Reviewed, UniProtKB/Swiss-Prot O35348 (COLQ_MOUSE)

Names and origin

Protein names

Recommended name:
    Acetylcholinesterase collagenic tail peptide
Alternative name(s):
    AChE Q subunit
    Acetylcholinesterase-associated collagen

Gene names

Name:

Colq

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Anchors the catalytic subunits of asymmetric AChE to the synaptic basal lamina By similarity.
Subunit structure	Homotrimer. Component of the asymmetric form of AChE, a disulfide-bonded oligomer composed of the collagenic subunits (Q) and a variable number of asymmetric catalytic subunits (T). The N-terminal of a collagenic subunit (Q) associates with the C-terminal of a catalytic subunit (T) By similarity.
Subcellular location	Cell junction › synapse By similarity.
Domain	The proline-rich attachment domain (PRAD) binds the AChE catalytic subunits By similarity.
Post-translational modification	The triple-helical tail is stabilized by disulfide bonds at each end By similarity.
Sequence similarities	Belongs to the COLQ family. Contains 2 collagen-like domains.

Ontologies

Keywords
Biological process	Neurotransmitter degradation
Cellular component	Cell junction Synapse
Domain	Collagen Repeat Signal
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	neurotransmitter catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW synapse Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 457	428	Acetylcholinesterase collagenic tail peptide		PRO_0000059415
Regions
	Domain	95 – 154	60	Collagen-like 1
	Domain	158 – 271	114	Collagen-like 2
	Region	51 – 67	17	PRAD By similarity
	Region	129 – 132	4	Heparan sulfate proteoglycan binding Potential
	Region	234 – 237	4	Heparan sulfate proteoglycan binding Potential
Amino acid modifications
	Disulfide bond	51		Interchain (with T subunit) Potential
	Disulfide bond	52		Interchain (with T subunit) Potential
	Disulfide bond	92		Interchain Potential
	Disulfide bond	293		Interchain Potential
	Disulfide bond	295		Interchain Potential
Experimental info
	Sequence conflict	105	1	Missing in AAB72195. Ref.2
	Sequence conflict	250	1	P → H in AAB72195. Ref.2
	Sequence conflict	267	1	P → A in AAB72195. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O35348-1 [UniParc]. Last modified January 9, 2007. Version 2. Checksum: 461E93C3DB0162B2	FASTA	457	47,684
	10 20 30 40 50 60 MVVLNPMTLG IYLQLFFCSI VSQPTFINSV VPISAALPGL DQKKRGSHKA CCLLMPPPPP 70 80 90 100 110 120 LFPPPFFRGS RSPLLSPDMK NLLELEASPS PCIQGSLGSP GPPGPQGPPG LPGKTGPKGE 130 140 150 160 170 180 KGDLGRPGRK GRPGPPGVPG MPGPVGWPGP EGPRGEKGDL GMMGLPGSRG PMGSKGFPGS 190 200 210 220 230 240 RGEKGSRGER GDLGPKGEKG FPGFPGMLGQ KGEMGPKGES GLAGHRGPTG RPGKRGKQGQ 250 260 270 280 290 300 KGDSGIMGPP GKPGPSGQPG RQGPPGPPGP PSAGQLVMGL KGERGFPGPP GRCLCGPPVN 310 320 330 340 350 360 VNNPSYGDSM YGRGSPRVPA IFVVNNQEEL ERLNTQNAIA FRRDQRSLYF KDSLGWLPIQ 370 380 390 400 410 420 LTPFYPVGYT VKQPGTCGDG VLQPGEECDD GNPDVSDGCI DCHRAYCGDG YRHQGVEDCD 430 440 450 GSDFGYLTCE TYLPGSYGDL RCTQYCSIDS TPCRYFT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"The mammalian gene of acetylcholinesterase-associated collagen." Krejci E., Thomine S., Boschetti N., Legay C., Sketelj J., Massoulie J. J. Biol. Chem. 272:22840-22847(1997) [PubMed: 9278446] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 37-273. Strain: 129/SvJ.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC107386 mRNA. Translation: AAI07387.1. AF021231 , AF015475, AF015476, AF015477, AF015478, AF015479, AF015480, AF015481, AF021230 Genomic DNA. Translation: AAB72195.1.
IPI	IPI00761778.
RefSeq	NP_034067.2.
UniGene	Mm.239821
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O35348.
Proteomic databases
PRIDE	O35348.
Genome annotation databases
Ensembl	ENSMUST00000112027; ENSMUSP00000107658; ENSMUSG00000057606; Mus musculus. [Genome view]
GeneID	382864.
KEGG	mmu:382864.
UCSC	uc007sxv.1. mouse.
Organism-specific databases
CTD	382864.
MGI	MGI:1338761. Colq.
Phylogenomic databases
HOGENOM	O35348.
HOVERGEN	O35348.
OMA	YPVDYTV
OrthoDB	EOG9M94B0
Gene expression databases
ArrayExpress	O35348.
Bgee	O35348.
Genevestigator	O35348.
GermOnline	ENSMUSG00000057606. Mus musculus.
Family and domain databases
InterPro	IPR008160. Collagen. IPR011936. Myxo_disulph_rpt. [Graphical view]
Pfam	PF01391. Collagen. 3 hits. [Graphical view]
TIGRFAMs	TIGR02232. myxo_disulf_rpt. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	403537.
SOURCE	Search...

Entry information

Entry name

COLQ_MOUSE

Accession

Primary (citable) accession number: O35348
Secondary accession number(s): Q08EK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	January 9, 2007
Last modified:	November 24, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)