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O35350

- CAN1_MOUSE

UniProt

O35350 - CAN1_MOUSE

Protein

Calpain-1 catalytic subunit

Gene

Capn1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 142 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

    Catalytic activityi

    Broad endopeptidase specificity.

    Cofactori

    Binds 4 calcium ions.By similarity

    Enzyme regulationi

    Activated by micromolar concentrations of calcium and inhibited by calpastatin.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei15 – 162Cleavage; for 78 kDa formBy similarity
    Sitei27 – 282Cleavage; for 75 kDa formBy similarity
    Active sitei115 – 1151By similarity
    Active sitei272 – 2721By similarity
    Active sitei296 – 2961By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi99 – 10681PROSITE-ProRule annotation
    Calcium bindingi302 – 333322PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi597 – 608123PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi627 – 638124PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium-dependent cysteine-type endopeptidase activity Source: MGI
    2. calcium ion binding Source: InterPro
    3. protein binding Source: MGI

    GO - Biological processi

    1. proteolysis Source: UniProtKB
    2. receptor catabolic process Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_199052. Degradation of the extracellular matrix.

    Protein family/group databases

    MEROPSiC02.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calpain-1 catalytic subunit (EC:3.4.22.52)
    Alternative name(s):
    Calcium-activated neutral proteinase 1
    Short name:
    CANP 1
    Calpain mu-type
    Calpain-1 large subunit
    Micromolar-calpain
    Short name:
    muCANP
    Gene namesi
    Name:Capn1
    Synonyms:Canp1, Capa1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:88263. Capn1.

    Subcellular locationi

    Cytoplasm By similarity. Cell membrane By similarity
    Note: Translocates to the plasma membrane upon Ca2+ binding.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: RefGenome
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 713712Calpain-1 catalytic subunitPRO_0000207696Add
    BLAST

    Post-translational modificationi

    Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms By similarity.By similarity

    Keywords - PTMi

    Autocatalytic cleavage

    Proteomic databases

    MaxQBiO35350.
    PaxDbiO35350.
    PRIDEiO35350.

    PTM databases

    PhosphoSiteiO35350.

    Expressioni

    Tissue specificityi

    Ubiquitous.

    Gene expression databases

    ArrayExpressiO35350.
    BgeeiO35350.
    CleanExiMM_CAPN1.
    GenevestigatoriO35350.

    Interactioni

    Subunit structurei

    Forms a heterodimer with a small (regulatory) subunit (CAPNS1).

    Protein-protein interaction databases

    BioGridi198470. 2 interactions.
    DIPiDIP-60303N.
    IntActiO35350. 2 interactions.
    MINTiMINT-4089782.

    Structurei

    3D structure databases

    ProteinModelPortaliO35350.
    SMRiO35350. Positions 13-712.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini55 – 354300Calpain catalyticPROSITE-ProRule annotationAdd
    BLAST
    Domaini540 – 57536EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini584 – 61734EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini614 – 64936EF-hand 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini679 – 71335EF-hand 4PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni355 – 525171Domain IIIAdd
    BLAST
    Regioni526 – 54116LinkerAdd
    BLAST
    Regioni542 – 712171Domain IVAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C2 family.Curated
    Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
    Contains 4 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG327523.
    HOGENOMiHOG000232035.
    HOVERGENiHBG012645.
    InParanoidiO35350.
    KOiK01367.
    OMAiPQSLGYK.
    OrthoDBiEOG7RV9FM.
    PhylomeDBiO35350.
    TreeFamiTF314748.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR022684. Calpain_cysteine_protease.
    IPR022682. Calpain_domain_III.
    IPR022683. Calpain_III.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000169. Pept_cys_AS.
    IPR001300. Peptidase_C2_calpain_cat.
    [Graphical view]
    PfamiPF01067. Calpain_III. 1 hit.
    PF00648. Peptidase_C2. 1 hit.
    [Graphical view]
    PRINTSiPR00704. CALPAIN.
    SMARTiSM00720. calpain_III. 1 hit.
    SM00230. CysPc. 1 hit.
    SM00054. EFh. 3 hits.
    [Graphical view]
    SUPFAMiSSF49758. SSF49758. 1 hit.
    PROSITEiPS50203. CALPAIN_CAT. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 4 hits.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O35350-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTEELITPVY CTGVSAQVQK KRDKELGLGR HENAIKYLGQ DYETLRARCL    50
    QSGVLFQDEA FPPVSHSLGF KELGPHSSKT YGIKWKRPTE LMSNPQFIVD 100
    GATRTDICQG ALGDCWLLAA IASLTLNETI LHRVVPYGQS FQDGYAGIFH 150
    FQLWQFGEWV DVVIDDLLPT KDGKLVFVHS AQGNEFWSAL LEKAYAKVNG 200
    SYEALSGGCT SEAFEDFTGG VTEWYDLQKA PSDLYQIILK ALERGSLLGC 250
    SINISDIRDL EAITFKNLVR GHAYSVTGAK QVTYQGQRVN LIRMRNPWGE 300
    VEWKGPWSDS SYEWNKVDPY EREQLRVKME DGEFWMSFRD FIREFTKLEI 350
    CNLTPDALKS RTLRNWNTTF YEGTWRRGST AGGCRNYPAT FWVNPQFKIR 400
    LEEVDDADDY DNRESGCSFL LALMQKHRRR ERRFGRDMET IGFAVYQVPR 450
    ELAGQPVHLK RDFFLANASR AQSEHFINLR EVSNRIRLPP GEYIVVPSTF 500
    EPNKEGDFLL RFFSEKKAGT QELDDQIQAN LPDEKVLSEE EIDDNFKTLF 550
    SKLAGDDMEI SVKELQTILN RIISKHKDLR TNGFSLESCR SMVNLMDRDG 600
    NGKLGLVEFN ILWNRIRNYL TIFRKFDLDK SGSMSAYEMR MAIEAAGFKL 650
    NKKLHELIIT RYSEPDLAVD FDNFVCCLVR LETMFRFFKL LDTDLDGVVT 700
    FDLFKWLQLT MFA 713
    Length:713
    Mass (Da):82,106
    Last modified:January 1, 1998 - v1
    Checksum:i3E1E26C95802B864
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti488 – 4881L → P in AAC33134. 1 PublicationCurated
    Sequence conflicti696 – 6961D → N in AAC33134. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF021847 mRNA. Translation: AAB72222.1.
    AF084459 mRNA. Translation: AAC33134.1.
    BC026138 mRNA. Translation: AAH26138.1.
    CCDSiCCDS37893.1.
    RefSeqiNP_001103974.1. NM_001110504.1.
    NP_031626.1. NM_007600.3.
    UniGeneiMm.6221.

    Genome annotation databases

    EnsembliENSMUST00000025891; ENSMUSP00000025891; ENSMUSG00000024942.
    ENSMUST00000164843; ENSMUSP00000127498; ENSMUSG00000024942.
    GeneIDi12333.
    KEGGimmu:12333.
    UCSCiuc008ggj.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF021847 mRNA. Translation: AAB72222.1 .
    AF084459 mRNA. Translation: AAC33134.1 .
    BC026138 mRNA. Translation: AAH26138.1 .
    CCDSi CCDS37893.1.
    RefSeqi NP_001103974.1. NM_001110504.1.
    NP_031626.1. NM_007600.3.
    UniGenei Mm.6221.

    3D structure databases

    ProteinModelPortali O35350.
    SMRi O35350. Positions 13-712.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198470. 2 interactions.
    DIPi DIP-60303N.
    IntActi O35350. 2 interactions.
    MINTi MINT-4089782.

    Chemistry

    BindingDBi O35350.

    Protein family/group databases

    MEROPSi C02.001.

    PTM databases

    PhosphoSitei O35350.

    Proteomic databases

    MaxQBi O35350.
    PaxDbi O35350.
    PRIDEi O35350.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000025891 ; ENSMUSP00000025891 ; ENSMUSG00000024942 .
    ENSMUST00000164843 ; ENSMUSP00000127498 ; ENSMUSG00000024942 .
    GeneIDi 12333.
    KEGGi mmu:12333.
    UCSCi uc008ggj.2. mouse.

    Organism-specific databases

    CTDi 823.
    MGIi MGI:88263. Capn1.

    Phylogenomic databases

    eggNOGi NOG327523.
    HOGENOMi HOG000232035.
    HOVERGENi HBG012645.
    InParanoidi O35350.
    KOi K01367.
    OMAi PQSLGYK.
    OrthoDBi EOG7RV9FM.
    PhylomeDBi O35350.
    TreeFami TF314748.

    Enzyme and pathway databases

    Reactomei REACT_199052. Degradation of the extracellular matrix.

    Miscellaneous databases

    NextBioi 280940.
    PROi O35350.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O35350.
    Bgeei O35350.
    CleanExi MM_CAPN1.
    Genevestigatori O35350.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR022684. Calpain_cysteine_protease.
    IPR022682. Calpain_domain_III.
    IPR022683. Calpain_III.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000169. Pept_cys_AS.
    IPR001300. Peptidase_C2_calpain_cat.
    [Graphical view ]
    Pfami PF01067. Calpain_III. 1 hit.
    PF00648. Peptidase_C2. 1 hit.
    [Graphical view ]
    PRINTSi PR00704. CALPAIN.
    SMARTi SM00720. calpain_III. 1 hit.
    SM00230. CysPc. 1 hit.
    SM00054. EFh. 3 hits.
    [Graphical view ]
    SUPFAMi SSF49758. SSF49758. 1 hit.
    PROSITEi PS50203. CALPAIN_CAT. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 4 hits.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Poirier C., Poussard S., Faust D.M., Imaizumi-Sherrer T., Weiss M.C., Ducastaing A., Montarras D., Pinset C., Guenet J.-L.
      Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: CF-1.
    2. "Cloning and characterization of the cDNA and gene encoding the mouse mu-calpain large subunit protein."
      Sahr K.E., Andrabi S., Peters L.L., Chishti A.H.
      Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: 129.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Colon.
    4. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.

    Entry informationi

    Entry nameiCAN1_MOUSE
    AccessioniPrimary (citable) accession number: O35350
    Secondary accession number(s): O88666
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 142 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3