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Protein

Calpain-1 catalytic subunit

Gene

Capn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.By similarity

Catalytic activityi

Broad endopeptidase specificity.By similarity

Cofactori

Ca2+By similarityNote: Binds 4 Ca2+ ions.By similarity

Enzyme regulationi

Activated by micromolar concentrations of calcium and inhibited by calpastatin.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei115By similarity1
Active sitei272By similarity1
Active sitei296By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi99 – 1061PROSITE-ProRule annotation8
Calcium bindingi302 – 3332PROSITE-ProRule annotationAdd BLAST32
Calcium bindingi597 – 6083PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi627 – 6384PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

  • mammary gland involution Source: MGI
  • proteolysis Source: MGI
  • receptor catabolic process Source: MGI
  • regulation of catalytic activity Source: UniProtKB
  • self proteolysis Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1474228 Degradation of the extracellular matrix
R-MMU-6798695 Neutrophil degranulation
R-MMU-6809371 Formation of the cornified envelope

Protein family/group databases

MEROPSiC02.001

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-1 catalytic subunitCurated (EC:3.4.22.52By similarity)
Alternative name(s):
Calcium-activated neutral proteinase 1By similarity
Short name:
CANP 1By similarity
Calpain mu-typeBy similarity
Calpain-1 large subunitBy similarity
Micromolar-calpain
Short name:
muCANPBy similarity
Gene namesi
Name:Capn1Imported
Synonyms:Canp1, Capa1By similarity
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:88263 Capn1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002076961 – 713Calpain-1 catalytic subunitAdd BLAST713

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei354PhosphothreonineBy similarity1

Post-translational modificationi

Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei15 – 16Cleavage; for 78 kDa formBy similarity2
Sitei27 – 28Cleavage; for 75 kDa formBy similarity2

Keywords - PTMi

Autocatalytic cleavage, Phosphoprotein

Proteomic databases

EPDiO35350
PaxDbiO35350
PeptideAtlasiO35350
PRIDEiO35350

PTM databases

iPTMnetiO35350
PhosphoSitePlusiO35350
SwissPalmiO35350

Expressioni

Gene expression databases

BgeeiENSMUSG00000024942
CleanExiMM_CAPN1
ExpressionAtlasiO35350 baseline and differential
GenevisibleiO35350 MM

Interactioni

Subunit structurei

Forms a heterodimer with a small (regulatory) subunit CAPNS1.By similarity

Protein-protein interaction databases

BioGridi1984703 interactors.
DIPiDIP-60303N
IntActiO35350 5 interactors.
MINTiO35350
STRINGi10090.ENSMUSP00000025891

Structurei

3D structure databases

ProteinModelPortaliO35350
SMRiO35350
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 354Calpain catalyticPROSITE-ProRule annotationAdd BLAST300
Domaini540 – 575EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini584 – 617EF-hand 2PROSITE-ProRule annotationAdd BLAST34
Domaini614 – 649EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini679 – 713EF-hand 4PROSITE-ProRule annotationAdd BLAST35

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni355 – 525Domain IIIAdd BLAST171
Regioni526 – 541LinkerAdd BLAST16
Regioni542 – 712Domain IVAdd BLAST171

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0045 Eukaryota
ENOG410XP0B LUCA
GeneTreeiENSGT00760000118971
HOGENOMiHOG000232035
HOVERGENiHBG012645
InParanoidiO35350
KOiK01367
OMAiKWNTTLY
OrthoDBiEOG091G049E
PhylomeDBiO35350
TreeFamiTF314748

Family and domain databases

CDDicd00214 Calpain_III, 1 hit
cd00044 CysPc, 1 hit
InterProiView protein in InterPro
IPR033883 C2_III
IPR022684 Calpain_cysteine_protease
IPR022682 Calpain_domain_III
IPR022683 Calpain_III
IPR036213 Calpain_III_sf
IPR029643 CAPN1
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR000169 Pept_cys_AS
IPR001300 Peptidase_C2_calpain_cat
PANTHERiPTHR10183:SF284 PTHR10183:SF284, 1 hit
PfamiView protein in Pfam
PF01067 Calpain_III, 1 hit
PF13833 EF-hand_8, 1 hit
PF00648 Peptidase_C2, 1 hit
PRINTSiPR00704 CALPAIN
SMARTiView protein in SMART
SM00720 calpain_III, 1 hit
SM00230 CysPc, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF49758 SSF49758, 1 hit
PROSITEiView protein in PROSITE
PS50203 CALPAIN_CAT, 1 hit
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 4 hits
PS00139 THIOL_PROTEASE_CYS, 1 hit

Sequencei

Sequence statusi: Complete.

O35350-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEELITPVY CTGVSAQVQK KRDKELGLGR HENAIKYLGQ DYETLRARCL
60 70 80 90 100
QSGVLFQDEA FPPVSHSLGF KELGPHSSKT YGIKWKRPTE LMSNPQFIVD
110 120 130 140 150
GATRTDICQG ALGDCWLLAA IASLTLNETI LHRVVPYGQS FQDGYAGIFH
160 170 180 190 200
FQLWQFGEWV DVVIDDLLPT KDGKLVFVHS AQGNEFWSAL LEKAYAKVNG
210 220 230 240 250
SYEALSGGCT SEAFEDFTGG VTEWYDLQKA PSDLYQIILK ALERGSLLGC
260 270 280 290 300
SINISDIRDL EAITFKNLVR GHAYSVTGAK QVTYQGQRVN LIRMRNPWGE
310 320 330 340 350
VEWKGPWSDS SYEWNKVDPY EREQLRVKME DGEFWMSFRD FIREFTKLEI
360 370 380 390 400
CNLTPDALKS RTLRNWNTTF YEGTWRRGST AGGCRNYPAT FWVNPQFKIR
410 420 430 440 450
LEEVDDADDY DNRESGCSFL LALMQKHRRR ERRFGRDMET IGFAVYQVPR
460 470 480 490 500
ELAGQPVHLK RDFFLANASR AQSEHFINLR EVSNRIRLPP GEYIVVPSTF
510 520 530 540 550
EPNKEGDFLL RFFSEKKAGT QELDDQIQAN LPDEKVLSEE EIDDNFKTLF
560 570 580 590 600
SKLAGDDMEI SVKELQTILN RIISKHKDLR TNGFSLESCR SMVNLMDRDG
610 620 630 640 650
NGKLGLVEFN ILWNRIRNYL TIFRKFDLDK SGSMSAYEMR MAIEAAGFKL
660 670 680 690 700
NKKLHELIIT RYSEPDLAVD FDNFVCCLVR LETMFRFFKL LDTDLDGVVT
710
FDLFKWLQLT MFA
Length:713
Mass (Da):82,106
Last modified:January 1, 1998 - v1
Checksum:i3E1E26C95802B864
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti488L → P in AAC33134 (Ref. 2) Curated1
Sequence conflicti696D → N in AAC33134 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF021847 mRNA Translation: AAB72222.1
AF084459 mRNA Translation: AAC33134.1
BC026138 mRNA Translation: AAH26138.1
CCDSiCCDS37893.1
RefSeqiNP_001103974.1, NM_001110504.1
NP_031626.1, NM_007600.3
UniGeneiMm.6221

Genome annotation databases

EnsembliENSMUST00000025891; ENSMUSP00000025891; ENSMUSG00000024942
ENSMUST00000164843; ENSMUSP00000127498; ENSMUSG00000024942
GeneIDi12333
KEGGimmu:12333
UCSCiuc008ggj.2 mouse

Similar proteinsi

Entry informationi

Entry nameiCAN1_MOUSE
AccessioniPrimary (citable) accession number: O35350
Secondary accession number(s): O88666
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 1, 1998
Last modified: February 28, 2018
This is version 174 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome