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Protein

Neuropilin-2

Gene

Nrp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

High affinity receptor for semaphorins 3C, 3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi197CalciumBy similarity1
Metal bindingi211CalciumBy similarity1
Metal bindingi252CalciumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • angiogenesis Source: InterPro
  • axon extension involved in axon guidance Source: BHF-UCL
  • axon guidance Source: MGI
  • cellular response to leukemia inhibitory factor Source: MGI
  • dorsal root ganglion morphogenesis Source: ParkinsonsUK-UCL
  • facial nerve structural organization Source: ParkinsonsUK-UCL
  • facioacoustic ganglion development Source: ParkinsonsUK-UCL
  • gonadotrophin-releasing hormone neuronal migration to the hypothalamus Source: BHF-UCL
  • heart development Source: MGI
  • negative chemotaxis Source: MGI
  • nerve development Source: BHF-UCL
  • neural crest cell migration Source: MGI
  • neural crest cell migration involved in autonomic nervous system development Source: ParkinsonsUK-UCL
  • neuron migration Source: BHF-UCL
  • outflow tract septum morphogenesis Source: BHF-UCL
  • semaphorin-plexin signaling pathway involved in neuron projection guidance Source: BHF-UCL
  • sensory neuron axon guidance Source: ParkinsonsUK-UCL
  • sympathetic ganglion development Source: BHF-UCL
  • sympathetic neuron projection extension Source: BHF-UCL
  • sympathetic neuron projection guidance Source: BHF-UCL
  • trigeminal ganglion development Source: ParkinsonsUK-UCL
  • ventral trunk neural crest cell migration Source: ParkinsonsUK-UCL
  • vestibulocochlear nerve structural organization Source: ParkinsonsUK-UCL

Keywordsi

Molecular functionDevelopmental protein, Heparin-binding, Receptor
Biological processDifferentiation, Neurogenesis
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-194306. Neurophilin interactions with VEGF and VEGFR.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuropilin-2
Alternative name(s):
Vascular endothelial cell growth factor 165 receptor 2
Gene namesi
Name:Nrp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1100492. Nrp2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 864ExtracellularSequence analysisAdd BLAST844
Transmembranei865 – 889HelicalSequence analysisAdd BLAST25
Topological domaini890 – 931CytoplasmicSequence analysisAdd BLAST42

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000002186421 – 931Neuropilin-2Add BLAST911

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi28 ↔ 55By similarity
Disulfide bondi83 ↔ 105By similarity
Disulfide bondi149 ↔ 175By similarity
Glycosylationi152N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi157N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi208 ↔ 230By similarity
Disulfide bondi277 ↔ 427By similarity
Disulfide bondi434 ↔ 592By similarity
Glycosylationi629N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi839N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO35375.
PaxDbiO35375.
PeptideAtlasiO35375.
PRIDEiO35375.

PTM databases

iPTMnetiO35375.
PhosphoSitePlusiO35375.
SwissPalmiO35375.

Expressioni

Tissue specificityi

Expressed in developing CNS, PNS and in some nonneural tissues including limb buds, developing bones, muscles, intestinal epithelium, kidney, lung and submandibular gland.

Developmental stagei

The expression pattern is very dynamic and is developmentally regulated.

Gene expression databases

BgeeiENSMUSG00000025969.
CleanExiMM_NRP2.
ExpressionAtlasiO35375. baseline and differential.
GenevisibleiO35375. MM.

Interactioni

Subunit structurei

Heterodimer with NRP1. Binds PLXNB1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi201849. 1 interactor.
STRINGi10090.ENSMUSP00000109794.

Structurei

3D structure databases

ProteinModelPortaliO35375.
SMRiO35375.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 142CUB 1PROSITE-ProRule annotationAdd BLAST115
Domaini149 – 267CUB 2PROSITE-ProRule annotationAdd BLAST119
Domaini277 – 427F5/8 type C 1PROSITE-ProRule annotationAdd BLAST151
Domaini434 – 592F5/8 type C 2PROSITE-ProRule annotationAdd BLAST159
Domaini642 – 802MAMPROSITE-ProRule annotationAdd BLAST161

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi838 – 845Poly-Ser8

Domaini

The tandem CUB domains mediate binding to semaphorin, while the tandem F5/8 domains are responsible for heparin and VEGF binding.By similarity

Sequence similaritiesi

Belongs to the neuropilin family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHB5. Eukaryota.
ENOG410ZPIE. LUCA.
GeneTreeiENSGT00760000119073.
HOVERGENiHBG000502.
InParanoidiO35375.
KOiK06819.
OMAiEYEVDWS.
OrthoDBiEOG091G01LI.
TreeFamiTF316506.

