O35367 (KERA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Keratocan
Short name=KTN

Alternative name(s):
Keratan sulfate proteoglycan keratocan

Gene names

Name:	Kera
Synonyms:	Ktcn

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be important in developing and maintaining corneal transparency and for the structure of the stromal matrix.
Subcellular location	Secreted › extracellular space › extracellular matrix By similarity.
Tissue specificity	Selectively expressed in cornea of adult where it is detected in keratocytes but not in scleral cells. In embryo, first detected in periocular mesenchymal cells migrating toward developing cornea on E13.5; expression gradually restricted to corneal stromal cells on E14.5 to E18.5. Detected in scleral cells of E15.5 but not in E18.5 embryos.
Post-translational modification	Binds keratan sulfate chains.
Sequence similarities	Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily. Contains 10 LRR (leucine-rich) repeats. Contains 1 LRRNT domain.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Domain	Leucine-rich repeat Repeat Signal
PTM	Disulfide bond Glycoprotein Proteoglycan
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cornea development in camera-type eye Inferred from mutant phenotype PubMed 10802664. Source: MGI
Cellular_component	proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 351	331	Keratocan		PRO_0000032749
Regions
	Domain	34 – 72	39	LRRNT
	Repeat	73 – 94	22	LRR 1
	Repeat	97 – 118	22	LRR 2
	Repeat	123 – 143	21	LRR 3
	Repeat	144 – 165	22	LRR 4
	Repeat	168 – 181	14	LRR 5
	Repeat	194 – 214	21	LRR 6
	Repeat	215 – 236	22	LRR 7
	Repeat	239 – 259	21	LRR 8
	Repeat	264 – 283	20	LRR 9
	Repeat	284 – 305	22	LRR 10
	Compositional bias	42 – 58	17	Cys-rich
Amino acid modifications
	Glycosylation	94	1	N-linked (GlcNAc...) (keratan sulfate) By similarity
	Glycosylation	168	1	N-linked (GlcNAc...) (keratan sulfate) By similarity
	Glycosylation	223	1	N-linked (GlcNAc...) Potential
	Glycosylation	299	1	N-linked (GlcNAc...) Potential
	Disulfide bond	304 ↔ 342		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O35367 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: B0632B387864B98D	FASTA	351	40,404
	10 20 30 40 50 60 MATPNCLILW VLLIADTVWT QSVRQAYEIQ DPEDWDVHDD FYCPRECFCP PSFPTALYCE 70 80 90 100 110 120 NRGLTEIPPI PSRIWYLYLE NNLIESIPEK PFENATQLRW INLNKNKITN YGIEKGALSQ 130 140 150 160 170 180 LKKLLFLFLE DNELEEVPSP LPRSLEQLQL ARNKVSRIPQ GTFSNLENLT LLDLQHNKLL 190 200 210 220 230 240 DNAFQRDTFK GLKNLMQLNM AKNALRNMPP RLPANTMQLF LDNNSIEGIP ENYFNVIPKV 250 260 270 280 290 300 AFLRLNHNKL SDAGLPSRGF DVSSILDLQL SYNQLTNFPR INANLQHLHL DHNKIKNVNM 310 320 330 340 350 SVICPTTLRA EQDAFIHGPQ LSYLRLDGNE IKPPIPIDLV ACFKLLQAFI I

References

[1]	"Cloning and chromosomal localization of mouse keratocan, a corneal keratan sulfate proteoglycan." Dunlevy J.R., Chakravarti S., Gyalzen P., Vergnes J.-P., Hassell J.R. Mamm. Genome 9:316-319(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: AKR/J X C57L/J. Tissue: Eye.
[2]	"The cloning of mouse keratocan cDNA and genomic DNA and the characterization of its expression during eye development." Liu C.-Y., Shiraishi A., Kao C.W.-C., Converse R.L., Funderburgh J.L., Corpuz L.M., Conrad G.W., Kao W.W.-Y. J. Biol. Chem. 273:22584-22588(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ. Tissue: Cornea.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF022256 mRNA. Translation: AAC15505.1. AF057301 Genomic DNA. Translation: AAC61257.1.
IPI	IPI00985653.
RefSeq	NP_032464.1. NM_008438.3.
UniGene	Mm.6228.
3D structure databases
ProteinModelPortal	O35367.
SMR	O35367. Positions 41-336.
ModBase	Search...
PTM databases
PhosphoSite	O35367.
Proteomic databases
PRIDE	O35367.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000105286; ENSMUSP00000100923; ENSMUSG00000019932.
GeneID	16545.
KEGG	mmu:16545.
Organism-specific databases
CTD	11081.
MGI	MGI:1202398. Kera.
Phylogenomic databases
eggNOG	NOG278287.
GeneTree	ENSGT00600000084286.
HOVERGEN	HBG108061.
InParanoid	O35367.
KO	K08123.
OMA	RWINLNK.
OrthoDB	EOG4KSPK3.
Gene expression databases
ArrayExpress	O35367.
Bgee	O35367.
CleanEx	MM_KERA.
Genevestigator	O35367.
GermOnline	ENSMUSG00000019932. Mus musculus.
Family and domain databases
InterPro	IPR001611. Leu-rich_rpt. IPR000372. LRR-contain_N. [Graphical view]
Pfam	PF01462. LRRNT. 1 hit. [Graphical view]
SMART	SM00013. LRRNT. 1 hit. [Graphical view]
PROSITE	PS51450. LRR. 10 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	290011.
SOURCE	Search...

Entry information

Entry name

KERA_MOUSE

Accession

Primary (citable) accession number: O35367

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 11, 2002
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program