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Protein

Keratocan

Gene

Kera

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May be important in developing and maintaining corneal transparency and for the structure of the stromal matrix.

GO - Biological processi

  1. cornea development in camera-type eye Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_291717. Defective B4GALT1 causes B4GALT1-CDG (CDG-2d).
REACT_309433. Keratan sulfate degradation.
REACT_342477. Defective CHST6 causes MCDC1.
REACT_346228. Keratan sulfate biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Keratocan
Short name:
KTN
Alternative name(s):
Keratan sulfate proteoglycan keratocan
Gene namesi
Name:Kera
Synonyms:Ktcn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1202398. Kera.

Subcellular locationi

GO - Cellular componenti

  1. proteinaceous extracellular matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 351331KeratocanPRO_0000032749Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi94 – 941N-linked (GlcNAc...) (keratan sulfate)By similarity
Glycosylationi168 – 1681N-linked (GlcNAc...) (keratan sulfate)By similarity
Glycosylationi223 – 2231N-linked (GlcNAc...)Sequence Analysis
Glycosylationi299 – 2991N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi304 ↔ 342By similarity

Post-translational modificationi

Binds keratan sulfate chains.

Keywords - PTMi

Disulfide bond, Glycoprotein, Proteoglycan

Proteomic databases

MaxQBiO35367.
PRIDEiO35367.

PTM databases

PhosphoSiteiO35367.

Expressioni

Tissue specificityi

Selectively expressed in cornea of adult where it is detected in keratocytes but not in scleral cells. In embryo, first detected in periocular mesenchymal cells migrating toward developing cornea on E13.5; expression gradually restricted to corneal stromal cells on E14.5 to E18.5. Detected in scleral cells of E15.5 but not in E18.5 embryos.

Gene expression databases

BgeeiO35367.
CleanExiMM_KERA.
ExpressionAtlasiO35367. baseline and differential.
GenevestigatoriO35367.

Structurei

3D structure databases

ProteinModelPortaliO35367.
SMRiO35367. Positions 16-336.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 7239LRRNTAdd
BLAST
Repeati73 – 9422LRR 1Add
BLAST
Repeati97 – 11822LRR 2Add
BLAST
Repeati123 – 14321LRR 3Add
BLAST
Repeati144 – 16522LRR 4Add
BLAST
Repeati168 – 18114LRR 5Add
BLAST
Repeati194 – 21421LRR 6Add
BLAST
Repeati215 – 23622LRR 7Add
BLAST
Repeati239 – 25921LRR 8Add
BLAST
Repeati264 – 28320LRR 9Add
BLAST
Repeati284 – 30522LRR 10Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi42 – 5817Cys-richAdd
BLAST

Sequence similaritiesi

Contains 10 LRR (leucine-rich) repeats.Curated
Contains 1 LRRNT domain.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal

Phylogenomic databases

eggNOGiNOG278287.
GeneTreeiENSGT00760000118969.
HOVERGENiHBG108061.
InParanoidiO35367.
KOiK08123.
OMAiMWVLFIT.
OrthoDBiEOG741Z2B.
PhylomeDBiO35367.
TreeFamiTF334562.

Family and domain databases

InterProiIPR001611. Leu-rich_rpt.
IPR000372. LRR-contain_N.
[Graphical view]
PfamiPF13855. LRR_8. 2 hits.
PF01462. LRRNT. 1 hit.
[Graphical view]
SMARTiSM00013. LRRNT. 1 hit.
[Graphical view]
PROSITEiPS51450. LRR. 10 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35367-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATPNCLILW VLLIADTVWT QSVRQAYEIQ DPEDWDVHDD FYCPRECFCP
60 70 80 90 100
PSFPTALYCE NRGLTEIPPI PSRIWYLYLE NNLIESIPEK PFENATQLRW
110 120 130 140 150
INLNKNKITN YGIEKGALSQ LKKLLFLFLE DNELEEVPSP LPRSLEQLQL
160 170 180 190 200
ARNKVSRIPQ GTFSNLENLT LLDLQHNKLL DNAFQRDTFK GLKNLMQLNM
210 220 230 240 250
AKNALRNMPP RLPANTMQLF LDNNSIEGIP ENYFNVIPKV AFLRLNHNKL
260 270 280 290 300
SDAGLPSRGF DVSSILDLQL SYNQLTNFPR INANLQHLHL DHNKIKNVNM
310 320 330 340 350
SVICPTTLRA EQDAFIHGPQ LSYLRLDGNE IKPPIPIDLV ACFKLLQAFI

I
Length:351
Mass (Da):40,404
Last modified:December 31, 1997 - v1
Checksum:iB0632B387864B98D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF022256 mRNA. Translation: AAC15505.1.
AF057301 Genomic DNA. Translation: AAC61257.1.
CCDSiCCDS36044.1.
RefSeqiNP_032464.1. NM_008438.3.
XP_006513336.1. XM_006513273.2.
UniGeneiMm.6228.

Genome annotation databases

EnsembliENSMUST00000105286; ENSMUSP00000100923; ENSMUSG00000019932.
GeneIDi16545.
KEGGimmu:16545.
UCSCiuc007gxa.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF022256 mRNA. Translation: AAC15505.1.
AF057301 Genomic DNA. Translation: AAC61257.1.
CCDSiCCDS36044.1.
RefSeqiNP_032464.1. NM_008438.3.
XP_006513336.1. XM_006513273.2.
UniGeneiMm.6228.

3D structure databases

ProteinModelPortaliO35367.
SMRiO35367. Positions 16-336.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiO35367.

Proteomic databases

MaxQBiO35367.
PRIDEiO35367.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000105286; ENSMUSP00000100923; ENSMUSG00000019932.
GeneIDi16545.
KEGGimmu:16545.
UCSCiuc007gxa.1. mouse.

Organism-specific databases

CTDi11081.
MGIiMGI:1202398. Kera.

Phylogenomic databases

eggNOGiNOG278287.
GeneTreeiENSGT00760000118969.
HOVERGENiHBG108061.
InParanoidiO35367.
KOiK08123.
OMAiMWVLFIT.
OrthoDBiEOG741Z2B.
PhylomeDBiO35367.
TreeFamiTF334562.

Enzyme and pathway databases

ReactomeiREACT_291717. Defective B4GALT1 causes B4GALT1-CDG (CDG-2d).
REACT_309433. Keratan sulfate degradation.
REACT_342477. Defective CHST6 causes MCDC1.
REACT_346228. Keratan sulfate biosynthesis.

Miscellaneous databases

NextBioi290011.
PROiO35367.
SOURCEiSearch...

Gene expression databases

BgeeiO35367.
CleanExiMM_KERA.
ExpressionAtlasiO35367. baseline and differential.
GenevestigatoriO35367.

Family and domain databases

InterProiIPR001611. Leu-rich_rpt.
IPR000372. LRR-contain_N.
[Graphical view]
PfamiPF13855. LRR_8. 2 hits.
PF01462. LRRNT. 1 hit.
[Graphical view]
SMARTiSM00013. LRRNT. 1 hit.
[Graphical view]
PROSITEiPS51450. LRR. 10 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and chromosomal localization of mouse keratocan, a corneal keratan sulfate proteoglycan."
    Dunlevy J.R., Chakravarti S., Gyalzen P., Vergnes J.-P., Hassell J.R.
    Mamm. Genome 9:316-319(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: AKR/J X C57L/J.
    Tissue: Eye.
  2. "The cloning of mouse keratocan cDNA and genomic DNA and the characterization of its expression during eye development."
    Liu C.-Y., Shiraishi A., Kao C.W.-C., Converse R.L., Funderburgh J.L., Corpuz L.M., Conrad G.W., Kao W.W.-Y.
    J. Biol. Chem. 273:22584-22588(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.
    Tissue: Cornea.

Entry informationi

Entry nameiKERA_MOUSE
AccessioniPrimary (citable) accession number: O35367
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2002
Last sequence update: December 31, 1997
Last modified: March 31, 2015
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.