Calpain-1 catalytic subunit - Mus musculus (Mouse)

Contribute

Send feedback

Read comments (?) or add your own

O35350 (CAN1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 129. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Calpain-1 catalytic subunit
EC=3.4.22.52

Alternative name(s):
Calcium-activated neutral proteinase 1
Short name=CANP 1
Calpain mu-type
Calpain-1 large subunit
Micromolar-calpain
Short name=muCANP

Gene names

Name:	Capn1
Synonyms:	Canp1, Capa1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	713 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Catalytic activity	Broad endopeptidase specificity.
Cofactor	Binds 4 calcium ions By similarity.
Enzyme regulation	Activated by micromolar concentrations of calcium and inhibited by calpastatin.
Subunit structure	Forms a heterodimer with a small (regulatory) subunit (CAPNS1).
Subcellular location	Cytoplasm By similarity. Cell membrane By similarity. Note: Translocates to the plasma membrane upon Ca²⁺ binding By similarity.
Tissue specificity	Ubiquitous.
Post-translational modification	Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms By similarity.
Sequence similarities	Belongs to the peptidase C2 family. Contains 1 calpain catalytic domain. Contains 4 EF-hand domains.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Membrane
Domain	Repeat
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Thiol protease
PTM	Autocatalytic cleavage
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW receptor catabolic process Inferred from direct assay PubMed 17529984. Source: MGI
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro calcium-dependent cysteine-type endopeptidase activity Inferred from direct assay PubMed 17529984. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 713

713

Calpain-1 catalytic subunit

PRO_0000207696

Regions

Domain

55 – 354

300

Calpain catalytic

Domain

540 – 575

EF-hand 1

Domain

584 – 617

EF-hand 2

Domain

614 – 649

EF-hand 3

Domain

679 – 713

EF-hand 4

Calcium binding

99 – 106

1 By similarity

Calcium binding

302 – 333

2 By similarity

Calcium binding

597 – 608

3 By similarity

Calcium binding

627 – 638

4 By similarity

Region

355 – 525

171

Domain III

Region

526 – 541

Linker

Region

542 – 712

171

Domain IV

Sites

Active site

115

By similarity

Active site

272

By similarity

Active site

296

By similarity

Site

15 – 16

Cleavage; for 78 kDa form By similarity

Site

27 – 28

Cleavage; for 75 kDa form By similarity

Experimental info

Sequence conflict

488

L → P in AAC33134. Ref.2

Sequence conflict

696

D → N in AAC33134. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

O35350 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 3E1E26C95802B864
Show »

FASTA

713

82,106

        10         20         30         40         50         60 
MTEELITPVY CTGVSAQVQK KRDKELGLGR HENAIKYLGQ DYETLRARCL QSGVLFQDEA 

        70         80         90        100        110        120 
FPPVSHSLGF KELGPHSSKT YGIKWKRPTE LMSNPQFIVD GATRTDICQG ALGDCWLLAA 

       130        140        150        160        170        180 
IASLTLNETI LHRVVPYGQS FQDGYAGIFH FQLWQFGEWV DVVIDDLLPT KDGKLVFVHS 

       190        200        210        220        230        240 
AQGNEFWSAL LEKAYAKVNG SYEALSGGCT SEAFEDFTGG VTEWYDLQKA PSDLYQIILK 

       250        260        270        280        290        300 
ALERGSLLGC SINISDIRDL EAITFKNLVR GHAYSVTGAK QVTYQGQRVN LIRMRNPWGE 

       310        320        330        340        350        360 
VEWKGPWSDS SYEWNKVDPY EREQLRVKME DGEFWMSFRD FIREFTKLEI CNLTPDALKS 

       370        380        390        400        410        420 
RTLRNWNTTF YEGTWRRGST AGGCRNYPAT FWVNPQFKIR LEEVDDADDY DNRESGCSFL 

       430        440        450        460        470        480 
LALMQKHRRR ERRFGRDMET IGFAVYQVPR ELAGQPVHLK RDFFLANASR AQSEHFINLR 

       490        500        510        520        530        540 
EVSNRIRLPP GEYIVVPSTF EPNKEGDFLL RFFSEKKAGT QELDDQIQAN LPDEKVLSEE 

       550        560        570        580        590        600 
EIDDNFKTLF SKLAGDDMEI SVKELQTILN RIISKHKDLR TNGFSLESCR SMVNLMDRDG 

       610        620        630        640        650        660 
NGKLGLVEFN ILWNRIRNYL TIFRKFDLDK SGSMSAYEMR MAIEAAGFKL NKKLHELIIT 

       670        680        690        700        710 
RYSEPDLAVD FDNFVCCLVR LETMFRFFKL LDTDLDGVVT FDLFKWLQLT MFA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Poirier C., Poussard S., Faust D.M., Imaizumi-Sherrer T., Weiss M.C., Ducastaing A., Montarras D., Pinset C., Guenet J.-L. Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: CF-1.
[2]	"Cloning and characterization of the cDNA and gene encoding the mouse mu-calpain large subunit protein." Sahr K.E., Andrabi S., Peters L.L., Chishti A.H. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 129.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Colon.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF021847 mRNA. Translation: AAB72222.1. AF084459 mRNA. Translation: AAC33134.1. BC026138 mRNA. Translation: AAH26138.1.
IPI	IPI00626909.
RefSeq	NP_001103974.1. NM_001110504.1. NP_031626.1. NM_007600.3.
UniGene	Mm.6221.
3D structure databases
ProteinModelPortal	O35350.
SMR	O35350. Positions 13-712.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4089782.
Protein family/group databases
MEROPS	C02.001.
PTM databases
PhosphoSite	O35350.
Proteomic databases
PaxDb	O35350.
PRIDE	O35350.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000025891; ENSMUSP00000025891; ENSMUSG00000024942. ENSMUST00000164843; ENSMUSP00000127498; ENSMUSG00000024942.
GeneID	12333.
KEGG	mmu:12333.
UCSC	uc008ggj.2. mouse.
Organism-specific databases
CTD	823.
MGI	MGI:88263. Capn1.
Phylogenomic databases
eggNOG	NOG327523.
HOGENOM	HOG000232035.
HOVERGEN	HBG012645.
InParanoid	O35350.
KO	K01367.
OMA	DFLREFS.
OrthoDB	EOG4RR6GS.
Gene expression databases
ArrayExpress	O35350.
Bgee	O35350.
CleanEx	MM_CAPN1.
Genevestigator	O35350.
GermOnline	ENSMUSG00000024942. Mus musculus.
Family and domain databases
Gene3D	1.10.238.10. 1 hit.
InterPro	IPR022684. Calpain_cysteine_protease. IPR022682. Calpain_domain_III. IPR022683. Calpain_III. IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. IPR000169. Pept_cys_AS. IPR001300. Peptidase_C2_calpain_cat. [Graphical view]
Pfam	PF01067. Calpain_III. 1 hit. PF00648. Peptidase_C2. 1 hit. [Graphical view]
PRINTS	PR00704. CALPAIN.
SMART	SM00720. calpain_III. 1 hit. SM00230. CysPc. 1 hit. SM00054. EFh. 3 hits. [Graphical view]
SUPFAM	SSF49758. Peptidase_C2. 1 hit.
PROSITE	PS50203. CALPAIN_CAT. 1 hit. PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 4 hits. PS00640. THIOL_PROTEASE_ASN. False negative. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. False negative. [Graphical view]
ProtoNet	Search...
Other
BindingDB	O35350.
ChEMBL	CHEMBL4210.
NextBio	280940.
SOURCE	Search...

Entry information

Entry name

CAN1_MOUSE

Accession

Primary (citable) accession number: O35350
Secondary accession number(s): O88666

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	January 1, 1998
Last modified:	May 29, 2013
This is version 129 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents