor for semaphorins 3C, 3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi197 – 1971CalciumBy similarity
Metal bindingi211 – 2111CalciumBy similarity
Metal bindingi252 – 2521CalciumBy similarity

GO - Molecular functioni

GO - Biological processi

  • angiogenesis Source: InterPro
  • axon extension involved in axon guidance Source: BHF-UCL
  • axon guidance Source: MGI
  • dorsal root ganglion morphogenesis Source: ParkinsonsUK-UCL
  • facial nerve structural organization Source: ParkinsonsUK-UCL
  • facioacoustic ganglion development Source: ParkinsonsUK-UCL
  • gonadotrophin-releasing hormone neuronal migration to the hypothalamus Source: BHF-UCL
  • heart development Source: MGI
  • negative chemotaxis Source: MGI
  • nerve development Source: BHF-UCL
  • neural crest cell migration Source: MGI
  • neural crest cell migration involved in autonomic nervous system development Source: ParkinsonsUK-UCL
  • neuron migration Source: BHF-UCL
  • semaphorin-plexin signaling pathway involved in neuron projection guidance Source: BHF-UCL
  • sensory neuron axon guidance Source: ParkinsonsUK-UCL
  • sympathetic ganglion development Source: BHF-UCL
  • sympathetic neuron projection extension Source: BHF-UCL
  • sympathetic neuron projection guidance Source: BHF-UCL
  • trigeminal ganglion development Source: ParkinsonsUK-UCL
  • ventral trunk neural crest cell migration Source: ParkinsonsUK-UCL
  • vestibulocochlear nerve structural organization Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Receptor

Keywords - Biological processi

Differentiation, Neurogenesis

Keywords - Ligandi

Calcium, Heparin-binding, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-194306. Neurophilin interactions with VEGF and VEGFR.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuropilin-2
Alternative name(s):
Vascular endothelial cell growth factor 165 receptor 2
Gene namesi
Name:Nrp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1100492. Nrp2.

Subcellular locationi

  • Membrane By similarity; Single-pass type I membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 864844ExtracellularSequence analysisAdd
BLAST
Transmembranei865 – 88925HelicalSequence analysisAdd
BLAST
Topological domaini890 – 93142CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • axon Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 931911Neuropilin-2PRO_0000021864Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi28 ↔ 55By similarity
Disulfide bondi83 ↔ 105By similarity
Disulfide bondi149 ↔ 175By similarity
Glycosylationi152 – 1521N-linked (GlcNAc...)Sequence analysis
Glycosylationi157 – 1571N-linked (GlcNAc...)Sequence analysis
Disulfide bondi208 ↔ 230By similarity
Disulfide bondi277 ↔ 427By similarity
Disulfide bondi434 ↔ 592By similarity
Glycosylationi629 – 6291N-linked (GlcNAc...)Sequence analysis
Glycosylationi839 – 8391N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO35375.
PaxDbiO35375.
PRIDEiO35375.

PTM databases

PhosphoSiteiO35375.
SwissPalmiO35375.

Expressioni

Tissue specificityi

Expressed in developing CNS, PNS and in some nonneural tissues including limb buds, developing bones, muscles, intestinal epithelium, kidney, lung and submandibular gland.

Developmental stagei

The expression pattern is very dynamic and is developmentally regulated.

Gene expression databases

BgeeiO35375.
CleanExiMM_NRP2.
ExpressionAtlasiO35375. baseline and differential.
GenevisibleiO35375. MM.

Interactioni

Subunit structurei

Heterodimer with NRP1. Binds PLXNB1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi201849. 1 interaction.
STRINGi10090.ENSMUSP00000109794.

Structurei

3D structure databases

ProteinModelPortaliO35375.
SMRiO35375. Positions 26-595.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 142115CUB 1PROSITE-ProRule annotationAdd
BLAST
Domaini149 – 267119CUB 2PROSITE-ProRule annotationAdd
BLAST
Domaini277 – 427151F5/8 type C 1PROSITE-ProRule annotationAdd
BLAST
Domaini434 – 592159F5/8 type C 2PROSITE-ProRule annotationAdd
BLAST
Domaini642 – 802161MAMPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi838 – 8458Poly-Ser

Domaini

The tandem CUB domains mediate binding to semaphorin, while the tandem F5/8 domains are responsible for heparin and VEGF binding.By similarity

