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Protein

Calpain-1 catalytic subunit

Gene

Capn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.By similarity

Catalytic activityi

Broad endopeptidase specificity.By similarity

Cofactori

Ca2+By similarityNote: Binds 4 Ca2+ ions.By similarity

Enzyme regulationi

Activated by micromolar concentrations of calcium and inhibited by calpastatin.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei115By similarity1
Active sitei272By similarity1
Active sitei296By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi99 – 1061PROSITE-ProRule annotation8
Calcium bindingi302 – 3332PROSITE-ProRule annotationAdd BLAST32
Calcium bindingi597 – 6083PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi627 – 6384PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

  • mammary gland involution Source: MGI
  • proteolysis Source: MGI
  • receptor catabolic process Source: MGI
  • regulation of catalytic activity Source: UniProtKB
  • self proteolysis Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1474228. Degradation of the extracellular matrix.
R-MMU-6798695. Neutrophil degranulation.
R-MMU-6809371. Formation of the cornified envelope.
R-MMU-8862803. Deregulated CDK5 triggers multiple neurodegenerative pathways in Alzheimer's disease models.

Protein family/group databases

MEROPSiC02.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-1 catalytic subunitCurated (EC:3.4.22.52By similarity)
Alternative name(s):
Calcium-activated neutral proteinase 1By similarity
Short name:
CANP 1By similarity
Calpain mu-typeBy similarity
Calpain-1 large subunitBy similarity
Micromolar-calpain
Short name:
muCANPBy similarity
Gene namesi
Name:Capn1Imported
Synonyms:Canp1, Capa1By similarity
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:88263. Capn1.

Subcellular locationi

  • Cytoplasm By similarity
  • Cell membrane By similarity

  • Note: Translocates to the plasma membrane upon Ca2+ binding.By similarity

GO - Cellular componenti

  • cytosol Source: MGI
  • extracellular exosome Source: MGI
  • focal adhesion Source: MGI
  • lysosome Source: MGI
  • membrane Source: MGI
  • mitochondrion Source: MGI
  • plasma membrane Source: UniProtKB

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002076962 – 713Calpain-1 catalytic subunitAdd BLAST712

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei354PhosphothreonineBy similarity1

Post-translational modificationi

Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei15 – 16Cleavage; for 78 kDa formBy similarity2
Sitei27 – 28Cleavage; for 75 kDa formBy similarity2

Keywords - PTMi

Autocatalytic cleavage, Phosphoprotein

Proteomic databases

EPDiO35350.
PaxDbiO35350.
PeptideAtlasiO35350.
PRIDEiO35350.

PTM databases

iPTMnetiO35350.
PhosphoSitePlusiO35350.
SwissPalmiO35350.

Expressioni

Gene expression databases

BgeeiENSMUSG00000024942.
CleanExiMM_CAPN1.
ExpressionAtlasiO35350. baseline and differential.
GenevisibleiO35350. MM.

Interactioni

Subunit structurei

Forms a heterodimer with a small (regulatory) subunit CAPNS1.By similarity

Protein-protein interaction databases

BioGridi198470. 2 interactors.
DIPiDIP-60303N.
IntActiO35350. 4 interactors.
MINTiMINT-4089782.
STRINGi10090.ENSMUSP00000025891.

Structurei

3D structure databases

ProteinModelPortaliO35350.
SMRiO35350.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 354Calpain catalyticPROSITE-ProRule annotationAdd BLAST300
Domaini540 – 575EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini584 – 617EF-hand 2PROSITE-ProRule annotationAdd BLAST34
Domaini614 – 649EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini679 – 713EF-hand 4PROSITE-ProRule annotationAdd BLAST35

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni355 – 525Domain IIIAdd BLAST171
Regioni526 – 541LinkerAdd BLAST16
Regioni542 – 712Domain IVAdd BLAST171

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiO35350.
KOiK01367.
OMAiKWNTTLY.
OrthoDBiEOG091G049E.
PhylomeDBiO35350.
TreeFamiTF314748.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
cd00044. CysPc. 1 hit.
cd00051. EFh. 1 hit.
InterProiView protein in InterPro
IPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029643. CAPN1.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
PANTHERiPTHR10183:SF343. PTHR10183:SF343. 1 hit.
PfamiView protein in Pfam
PF01067. Calpain_III. 1 hit.
PF13833. EF-hand_8. 1 hit.
PF00648. Peptidase_C2. 1 hit.
PRINTSiPR00704. CALPAIN.
SMARTiView protein in SMART
SM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiView protein in PROSITE
PS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35350-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEELITPVY CTGVSAQVQK KRDKELGLGR HENAIKYLGQ DYETLRARCL
60 70 80 90 100
QSGVLFQDEA FPPVSHSLGF KELGPHSSKT YGIKWKRPTE LMSNPQFIVD
110 120 130 140 150
GATRTDICQG ALGDCWLLAA IASLTLNETI LHRVVPYGQS FQDGYAGIFH
160 170 180 190 200
FQLWQFGEWV DVVIDDLLPT KDGKLVFVHS AQGNEFWSAL LEKAYAKVNG
210 220 230 240 250
SYEALSGGCT SEAFEDFTGG VTEWYDLQKA PSDLYQIILK ALERGSLLGC
260 270 280 290 300
SINISDIRDL EAITFKNLVR GHAYSVTGAK QVTYQGQRVN LIRMRNPWGE
310 320 330 340 350
VEWKGPWSDS SYEWNKVDPY EREQLRVKME DGEFWMSFRD FIREFTKLEI
360 370 380 390 400
CNLTPDALKS RTLRNWNTTF YEGTWRRGST AGGCRNYPAT FWVNPQFKIR
410 420 430 440 450
LEEVDDADDY DNRESGCSFL LALMQKHRRR ERRFGRDMET IGFAVYQVPR
460 470 480 490 500
ELAGQPVHLK RDFFLANASR AQSEHFINLR EVSNRIRLPP GEYIVVPSTF
510 520 530 540 550
EPNKEGDFLL RFFSEKKAGT QELDDQIQAN LPDEKVLSEE EIDDNFKTLF
560 570 580 590 600
SKLAGDDMEI SVKELQTILN RIISKHKDLR TNGFSLESCR SMVNLMDRDG
610 620 630 640 650
NGKLGLVEFN ILWNRIRNYL TIFRKFDLDK SGSMSAYEMR MAIEAAGFKL
660 670 680 690 700
NKKLHELIIT RYSEPDLAVD FDNFVCCLVR LETMFRFFKL LDTDLDGVVT
710
FDLFKWLQLT MFA
Length:713
Mass (Da):82,106
Last modified:January 1, 1998 - v1
Checksum:i3E1E26C95802B864
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti488L → P in AAC33134 (Ref. 2) Curated1
Sequence conflicti696D → N in AAC33134 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF021847 mRNA. Translation: AAB72222.1.
AF084459 mRNA. Translation: AAC33134.1.
BC026138 mRNA. Translation: AAH26138.1.
CCDSiCCDS37893.1.
RefSeqiNP_001103974.1. NM_001110504.1.
NP_031626.1. NM_007600.3.
UniGeneiMm.6221.

Genome annotation databases

EnsembliENSMUST00000025891; ENSMUSP00000025891; ENSMUSG00000024942.
ENSMUST00000164843; ENSMUSP00000127498; ENSMUSG00000024942.
GeneIDi12333.
KEGGimmu:12333.
UCSCiuc008ggj.2. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiCAN1_MOUSE
AccessioniPrimary (citable) accession number: O35350
Secondary accession number(s): O88666
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 1, 1998
Last modified: June 7, 2017
This is version 169 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families