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O35350

- CAN1_MOUSE

UniProt

O35350 - CAN1_MOUSE

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Protein

Calpain-1 catalytic subunit

Gene

Capn1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Ca2+By similarityNote: Binds 4 Ca(2+) ions.By similarity

Enzyme regulationi

Activated by micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei15 – 162Cleavage; for 78 kDa formBy similarity
Sitei27 – 282Cleavage; for 75 kDa formBy similarity
Active sitei115 – 1151By similarity
Active sitei272 – 2721By similarity
Active sitei296 – 2961By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi99 – 10681PROSITE-ProRule annotation
Calcium bindingi302 – 333322PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi597 – 608123PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi627 – 638124PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. calcium-dependent cysteine-type endopeptidase activity Source: MGI
  2. calcium ion binding Source: InterPro

GO - Biological processi

  1. proteolysis Source: UniProtKB
  2. receptor catabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_199052. Degradation of the extracellular matrix.

Protein family/group databases

MEROPSiC02.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-1 catalytic subunit (EC:3.4.22.52)
Alternative name(s):
Calcium-activated neutral proteinase 1
Short name:
CANP 1
Calpain mu-type
Calpain-1 large subunit
Micromolar-calpain
Short name:
muCANP
Gene namesi
Name:Capn1
Synonyms:Canp1, Capa1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:88263. Capn1.

Subcellular locationi

Cytoplasm By similarity. Cell membrane By similarity
Note: Translocates to the plasma membrane upon Ca2+ binding.By similarity

GO - Cellular componenti

  1. cytoplasm Source: RefGenome
  2. cytosol Source: Ensembl
  3. extracellular vesicular exosome Source: Ensembl
  4. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 713712Calpain-1 catalytic subunitPRO_0000207696Add
BLAST

Post-translational modificationi

Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms (By similarity).By similarity

Keywords - PTMi

Autocatalytic cleavage

Proteomic databases

MaxQBiO35350.
PaxDbiO35350.
PRIDEiO35350.

PTM databases

PhosphoSiteiO35350.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiO35350.
CleanExiMM_CAPN1.
ExpressionAtlasiO35350. baseline and differential.
GenevestigatoriO35350.

Interactioni

Subunit structurei

Forms a heterodimer with a small (regulatory) subunit (CAPNS1).

Protein-protein interaction databases

BioGridi198470. 2 interactions.
DIPiDIP-60303N.
IntActiO35350. 2 interactions.
MINTiMINT-4089782.

Structurei

3D structure databases

ProteinModelPortaliO35350.
SMRiO35350. Positions 13-712.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini55 – 354300Calpain catalyticPROSITE-ProRule annotationAdd
BLAST
Domaini540 – 57536EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini584 – 61734EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini614 – 64936EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini679 – 71335EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni355 – 525171Domain IIIAdd
BLAST
Regioni526 – 54116LinkerAdd
BLAST
Regioni542 – 712171Domain IVAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG327523.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiO35350.
KOiK01367.
OMAiPQSLGYK.
OrthoDBiEOG7RV9FM.
PhylomeDBiO35350.
TreeFamiTF314748.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029643. CAPN1.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF284. PTHR10183:SF284. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35350-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTEELITPVY CTGVSAQVQK KRDKELGLGR HENAIKYLGQ DYETLRARCL
60 70 80 90 100
QSGVLFQDEA FPPVSHSLGF KELGPHSSKT YGIKWKRPTE LMSNPQFIVD
110 120 130 140 150
GATRTDICQG ALGDCWLLAA IASLTLNETI LHRVVPYGQS FQDGYAGIFH
160 170 180 190 200
FQLWQFGEWV DVVIDDLLPT KDGKLVFVHS AQGNEFWSAL LEKAYAKVNG
210 220 230 240 250
SYEALSGGCT SEAFEDFTGG VTEWYDLQKA PSDLYQIILK ALERGSLLGC
260 270 280 290 300
SINISDIRDL EAITFKNLVR GHAYSVTGAK QVTYQGQRVN LIRMRNPWGE
310 320 330 340 350
VEWKGPWSDS SYEWNKVDPY EREQLRVKME DGEFWMSFRD FIREFTKLEI
360 370 380 390 400
CNLTPDALKS RTLRNWNTTF YEGTWRRGST AGGCRNYPAT FWVNPQFKIR
410 420 430 440 450
LEEVDDADDY DNRESGCSFL LALMQKHRRR ERRFGRDMET IGFAVYQVPR
460 470 480 490 500
ELAGQPVHLK RDFFLANASR AQSEHFINLR EVSNRIRLPP GEYIVVPSTF
510 520 530 540 550
EPNKEGDFLL RFFSEKKAGT QELDDQIQAN LPDEKVLSEE EIDDNFKTLF
560 570 580 590 600
SKLAGDDMEI SVKELQTILN RIISKHKDLR TNGFSLESCR SMVNLMDRDG
610 620 630 640 650
NGKLGLVEFN ILWNRIRNYL TIFRKFDLDK SGSMSAYEMR MAIEAAGFKL
660 670 680 690 700
NKKLHELIIT RYSEPDLAVD FDNFVCCLVR LETMFRFFKL LDTDLDGVVT
710
FDLFKWLQLT MFA
Length:713
Mass (Da):82,106
Last modified:January 1, 1998 - v1
Checksum:i3E1E26C95802B864
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti488 – 4881L → P in AAC33134. 1 PublicationCurated
Sequence conflicti696 – 6961D → N in AAC33134. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF021847 mRNA. Translation: AAB72222.1.
AF084459 mRNA. Translation: AAC33134.1.
BC026138 mRNA. Translation: AAH26138.1.
CCDSiCCDS37893.1.
RefSeqiNP_001103974.1. NM_001110504.1.
NP_031626.1. NM_007600.3.
UniGeneiMm.6221.

Genome annotation databases

EnsembliENSMUST00000025891; ENSMUSP00000025891; ENSMUSG00000024942.
ENSMUST00000164843; ENSMUSP00000127498; ENSMUSG00000024942.
GeneIDi12333.
KEGGimmu:12333.
UCSCiuc008ggj.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF021847 mRNA. Translation: AAB72222.1 .
AF084459 mRNA. Translation: AAC33134.1 .
BC026138 mRNA. Translation: AAH26138.1 .
CCDSi CCDS37893.1.
RefSeqi NP_001103974.1. NM_001110504.1.
NP_031626.1. NM_007600.3.
UniGenei Mm.6221.

3D structure databases

ProteinModelPortali O35350.
SMRi O35350. Positions 13-712.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198470. 2 interactions.
DIPi DIP-60303N.
IntActi O35350. 2 interactions.
MINTi MINT-4089782.

Protein family/group databases

MEROPSi C02.001.

PTM databases

PhosphoSitei O35350.

Proteomic databases

MaxQBi O35350.
PaxDbi O35350.
PRIDEi O35350.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000025891 ; ENSMUSP00000025891 ; ENSMUSG00000024942 .
ENSMUST00000164843 ; ENSMUSP00000127498 ; ENSMUSG00000024942 .
GeneIDi 12333.
KEGGi mmu:12333.
UCSCi uc008ggj.2. mouse.

Organism-specific databases

CTDi 823.
MGIi MGI:88263. Capn1.

Phylogenomic databases

eggNOGi NOG327523.
HOGENOMi HOG000232035.
HOVERGENi HBG012645.
InParanoidi O35350.
KOi K01367.
OMAi PQSLGYK.
OrthoDBi EOG7RV9FM.
PhylomeDBi O35350.
TreeFami TF314748.

Enzyme and pathway databases

Reactomei REACT_199052. Degradation of the extracellular matrix.

Miscellaneous databases

NextBioi 280940.
PROi O35350.
SOURCEi Search...

Gene expression databases

Bgeei O35350.
CleanExi MM_CAPN1.
ExpressionAtlasi O35350. baseline and differential.
Genevestigatori O35350.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
InterProi IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029643. CAPN1.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view ]
PANTHERi PTHR10183:SF284. PTHR10183:SF284. 1 hit.
Pfami PF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view ]
PRINTSi PR00704. CALPAIN.
SMARTi SM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 3 hits.
[Graphical view ]
SUPFAMi SSF49758. SSF49758. 1 hit.
PROSITEi PS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Poirier C., Poussard S., Faust D.M., Imaizumi-Sherrer T., Weiss M.C., Ducastaing A., Montarras D., Pinset C., Guenet J.-L.
    Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: CF-1.
  2. "Cloning and characterization of the cDNA and gene encoding the mouse mu-calpain large subunit protein."
    Sahr K.E., Andrabi S., Peters L.L., Chishti A.H.
    Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 129.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon.
  4. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiCAN1_MOUSE
AccessioniPrimary (citable) accession number: O35350
Secondary accession number(s): O88666
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 1, 1998
Last modified: November 26, 2014
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3