Sequence similaritiesi

Belongs to the neuropilin family.Curated
Contains 2 CUB domains.PROSITE-ProRule annotation
Contains 2 F5/8 type C domains.PROSITE-ProRule annotation
Contains 1 MAM domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHB5. Eukaryota.
ENOG410ZPIE. LUCA.
GeneTreeiENSGT00760000119073.
HOVERGENiHBG000502.
InParanoidiO35375.
KOiK06819.
OMAiEYEVDWS.
OrthoDBiEOG7ZWD1D.
TreeFamiTF316506.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
2.60.120.290. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR000859. CUB_dom.
IPR000421. FA58C.
IPR008979. Galactose-bd-like.
IPR000998. MAM_dom.
IPR014648. Neuropilin.
IPR027143. Neuropilin-2.
IPR022579. Neuropilin_C.
[Graphical view]
PANTHERiPTHR10127:SF30. PTHR10127:SF30. 1 hit.
PfamiPF00431. CUB. 2 hits.
PF11980. DUF3481. 1 hit.
PF00754. F5_F8_type_C. 2 hits.
PF00629. MAM. 1 hit.
[Graphical view]
PIRSFiPIRSF036960. Neuropilin. 1 hit.
PRINTSiPR00020. MAMDOMAIN.
SMARTiSM00042. CUB. 2 hits.
SM00231. FA58C. 2 hits.
SM00137. MAM. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF49854. SSF49854. 2 hits.
SSF49899. SSF49899. 1 hit.
PROSITEiPS01180. CUB. 2 hits.
PS01285. FA58C_1. 2 hits.
PS01286. FA58C_2. 2 hits.
PS50022. FA58C_3. 2 hits.
PS50060. MAM_2. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A22 (identifier: O35375-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDMFPLTWVF LALYFSGHEV RSQQDPPCGG RLNSKDAGYI TSPGYPQDYP
60 70 80 90 100
SHQNCEWIVY APEPNQKIVL NFNPHFEIEK HDCKYDFIEI RDGDSESADL
110 120 130 140 150
LGKHCGNIAP PTIISSGSVL YIKFTSDYAR QGAGFSLRYE IFKTGSEDCS
160 170 180 190 200
KNFTSPNGTI ESPGFPEKYP HNLDCTFTIL AKPRMEIILQ FLTFDLEHDP
210 220 230 240 250
LQVGEGDCKY DWLDIWDGIP HVGPLIGKYC GTKTPSKLRS STGILSLTFH
260 270 280 290 300
TDMAVAKDGF SARYYLIHQE PPENFQCNVP LGMESGRIAN EQISASSTFS
310 320 330 340 350
DGRWTPQQSR LHGDDNGWTP NLDSNKEYLQ VDLRFLTMLT AIATQGAISR
360 370 380 390 400
ETQKGYYVKS YKLEVSTNGE DWMVYRHGKN HKIFQANNDA TEVVLNKLHM
410 420 430 440 450
PLLTRFIRIR PQTWHLGIAL RLELFGCRVT DAPCSNMLGM LSGLIADTQI
460 470 480 490 500
SASSTREYLW SPSAARLVSS RSGWFPRNPQ AQPGEEWLQV DLGTPKTVKG
510 520 530 540 550
VIIQGARGGD SITAVEARAF VRKFKVSYSL NGKDWEYIQD PRTQQTKLFE
560 570 580 590 600
GNMHYDTPDI RRFDPVPAQY VRVYPERWSP AGIGMRLEVL GCDWTDSKPT
610 620 630 640 650
VETLGPTVKS EETTTPYPMD EDATECGENC SFEDDKDLQL PSGFNCNFDF
660 670 680 690 700
PEETCGWVYD HAKWLRSTWI SSANPNDRTF PDDKNFLKLQ SDGRREGQYG
710 720 730 740 750
RLISPPVHLP RSPVCMEFQY QAMGGHGVAL QVVREASQES KLLWVIREDQ
760 770 780 790 800
GSEWKHGRII LPSYDMEYQI VFEGVIGKGR SGEISIDDIR ISTDVPLENC
810 820 830 840 850
MEPISAFAGE DFKVDIPETH GGEGYEDEID DEYEGDWSNS SSSTSGAGDP
860 870 880 890 900
SSGKEKSWLY TLDPILITII AMSSLGVLLG ATCAGLLLYC TCSYSGLSSR
910 920 930
SCTTLENYNF ELYDGLKHKV KINHQKCCSE A
Length:931
Mass (Da):104,631
Last modified:July 27, 2011 - v2
Checksum:i08AA821E696A2885
GO
Isoform A0 (identifier: O35375-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     809-830: Missing.

Show »
Length:909
Mass (Da):102,198
Checksum:iA34352A52526A136
GO
Isoform A5 (identifier: O35375-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     814-830: Missing.

Show »
Length:914
Mass (Da):102,775
Checksum:i6EA486DAB152F20B
GO
Isoform A17 (identifier: O35375-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     809-813: Missing.

Show »
Length:926
Mass (Da):104,055
Checksum:i8073D6478C9A8467
GO
Isoform B0 (identifier: O35375-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     810-931: EDFKVDIPET...INHQKCCSEA → GTLPPGTEPT...KLEQERGSHC

Show »
Length:901
Mass (Da):101,418
Checksum:iF763734C2A09F52F
GO
Isoform B5 (identifier: O35375-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     814-931: VDIPETHGGE...INHQKCCSEA → GGTLPPGTEP...KLEQERGSHC

Show »
Length:906
Mass (Da):101,995
Checksum:i6D66AF632D47A21F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti32 – 321L → P in AAC53377 (PubMed:9331348).Curated
Sequence conflicti32 – 321L → P in AAC53378 (PubMed:9331348).Curated
Sequence conflicti32 – 321L → P in AAC53379 (PubMed:9331348).Curated
Sequence conflicti32 – 321L → P in AAC53380 (PubMed:9331348).Curated
Sequence conflicti32 – 321L → P in AAC53381 (PubMed:9331348).Curated
Sequence conflicti32 – 321L → P in AAC53382 (PubMed:9331348).Curated
Sequence conflicti786 – 7861I → G in AAC53377 (PubMed:9331348).Curated
Sequence conflicti786 – 7861I → G in AAC53378 (PubMed:9331348).Curated
Sequence conflicti786 – 7861I → G in AAC53382 (PubMed:9331348).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei809 – 83022Missing in isoform A0. 1 PublicationVSP_004344Add
BLAST
Alternative sequencei809 – 8135Missing in isoform A17. 1 PublicationVSP_004343
Alternative sequencei810 – 931122EDFKV…CCSEA → GTLPPGTEPTVDTVPVQPIP AYWYYVMAAGGAVLVLASVV LALVLHYHRFRYAAKKTDHS ITYKTSHYTNGAPLAVEPTL TIKLEQERGSHC in isoform B0. 1 PublicationVSP_004346Add
BLAST
Alternative sequencei814 – 931118VDIPE…CCSEA → GGTLPPGTEPTVDTVPVQPI PAYWYYVMAAGGAVLVLASV VLALVLHYHRFRYAAKKTDH SITYKTSHYTNGAPLAVEPT LTIKLEQERGSHC in isoform B5. 1 PublicationVSP_004347Add
BLAST
Alternative sequencei814 – 83017Missing in isoform A5. 1 PublicationVSP_004345Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF022856 mRNA. Translation: AAC53379.1.
AF022854 mRNA. Translation: AAC53377.1.
AF022855 mRNA. Translation: AAC53378.1.
AF022857 mRNA. Translation: AAC53380.1.
AF022858 mRNA. Translation: AAC53381.1.
AF022861 mRNA. Translation: AAC53382.1.
AL671560, AL645727 Genomic DNA. Translation: CAI24156.1.
AL645727, AL671560 Genomic DNA. Translation: CAI24511.1.
CCDSiCCDS14995.1. [O35375-3]
CCDS35589.1. [O35375-1]
CCDS35590.1. [O35375-4]
CCDS35591.1. [O35375-2]
CCDS35592.1. [O35375-6]
CCDS35593.1. [O35375-5]
RefSeqiNP_001070871.1. NM_001077403.1. [O35375-1]
UniGeneiMm.266341.

Genome annotation databases

EnsembliENSMUST00000027112; ENSMUSP00000027112; ENSMUSG00000025969. [O35375-6]
ENSMUST00000063594; ENSMUSP00000069379; ENSMUSG00000025969. [O35375-4]
ENSMUST00000075144; ENSMUSP00000074642; ENSMUSG00000025969. [O35375-3]
ENSMUST00000102822; ENSMUSP00000099886; ENSMUSG00000025969. [O35375-2]
ENSMUST00000114155; ENSMUSP00000109792; ENSMUSG00000025969. [O35375-5]
ENSMUST00000114157; ENSMUSP00000109794; ENSMUSG00000025969. [O35375-1]
GeneIDi18187.
KEGGimmu:18187.
UCSCiuc007bfj.1. mouse. [O35375-6]
uc007bfl.1. mouse. [O35375-1]
uc007bfn.1. mouse. [O35375-3]
uc007bfo.1. mouse. [O35375-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF022856 mRNA. Translation: AAC53379.1.
AF022854 mRNA. Translation: AAC53377.1.
AF022855 mRNA. Translation: AAC53378.1.
AF022857 mRNA. Translation: AAC53380.1.
AF022858 mRNA. Translation: AAC53381.1.
AF022861 mRNA. Translation: AAC53382.1.
AL671560, AL645727 Genomic DNA. Translation: CAI24156.1.
AL645727, AL671560 Genomic DNA. Translation: CAI24511.1.
CCDSiCCDS14995.1. [O35375-3]
CCDS35589.1. [O35375-1]
CCDS35590.1. [O35375-4]
CCDS35591.1. [O35375-2]
CCDS35592.1. [O35375-6]
CCDS35593.1. [O35375-5]
RefSeqiNP_001070871.1. NM_001077403.1. [O35375-1]
UniGeneiMm.266341.

3D structure databases

ProteinModelPortaliO35375.
SMRiO35375. Positions 26-595.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201849. 1 interaction.
STRINGi10090.ENSMUSP00000109794.

PTM databases

PhosphoSiteiO35375.
SwissPalmiO35375.

Proteomic databases

MaxQBiO35375.
PaxDbiO35375.
PRIDEiO35375.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027112; ENSMUSP00000027112; ENSMUSG00000025969. [O35375-6]
ENSMUST00000063594; ENSMUSP00000069379; ENSMUSG00000025969. [O35375-4]
ENSMUST00000075144; ENSMUSP00000074642; ENSMUSG00000025969. [O35375-3]
ENSMUST00000102822; ENSMUSP00000099886; ENSMUSG00000025969. [O35375-2]
ENSMUST00000114155; ENSMUSP00000109792; ENSMUSG00000025969. [O35375-5]
ENSMUST00000114157; ENSMUSP00000109794; ENSMUSG00000025969. [O35375-1]
GeneIDi18187.
KEGGimmu:18187.
UCSCiuc007bfj.1. mouse. [O35375-6]
uc007bfl.1. mouse. [O35375-1]
uc007bfn.1. mouse. [O35375-3]
uc007bfo.1. mouse. [O35375-2]

Organism-specific databases

CTDi8828.
MGIiMGI:1100492. Nrp2.

Phylogenomic databases

eggNOGiENOG410IHB5. Eukaryota.
ENOG410ZPIE. LUCA.
GeneTreeiENSGT00760000119073.
HOVERGENiHBG000502.
InParanoidiO35375.
KOiK06819.
OMAiEYEVDWS.
OrthoDBiEOG7ZWD1D.
TreeFamiTF316506.

Enzyme and pathway databases

ReactomeiR-MMU-194306. Neurophilin interactions with VEGF and VEGFR.

Miscellaneous databases

ChiTaRSiNrp2. mouse.
NextBioi293510.
PROiO35375.
SOURCEiSearch...

Gene expression databases

BgeeiO35375.
CleanExiMM_NRP2.
ExpressionAtlasiO35375. baseline and differential.
GenevisibleiO35375. MM.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
2.60.120.290. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR000859. CUB_dom.
IPR000421. FA58C.
IPR008979. Galactose-bd-like.
IPR000998. MAM_dom.
IPR014648. Neuropilin.
IPR027143. Neuropilin-2.
IPR022579. Neuropilin_C.
[Graphical view]
PANTHERiPTHR10127:SF30. PTHR10127:SF30. 1 hit.
PfamiPF00431. CUB. 2 hits.
PF11980. DUF3481. 1 hit.
PF00754. F5_F8_type_C. 2 hits.
PF00629. MAM. 1 hit.
[Graphical view]
PIRSFiPIRSF036960. Neuropilin. 1 hit.
PRINTSiPR00020. MAMDOMAIN.
SMARTiSM00042. CUB. 2 hits.
SM00231. FA58C. 2 hits.
SM00137. MAM. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF49854. SSF49854. 2 hits.
SSF49899. SSF49899. 1 hit.
PROSITEiPS01180. CUB. 2 hits.
PS01285. FA58C_1. 2 hits.
PS01286. FA58C_2. 2 hits.
PS50022. FA58C_3. 2 hits.
PS50060. MAM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Neuropilin-2, a novel member of the neuropilin family, is a high affinity receptor for the semaphorins Sema E and Sema IV but not Sema III."
    Chen H., Chedotal A., He Z.-G., Goodman C.S., Tessier-Lavigne M.
    Neuron 19:547-559(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A0; A17; A22; A5; B0 AND B5).
    Strain: BALB/cJ.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney and Lung.

Entry informationi

Entry nameiNRP2_MOUSE
AccessioniPrimary (citable) accession number: O35375
Secondary accession number(s): B1AT44
, O35373, O35374, O35376, O35377, O35378
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.