Family and domain databases

CDDicd00041. CUB. 2 hits.
cd06263. MAM. 1 hit.
Gene3Di2.60.120.260. 2 hits.
2.60.120.290. 2 hits.
InterProiView protein in InterPro
IPR013320. ConA-like_dom_sf.
IPR000859. CUB_dom.
IPR000421. FA58C.
IPR008979. Galactose-bd-like_sf.
IPR000998. MAM_dom.
IPR014648. Neuropilin.
IPR027143. Neuropilin-2.
IPR022579. Neuropilin_C.
IPR035914. Sperma_CUB_dom_sf.
PANTHERiPTHR44185:SF2. PTHR44185:SF2. 1 hit.
PfamiView protein in Pfam
PF00431. CUB. 2 hits.
PF11980. DUF3481. 1 hit.
PF00754. F5_F8_type_C. 2 hits.
PF00629. MAM. 1 hit.
PIRSFiPIRSF036960. Neuropilin. 1 hit.
PRINTSiPR00020. MAMDOMAIN.
SMARTiView protein in SMART
SM00042. CUB. 2 hits.
SM00231. FA58C. 2 hits.
SM00137. MAM. 1 hit.
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF49854. SSF49854. 2 hits.
SSF49899. SSF49899. 1 hit.
PROSITEiView protein in PROSITE
PS01180. CUB. 2 hits.
PS01285. FA58C_1. 2 hits.
PS01286. FA58C_2. 2 hits.
PS50022. FA58C_3. 2 hits.
PS50060. MAM_2. 1 hit.

Sequences (6)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A22 (identifier: O35375-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDMFPLTWVF LALYFSGHEV RSQQDPPCGG RLNSKDAGYI TSPGYPQDYP
60 70 80 90 100
SHQNCEWIVY APEPNQKIVL NFNPHFEIEK HDCKYDFIEI RDGDSESADL
110 120 130 140 150
LGKHCGNIAP PTIISSGSVL YIKFTSDYAR QGAGFSLRYE IFKTGSEDCS
160 170 180 190 200
KNFTSPNGTI ESPGFPEKYP HNLDCTFTIL AKPRMEIILQ FLTFDLEHDP
210 220 230 240 250
LQVGEGDCKY DWLDIWDGIP HVGPLIGKYC GTKTPSKLRS STGILSLTFH
260 270 280 290 300
TDMAVAKDGF SARYYLIHQE PPENFQCNVP LGMESGRIAN EQISASSTFS
310 320 330 340 350
DGRWTPQQSR LHGDDNGWTP NLDSNKEYLQ VDLRFLTMLT AIATQGAISR
360 370 380 390 400
ETQKGYYVKS YKLEVSTNGE DWMVYRHGKN HKIFQANNDA TEVVLNKLHM
410 420 430 440 450
PLLTRFIRIR PQTWHLGIAL RLELFGCRVT DAPCSNMLGM LSGLIADTQI
460 470 480 490 500
SASSTREYLW SPSAARLVSS RSGWFPRNPQ AQPGEEWLQV DLGTPKTVKG
510 520 530 540 550
VIIQGARGGD SITAVEARAF VRKFKVSYSL NGKDWEYIQD PRTQQTKLFE
560 570 580 590 600
GNMHYDTPDI RRFDPVPAQY VRVYPERWSP AGIGMRLEVL GCDWTDSKPT
610 620 630 640 650
VETLGPTVKS EETTTPYPMD EDATECGENC SFEDDKDLQL PSGFNCNFDF
660 670 680 690 700
PEETCGWVYD HAKWLRSTWI SSANPNDRTF PDDKNFLKLQ SDGRREGQYG
710 720 730 740 750
RLISPPVHLP RSPVCMEFQY QAMGGHGVAL QVVREASQES KLLWVIREDQ
760 770 780 790 800
GSEWKHGRII LPSYDMEYQI VFEGVIGKGR SGEISIDDIR ISTDVPLENC
810 820 830 840 850
MEPISAFAGE DFKVDIPETH GGEGYEDEID DEYEGDWSNS SSSTSGAGDP
860 870 880 890 900
SSGKEKSWLY TLDPILITII AMSSLGVLLG ATCAGLLLYC TCSYSGLSSR
910 920 930
SCTTLENYNF ELYDGLKHKV KINHQKCCSE A
Length:931
Mass (Da):104,631
Last modified:July 27, 2011 - v2
Checksum:i08AA821E696A2885
GO
Isoform A0 (identifier: O35375-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     809-830: Missing.

Show »
Length:909
Mass (Da):102,198
Checksum:iA34352A52526A136
GO
Isoform A5 (identifier: O35375-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     814-830: Missing.

Show »
Length:914
Mass (Da):102,775
Checksum:i6EA486DAB152F20B
GO
Isoform A17 (identifier: O35375-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     809-813: Missing.

Show »
Length:926
Mass (Da):104,055
Checksum:i8073D6478C9A8467
GO
Isoform B0 (identifier: O35375-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     810-931: EDFKVDIPET...INHQKCCSEA → GTLPPGTEPT...KLEQERGSHC

Show »
Length:901
Mass (Da):101,418
Checksum:iF763734C2A09F52F
GO
Isoform B5 (identifier: O35375-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     814-931: VDIPETHGGE...INHQKCCSEA → GGTLPPGTEP...KLEQERGSHC

Show »
Length:906
Mass (Da):101,995
Checksum:i6D66AF632D47A21F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti32L → P in AAC53377 (PubMed:9331348).Curated1
Sequence conflicti32L → P in AAC53378 (PubMed:9331348).Curated1
Sequence conflicti32L → P in AAC53379 (PubMed:9331348).Curated1
Sequence conflicti32L → P in AAC53380 (PubMed:9331348).Curated1
Sequence conflicti32L → P in AAC53381 (PubMed:9331348).Curated1
Sequence conflicti32L → P in AAC53382 (PubMed:9331348).Curated1
Sequence conflicti786I → G in AAC53377 (PubMed:9331348).Curated1
Sequence conflicti786I → G in AAC53378 (PubMed:9331348).Curated1
Sequence conflicti786I → G in AAC53382 (PubMed:9331348).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004344809 – 830Missing in isoform A0. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_004343809 – 813Missing in isoform A17. 1 Publication5
Alternative sequenceiVSP_004346810 – 931EDFKV…CCSEA → GTLPPGTEPTVDTVPVQPIP AYWYYVMAAGGAVLVLASVV LALVLHYHRFRYAAKKTDHS ITYKTSHYTNGAPLAVEPTL TIKLEQERGSHC in isoform B0. 1 PublicationAdd BLAST122
Alternative sequenceiVSP_004347814 – 931VDIPE…CCSEA → GGTLPPGTEPTVDTVPVQPI PAYWYYVMAAGGAVLVLASV VLALVLHYHRFRYAAKKTDH SITYKTSHYTNGAPLAVEPT LTIKLEQERGSHC in isoform B5. 1 PublicationAdd BLAST118
Alternative sequenceiVSP_004345814 – 830Missing in isoform A5. 1 PublicationAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF022856 mRNA. Translation: AAC53379.1.
AF022854 mRNA. Translation: AAC53377.1.
AF022855 mRNA. Translation: AAC53378.1.
AF022857 mRNA. Translation: AAC53380.1.
AF022858 mRNA. Translation: AAC53381.1.
AF022861 mRNA. Translation: AAC53382.1.
AL671560, AL645727 Genomic DNA. Translation: CAI24156.1.
AL645727, AL671560 Genomic DNA. Translation: CAI24511.1.
CCDSiCCDS14995.1. [O35375-3]
CCDS35589.1. [O35375-1]
CCDS35590.1. [O35375-4]
CCDS35591.1. [O35375-2]
CCDS35592.1. [O35375-6]
CCDS35593.1. [O35375-5]
RefSeqiNP_001070871.1. NM_001077403.1. [O35375-1]
UniGeneiMm.266341.

Genome annotation databases

EnsembliENSMUST00000027112; ENSMUSP00000027112; ENSMUSG00000025969. [O35375-6]
ENSMUST00000063594; ENSMUSP00000069379; ENSMUSG00000025969. [O35375-4]
ENSMUST00000075144; ENSMUSP00000074642; ENSMUSG00000025969. [O35375-3]
ENSMUST00000102822; ENSMUSP00000099886; ENSMUSG00000025969. [O35375-2]
ENSMUST00000114155; ENSMUSP00000109792; ENSMUSG00000025969. [O35375-5]
ENSMUST00000114157; ENSMUSP00000109794; ENSMUSG00000025969. [O35375-1]
GeneIDi18187.
KEGGimmu:18187.
UCSCiuc007bfj.1. mouse. [O35375-6]
uc007bfl.1. mouse. [O35375-1]
uc007bfn.1. mouse. [O35375-3]
uc007bfo.1. mouse. [O35375-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNRP2_MOUSE
AccessioniPrimary (citable) accession number: O35375
Secondary accession number(s): B1AT44
, O35373, O35374, O35376, O35377, O35378
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: November 22, 2017
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families